Team:DKU/team

Team Introduction

                                                          Duke Kunshan University DKU iGEM Team 2021

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Group photo of bio team, from left to right: first row, Yunji Lee, Yixuan Wu; second row, Jiani Chen, Yunfei Lyu, Ruoxian Xu, Zhenyu Xu, Yang Yu; third row, Ziqin Wang, Jingsong Shan.



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Group photo of tech team, from left to right: Yufan Zhang, Zichao Chen, Jingwei Li, Yuxuan Wang

Attributions

iGEM is an interdisciplinary competition across biology, math, and social science. People with different knowledge should work together to achieve their goals. We appreciate the help we received in various fields. We could not be more grateful to the people who believed and supported us.

Team members

Jiani Chen is the team leader. She built up the team and was responsible for the overall progress of our team. She was a good communicator who helped to reach out to different experts in various fields. Jiani also represented the team to contact Duke and iGEM teams in Beijing and Suzhou for collaboration.

Jiani Chen is the team leader and initiator of DKU iGEM Team. She is a junior from DKU and majoring in cell and molecular biology. She was the mentor for high school iGEM teams in 2020. This year, she gathers funds from all divisions in the school and companies and build up a team with both sophomores and junior, majoring in STEM in DKU. As the project starts, she takes care of the algae culturing and protein expression in wet lab. She contacts biology, human practice, math modeling resources for the team. She also initiated the partnership between DKU and Duke iGEM team.

Yang Yu is the wet lab lead. He was responsible for the design and oversaw the progress of the experimental part of our project. He was also responsible for ordering lab apparatus and solutions as well as creating protocols. Yang also conducted the training for new wet lab members.

Yang Yu comes from Qingdao, China. He is majoring in molecular bioscience at Duke Kunshan University. He works as the technical director that is responsible for providing technical support, purchasing reagents, and making effective plans to keep the lab work on track. In the wet lab, he focuses on protein expression, identification, and purification. He also participates in human practice including interviewing professionals in the area and introducing the DKU iGEM project.

Ziqin Wang is the wet lab lead. He was responsible for the expression of the algicidal polypeptide. He designed the DNA sequence and made E. coli express it. Ziqin also contributed to the human practice part by interviewing the BlueGreen water technologies. He was the presenter at the monthly meet-up with DKU Innovation and Entrepreneurship department.

Ziqin Wang, from Chengdu, China, is now a junior at Duke Kunshan University. He majors in Molecular Bioscience: Cell & and Molecular Biology. He works as the lab manager responsible for the design and experiments related to peptide synthesis in the DKU IGEM team. He also participates in the design of the questionnaire and manages the interview with BlueGreen Water Technology Ltd. (China), a professional company providing mature solutions to water bloom. He is one of the main presenters to introduce the project that the DKU IGEM team is working on to professors, students, and interested stakeholders.

Ruoxian Xu is the treasurer. She was responsible for the quarterly budget plan and application for reimbursement. She also worked in the lab to help Ziqin with the polypeptide expression. Ruoxian contributed to human practice part by taking part in several interviews.

Jingsong Shan is the modeling lead. He was responsible for the math modeling part and created a 3D model of our floating unit. He did work on exosomes in the wet lab and was responsible to make E. coli produce exosomes as nanoparticles of the polypeptide.

Jingsong Shan comes from Nanjing, China. He is majoring in molecular bioscience at Duke Kunshan University. In the DKU IGEM team, he takes responsibility for the design and experiments in the exosome part, including technology like CRISPR, ultrafiltration, flow cytometer, etc. In the dry lab, he focuses on the building of the polypeptide diffusion model, and also participates in the design of the floating unit and making the 3D model. For the human practice part, his work includes interviewing professionals in the area and introducing the DKU IGEM project.

Yunji Lee is the human practice lead. She created a concrete plan for the human practices and collaboration parts of our project. Yunji also worked together with Jingsong on math modeling and exosome.

Yunfei Lyu worked in the lab and was responsible for the algae culture and test on the algicidal effect of the polypeptide. She was responsible for charting the algae growth curve and identifying the algal species in the water sample we brought from Taihu Lake.

Yunfei Lyu (Daisy) is from Shanghai, China. She is a junior of DKU major in Molecular Bioscience: Genetics and Genomics. In this iGEM team. Her main duty is algae culture including single colony separation from Taihu water sample, algae growth curve measurement, algicidal effect testing, and building algae growth model. In human practice part, she participated in the interview with Prof. Yajuan Lin from DKU, and contacted and interviewed Prof. Min Zhang from Nanjing Institute of Geography & Limnology. She also designed, distributed, and analyzed questionnaires with teammate Ziqin to see the public’s attitudes towards algal bloom. She hopes that this project can make a positive influence on algal bloom controlment.

Zhenyu Xu helped Yunfei with lab works on algae culture. She worked with Yixuan on the promotion video and designing posters for different events. She and Yixuan contributed a lot to the literature review and educational part.

Zhenyu Xu comes from Suzhou, China. She is currently a sophomore in Duke Kunshan University and plans to major in Material Science (Physics Track). She plays the piano and violin, and enjoys watching anime. She joins in DKU iGEM team looking for chances to dive in a scientific research. In the team, she is mainly involved in computer modeling and exosome experiment group. At the same time, she also participats in the poster and video production of Human Practice part.

Yixuan Wu worked with Zhenyu on the promotion video and designing posters for different events. She and Zhenyu contributed a lot to the literature review and educational part. Yixuan also helped to promote iGEM Club by joining the DKU club expo and DKU medi-podcast.

Zichao Chen, Jingwei Li, Yuxuan Wang and Yufan Zhang were students who worked together on DKU iGEM mathematical modeling process and the construction of whole wiki web page. What's more, they also designed iGEM team logos together.

Zichao Chen is a Data Science major student at DKU, he led the formation and working of DKU iGEM's tech group. Jingwei Li and Yuxuan Wang are also Data Science major student at DKU, they have played excellent role in wiki web page construction and mathematical modeling. Yufan Zhang is also a Data Science major student at DKU, he did a great job in the art design of logo and wiki web page.



Supervisor

Linfeng Huang, Associate professor of biology at Duke Kunshan University. He guided us throughout the project and provided us with precious suggestions and experiences.



Advisor

Shixin Xu, Assistant professor for mathematics at Duke Kunshan University. He provided great help in math modeling.

Davy, our iGEM official mentor. He gave us valuable advice on how to carry out human practice and math modeling.



Lab support

Yingxue Li, Suhua Li were biology lab manager in DKU. They secured our access to the lab.



Human practice

Hans W. Paerl, Kenan Professor of Marine and Environmental Sciences, at the UNC-Chapel Hill Institute of Marine Sciences, Morehead City. He accepted our interview, shared his research, and gave advice on our project.

Chuanhui Gu, Associate professor of environmental science at Duke Kunshan University. He accepted our interview, shared his research, and gave advice on our project.

Huansheng Cao, Assistant professor of environmental science at Duke Kunshan University. He accepted our interview, shared his research, and gave advice on our project.

Yajuan Lin, Assistant professor of biogeochemistry at Duke Kunshan University. She accepted our interview, shared her research, and gave advice on our project.

Min Zhang, Ph.D. and associate Research Fellow of Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences. He accepted our interview, shared his research, and gave advice on our project.

Pu Wang, China director of operations of BlueGreen Water Technologies. She accepted our interview, shared the company's methods of operation and product design and gave advice on our project.

DKU MediHealth Podcast, worked with our iGEM team to create a podcast to promote and introduce the iGEM project.



Sponsor

DKU Innovation and Entrepreneurship department had been very supportive of our project and has given most of the financial support.

DKU Student Affairs had been very supportive of our project and has given most of the financial support.

Nikon provided us with their generous financial support.

Marcia France, Dean of undergraduate studies at Duke Kunshan University. She provided us with their generous financial support.

Kai Huang, Division chair of natural and applied sciences of undergraduate program at Duke Kunshan University. He provided us with their generous financial support.



Partnership

iGEM team of DKU and Duke had a close relationship and helped each other through the whole process.