Collaborations

1.OUC-R donated us the plasmid and troubleshoot the transformation for our project:

https://2021.igem.org/Team:OUC-R/Collaborations#ICJFLS_Team

In our project, three genes (ADG1, APL1 and GBSS1) are required to transfer into the E. coli for synthesis amylose. Two of them (ADG1 and APL1) are contained to subunits of the ADP glucose pyrophosphorylase (AGPase). At first, we cloned 3 genes into 3 vectors, then tried to do co-transformation. However, we failed. After several failure attempt, we consulted the OUC-R, which is a university team. They suggested that we can use one plasmid pETDuet-1 to deal with that problem because this vector can be inserted two genes at the same time. It is a bi-expression vector containing two promoters. And they donated this vector to us. So, we inserted ADG1 and APL1 into plasmid pETDuet-1. This plasmid is crucial for us to clone genes. Our problem was simplified. We just need to transfer two plasmids for synthesis amylose. It is easier than the transformation of 3 plasmids. It is really a help! We appreciate them very much!

2.We donated the Nissle 1917 to NJTech_China to help them complete their experiment:

https://2021.igem.org/Team:NJTech_China/Collaborations

In the iGEM China Community, NJTech_China released a news of seeking help. The needed Nissle 1917 for completion some experiments. There is this strain in our laboratory just in time. So, we connected them and donated this strain to them. Our donation is really helpful for them to complete their project. We are very happy to help them. Our related communication records are showed as follows:

3.Collaboration with NMU_China in Education and Communication:

https://2021.igem.org/Team:NMU_China/Collaborations

We had collaboration with NMU_China in Education and Communication. They prepared the education materials, and we use their materials to give a lecture for the junior students in Jinan Foreign Language School. Our topic of this Support Education activity is What is Covid19 and How to treat Covid 19 by using bio-synthesis technology. In addition, Fengjun Sui summarized and popularized the concept of synthetic biology. He compared molecular biology with Lego building blocks in figurative rhetoric, and stated the characteristics and key words of this emerging field of knowledge, so as to make a clear distinction from traditional biology. During the whole activity process, all the students listened very carefully. Many of them took notes and actively cooperated in asking questions. This teaching support education activity ended with Xingshen Wang's summary. He mentioned that he hoped interested students could continue to consult relevant materials after class and become the new iGEMer in the future if possible then contribute to the development of biology.

4.XJTU-China troubleshoot the SDS-PAGE electrophoresis for our project:

https://2021.igem.org/Team:XJTU-China/Collaborations

Some enzyme genes are cloned into the expression vectors pETDuet-1 and pET-28a (+) in our project. In order to test the expression of these enzymes, we did the SDS-PAGE electrophoresis. It is more difficult than agarose electrophoresis. And it is the first time for our senior high school students. We encountered some problems. After many times failure, we consulted a university team XJTU-China. With their help, we modified some processes and conditions of SDS-PAGE. Finally, we did it successfully. We want to express our many thanks for their assistance.

5. Participate in the 8^th CCIC:

We participated in the 8th conference of China iGEMer community meeting held in Shanghai, China from August 27 to 29, 2021. The theme is to overcome challenges and constantly surpass. The content of the meeting was to share IGEM team organization and management experience and bring guidance and inspiration to the participating teams. Solve the doubts and problems of team organization and management. Discuss excellent strategies and methods of organizational management.