Team:Manchester/Parts
This part encodes the sarcosine-responsive transcriptional regulator SouR, optimised for E. coli codon usage. SouR originates from Pseudomonas aeruginosa and is involved in activation of gene expression from the glyA1 promoter in the presence of sarcosine.
More information: Part Registry Page
This part encodes the sarcosine-inducible promoter PglyA1, originating from Pseudomonas aeruginosa. Activation of gene expression from the glyA1 promoter is controlled by the transcriptional regulator SouR in response to sarcosine.
More information: Part Registry Page
This part encodes the urea-responsive transcriptional regulator UreR, optimised for E. coli codon usage. UreR originates from Proteus mirabilis and is involved in activation of gene expression from the ureD promoter in the presence of urea.
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This part encodes the urea-inducible promoter PureD, originating from Proteus mirabilis. Activation of gene expression from the ureD promoter is controlled by the transcriptional regulator UreR in response to urea.
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This part harbours the urea biosensor composed of the urea-responsive transcriptional regulator UreR, optimised for E. coli codon usage, and its corresponding inducible promoter PureD linked to the rfp reporter gene.
More information: Part Registry Page
This part harbours the sarcosine biosensor composed of the sarcosine-responsive transcriptional regulator SouR, optimised for E. coli codon usage, and its corresponding inducible promoter PglyA1 linked to the rfp reporter gene.
More information: Part Registry Page
To bind our chassis to a cellulose-based hydrogel coating for urinary catheters, we designed the composite part LPP-OmpA-CBD(cex) (BBa_K863101) comprising the Cellulose Binding Domain (CBD) on the exoglucanase/xylanase (Cex) from Cellulomonas fimi (BBa_K863101) fused into an LPP-OmpA-N hybrid (BBa_K1694002).
More information: Part Registry Page