<!DOCTYPE html> Attributions

Attributions

Team Attributions

Attributions
		Akash	Arsh	Arya	Ashli	Ashwin	Jason	Likhith	Misaal	Namasi	Sanjana	Soorya	Vidisha
Human Practices	Integrated Human Practices
	Youtube Videos
	Social Media
	Newspaper Articles
	Activity Book
	Survey
	Disha Webinar
	SynBio Webinar
	Audio Webinar
	SynBio Talk - Prof. Pawan Dhar
	Cyanobacteria Symposium
	Comic Book
	Drama Club Silent Film
	Art and Essay Competition
	Attribution Table


Wet Lab	Constructs
	Parts
	Assay Design
	Protocols
	Wet Lab Logistics
	Cyanobacteria cell revival
	Cyanobacteria stock preparation
	Cyanobacteria Growth curve BG-11+ Sucrose sample collection
	Cyanobacteria Growth curve coBG-11+ Sucrose sample collection
	Cyanobacteria Sucrose Assay
	E coli stock preparation
	E coli cell revival
	E coli PCR + gel
	E coli gradient PCR + gel
	E coli plasmid extraction pcscX
	E coli competent cell preparation
	E coli cell transformation with pcscX
	MIC
	Growth curve + sucrose sample collection + butanol sample collection in LB
	Growth curve + sucrose sample collection + butanol sample collection in co BG11
	E coli sucrose assay


Dry Lab	Initial cyanobacteria model making
	Cyanobacteria FBA
	Cyanobacteria FSEOF
	Cyanobacteria OptKnock
	Cyanobacteria MoMA
	Cyanobacteria Strain Optimization for Toulouse
	E coli FBA
	E coli FSEOF
	E coli OptKnock
	E coli FVA
	SteadyCom for Toulouse
	Dynamic FBA
	SteadyCom


Collaborations	All India iGEM Meet
	Cyanobacteria Symposium
	GMO Survey
	Toulouse
	Guelph
	SZU China
	Linkoping



Administrative Work	Sponsorship
	Finance Management
	Ordering strains, parts and reagents
	Managing Discord
	Managing Notion
	Managing mails and calendar
	Meeting Notes
	Volunteer Handling


Wiki	Design
	Coding
	Documentation
	Home Page
	Editing Documentation

Safety

Deliverables	Promotion Video
	Presentation Video

Scientific Proposal

Design	Social Media Posts
	Posters
	Mascot Design
	Merchandise
	Presentation Slides
	Sponsorship Brochure

Mentors

Dr. Thomas Pucadyil - Our PI, without whom this project would not have been possible. He managed the administrative side of things and facilitated all our interactions with our institute to make sure our project progressed as smoothly as possible!

Prem Pritam - Prem was invaluable to the cyanobacterial front of our project. She guided us through every step of the cyanobacteria work, from setting up the lights in our incubator, supplying us with BG - 11 media, instructing us over phone calls about basic techniques to handle cyanobacteria (reviving, inoculating, plating, cryopreserving, etc.) to helping us design our growth curve assays, and our composite parts. She met with us regularly almost every week to get updates on our progress and guided us through the project. We are immensely grateful to her for all her time and effort, this project would not have been what it is without her!

Virmal Jain - Virmal too was invaluable to the cyanobacterial front of the project, helping us with the conceptualization of our experiments, designing assays and constructs, solving any queries we had, and helping us troubleshoot our experiments. He met with us regularly along with Prem, almost every week to get updates on our progress and guided us through the project. We are immensely grateful to him for all his time and effort, this project would not have been what it is without him!

Indumathi P - Indumathi mentored us through every aspect of our dry lab. She verified our methods and techniques used for our analysis. She was especially helpful whenever we ran into issues and showed us various approaches to troubleshooting. She also helped us reach out to her colleagues and mentors who worked closely on niche topics we needed help with. Indumathi has been crucial to our dry lab modeling and we are extremely thankful to her!

Rushik Bhatti - Rushik helped us plan out our E. coli growth assays, and guided us through genomic DNA extraction, plasmid purification, making electrocompetent cells, transformation, and cryopreserving our E. coli stocks. He helped us brainstorm project ideas, design experiments and plan a workflow for our experimental work, and helped troubleshoot our experiments. We are extremely grateful to him for all his help!

Devatrisha Purkayastha - Trisha familiarised us with using the biospectrometer for OD measurements, and helped troubleshoot PCR and the sucrose assay. She also helped us brainstorm project ideas, design experiments, and plan our experimental workflow. We are very thankful for all the help she provided us with!

Mukundan S - Mukundan was part of our early brainstorming sessions, giving us valuable feedback that helped us choose our project topic, and initially guided us with questions we had about modeling.

Mohit Mantri - Mohit helped us with autoclaving our glassware and preparing micronutrient solutions for our BG-11 growth medium, and was also a part of our early brainstorming sessions.

Chinmay Patwardhan - Chinmay helped us in the process of securing sponsorship and reaching out to our Ph.D. mentors, checked in with us from time to time to get updates on our project, and gave us advice on navigating the iGEM cycle and IISER administration.

Experts

Dr. Syed Shams Yazdani - Their suggestions allowed us to look at end products we could produce from the anaerobic pathway. They also offered us their butanol-producing E. coli KJK01 strain. Their inputs lead to one of the most significant modifications to our project, which was to change our final compound from succinate to butanol.

Dr. Himadri Pakrasi - Dr. Liberton is a research scientist at Prof. Himadri Pakrasi’s lab at Washington University in St. Louis. They facilitated the shipment of the Synechococcus elongatus UTEX 2973 strains to us and helped us with protocols and troubleshooting.

Dr. Nishad Matange - Dr. Matange provided us with help on the E.coli experimental design front and helped us with our sucrose assay troubleshooting. They very generously gave us some of their own lab’s glassware and antibiotics.

Dr. Richa Rikhy - Dr. Rikhy advised us to store our E.coli and S.elongatus supernatants at -20°C to prevent contamination before our sucrose assay.

Dr. Amrita Hazra - Dr. Hazra pointed out to us that engineering the aerobic pathway for succinate production in E. coli may quickly become limiting for the co-culture. This motivated us to consider picking a different end product for our co-culture.

Dr. Anand Ghosalkar - Dr. Ghosalkar gave us valuable insights into the butanol production process in E. coli, the downstream processing of butanol as well as the global butanol market.

Dr. Shireesh Srivatsava - Dr. Srivastava suggested we use Flux Balance Analysis, the core technique on which our entire modeling workflow is based. They also gave us insights into what parameters we should consider monitoring during the experimentation stage of our project.

Dr. Chhavi Mathur - Dr. Mathur gave us some very valuable inputs on our survey questions and told us about the concept of having a ‘survey story’ in order to make it more concise. Based on their insights, we redesigned the survey to better suit our goals of making it informative and engaging

Dr. Anamma Odaneth - Dr. Odaneth helped us gain insights into the butanol production pathway in E. coli and an analysis of the global butanol market

Dr. Anil Zankar - Dr. Zankar helped us design the script for the silent film that we were developing as an outreach initiative for students with hearing disabilities. Based on their inputs, we improved our script for accessibility and effective communication

Dr. Richard Lobo, Dr Ashok Dubey, Dr Malathy V - They gave us pointers on making the project more cost and resource-effective and helped us gain clarity on what our project would look like when implemented in a scaled-up real-world setting. Dr. Ashok Dubey and Dr. Malathy V also gave us extremely useful experimental inputs to help troubleshoot our experiments and make them more efficient.

Dr. Santanu Dasgupta - Dr. Dasgupta helped us in understanding the aspects of our project we should focus on to make our project a well-rounded model for sustainable production.

Dr. Shashi Kumar Rhode - Dr. Rhode brought to our attention rather pressing issues we should take into consideration while picking our chassis.

Dr. Drawak Ravikumar - Dr. Ravikumar introduced us to Life Cycle Assessments (LCA), which we plan to use to model the carbon footprint of our project. They also gave us a few frameworks that run LCA as open-source software.

Ms Manisha Kamal - After the meeting with Ms. Kamal, we learned how to design and curate a teaching session module for visually impaired students by stimulating their senses of touch, smell, and hearing.

Dr. Maziya Ibrahim - Dr. Ibrahim gave us their inputs on our GitHub page on SteadyCom -- ‘SteadyCom Checklist’, and their opinions on the methods we used for the co-culture analysis

Dr. Reena Pandit - Dr. Pandit helped us decide between various directions our project could take. They cautioned us against encapsulating the cyanobacteria, advised us to model the effect of oxygen evolved by the cyanobacteria on E. coli and provided inputs on our future implementation.

Wet Lab

Chinmaya Jena - Chinmaya helped us perform and analyze our MIC assays.

Soumya Swastik Sahoo - Soumya helped us with the NMR analysis of our culture supernatants.

Shravee Shetty (Shabnam) - They were in charge of the undergraduate lab at IISER Pune. They provided us with glassware and helped us understand how the lab instruments worked.

Kalpesh Thakare - Mr. Kalpesh works at the biostores of our institute. They handled the paperwork of all our orders and provided us with glassware, containers, stationery, etc. for our project.

Piyush Pradeep Gadekar - Mr. Piyush works at the biostores of our institute. They handled the paperwork of all our orders and provided us with glassware, containers, stationery, etc. for our project.

Hariprasad Dande and Mahesh - Mr. Hari and Mr. Mahesh helped us set up the lights for our incubator and managed all the other electrical work.

Outreach

Dr. Shanti Kalipatnapu - They helped us engage with the general public about our project through publishing articles in the media as a part of science communication.

Ms. Aparna Joshi - Theyhelped us circulate the survey among various high school students

Ms. Gayatri Kshirsagar - They helped us circulate the survey among various high school students.

Vaishnavi Pratap (@doodlesane) - They helped bring the story of Kalpana to life through their captivating illustrations in our comic book.

Soumyodeep Mukhopadhyay - They collaborated with us to record ‘How Would the World Be’, a song to raise awareness on climate change.

Ipsa Bezbarua - She helped write our comic series - Kalpana and the song - ‘How Would the World Be’.

Aarti Takawane - They helped us with reaching out to students at NBFM Jagriti school for Blind girls, in Vadgaon, for our audio webinar

Ms. Zainab - They helped us reach out to local NGOs in pune, for publicising our audio webinar

Dr. Pooja Sancheti - They provided us with valuable feedback on our survey for high school students and facilitated our meeting with the Doorstep School kids for an interactive session.

Dr. Aurnab Ghose - They went through our survey for high school students and validated that our survey was ethical and safe to circulate.

Disha - Disha is a student-run social outreach initiative at our institute, that aims for socioeconomic equity through education. They collaborated with us to organize an educational webinar for economically disadvantaged students and students belonging to marginalized communities.

Drama Club - The Drama Club at our institute collaborated with us to produce the silent film for students that are deaf or hard of hearing.

Science Club - The Science Club at our institute helped us organize the talk by Prof Pawan K Dhar on synthetic biology and its applications.

Doorstep School - They are an NGO that teaches underprivileged students that provided us with the opportunity to interact with their students for a session module on climate change based on our Activity Book.

Dr. Pushkar Sohoni - They graciously agreed to be a judge for Kalakriti - the art and essay competition we organized for school students.

Dr. Sudha Rajamani - They graciously agreed to be a judge for Kalakriti - the art and essay competition we organized for school students.

Dr. Suhita Nadkarni - They graciously agreed to be a judge for Kalakriti - the art and essay competition we organized for school students.

Dr. Kalika Prasad - They graciously agreed to be a judge for Kalakriti - the art and essay competition we organized for school students.

Collaborations

Team INSA-UPS - They collaborated with us in hosting the Cyanobacteria Symposium and carried out a 5-month long Partnership with our Dry Lab team.

Team StonyBrook - They collaborated with us in hosting the Cyanobacteria Symposium and the Synthetic Biology webinar for high school students.

Team Hong Kong HKU - They collaborated with us in hosting the Cyanobacteria Symposium and the Synthetic Biology webinar for high school students.

Team MSU Maastricht - They collaborated with us to design the international GMO survey.

Team IISER Tirupati - They collaborated with us to design the international GMO survey and All India iGEM Meet.

Team Aachen - They collaborated with us to design the international GMO survey.

Team Guelph - They collaborated with us to create educational content for students and teachers in Canada.

Team SZU China - They collaborated with us to translate and circulate the educational content created by their team.

Team Linkoping - They helped us distribute our high school survey and we gave them inputs on mathematical modeling.

Team IISER Berhampur - They collaborated with us as a part of the organizing committee of the All India iGEM Meet.

Team IISER Thiruvananthapuram - They collaborated with us as a part of the organizing committee of the All India iGEM Meet.

Team IISER Bhopal - They collaborated with us as a part of the organizing committee of the All India iGEM Meet.

Team IISER Kolkata - They collaborated with us as a part of the organizing committee of the All India iGEM Meet.

Team MIT_MAHE - They collaborated with us as a part of the organizing committee of the All India iGEM Meet.

Team IISc - They collaborated with us as a part of the organizing committee of the All India iGEM Meet.

Special Thanks

Prof. Pramod Wangikar - Due to the devastating second wave of the COVID-19 pandemic in India, we were severely pressed for time in the lab. Prof Wangikar generously offered to provide us with cyanobacteria strains of wildtype S. elongatus UTEX 2973 and parts that we required for our experiments. They also introduced us to his Ph.D. students Prem Pritam and Virmal Jain who graciously agreed to mentor our wet lab team.

Dr. Suhita Nadakarni - In-person meetings had become significantly difficult during the pandemic. Our team wanted to host an interactive session with a few underprivileged kids and getting them on campus for a meeting would have been a logistical nightmare if it wasn’t for Dr. Suhita Nadakarni who very kindly offered to host this meet-up at her house!

Somen Phanse - Somen went out of his way to take multiple trips to the printing house to get our activity books printed for us. He managed all the logistics very efficiently and got our team yummy modaks during the Ganpati festival!

Varsha Jaisimha - Varsha gave us valuable feedback and inputs on navigating the iGEM competition.

Lakshmi Sriram - Lakshmi helped us edit some of the wet lab documentation write-ups for our wiki.

Abhinav Masih - Abhinav helped us with lab techniques, protocols, and wet lab troubleshooting.

Sponsors - Our project could not be made possible without the generosity of our sponsors, Sai Life Sciences, the Family Office of Mr. Kris Gopalakrishnan, ZOHO, Notion, Geneious, and IDT.

Dr. Srabanti Chaudhury - Dr. Chaudhury is a member of the Task Force Committee at our institute. THey helped us with all the paperwork while we were in the process of coming back to campus.

Prabhas Patankar - Mr. Prabhas is the accountant at our institute. They processed all our orders and reimbursements.

Survey Translator - We asked our friends from different parts of the country to translate the high school survey into various regional languages to reach as many people as possible. We are very grateful for all their help!

Translator	Language
Prathith Bhargav	Kannada
Vasudha Kulkarni	Kannada
Muhsina H	Bengali
Ningnung Jakoinao	Punjabi
Moshikeeran	Tamil
Pranav Maheshwari	Hindi
Vikram Iyer	Hindi

Vikram Venugopal - He helped procure information about biobutanol statistics and the global market.

Volunteers

Design Volunteers - Abhilipsa Das, Devapriyan, Sneha Jain, Yatish Juneja, Ningnung Jakoinao

Abhilipsa, Devapriyan and Sneha were extremely helpful in helping us present our project in an engaging and effective manner to the general public through design and illustration. They designed and redesigned posters for social media and played a huge role in the success of our activity book. Their sincere work made our wiki what it is, with all its illustrations and design elements. They were incredibly creative, helpful, earnest, and hardworking. We are extremely grateful to them for sticking with us throughout the competition despite all their coursework and other activities that they partook in. We would like to thank Ningnung for his help during the initial stages of our project. We would also like to thank Yatish for his help in designing our posters for the High School Survey as well as the Synbio talk in collaboration with the Science Club.

Sponsorship Volunteers - Oyindrila Samantha, Akriti Parashar, Manas Raikar, Hrishikesh Jagtap

The sponsorship volunteers helped us by researching and contacting potential sponsors for our project. They tirelessly drafted emails and made several calls to pitch our idea to potential sponsors.

Outreach Volunteers - Vikram Venugopal, Anish Mulchandani, Akriti Parashar, Oyindrila Samantha, Sumant Chopde, Shreya Kulkarni, Abhishek Vaidya, Ipsa Bezbarua

The outreach volunteers helped us by publicizing all our public engagement and education initiatives. They took care of the logistics behind the art and essay competition by sorting and running plagiarism checks on each and every entry and helped us procure contacts and organize the audio webinar. They also helped us in curating our educational material in the form of activity books and comics. Our song on climate change and the comic series - Kalpana were written in collaboration with our volunteers. We are very grateful for their enthusiasm and dedication to our project who have constantly shown their support and have believed in our idea.

Social Media Volunteers - Krish Pandey, Krishna Menon, Aditi Krishnan, Vihang Vaidya, Anuj Chaturvedi

Social media played an indispensable part in our iGEM journey. It helped us reach a wider audience, connect and collaborate with other iGEM teams, and find sponsors. The volunteers were enthusiastic and helped us collect information and design social media posts. They actively reached out and collected the contact information of potential sponsors.

The volunteers were especially helpful in keeping track of interactions with other teams and sponsorship challenges on Twitter and their respective deadlines.

Apart from serious work, it was enjoyable to create infographics and social media ‘trends’ with inputs from the volunteers.

Wiki - Pritam Acharya, Jatin Bedi, Purva Pamar

Pritam helped us in styling parts of the wiki and setting up the Team page for us. Jatin and Purva, being members of the IISER-Pune-India 2020 wiki team, helped us tremendously in helping us get started and ran us through important things to keep in mind. Our wiki even makes use of some of the elements from their wiki, and their help was indispensable in making our wiki what it is.

Team:IISER-Pune-India/Attributions