1 Leaders
Sizhe Chen
1. Designed the project and participated in most of wet experiments.
2. Established the procedures of dry experiments.
3. Led the whole team to finish every necessary target.
Yuliang Huo
1. Gathered team members and organized the team.
2. Organized weekly meetups.
3. Offered the imagine of our project.
4. Designed the process of the experiment.
5. Connected with other teams.
6. Wrote and proofread our experimental process.
7. Wrote and proofread the third part of HP.
8. Arranged works.
2 Experiments
Jing Chen
1. Contacted with other teams to cooperate and communicate.
2. Participated in the wet experiments.
3. Participated in writing for some aspects.
Yan Chen
1. Participated in background research at the beginning of the project.
2. Participated in writing and rewriting for some aspects.
Qi Cui
1. Participated in the wet experiment group and the HP group.
2. Participated in the writing of wet experiment story and related design of parts.
3. Publicized the harm of microplastic to the community residents by visiting the community and interviewing
the masses.
4. Participated in the writing of the HP group copywriting and related contents of project on the team
official account.
Peihang Gu
1. Participated in the writing of wet experiment story and related design of parts.
2. Participated in wet experiments and mainly completed part of gene-related work.
Lingjie Hou
1. Participated in wet experiments and mainly completed part of protein-related work:
(1) Activation, induced expression and fragmentation of recombinant bacteria;
(2) Collection, isolation and purification of proteins;
(3) Identification of successful expression of enzymes and determination of enzyme activity, etc.
2. Participated in the early HP, including the following:
(1) Preliminary background investigation;
(2) Safety Form writing;
(3) Schedule writing, etc.
Yanming Jin
1. Assisted and organized experiments.
2. Participated in background research at the beginning of the project.
3. In charge of the human practices group,provided ideas and attended meetings.
4. Communicated with other iGEM teams.
Yi Li
1. Wrote the Human Practice process.
2. Contacted with other teams to cooperate and communicate.
3. Conducted the early wet experiments.
4. Participated in group meetings and discussed opinions.
Zongqi Li
1. Participated in group meetings and discussed opinions.
2. Participated in the preliminary design experiment process.
3. Did the wet experiments.
4. Participated in HP Education part.
Yuanyuan Liu
1. Held virtual meetups.
2. Helped designing the project.
3. Took charge of the molecular biology part of wet lab experiments.
Songlin Ma
1. Found some literature, summed up the harm of plastics to people, as well as the current methods of
degradation of plastics.
2. Did some auxiliary experiments.
Shupeng Piao
1. Project preparation: Organized and assigned background research team work, provided ideas and organized
meetings.
2. Participated in connection, transformation and colony PCR.
3. Participated in protein extraction and activity detection.
4. Human practice: Interviewed experts and stakeholders, and recorded the interview content.
5. Safety part in wet experiments and flowchart part in integrated human practice writing.
6. Edited iGEM related push on WeChat platform and slides.
Binghui Song
1. Led a group to conduct extensive wet experiments, such as constructing engineered bacteria.
2. Discussed with the team leader how to conduct the experiment.
Gerui Yin
1. Participated in experiments.
2. Participated in weekly group meetings.
3. Helped to write articles about HP.
Liting Yue
1. Wet lab ideals construction.
2. Experimental protocol assistance.
3. Experimental schemes design.
Xuanshuo Zhang
1. Participated in the team management, improved the division of labor, plotted Gantt chart.
2. Participated in experimental operation.
3. Researched the background of plastic pollution.
4. Designed the questionnaire of HP.
5. Raised a bit funds.
6. Wrote a business plan.
Yihan Zhang
1. Wrote the Human Practice process.
2. Contacted with other teams to cooperate and communicate.
3. Conduct the early wet experiment.
Haiyue Zhou
Did the experiments of constructing plasmid vector.
Wenjing Zhou
1. Participated in wet experiments and completed part of protein-related work:
(1) Identification of successful expression of enzymes;
(2) Purification of expressed protein and multiple characterizations of proteins.
2. Participated in the early HP, including the following:
(1) Preliminary background investigation, including collecting data and consulting literature;
(2) attended team collaboration meetings to build stable partnership;
(3) Participated in team promotion (official accounts).
3 Human Practices
Jingxuan Qi
1. Wrote the Human Practice process.
2. Contacted with other teams to cooperate and communicate.
3. Conducted the early wet experiments.
4. Participated in human practice and connected with the community.
Jie Ren
1. Participated in the HP group.
2. Publicized the harm of microplastic to the community residents by visiting the community and interviewing
the masses.
3. Participated in the writing of the HP group copywriting and related contents of project on the team
official account.
Wenbo Chen
1. Took group photos and personal photos of members, as well as experimental photos.
2. Participated in the HP group.
3. Publicized the harm of microplastic to the community residents by visiting the community and interviewing
the masses.
4. Participated in the writing of the HP group copywriting and related contents of project on the team
official account.
4 Video and Art Design
Anqi Ren
1. Designed the team logo.
2. Drew the elements for the science game which is a part of Human Practices.
3. Drew the background images of the deliverables throughout the competition.
4. Designed the team flag.
5. Drew the vector elements required for the project promotion video.
6. Drew the flow chart of Human Practices.
7. Drew the layout of the wiki pages.
Limin Zhang
1. Drew the background images of the deliverables throughout the competition.
2. Designed the team flag.
3. Drew the vector elements required for the project promotion video.
4. Drew the flow chart of Human Practices.
5. Drew the layout of the wiki pages.
Yiheng Zhao
1. Project promotion video subtitle, music, script and animation.
2. Edited the cooperation promotion video (with NNU-China).
3. Participated in background research of the project.
4. Participated primer design at the beginning of the project.
5 Wiki Construction
Taosong Fang
1. Wrote the code of the wiki pages
2. Designed the animation on the home page.
3. Used machine learning to model and analyze the information of HP questionnaire.
4. Wrote the code of some part pages.
5. Participated in communication activities with other teams.
Enze Zhang
1. Wrote the code of the wiki pages except the home page.
2. Collected most of the source materials from other groups.
3. Designed simple styles for our wiki.
4. Wrote the code of some part pages.
5. Provided suggestions about fixing the problems about wiki construction for team Tianjin,
CPU_CHINA and
GreatBay_SZ.
6 Instructors
Jun Yang
1. Provided guidance to Human Practices.
2. Promoted the progresses of the project.
3. Pointed out the main contradictions at the critical stage of the experiment.
4. Suggestions on the practicality of the project.
5. Provided us with the latest research progress on related projects.
6. Provided financial support.
Lihan Zi
1. Organized competition training.
2. Promoted the progresses of the project.
3. Provided technical support and guidance.
4. Applied and organized team activity funds.
Zhanyou Chi
1. Provided facility and supplies for lab work.
2. Advices on research strategies.
3. Provided latest progress on microalgae related research.
Fantao Kong
1. Promoted the progresses of the project.
2. Pointed out the main contradictions at the critical stage of the experiment.
3. Provided us with the latest research progress on related projects.
Li Xu
1. Provided lab and equipments.
2. Organized the meetings between students and advisors.
3. Provided some advice on how to design experiments.
4. Provided suggestions on experiment methods.
7 Advisors
Wei Kang
Provided professional knowledge of protein engineering method and inspired us to apply his discovered
RIDD-RIAD peptides into our project.
Bolong Li
1. Offered suggestions to the student leaders on daily affair.
2. Provided critical discussions on dry experiment.
Shuchang Wang
Provided us experimental equipments and helped the team finished the experiments.
Guoxin Yang
Provided professional guidance in protein based experiments.
8 Acknowledgements
Gratitude to the professor and teacher.
We thank these people for their selfless help and guidance.
Professor Zhiwei Zhu provides corresponding ideas for plasmid designing.
Associate Professor Wei Kang inspired us of the idea of RIAD-RIDD system.
Graduate Guoxing Yang leads us to do protein related experiments.
Doctor Shuchang Wang helped us establish TPA characterization method and provided necessary samples and HPLC
for further experiments.
Teacher Yanbin Feng helped us to make our complex more applicable and effective.
Professor Yu Fan helped us understand the purpose and reality of the project more.
Acknowledgement to the support of school.
Thanks to the International Office, Academic Affairs Office, School of Bioengineering, School of Innovation
and Entrepreneurship of Dalian University of Technology for their support.
Thanks to the sponsors providing us economically.
At the same time, thanks to the Foundation and GenSci for their economic support. Thanks to all people,
institutions and enterprises that once assisted us!
Ailurus vecTM can provide the best expression vector for a specific protein in certain throughput.
With the sponsorship of Ailurus vecTM, we tried the throughput of N = 1,536 to optimize
the
output of MHETase in our project.
