Contribution
There are more than 20,000 BioBricks in the iGEM Registry of Standard Biological Parts. These are the building blocks that teams may use to design and implement their proposed systems. This year we relied on a collection of 8 basic parts and created 8 composite parts. Based on the following basic parts, we designed our composite parts in SnapGene program. Our BioBricks’ order was executed by IDT and Twist Biosciences. In order to construct our composite parts, we used the NEBuilder HiFi DNA Assembly Master Mix.
BASIC PARTS
| Name |
Type |
Description |
Designer |
Length |
| BBa_K4056000 |
Coding |
TetX2 |
Konstantinos Afordakos |
1170 |
| BBa_K4056001 |
Coding |
ereB - Erythromycin esterase type II |
Konstantinos Afordakos |
1263 |
| BBa_K4056002 |
Coding |
His-Tag TetR |
Konstantinos Afordakos |
645 |
| BBa_K4056003 |
Coding |
HA-tag MphR |
Konstantinos Afordakos |
615 |
| BBa_K4056005 |
Regulatory |
Reverse pTet |
Konstantinos Afordakos |
54 |
| BBa_K4056007 |
Coding |
mf-Lon on the reverse strand |
Konstantinos Afordakos |
2367 |
| BBa_K4056008 |
Other |
12bp spacer with BamHI |
Konstantinos Afordakos |
12 |
| BBa_K4056013 |
RBS |
Reverse RBS with 6bp spacer |
Konstantinos Afordakos |
21 |
COMPOSITE PARTS
| Name |
Type |
Description |
Designer |
Length |
| BBa_K4056004 |
Composite |
TetX2 - EreB operon |
Konstantinos Afordakos |
2537 |
| BBa_K4056006 |
Composite |
Tetracyclines and Macrolides operated OR gate |
Konstantinos Afordakos |
176 |
| BBa_K4056009 |
Composite |
TetX2-EreB operon |
Konstantinos Afordakos |
2572 |
| BBa_K4056010 |
Composite |
AraC expression cassette |
Konstantinos Afordakos |
1055 |
| BBa_K4056011 |
Composite |
mf-Lon expression cassette |
Konstantinos Afordakos |
2546 |
| BBa_K4056012 |
Composite |
TetR-MphR bicistronic expression |
Konstantinos Afordakos |
1417 |
| BBa_K4056014 |
Composite |
Tetracycline and Macrolide control mf-Lon "OR" expression gate |
Konstantinos Afordakos |
5108 |
| BBa_K4056015 |
Composite |
pBad - bpDNase I with mf-Lon protein tag |
Konstantinos Afordakos |
1377 |
Throughout the project, we created a channel of communication with stakeholders. We consulted experts and held discussions with local businesses. During this contact, we established that our project focus is indeed important and that there is a necessity for a design that will be efficient, safe, and good for the people. We subsequently worked towards these goals.
We were also meticulous in our efforts to inform about the alarming AMR global problem, SDGs, the importance of One Health and the role of Synthetic Biology. To this end, we worked with multiple groups and people; we provided information on the current situation in our region, introduced the iGEM competition in our institution and presented our project as well as its potential socioeconomic impact.
Importantly, we collaborated with other iGEM teams from around the country and participated in a medical conference to spread knowledge on the issues. We also organized a scientific meeting and presented our work and ideas to a variety of fora. Notably, we worked on educating university and high school students and communicating our message to a broad audience.
