Team:Bolivia/Contribution
We added 3 new basic parts and 4 composite parts to the registry with information of its sequences and some details from the literature. These parts come from key modules of our biosensor constructs such as the transcriptional amplifiers, intracellular arsR control systems and the violet reporter. In addition, we have elaborated a couple of manuals with information that we believe will be very helpful for teams that, for example, want to carry out cloning experiments for the first time or build a device that detects color changes in paper strips.
Parts overview
| Part’s link | Type | Part’s name | Short description |
|---|---|---|---|
| BBa_K4007000 | Basic | mRFP1-Violet | Coding sequence of a violet mRFP1 variant. |
| BBa_K4007001 | Basic | pHrpL (σ54 optimized) | Sequence of inducible promoter pHrpL optimized by Wang et al. adopting the consensus σ54 promoter sequence for increased output expression. |
| BBa_K4007002 | Basic | RinA_p80α protein | Coding sequence of a proteín that acts as activator for the transcription in an ultrasensitive way. |
| BBa_K4007004 | Composite | Ultrasensitive biosensing complex por arsenic (#1) | Genetic construct with a double amplifying cascade that offers sub-5 ppb [As] sensitivities with an coloured output. |
| BBa_K4007005 | Composite | Ultrasensitive biosensing complex por arsenic (#2) | Genetic construct with a simple amplifying system that offers sub-10 ppb [As] sensitivities with an coloured output. |
| BBa_K4007007 | Composite | Intracellular arsR density control system (medium density) | Genetic construct with a medium strength constitutive promoter controlling the arsR gene expression. |
| BBa_K4007008 | Composite | Intracellular arsR density control system (high density) | Genetic construct with a high strength constitutive promoter controlling the arsR gene expression. |
Synthetic biology laboratory manual:
At the beginning of the competition we found difficulties with the accessibility of reliable information in spanish. For this reason we considered research, compile, translate and materialize several of the most common techniques in synthetic biology into a comprehensive and easy to understand laboratory manual. During its development, it helped several of us understand many of the basic steps for the experiments we are planning to carry out, and we hope it will also be of great help to other Spanish-speaking teams who have encountered similar difficulties.
Device manual:
We elaborated a very detailed manual for our device which includes instructions for assembly, correct use, 3D models, exact dimensions for its parts and some downloadable files for 3D printing. We hope it will also be of great help to other future projects with similar hardware in mind.
