Team:Open Science Global/Parts

Friendzymes
Part Collection
Results
Wetware Parts Parts Collection Notebook Safety
Part Collection
Overview Basic Parts Composite Parts

Overview

The main goal of our project is to make a frugal biofoundry capable of producing high quantities of enzymes and other proteins of interest. To do that, our team designed a library of parts in order to test in a high-throughput way the combinations that would generate the best results. For this year, the most important part generated by our team was the pHT43 plasmid (BBa_K4103000), an improvement on (BBa_K1074001) from 2013 - this plasmid acts as a shuttle vector between E. coli and B. subtilis, and allows the efficient production and secretion of our protein-of-interest, with our team focusing efforts to use this vector for the production of Pfu-Sso7d Polymerase (BBa_K4103011) codon optimized for B. Subtilis using the software created by our team.

For how we made our parts, see "Friendzymes BioBlaze Notebook".

Basic Parts

Part Name Type Description Length (bp)
http://parts.igem.org/Part:BBa_K4103000 pfu
http://parts.igem.org/Part:BBa_K4103001 Conjugation origin Transfer Origin pIP501 319
http://parts.igem.org/Part:BBa_K4103003 Resistance marker SpecR 989
http://parts.igem.org/Part:BBa_K4103004 Origin of replication EcoORI 589
http://parts.igem.org/Part:BBa_K4103005 Origin of transfer OriTBsub 378
http://parts.igem.org/Part:BBa_K4103007 Resistance marker catA9 939
http://parts.igem.org/Part:BBa_K4103008 Insulator HDV-1 (3' Ribozyme Insulator) 85
http://parts.igem.org/Part:BBa_K4103009
http://parts.igem.org/Part:BBa_K4103016 OriTBsub
http://parts.igem.org/Part:BBa_K4103017 Terminator E. coli terminator 54
http://parts.igem.org/Part:BBa_K4103018 Promoter kanR promoter 101
http://parts.igem.org/Part:BBa_K4103019 Terminator kanR terminator 48
http://parts.igem.org/Part:BBa_K4103020 Coding kanR cds 816
http://parts.igem.org/Part:BBa_K4103021 Coding lacI cds 1083
http://parts.igem.org/Part:BBa_K4103022
http://parts.igem.org/Part:BBa_K4103023 Operator Lac operator 25
http://parts.igem.org/Part:BBa_K4103024 Insulator RiboJ54 Insulator 75
http://parts.igem.org/Part:BBa_K4103025
http://parts.igem.org/Part:BBa_K4103026 Coding Pfu (DNA polymerase) #4 2322
http://parts.igem.org/Part:BBa_K4103027 Coding Sso-7d #4 189
http://parts.igem.org/Part:BBa_K4103028 Linker TEV linker site #4 21
http://parts.igem.org/Part:BBa_K4103029 Coding fuGFP #4 732
http://parts.igem.org/Part:BBa_K4103030 Tag 10xHisTag #4 30
http://parts.igem.org/Part:BBa_K4103031 Tag R5 Silica binding purification tag #4 57
http://parts.igem.org/Part:BBa_K4103032 Coding Pfu (DNA polymerase) #2 2322
http://parts.igem.org/Part:BBa_K4103033 Coding Sso-7d #2 189
http://parts.igem.org/Part:BBa_K4103034 Linker TEV linker site 21
http://parts.igem.org/Part:BBa_K4103035 Coding fuGFP 732
http://parts.igem.org/Part:BBa_K4103036 Purification Tag 10xHisTag 30
http://parts.igem.org/Part:BBa_K4103037 Purification Tag R5 Silica binding purification tag 57
http://parts.igem.org/Part:BBa_K4103038 Coding ccdb 335
http://parts.igem.org/Part:BBa_K4103039 Homology Arm lacA 3' homology 408
http://parts.igem.org/Part:BBa_K4103040 Homology Arm amyE 5' homology 521
http://parts.igem.org/Part:BBa_K4103041 Homology Arm amyE 3' homology 498
http://parts.igem.org/Part:BBa_K4103042 Homology Arm thrC 5' homology 501
http://parts.igem.org/Part:BBa_K4103043 Homology Arm thrC 3' homology 429
http://parts.igem.org/Part:BBa_K4103044/ Homology Arm lacA 5' homology arm 602
http://parts.igem.org/Part:BBa_K4103045 Secretion Tag AmyQ 93

Composite Parts

Part Name Type Description Length (bp)
http://parts.igem.org/Part:BBa_K4103000 pfu
http://parts.igem.org/Part:BBa_K4103001 Conjugation origin Transfer Origin pIP501 319
http://parts.igem.org/Part:BBa_K4103003 Resistance marker SpecR 989
http://parts.igem.org/Part:BBa_K4103004 Origin of replication EcoORI 589
http://parts.igem.org/Part:BBa_K4103005 Origin of transfer OriTBsub 378
http://parts.igem.org/Part:BBa_K4103007 Resistance marker catA9 939
http://parts.igem.org/Part:BBa_K4103008 Insulator HDV-1 (3' Ribozyme Insulator) 85
http://parts.igem.org/Part:BBa_K4103009
http://parts.igem.org/Part:BBa_K4103016 OriTBsub
http://parts.igem.org/Part:BBa_K4103017 Terminator E. coli terminator 54
http://parts.igem.org/Part:BBa_K4103018 Promoter kanR promoter 101
http://parts.igem.org/Part:BBa_K4103019 Terminator kanR terminator 48
http://parts.igem.org/Part:BBa_K4103020 Coding kanR cds 816
http://parts.igem.org/Part:BBa_K4103021 Coding lacI cds 1083
http://parts.igem.org/Part:BBa_K4103022
http://parts.igem.org/Part:BBa_K4103023 Operator Lac operator 25
http://parts.igem.org/Part:BBa_K4103024 Insulator RiboJ54 Insulator 75
http://parts.igem.org/Part:BBa_K4103025
http://parts.igem.org/Part:BBa_K4103026 Coding Pfu (DNA polymerase) #4 2322
http://parts.igem.org/Part:BBa_K4103027 Coding Sso-7d #4 189
http://parts.igem.org/Part:BBa_K4103028 Linker TEV linker site #4 21
http://parts.igem.org/Part:BBa_K4103029 Coding fuGFP #4 732
http://parts.igem.org/Part:BBa_K4103030 Tag 10xHisTag #4 30
http://parts.igem.org/Part:BBa_K4103031 Tag R5 Silica binding purification tag #4 57
http://parts.igem.org/Part:BBa_K4103032 Coding Pfu (DNA polymerase) #2 2322
http://parts.igem.org/Part:BBa_K4103033 Coding Sso-7d #2 189
http://parts.igem.org/Part:BBa_K4103034 Linker TEV linker site 21
http://parts.igem.org/Part:BBa_K4103035 Coding fuGFP 732
http://parts.igem.org/Part:BBa_K4103036 Purification Tag 10xHisTag 30
http://parts.igem.org/Part:BBa_K4103037 Purification Tag R5 Silica binding purification tag 57
http://parts.igem.org/Part:BBa_K4103038 Coding ccdb 335
http://parts.igem.org/Part:BBa_K4103039 Homology Arm lacA 3' homology 408
http://parts.igem.org/Part:BBa_K4103040 Homology Arm amyE 5' homology 521
http://parts.igem.org/Part:BBa_K4103041 Homology Arm amyE 3' homology 498
http://parts.igem.org/Part:BBa_K4103042 Homology Arm thrC 5' homology 501
http://parts.igem.org/Part:BBa_K4103043 Homology Arm thrC 3' homology 429
http://parts.igem.org/Part:BBa_K4103044/ Homology Arm lacA 5' homology arm 602
http://parts.igem.org/Part:BBa_K4103045 Secretion Tag AmyQ 93