Difference between revisions of "Team:Open Science Global/Parts"

(Prototype team page)
 
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{{IGEM_TopBar}}
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{{Open_Science_Global/main}}
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{{Open_Science_Global}}
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{{Open_Science_Global/navbar}}
 
<html>
 
<html>
 +
<style>
 +
    @import url('https://fonts.googleapis.com/css2?family=Roboto&display=swap');
  
 +
    body {
 +
        margin: 0;
 +
        background-color: #E5E5E5 !important;
 +
    }
  
−
<div class="column full_size">
+
    html {
−
<h1>Parts</h1>
+
        scroll-behavior: smooth;
−
<p>Each team will make new parts during iGEM and will add them to the Registry of Standard Biological Parts. iGEM provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
+
    }
−
<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
+
−
</div>
+
  
−
<div class="column full_size">
+
    .container {
−
<div class="highlight decoration_background">
+
        height: auto;
−
<h3>Note</h3>
+
        display: -webkit-box;
−
<p>Note that parts must be well documented on each Part's Main Page on the <a href="http://parts.igem.org/Main_Page">Registry</a>. This documentation includes all of the characterization data for your parts. <b>The part's data MUST be on the part's Main Page on the Registry for your team to be eligible for medals and special prizes pertaining to parts.</b> <br><br>
+
        display: -ms-flexbox;
−
This page serves to <i>showcase</i> the parts you have made and should include links to the Registry pages for your parts. Future teams and other users are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
+
        display: flex;
−
</div>
+
        -webkit-box-orient: horizontal;
−
</div>
+
        -webkit-box-direction: normal;
 +
        -ms-flex-direction: row;
 +
        flex-direction: row;
 +
        -ms-flex-pack: distribute;
 +
        justify-content: space-around;
 +
        -ms-flex-wrap: wrap;
 +
        flex-wrap: wrap;
 +
        -webkit-column-gap: 18px;
 +
        column-gap: 18px;
 +
    }
  
−
<div class="clear extra_space"></div>
+
    .top-banner {
−
<div class="line_divider"></div>
+
        height: 220px;
−
<div class="clear extra_space"></div>
+
        top: 16px;
 +
        background: #FF8552;
 +
    }
  
 +
    .Title {
 +
        font-family: Roboto;
 +
        text-transform: uppercase;
 +
        font-weight: bold;
 +
        font-size: 2.5vw;
 +
        -ms-flex-item-align: center;
 +
        -ms-grid-row-align: center;
 +
        align-self: center;
 +
    }
  
 +
    .Subtitle {
 +
        display: -webkit-box;
 +
        display: -ms-flexbox;
 +
        display: flex;
 +
        gap: 28vw;
 +
        height: 20vh;
 +
        justify-content: center;
 +
    }
  
 +
    .Subtitle .mini-subtitle {
 +
        display: -webkit-box;
 +
        display: -ms-flexbox;
 +
        display: flex;
 +
        -webkit-box-align: center;
 +
        -ms-flex-align: center;
 +
        align-items: center;
 +
        gap: 50px;
 +
        margin-left: 20px;
 +
    }
  
 +
    .Subtitle .mini-subtitle .main-subtitle {
 +
        font-family: Roboto;
 +
        font-style: normal;
 +
        font-weight: bold;
 +
        font-size: 1.3vw;
 +
    }
  
−
<div class="column full_size">
+
    .Subtitle .mini-subtitle .line {
−
<div class="highlight decoration_B_full">
+
        width: 2px;
 +
        height: 26px;
 +
        background: #000000;
 +
    }
  
−
<h3>Adding parts to the Registry</h3>
+
    .Subtitle .mini-subtitle .subtopic {
−
<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
+
        font-family: Roboto;
 +
        font-style: normal;
 +
        font-weight: bold;
 +
        font-size: 1vw;
 +
    }
  
−
<p>We encourage teams to start adding and documenting their parts on the Registry as soon as they can. Once you add your parts to the Registry, you can continue to add documentation to them throughout the iGEM season (up until the Registry freeze). This will allow you to remember all the details about your parts and store their history in the wiki. Documentation includes the characterization data of your parts.</p>
+
    .subsite-container {
−
<div class="button_link">
+
        display: -webkit-box;
−
<a href="http://parts.igem.org/Add_a_Part_to_the_Registry">
+
        display: -ms-flexbox;
−
ADD PARTS
+
        display: flex;
−
</a>
+
        -webkit-box-orient: vertical;
−
</div>
+
        -webkit-box-direction: normal;
 +
        -ms-flex-direction: column;
 +
        flex-direction: column;
 +
        -webkit-box-align: center;
 +
        -ms-flex-align: center;
 +
        align-items: center;
 +
        font-family: Roboto;
 +
        font-weight: bold;
 +
        -webkit-box-ordinal-group: 2;
 +
        -ms-flex-order: 1;
 +
        order: 1;
 +
        -webkit-box-flex: 2;
 +
        -ms-flex-positive: 2;
 +
        flex-grow: 2;
 +
        width: 100px;
 +
        height: 250px;
 +
        -ms-flex-negative: 1;
 +
        flex-shrink: 1;
 +
        margin-left: 7px;
 +
    }
  
−
</div>
+
    .subsite-container .submenu-title {
−
</div>
+
        font-size: 1.3vw;
 +
        margin-bottom: 7px;
 +
    }
  
 +
    .subsite-container .index-box {
 +
        display: -webkit-box;
 +
        display: -ms-flexbox;
 +
        display: flex;
 +
        -webkit-box-orient: vertical;
 +
        -webkit-box-direction: normal;
 +
        -ms-flex-direction: column;
 +
        flex-direction: column;
 +
        width: 100%;
 +
        height: 100%;
 +
        font-size: 1.1vw;
 +
        background: #F8F8F8;
 +
        border-radius: 15px;
 +
        -webkit-box-align: center;
 +
        -ms-flex-align: center;
 +
        align-items: center;
 +
        -webkit-box-pack: space-evenly;
 +
        -ms-flex-pack: space-evenly;
 +
        justify-content: space-evenly;
 +
        border-radius: 15px;
 +
    }
  
 +
    .subsite-container .index-box a {
 +
        text-decoration: none;
 +
        text-transform: uppercase;
 +
        color: black;
 +
        width: 80%;
 +
        border-bottom: 0.5px solid #DCDCDC;
 +
        text-align: center;
 +
    }
  
−
<div class="column third_size">
+
    .nav-menu {
−
<div class="highlight decoration_A_full">
+
        display: -webkit-box;
−
<h3>Inspiration: Basic Parts</h3>
+
        display: -ms-flexbox;
−
<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
+
        display: flex;
 +
        -webkit-box-orient: vertical;
 +
        -webkit-box-direction: normal;
 +
        -ms-flex-direction: column;
 +
        flex-direction: column;
 +
        font-family: Roboto;
 +
        font-weight: bold;
 +
        -webkit-box-ordinal-group: 4;
 +
        -ms-flex-order: 1;
 +
        order: 1;
 +
        -webkit-box-flex: 2;
 +
        -ms-flex-positive: 2;
 +
        flex-grow: 0.5;
 +
        -ms-flex-negative: 1;
 +
        flex-shrink: 1;
 +
        width: 100px;
 +
        height: 250px;
 +
        margin-right: 7px;
 +
        margin-left: 25px;
 +
       
 +
    }
  
−
<p> You can also take a look at the following examples for Basic Parts on the Registry:</p>
+
    .nav-menu .submenu-title {
−
<ul>
+
        font-size: 1.3vw;
−
<li><a href="http://parts.igem.org/Part:BBa_K3114006">2019 Calgary </a></li>
+
        text-align: center;
−
<li><a href="http://parts.igem.org/Part:BBa_K3027000">2019 GO Paris Saclay</a></li>
+
        text-transform: uppercase;
−
<li><a href="http://parts.igem.org/Part:BBa_K3187028">2019 TU Darmstadt</a></li>
+
        margin-bottom: 7px;
−
<li><a href="http://parts.igem.org/Part:BBa_K3552000">2020 Links China</a></li>
+
    }
−
<li><a href="http://parts.igem.org/Part:BBa_K3558000">2020 UNSW Australia</a></li>
+
−
<li><a href="http://parts.igem.org/Part:BBa_K3338002">2020 Hannover</a></li>
+
−
</ul>
+
−
</div>
+
−
</div>
+
  
−
<div class="column third_size">
+
    .nav-menu .submenu-content {
−
<div class="highlight decoration_A_full">
+
        display: -webkit-box;
−
<h3>Inspiration: Composite Parts</h3>
+
        display: -ms-flexbox;
−
<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
+
        display: flex;
 +
        -webkit-box-orient: vertical;
 +
        -webkit-box-direction: normal;
 +
        -ms-flex-direction: column;
 +
        flex-direction: column;
 +
        width: 100%;
 +
        height: 100%;
 +
        background: #F8F8F8;
 +
        border-radius: 15px;
 +
        -webkit-box-align: center;
 +
        -ms-flex-align: center;
 +
        align-items: center;
 +
        -webkit-box-pack: space-evenly;
 +
        -ms-flex-pack: space-evenly;
 +
        justify-content: space-evenly;
 +
        border-radius: 15px;
 +
    }
  
−
<p> You can also take a look at the following examples for Composite Parts on the Registry:</p>
+
    .nav-menu .submenu-content a {
−
<ul>
+
        text-decoration: none;
−
<li><a href="http://parts.igem.org/Part:BBa_K3198007">2019 NUS Singapore </a></li>
+
        color: black;
−
<li><a href="http://parts.igem.org/Part:BBa_K2932003">2019 Mingdao</a></li>
+
        font-family: Roboto;
−
<li><a href="http://parts.igem.org/Part:BBa_K2980009">2019 Tsinghua</a></li>
+
        font-size: 1.1vw;
 +
        font-weight: bold;
 +
        width: 80%;
 +
        border-bottom: 0.5px solid #DCDCDC;
 +
        text-align: center;
 +
        text-transform: uppercase;
 +
    }
  
−
<li><a href="http://parts.igem.org/Part:BBa_K3407022">2020 TUDelft</a></li>
+
    .text-container {
−
<li><a href="http://parts.igem.org/Part:BBa_K3380500">2020 Edinburgh</a></li>
+
        display: -webkit-box;
−
<li><a href="http://parts.igem.org/Part:BBa_K3512042">2020 BITSPilani Goa India</a></li>
+
        display: -ms-flexbox;
−
</ul>
+
        display: flex;
−
</div>
+
        -webkit-box-orient: vertical;
−
</div>
+
        -webkit-box-direction: normal;
 +
        -ms-flex-direction: column;
 +
        flex-direction: column;
 +
        width: 400px;
 +
        height: auto;
 +
        padding-bottom: 2%;
 +
        padding-top: 2%;
 +
        background: #F9F9F9;
 +
        border-radius: 38px;
 +
        -webkit-box-shadow: 0px 11px 15px 1px rgba(0, 0, 0, 0.65);
 +
        box-shadow: 0px 11px 15px 1px rgba(0, 0, 0, 0.65);
 +
        -webkit-box-align: start;
 +
        -ms-flex-align: start;
 +
        align-items: flex-start;
 +
        overflow: hidden;
 +
        -webkit-box-ordinal-group: 3;
 +
        -ms-flex-order: 2;
 +
        order: 2;
 +
        -webkit-box-flex: 4;
 +
        -ms-flex-positive: 4;
 +
        flex-grow: 2;
 +
        -ms-flex-negative: 0.5;
 +
        flex-shrink: 0.5;
 +
        margin-right: 50px;
 +
    }
  
 +
    .text-container h2 {
 +
        -ms-flex-item-align: start !important;
 +
        align-self: flex-start !important;
 +
        font-family: Roboto;
 +
        font-weight: bold;
 +
        font-size: 2.5vw;
 +
        height: 5vw;
 +
        width: 39vw;
 +
        letter-spacing: 0.01em;
 +
        text-decoration: none !important;
 +
        color: #000000;
 +
        margin-left: 40px;
 +
        margin-top: 35px !important;
 +
        margin-bottom: 35px !important;
 +
        border-bottom: 2.5px solid #000000;
 +
    }
  
 +
    .text-container .text p {
 +
        font-family: Roboto !important;
 +
        font-weight: normal;
 +
        font-size: 1.3vw !important;
 +
        letter-spacing: 0.01em;
 +
        color: #000000;
 +
        text-align: justify;
 +
        margin-right: 40px;
 +
        margin-left: 40px;
 +
    }
  
−
<div class="column third_size">
+
    footer {
 +
        height: 300px;
 +
        width: 100vw;
 +
        bottom: 0;
 +
        background: #1E2833;
 +
        -ms-flex-item-align: end;
 +
        align-self: flex-end;
 +
        -webkit-box-ordinal-group: 5;
 +
        -ms-flex-order: 4;
 +
        order: 4;
 +
        padding-top: 2%;
 +
        margin-top: 5%;
 +
    }
  
−
<h3>What information do I need to start putting my parts on the Registry?</h3>
+
    @media screen and (max-width: 900px) {
−
<p>The information needed to initially create a part on the Registry is:</p>
+
        .Title {
−
<ul>
+
            font-size: 3vw;
−
<li>Part Name</li>
+
        }
−
<li>Part type</li>
+
−
<li>Creator</li>
+
−
<li>Sequence</li>
+
−
<li>Short Description (60 characters on what the DNA does)</li>
+
−
<li>Long Description (Longer description of what the DNA does)</li>
+
−
<li>Design considerations</li>
+
−
</ul>
+
  
−
<p>
+
        .Subtitle .mini-subtitle .main-subtitle {
−
We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, you must also put it up on the part page. </p>
+
            font-size: 2.5vw;
 +
        }
  
−
</div>
+
        .Subtitle .mini-subtitle .subtopic {
 +
            font-size: 2vw;
 +
        }
  
 +
        .nav-menu {
 +
            display: none;
 +
        }
  
−
<div class="clear extra_space"></div>
+
        .subsite-container {
−
<div class="line_divider"></div>
+
            display: none;
−
<div class="clear extra_space"></div>
+
        }
  
−
<div class="column full_size">
+
        .text-container {
−
<h3>Part Table </h3>
+
            margin-left: 20px;
 +
            margin-right: 20px;
 +
        }
  
−
<p>Please include a table of all the parts your team has made during your project on this page. Remember part characterization and measurement data must go on your team part pages on the Registry. </p>
+
        .text-container .text {
 +
            font-size: 2vw;
 +
        }
  
−
</html>
+
        h2 {
−
<groupparts>iGEM20 Open_Science_Global</groupparts>
+
            font-size: 4vw;
−
<html>
+
        }
−
</div>
+
    }
 +
 
 +
    @media screen and (max-width: 600px) {
 +
        .Title {
 +
            font-size: 4vw;
 +
        }
 +
 
 +
        .Subtitle .mini-subtitle .main-subtitle {
 +
            font-size: 2.5vw;
 +
        }
 +
 
 +
        .Subtitle .mini-subtitle .subtopic {
 +
            font-size: 2vw;
 +
        }
 +
 
 +
        .nav-menu {
 +
            display: none;
 +
        }
 +
 
 +
        .subsite-container {
 +
            display: none;
 +
        }
 +
 
 +
        .text-container {
 +
            margin-left: 20px;
 +
            margin-right: 20px;
 +
        }
 +
 
 +
        .text-container .text {
 +
            font-size: 3vw;
 +
        }
 +
 
 +
        h2 {
 +
            font-size: 6vw;
 +
        }
 +
    }
 +
</style>
 +
<!-------------------- HTML CONTENT -------------------------------->
 +
 
 +
<body>
 +
    <div class="top-banner">
 +
    </div>
 +
 
 +
    <div class="Subtitle">
 +
        <div class="Title">Part Collection</div>
 +
    </div>
 +
    <div class="container">
 +
 
 +
 
 +
 
 +
        <div class="nav-menu">
 +
            <div class="submenu-title">Part Collection</div>
 +
 
 +
            <div class="submenu-content">
 +
                <a href="#Overview">Overview</a>
 +
                <a href="#Basic Parts">Basic Parts</a>
 +
                <a href="#Composite Parts">Composite Parts</a>
 +
            </div>
 +
 
 +
        </div>
 +
 
 +
        <div class="text-container">
 +
            <h2 id="Overview">Overview</h2>
 +
            <div class="text">
 +
                <p>
 +
                    The main goal of our project is to make a frugal biofoundry capable of producing high quantities of enzymes and other proteins of interest. To do that, our team designed a library of parts in order to test in a high-throughput way the combinations that would generate the best results. For this year, the most important part generated by our team was the pHT43 plasmid (BBa_K4103000), an improvement on (BBa_K1074001) from 2013 - this plasmid acts as a shuttle vector between E. coli and B. subtilis, and allows the efficient production and secretion of our protein-of-interest, with our team focusing efforts to use this vector for the production of Pfu-Sso7d Polymerase (BBa_K4103011) codon optimized for B. Subtilis using the software created by our team.
 +
                </p>
 +
            </div>
 +
 
 +
            <h2 id="Basic Parts">Basic Parts</h2>
 +
 
 +
            <style type="text/css">
 +
                .tg  {border-collapse:collapse;border-color:#ccc;border-spacing:0;}
 +
                .tg td{background-color:#fff;border-color:#ccc;border-style:solid;border-width:1px;color:#333;
 +
                  font-family:Roboto, Roboto;font-size:14px;overflow:hidden;padding:10px 5px;word-break:normal;}
 +
                .tg th{background-color:#f0f0f0;border-color:#ccc;border-style:solid;border-width:1px;color:#333;
 +
                  font-family:Roboto, Roboto;font-size:14px;font-weight:normal;overflow:hidden;padding:10px 5px;word-break:normal;}
 +
                .tg .tg-3rmm{background-color:#ffffff;border-color:inherit;color:#15C;text-align:left;text-decoration:underline;vertical-align:top}
 +
                .tg .tg-lp5f{background-color:#efefef;border-color:inherit;font-family:"Roboto Black", Gadget, Roboto !important;;
 +
                  text-align:center;vertical-align:top}
 +
                .tg .tg-3xi5{background-color:#ffffff;border-color:inherit;text-align:center;vertical-align:top}
 +
                .tg .tg-c6of{background-color:#ffffff;border-color:inherit;text-align:left;vertical-align:top}
 +
                .tg{
 +
                    align-self: center;
 +
                    width: 80%;
 +
                }
 +
                </style>
 +
                <table class="tg">
 +
                <thead>
 +
                  <tr>
 +
                    <th class="tg-lp5f"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Part Name</span></th>
 +
                    <th class="tg-lp5f"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Type</span></th>
 +
                    <th class="tg-lp5f"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Description</span></th>
 +
                    <th class="tg-lp5f"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Length (bp)</span></th>
 +
                  </tr>
 +
                </thead>
 +
                <tbody>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103000" target="_blank" rel="noopener noreferrer"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103000</span></a></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">pfu</span></td>
 +
                    <td class="tg-c6of"></td>
 +
                    <td class="tg-c6of"></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103001" target="_blank" rel="noopener noreferrer"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103001</span></a></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Conjugation origin</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Transfer Origin pIP501</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">319</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103003</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Resistance marker</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">SpecR</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">989</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103004</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Origin of replication</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">EcoORI</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">589</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103005" target="_blank" rel="noopener noreferrer"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103005</span></a></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Origin of transfer</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">OriTBsub</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">378</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103007" target="_blank" rel="noopener noreferrer"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103007</span></a></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Resistance marker</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">catA9</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">939</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103008</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Insulator</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">HDV-1 (3' Ribozyme Insulator)</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">85</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103009</span></td>
 +
                    <td class="tg-c6of"></td>
 +
                    <td class="tg-c6of"></td>
 +
                    <td class="tg-c6of"></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103016</span></td>
 +
                    <td class="tg-c6of"></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">OriTBsub</span></td>
 +
                    <td class="tg-c6of"></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103017</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Terminator</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">E. coli terminator</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">54</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103018</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Promoter</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">kanR promoter</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">101</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103019" target="_blank" rel="noopener noreferrer"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103019</span></a></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Terminator</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">kanR terminator</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">48</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103020</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">kanR cds</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">816</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103021</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">lacI cds</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">1083</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103022</span></td>
 +
                    <td class="tg-c6of"></td>
 +
                    <td class="tg-c6of"></td>
 +
                    <td class="tg-c6of"></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103023</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Operator</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Lac operator</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">25</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103024</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Insulator</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">RiboJ54 Insulator</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">75</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103025</span></td>
 +
                    <td class="tg-c6of"></td>
 +
                    <td class="tg-c6of"></td>
 +
                    <td class="tg-c6of"></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103026</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Pfu (DNA polymerase) #4</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">2322</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103027</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Sso-7d #4</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">189</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103028</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Linker</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">TEV linker site #4</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">21</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103029</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">fuGFP #4</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">732</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103030</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Tag</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">10xHisTag #4</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">30</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103031</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Tag</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">R5 Silica binding purification tag #4</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">57</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103032</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Pfu (DNA polymerase) #2</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">2322</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103033</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Sso-7d #2</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">189</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103034</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Linker</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">TEV linker site</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">21</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103035</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">fuGFP</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">732</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103036</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Purification Tag</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">10xHisTag</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">30</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103037</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Purification Tag</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">R5 Silica binding purification tag</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">57</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103038</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">ccdb</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">335</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103039</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">lacA 3' homology</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">408</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103040</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">amyE 5' homology</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">521</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103041</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">amyE 3' homology</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">498</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103042</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">thrC 5' homology</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">501</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103043</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">thrC 3' homology</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">429</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103044" target="_blank" rel="noopener noreferrer"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103044</span></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">/</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">lacA 5' homology arm</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">602</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103045</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Secretion Tag</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">AmyQ</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">93</span></td>
 +
                  </tr>
 +
                </tbody>
 +
                </table>
 +
           
 +
 
 +
 
 +
            <h2 id="Composite Parts">Composite Parts</h2>
 +
            <style type="text/css">
 +
                .tg  {border-collapse:collapse;border-color:#ccc;border-spacing:0;}
 +
                .tg td{background-color:#fff;border-color:#ccc;border-style:solid;border-width:1px;color:#333;
 +
                  font-family:Roboto, Roboto;font-size:14px;overflow:hidden;padding:10px 5px;word-break:normal;}
 +
                .tg th{background-color:#f0f0f0;border-color:#ccc;border-style:solid;border-width:1px;color:#333;
 +
                  font-family:Roboto, Roboto;font-size:14px;font-weight:normal;overflow:hidden;padding:10px 5px;word-break:normal;}
 +
                .tg .tg-3rmm{background-color:#ffffff;border-color:inherit;color:#15C;text-align:left;text-decoration:underline;vertical-align:top}
 +
                .tg .tg-lp5f{background-color:#efefef;border-color:inherit;font-family:"Roboto Black", Gadget, Roboto !important;;
 +
                  text-align:center;vertical-align:top}
 +
                .tg .tg-3xi5{background-color:#ffffff;border-color:inherit;text-align:center;vertical-align:top}
 +
                .tg .tg-c6of{background-color:#ffffff;border-color:inherit;text-align:left;vertical-align:top}
 +
                .tg{
 +
                    align-self: center;
 +
                    width: 80%;
 +
                }
 +
 
 +
                </style>
 +
                <table class="tg">
 +
                <thead>
 +
                  <tr>
 +
                    <th class="tg-lp5f"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Part Name</span></th>
 +
                    <th class="tg-lp5f"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Type</span></th>
 +
                    <th class="tg-lp5f"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Description</span></th>
 +
                    <th class="tg-lp5f"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Length (bp)</span></th>
 +
                  </tr>
 +
                </thead>
 +
                <tbody>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103000" target="_blank" rel="noopener noreferrer"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103000</span></a></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">pfu</span></td>
 +
                    <td class="tg-c6of"></td>
 +
                    <td class="tg-c6of"></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103001" target="_blank" rel="noopener noreferrer"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103001</span></a></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Conjugation origin</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Transfer Origin pIP501</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">319</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103003</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Resistance marker</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">SpecR</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">989</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103004</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Origin of replication</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">EcoORI</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">589</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103005" target="_blank" rel="noopener noreferrer"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103005</span></a></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Origin of transfer</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">OriTBsub</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">378</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103007" target="_blank" rel="noopener noreferrer"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103007</span></a></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Resistance marker</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">catA9</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">939</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103008</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Insulator</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">HDV-1 (3' Ribozyme Insulator)</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">85</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103009</span></td>
 +
                    <td class="tg-c6of"></td>
 +
                    <td class="tg-c6of"></td>
 +
                    <td class="tg-c6of"></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103016</span></td>
 +
                    <td class="tg-c6of"></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">OriTBsub</span></td>
 +
                    <td class="tg-c6of"></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103017</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Terminator</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">E. coli terminator</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">54</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103018</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Promoter</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">kanR promoter</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">101</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103019" target="_blank" rel="noopener noreferrer"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103019</span></a></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Terminator</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">kanR terminator</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">48</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103020</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">kanR cds</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">816</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103021</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">lacI cds</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">1083</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103022</span></td>
 +
                    <td class="tg-c6of"></td>
 +
                    <td class="tg-c6of"></td>
 +
                    <td class="tg-c6of"></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103023</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Operator</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Lac operator</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">25</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103024</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Insulator</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">RiboJ54 Insulator</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">75</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103025</span></td>
 +
                    <td class="tg-c6of"></td>
 +
                    <td class="tg-c6of"></td>
 +
                    <td class="tg-c6of"></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103026</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Pfu (DNA polymerase) #4</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">2322</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103027</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Sso-7d #4</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">189</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103028</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Linker</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">TEV linker site #4</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">21</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103029</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">fuGFP #4</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">732</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103030</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Tag</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">10xHisTag #4</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">30</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103031</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Tag</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">R5 Silica binding purification tag #4</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">57</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103032</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Pfu (DNA polymerase) #2</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">2322</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103033</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Sso-7d #2</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">189</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103034</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Linker</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">TEV linker site</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">21</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103035</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">fuGFP</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">732</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103036</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Purification Tag</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">10xHisTag</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">30</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103037</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Purification Tag</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">R5 Silica binding purification tag</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">57</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103038</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">ccdb</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">335</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103039</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">lacA 3' homology</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">408</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103040</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">amyE 5' homology</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">521</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103041</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">amyE 3' homology</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">498</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103042</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">thrC 5' homology</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">501</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103043</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">thrC 3' homology</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">429</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103044" target="_blank" rel="noopener noreferrer"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103044</span></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">/</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">lacA 5' homology arm</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">602</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103045</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Secretion Tag</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">AmyQ</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">93</span></td>
 +
                  </tr>
 +
                </tbody>
 +
                </table>
 +
 
 +
 
 +
 
 +
 
 +
        </div>
 +
 
 +
 
 +
           
  
  
 +
        <footer>
  
 +
        </footer>
 +
    </div>
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</body>
  
 
</html>
 
</html>

Revision as of 15:12, 21 October 2021

Friendzymes
Part Collection
Part Collection
Overview Basic Parts Composite Parts

Overview

The main goal of our project is to make a frugal biofoundry capable of producing high quantities of enzymes and other proteins of interest. To do that, our team designed a library of parts in order to test in a high-throughput way the combinations that would generate the best results. For this year, the most important part generated by our team was the pHT43 plasmid (BBa_K4103000), an improvement on (BBa_K1074001) from 2013 - this plasmid acts as a shuttle vector between E. coli and B. subtilis, and allows the efficient production and secretion of our protein-of-interest, with our team focusing efforts to use this vector for the production of Pfu-Sso7d Polymerase (BBa_K4103011) codon optimized for B. Subtilis using the software created by our team.

Basic Parts

Part Name Type Description Length (bp)
http://parts.igem.org/Part:BBa_K4103000 pfu
http://parts.igem.org/Part:BBa_K4103001 Conjugation origin Transfer Origin pIP501 319
http://parts.igem.org/Part:BBa_K4103003 Resistance marker SpecR 989
http://parts.igem.org/Part:BBa_K4103004 Origin of replication EcoORI 589
http://parts.igem.org/Part:BBa_K4103005 Origin of transfer OriTBsub 378
http://parts.igem.org/Part:BBa_K4103007 Resistance marker catA9 939
http://parts.igem.org/Part:BBa_K4103008 Insulator HDV-1 (3' Ribozyme Insulator) 85
http://parts.igem.org/Part:BBa_K4103009
http://parts.igem.org/Part:BBa_K4103016 OriTBsub
http://parts.igem.org/Part:BBa_K4103017 Terminator E. coli terminator 54
http://parts.igem.org/Part:BBa_K4103018 Promoter kanR promoter 101
http://parts.igem.org/Part:BBa_K4103019 Terminator kanR terminator 48
http://parts.igem.org/Part:BBa_K4103020 Coding kanR cds 816
http://parts.igem.org/Part:BBa_K4103021 Coding lacI cds 1083
http://parts.igem.org/Part:BBa_K4103022
http://parts.igem.org/Part:BBa_K4103023 Operator Lac operator 25
http://parts.igem.org/Part:BBa_K4103024 Insulator RiboJ54 Insulator 75
http://parts.igem.org/Part:BBa_K4103025
http://parts.igem.org/Part:BBa_K4103026 Coding Pfu (DNA polymerase) #4 2322
http://parts.igem.org/Part:BBa_K4103027 Coding Sso-7d #4 189
http://parts.igem.org/Part:BBa_K4103028 Linker TEV linker site #4 21
http://parts.igem.org/Part:BBa_K4103029 Coding fuGFP #4 732
http://parts.igem.org/Part:BBa_K4103030 Tag 10xHisTag #4 30
http://parts.igem.org/Part:BBa_K4103031 Tag R5 Silica binding purification tag #4 57
http://parts.igem.org/Part:BBa_K4103032 Coding Pfu (DNA polymerase) #2 2322
http://parts.igem.org/Part:BBa_K4103033 Coding Sso-7d #2 189
http://parts.igem.org/Part:BBa_K4103034 Linker TEV linker site 21
http://parts.igem.org/Part:BBa_K4103035 Coding fuGFP 732
http://parts.igem.org/Part:BBa_K4103036 Purification Tag 10xHisTag 30
http://parts.igem.org/Part:BBa_K4103037 Purification Tag R5 Silica binding purification tag 57
http://parts.igem.org/Part:BBa_K4103038 Coding ccdb 335
http://parts.igem.org/Part:BBa_K4103039 Homology Arm lacA 3' homology 408
http://parts.igem.org/Part:BBa_K4103040 Homology Arm amyE 5' homology 521
http://parts.igem.org/Part:BBa_K4103041 Homology Arm amyE 3' homology 498
http://parts.igem.org/Part:BBa_K4103042 Homology Arm thrC 5' homology 501
http://parts.igem.org/Part:BBa_K4103043 Homology Arm thrC 3' homology 429
http://parts.igem.org/Part:BBa_K4103044/ Homology Arm lacA 5' homology arm 602
http://parts.igem.org/Part:BBa_K4103045 Secretion Tag AmyQ 93

Composite Parts

Part Name Type Description Length (bp)
http://parts.igem.org/Part:BBa_K4103000 pfu
http://parts.igem.org/Part:BBa_K4103001 Conjugation origin Transfer Origin pIP501 319
http://parts.igem.org/Part:BBa_K4103003 Resistance marker SpecR 989
http://parts.igem.org/Part:BBa_K4103004 Origin of replication EcoORI 589
http://parts.igem.org/Part:BBa_K4103005 Origin of transfer OriTBsub 378
http://parts.igem.org/Part:BBa_K4103007 Resistance marker catA9 939
http://parts.igem.org/Part:BBa_K4103008 Insulator HDV-1 (3' Ribozyme Insulator) 85
http://parts.igem.org/Part:BBa_K4103009
http://parts.igem.org/Part:BBa_K4103016 OriTBsub
http://parts.igem.org/Part:BBa_K4103017 Terminator E. coli terminator 54
http://parts.igem.org/Part:BBa_K4103018 Promoter kanR promoter 101
http://parts.igem.org/Part:BBa_K4103019 Terminator kanR terminator 48
http://parts.igem.org/Part:BBa_K4103020 Coding kanR cds 816
http://parts.igem.org/Part:BBa_K4103021 Coding lacI cds 1083
http://parts.igem.org/Part:BBa_K4103022
http://parts.igem.org/Part:BBa_K4103023 Operator Lac operator 25
http://parts.igem.org/Part:BBa_K4103024 Insulator RiboJ54 Insulator 75
http://parts.igem.org/Part:BBa_K4103025
http://parts.igem.org/Part:BBa_K4103026 Coding Pfu (DNA polymerase) #4 2322
http://parts.igem.org/Part:BBa_K4103027 Coding Sso-7d #4 189
http://parts.igem.org/Part:BBa_K4103028 Linker TEV linker site #4 21
http://parts.igem.org/Part:BBa_K4103029 Coding fuGFP #4 732
http://parts.igem.org/Part:BBa_K4103030 Tag 10xHisTag #4 30
http://parts.igem.org/Part:BBa_K4103031 Tag R5 Silica binding purification tag #4 57
http://parts.igem.org/Part:BBa_K4103032 Coding Pfu (DNA polymerase) #2 2322
http://parts.igem.org/Part:BBa_K4103033 Coding Sso-7d #2 189
http://parts.igem.org/Part:BBa_K4103034 Linker TEV linker site 21
http://parts.igem.org/Part:BBa_K4103035 Coding fuGFP 732
http://parts.igem.org/Part:BBa_K4103036 Purification Tag 10xHisTag 30
http://parts.igem.org/Part:BBa_K4103037 Purification Tag R5 Silica binding purification tag 57
http://parts.igem.org/Part:BBa_K4103038 Coding ccdb 335
http://parts.igem.org/Part:BBa_K4103039 Homology Arm lacA 3' homology 408
http://parts.igem.org/Part:BBa_K4103040 Homology Arm amyE 5' homology 521
http://parts.igem.org/Part:BBa_K4103041 Homology Arm amyE 3' homology 498
http://parts.igem.org/Part:BBa_K4103042 Homology Arm thrC 5' homology 501
http://parts.igem.org/Part:BBa_K4103043 Homology Arm thrC 3' homology 429
http://parts.igem.org/Part:BBa_K4103044/ Homology Arm lacA 5' homology arm 602
http://parts.igem.org/Part:BBa_K4103045 Secretion Tag AmyQ 93