Team:Open Science Global/Part Collection

Friendzymes
Part Collection
Part Collection
Overview Basic Parts Composite Parts

Overview

The main goal of our project is to make a frugal biofoundry capable of producing high quantities of enzymes and other proteins of interest. To do that, our team designed a library of parts in order to test in a high-throughput way the combinations that would generate the best results. For this year, the most important part generated by our team was the pHT43 plasmid (BBa_K4103000), an improvement on (BBa_K1074001) from 2013 - this plasmid acts as a shuttle vector between E. coli and B. subtilis, and allows the efficient production and secretion of our protein-of-interest, with our team focusing efforts to use this vector for the production of Pfu-Sso7d Polymerase (BBa_K4103011) codon optimized for B. Subtilis using the software created by our team.

Basic Parts

Part Name Type Description Length (bp)
http://parts.igem.org/Part:BBa_K4103000 pfu
http://parts.igem.org/Part:BBa_K4103001 Conjugation origin Transfer Origin pIP501 319
http://parts.igem.org/Part:BBa_K4103003 Resistance marker SpecR 989
http://parts.igem.org/Part:BBa_K4103004 Origin of replication EcoORI 589
http://parts.igem.org/Part:BBa_K4103005 Origin of transfer OriTBsub 378
http://parts.igem.org/Part:BBa_K4103007 Resistance marker catA9 939
http://parts.igem.org/Part:BBa_K4103008 Insulator HDV-1 (3' Ribozyme Insulator) 85
http://parts.igem.org/Part:BBa_K4103009
http://parts.igem.org/Part:BBa_K4103016 OriTBsub
http://parts.igem.org/Part:BBa_K4103017 Terminator E. coli terminator 54
http://parts.igem.org/Part:BBa_K4103018 Promoter kanR promoter 101
http://parts.igem.org/Part:BBa_K4103019 Terminator kanR terminator 48
http://parts.igem.org/Part:BBa_K4103020 Coding kanR cds 816
http://parts.igem.org/Part:BBa_K4103021 Coding lacI cds 1083
http://parts.igem.org/Part:BBa_K4103022
http://parts.igem.org/Part:BBa_K4103023 Operator Lac operator 25
http://parts.igem.org/Part:BBa_K4103024 Insulator RiboJ54 Insulator 75
http://parts.igem.org/Part:BBa_K4103025
http://parts.igem.org/Part:BBa_K4103026 Coding Pfu (DNA polymerase) #4 2322
http://parts.igem.org/Part:BBa_K4103027 Coding Sso-7d #4 189
http://parts.igem.org/Part:BBa_K4103028 Linker TEV linker site #4 21
http://parts.igem.org/Part:BBa_K4103029 Coding fuGFP #4 732
http://parts.igem.org/Part:BBa_K4103030 Tag 10xHisTag #4 30
http://parts.igem.org/Part:BBa_K4103031 Tag R5 Silica binding purification tag #4 57
http://parts.igem.org/Part:BBa_K4103032 Coding Pfu (DNA polymerase) #2 2322
http://parts.igem.org/Part:BBa_K4103033 Coding Sso-7d #2 189
http://parts.igem.org/Part:BBa_K4103034 Linker TEV linker site 21
http://parts.igem.org/Part:BBa_K4103035 Coding fuGFP 732
http://parts.igem.org/Part:BBa_K4103036 Purification Tag 10xHisTag 30
http://parts.igem.org/Part:BBa_K4103037 Purification Tag R5 Silica binding purification tag 57
http://parts.igem.org/Part:BBa_K4103038 Coding ccdb 335
http://parts.igem.org/Part:BBa_K4103039 Homology Arm lacA 3' homology 408
http://parts.igem.org/Part:BBa_K4103040 Homology Arm amyE 5' homology 521
http://parts.igem.org/Part:BBa_K4103041 Homology Arm amyE 3' homology 498
http://parts.igem.org/Part:BBa_K4103042 Homology Arm thrC 5' homology 501
http://parts.igem.org/Part:BBa_K4103043 Homology Arm thrC 3' homology 429
http://parts.igem.org/Part:BBa_K4103044 Homology Arm lacA 5' homology arm 602
http://parts.igem.org/Part:BBa_K4103045 Secretion Tag AmyQ 93

Composite Parts

Part Name Type Description Length (bp)
http://parts.igem.org/Part:BBa_K4103000 pfu
http://parts.igem.org/Part:BBa_K4103001 Conjugation origin Transfer Origin pIP501 319
http://parts.igem.org/Part:BBa_K4103003 Resistance marker SpecR 989
http://parts.igem.org/Part:BBa_K4103004 Origin of replication EcoORI 589
http://parts.igem.org/Part:BBa_K4103005 Origin of transfer OriTBsub 378
http://parts.igem.org/Part:BBa_K4103007 Resistance marker catA9 939
http://parts.igem.org/Part:BBa_K4103008 Insulator HDV-1 (3' Ribozyme Insulator) 85
http://parts.igem.org/Part:BBa_K4103009
http://parts.igem.org/Part:BBa_K4103016 OriTBsub
http://parts.igem.org/Part:BBa_K4103017 Terminator E. coli terminator 54
http://parts.igem.org/Part:BBa_K4103018 Promoter kanR promoter 101
http://parts.igem.org/Part:BBa_K4103019 Terminator kanR terminator 48
http://parts.igem.org/Part:BBa_K4103020 Coding kanR cds 816
http://parts.igem.org/Part:BBa_K4103021 Coding lacI cds 1083
http://parts.igem.org/Part:BBa_K4103022
http://parts.igem.org/Part:BBa_K4103023 Operator Lac operator 25
http://parts.igem.org/Part:BBa_K4103024 Insulator RiboJ54 Insulator 75
http://parts.igem.org/Part:BBa_K4103025
http://parts.igem.org/Part:BBa_K4103026 Coding Pfu (DNA polymerase) #4 2322
http://parts.igem.org/Part:BBa_K4103027 Coding Sso-7d #4 189
http://parts.igem.org/Part:BBa_K4103028 Linker TEV linker site #4 21
http://parts.igem.org/Part:BBa_K4103029 Coding fuGFP #4 732
http://parts.igem.org/Part:BBa_K4103030 Tag 10xHisTag #4 30
http://parts.igem.org/Part:BBa_K4103031 Tag R5 Silica binding purification tag #4 57
http://parts.igem.org/Part:BBa_K4103032 Coding Pfu (DNA polymerase) #2 2322
http://parts.igem.org/Part:BBa_K4103033 Coding Sso-7d #2 189
http://parts.igem.org/Part:BBa_K4103034 Linker TEV linker site 21
http://parts.igem.org/Part:BBa_K4103035 Coding fuGFP 732
http://parts.igem.org/Part:BBa_K4103036 Purification Tag 10xHisTag 30
http://parts.igem.org/Part:BBa_K4103037 Purification Tag R5 Silica binding purification tag 57
http://parts.igem.org/Part:BBa_K4103038 Coding ccdb 335
http://parts.igem.org/Part:BBa_K4103039 Homology Arm lacA 3' homology 408
http://parts.igem.org/Part:BBa_K4103040 Homology Arm amyE 5' homology 521
http://parts.igem.org/Part:BBa_K4103041 Homology Arm amyE 3' homology 498
http://parts.igem.org/Part:BBa_K4103042 Homology Arm thrC 5' homology 501
http://parts.igem.org/Part:BBa_K4103043 Homology Arm thrC 3' homology 429
http://parts.igem.org/Part:BBa_K4103044 Homology Arm lacA 5' homology arm 602
http://parts.igem.org/Part:BBa_K4103045 Secretion Tag AmyQ 93