Team:EPFL/Contribution

• Project
  • Description
    Project background, inspiration, etc.
  • Background
    Quantification of the problem
  • Design
    Monomer and dimer design
  • Modelling
    Bioreactor fluid mechanics models
  • Results
    Main results of the project
  • Engineering Success
    Design, build, test, learn
  • Implementation
    Bioreactor
  • Proof of Concept
    Showing yeast captures copper
  • Contribution
    Contribution to future teams
  • Safety
    Chemicals used, security measures
  • Protocols
    PCR, Western Blot, transformation, etc.
  • Notebook
    Detailed records of all experiments
• People
  • Team
  • Attributions
  • Collaborations
• Parts
  • Parts Overview
  • Parts Collection
• Human Practices
  • Human Practices
  • Integrated Human Practices
  • Education & Communication
• Medals

Contribution

Contents

• • Introduction
  • Linkers for fusion proteins
  • A guide for using copper concentrations using cuprizone
  • A Raspberry Pi code
  • A protocol for making beads
  • A 3D model for 3D printing a fluorimeter

Introduction

Our main contribution for future iGEM teams was the design of protein linkers. In addition to that, we have listed below other elements that we think could be useful to anyone interested.

Linkers for fusion proteins

Fusion protein design typically requires linkers design. The choice of the whole complex is based on the desired functions of the fusion protein product and, in most cases, is relatively straightforward. On the other hand, the selection or rational design of a suitable linker to join the protein domains together can be arduous and is often the most critical step in designing a dimer. During these months we have put our heads together to design and test seven different linkers and we are finally pleased to share our results with the iGEM community.

Click here to find this guide.

A guide for using copper concentrations using cuprizone

Click here to find this guide.

A Raspberry Pi code

Future iGEM teams most probably needing to use a raspberry pi for driving a pressure sensor using I²C, for making a GUI, or reusing our GUI even, for making a bioreactor, we offer the community a Git repository with all of our code. The README file gives all the information needed about the packages used, where to find them and how to install them. A more detailed account of what was done can be found on our Hardware page and GitHub repository.

A protocol for making beads

This protocol is based on team Eindhoven’s 2015 protocol, but it has been adapted to be used in higher throughput applications where beads are a means of water treatment.

A 3D model for 3D printing a fluorimeter

The fluorimeter itself was designed by iGEM team of Moscow, but the model we put together for them is a contribution of our own which we believe could help future teams looking for a feasible low cost piece of equipment. For additional details, see the Collaborations page.

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