Team:NAU-CHINA
Selenium has one of the narrowest ranges between dietary deficiency (<40 mg/day) and toxic levels (>400 mg /day) , leading to the unbalanced distribution of selenium in space.
To solve this problem, NAU-CHINA 2021 focuses on the selenium reduction and recycling through engineered bacteria to form selenium nano-particles (SeNPs), which is of eminent qualities in many fields.
In this project, we selected Escherichia coli BL21(DE3) as our chasis and mainly designed a genetic circuit containing two major modules, ssuE & sefA to achieve our goal.
Several convictive evidences has shown that we had accomplished our experiment and produced some prototype SeNPs fertilizer in lab-scale eventually. The subsequent pilot-scale and even industrial implementation are on the horizon.
Gene Circuit Stochastic model is built to find best promoter combinations and to calculate theoretical yield in laboratory environment, using tau-leaping algorithm. Then we add deterministic model when it comes to natural environment.
Response Surface Method is used to provide a theoretical basis for the best conditions for large-scale production of SeNPs.