Team:EPFL/Parts Collection

• Project
  • Description
    Project background, inspiration, etc.
  • Background
    Quantification of the problem
  • Design
    Monomer and dimer design
  • Modelling
    Bioreactor fluid mechanics models
  • Results
    Main results of the project
  • Engineering Success
    Design, build, test, learn
  • Implementation
    Bioreactor
  • Proof of Concept
    Showing yeast captures copper
  • Contribution
    Contribution to future teams
  • Safety
    Chemicals used, security measures
  • Protocols
    PCR, Western Blot, transformation, etc.
  • Notebook
    Detailed records of all experiments
• People
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• Parts
  • Parts Overview
  • Parts Collection
• Human Practices
  • Human Practices
  • Integrated Human Practices
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Parts Collection

Contents

• Introduction
• List of parts
  • Basic parts
  • Composite parts

Introduction

We have made different constructs to modify the yeast EBY100 to make it express the copper metallothionein CUP1 protein at its external surface. Here is the complete list of all the parts we have created to transform the yeast. As basic parts, we have the CUP1 alone fused with Aga2 and V5 as well as seven dimers. We also have 8 composite parts that are their corresponding expression systems, namely Gal1 – Aga2 – protein – V5.

Our favorite basic parts are BBa_K4035001 and BBa_K4035007 and our favorite composite parts are their corresponding expression system BBa_K4035000 and BBa_K4035014.

List of parts

Basic parts

Name	Type	Description	Assembly compatibility
BBa_K4035001	Coding	CUP1 fused to Aga2 and tagged with a V5 epitope	Type IISRFC[10]
BBa_K4035002	Coding	Dimerization of the copper metallothionein 1: CUP1–(GGGGS)3–CUP1	Type IISRFC[10]
BBa_K4035003	Coding	Dimerization of the copper metallothionein 1: CUP1–(GGGGS)4–CUP1	Type IISRFC[10]
BBa_K4035004	Coding	Dimerization of the copper metallothionein 1: CUP1–(AP)7–CUP1	Type IISRFC[10]
BBa_K4035005	Coding	Dimerization of the copper metallothionein 1: CUP1–(EAAAK)3–CUP1	Type IISRFC[10]
BBa_K4035006	Coding	Dimerization of the copper metallothionein 1: CUP1–(EAAAK)4–CUP1	Type IISRFC[10]
BBa_K4035007	Coding	Dimerization of the copper metallothionein 1: CUP1–GGGGSEAAAKGGGGS–CUP1	Type IISRFC[10]
BBa_K4035008	Coding	Dimerization of the copper metallothionein 1: CUP1–GGGGS(EAAAK)2GGGGS–CUP1	Type IISRFC[10]

Composite parts

Name	Type	Description	Assembly compatibility
BBa_K4035000	Composite	Expression of CUP1 at the surface of EBY100	Type IISRFC[10]
BBa_K4035010	Composite	Expression of the CUP1–(GGGGS)3–CUP1 dimer on the extracellular membrane of S. cerevisiae	Type IISRFC[10]
BBa_K4035011	Composite	Expression of the CUP1–(GGGGS)4–CUP1 dimer on the extracellular membrane of S. cerevisiae	Type IISRFC[10]
BBa_K4035012	Composite	Expression of the CUP1–(AP)7–CUP1 dimer on the extracellular membrane of S. cerevisiae	Type IISRFC[10]
BBa_K4035013	Composite	Expression of the CUP1–(EAAAK)3–CUP1 dimer on the extracellular membrane of S. cerevisiae	Type IISRFC[10]
BBa_K4035014	Composite	Expression of the CUP1–(EAAAK)4–CUP1 dimer on the extracellular membrane of S. cerevisiae	Type IISRFC[10]
BBa_K4035015	Composite	Expression of the CUP1–GGGGSEAAAKGGGGS–CUP1 dimer on the extracellular membrane of S. cerevisiae	Type IISRFC[10]
BBa_K4035016	Composite	Expression of the CUP1–GGGGS(EAAAK)2GGGGS–CUP1 dimer on the extracellular membrane of S. cerevisiae	Type IISRFC[10]

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