Difference between revisions of "Team:Open Science Global/Part Collection"

m (Deleted alert, so now page can be evaluated (editor: YK))
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{{IGEM_TopBar}}
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{{Open_Science_Global/main}}
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{{Open_Science_Global}}
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{{Open_Science_Global/navbar}}
 
<html>
 
<html>
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<style>
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 +
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 +
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<div class="column full_size">
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    .Title {
 +
        font-family: Roboto;
 +
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 +
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<h1> Part Collection </h1>
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    .Subtitle {
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<p>Did your team make a lot of great parts? Is there a theme that ties all your parts together? Do you have more than 10 parts in this collection? Did you make a CRISPR collection, a MoClo collection, or a collection of awesome pigment parts? Describe your parts collection on this page, so the judges can evaluate you for the Best Part Collection award.</p>
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        justify-content: center;
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<p>
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    .Subtitle .mini-subtitle {
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While you should put all the characterization information for your Parts on the Part's Main Page on the <a href="http://parts.igem.org/Main_Page">Registry</a>, you are encouraged to explain how all your parts form a collection on this page.  
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        display: -webkit-box;
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</p>
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        display: -ms-flexbox;
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 +
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 +
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<div class="column full_size">
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    .Subtitle .mini-subtitle .line {
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<div class="highlight decoration_background">
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        width: 2px;
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<h3>Note</h3>
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        height: 26px;
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<p>This page should list all the parts in the collection your team made during your project and include direct links to your Parts main pages on the Registry. <b>You must add all characterization information for your parts on Parts Main Page on the Registry.</b> You should <b>not</b> put characterization information on this page.
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        background: #000000;
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</p>
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    }
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</div>
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</div>
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 +
    .Subtitle .mini-subtitle .subtopic {
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<div class="column full_size">
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    .subsite-container {
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<div class="highlight decoration_B_full">
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<h3>Adding parts to the Registry</h3>
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    .subsite-container .submenu-title {
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<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
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        font-size: 1.3vw;
 +
        margin-bottom: 7px;
 +
    }
  
−
<p>We encourage teams to start adding and documenting their parts on the Registry as soon as they can. Once you add your parts to the Registry, you can continue to add documentation to them throughout the iGEM season (up until the Registry freeze). This will allow you to remember all the details about your parts and store their history in the wiki. Documentation includes the characterization data of your parts.</p>
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    .subsite-container .index-box {
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<div class="button_link">
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        display: -webkit-box;
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<a href="http://parts.igem.org/Add_a_Part_to_the_Registry">
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        display: -ms-flexbox;
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ADD PARTS
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        display: flex;
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</a>
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        -webkit-box-orient: vertical;
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    .subsite-container .index-box a {
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        text-align: center;
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−
<div class="column full_size">
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    .nav-menu {
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<h3>Best Part Collection Special Prize</h3>
+
        display: -webkit-box;
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<p> To be eligible for this award, each part in the collection must be well documented on the Part's Main Page on the <a href="http://parts.igem.org/Main_Page">Registry</a>. If you have a collection of parts you wish to nominate for this <a href="https://2021.igem.org/Judging/Awards">special prize</a>, make sure you add your part numbers to your <a href="https://2021.igem.org/Judging/Judging_Form">judging form</a> and delete the alert box at the top of this page.</p>
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        display: -ms-flexbox;
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<br><br>
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        -webkit-box-orient: vertical;
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        order: 1;
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        margin-left: 25px;
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    }
  
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        margin-right: 40px;
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        margin-left: 40px;
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        padding-top: 2%;
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        margin-top: 5%;
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    @media screen and (max-width: 900px) {
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</style>
 +
<!-------------------- HTML CONTENT -------------------------------->
 +
 +
<body>
 +
    <div class="top-banner">
 +
    </div>
 +
 +
    <div class="Subtitle">
 +
        <div class="Title">Part Collection</div>
 +
    </div>
 +
    <div class="container">
 +
 +
 +
 +
        <div class="nav-menu">
 +
            <div class="submenu-title">Part Collection</div>
 +
 +
            <div class="submenu-content">
 +
                <a href="#Overview">Overview</a>
 +
                <a href="#Basic Parts">Basic Parts</a>
 +
                <a href="#Composite Parts">Composite Parts</a>
 +
            </div>
 +
 +
        </div>
 +
 +
        <div class="text-container">
 +
            <h2 id="Overview">Overview</h2>
 +
            <div class="text">
 +
                <p>
 +
                    The main goal of our project is to make a frugal biofoundry capable of producing high quantities of enzymes and other proteins of interest. To do that, our team designed a library of parts in order to test in a high-throughput way the combinations that would generate the best results. For this year, the most important part generated by our team was the pHT43 plasmid (BBa_K4103000), an improvement on (BBa_K1074001) from 2013 - this plasmid acts as a shuttle vector between E. coli and B. subtilis, and allows the efficient production and secretion of our protein-of-interest, with our team focusing efforts to use this vector for the production of Pfu-Sso7d Polymerase (BBa_K4103011) codon optimized for B. Subtilis using the software created by our team.
 +
                </p>
 +
            </div>
 +
 +
            <h2 id="Basic Parts">Basic Parts</h2>
 +
 +
            <style type="text/css">
 +
                .tg  {border-collapse:collapse;border-color:#ccc;border-spacing:0;}
 +
                .tg td{background-color:#fff;border-color:#ccc;border-style:solid;border-width:1px;color:#333;
 +
                  font-family:Roboto, Roboto;font-size:14px;overflow:hidden;padding:10px 5px;word-break:normal;}
 +
                .tg th{background-color:#f0f0f0;border-color:#ccc;border-style:solid;border-width:1px;color:#333;
 +
                  font-family:Roboto, Roboto;font-size:14px;font-weight:normal;overflow:hidden;padding:10px 5px;word-break:normal;}
 +
                .tg .tg-3rmm{background-color:#ffffff;border-color:inherit;color:#15C;text-align:left;text-decoration:underline;vertical-align:top}
 +
                .tg .tg-lp5f{background-color:#efefef;border-color:inherit;font-family:"Roboto Black", Gadget, Roboto !important;;
 +
                  text-align:center;vertical-align:top}
 +
                .tg .tg-3xi5{background-color:#ffffff;border-color:inherit;text-align:center;vertical-align:top}
 +
                .tg .tg-c6of{background-color:#ffffff;border-color:inherit;text-align:left;vertical-align:top}
 +
                .tg{
 +
                    align-self: center;
 +
                    width: 80%;
 +
                }
 +
                </style>
 +
                <table class="tg">
 +
                <thead>
 +
                  <tr>
 +
                    <th class="tg-lp5f"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Part Name</span></th>
 +
                    <th class="tg-lp5f"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Type</span></th>
 +
                    <th class="tg-lp5f"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Description</span></th>
 +
                    <th class="tg-lp5f"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Length (bp)</span></th>
 +
                  </tr>
 +
                </thead>
 +
                <tbody>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103000" target="_blank" rel="noopener noreferrer"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103000</span></a></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">pfu</span></td>
 +
                    <td class="tg-c6of"></td>
 +
                    <td class="tg-c6of"></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103001" target="_blank" rel="noopener noreferrer"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103001</span></a></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Conjugation origin</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Transfer Origin pIP501</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">319</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103003</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Resistance marker</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">SpecR</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">989</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103004</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Origin of replication</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">EcoORI</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">589</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103005" target="_blank" rel="noopener noreferrer"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103005</span></a></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Origin of transfer</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">OriTBsub</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">378</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103007" target="_blank" rel="noopener noreferrer"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103007</span></a></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Resistance marker</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">catA9</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">939</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103008</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Insulator</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">HDV-1 (3' Ribozyme Insulator)</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">85</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103009</span></td>
 +
                    <td class="tg-c6of"></td>
 +
                    <td class="tg-c6of"></td>
 +
                    <td class="tg-c6of"></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103016</span></td>
 +
                    <td class="tg-c6of"></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">OriTBsub</span></td>
 +
                    <td class="tg-c6of"></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103017</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Terminator</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">E. coli terminator</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">54</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103018</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Promoter</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">kanR promoter</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">101</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103019" target="_blank" rel="noopener noreferrer"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103019</span></a></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Terminator</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">kanR terminator</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">48</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103020</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">kanR cds</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">816</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103021</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">lacI cds</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">1083</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103022</span></td>
 +
                    <td class="tg-c6of"></td>
 +
                    <td class="tg-c6of"></td>
 +
                    <td class="tg-c6of"></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103023</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Operator</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Lac operator</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">25</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103024</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Insulator</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">RiboJ54 Insulator</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">75</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103025</span></td>
 +
                    <td class="tg-c6of"></td>
 +
                    <td class="tg-c6of"></td>
 +
                    <td class="tg-c6of"></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103026</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Pfu (DNA polymerase) #4</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">2322</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103027</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Sso-7d #4</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">189</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103028</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Linker</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">TEV linker site #4</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">21</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103029</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">fuGFP #4</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">732</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103030</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Tag</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">10xHisTag #4</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">30</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103031</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Tag</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">R5 Silica binding purification tag #4</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">57</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103032</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Pfu (DNA polymerase) #2</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">2322</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103033</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Sso-7d #2</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">189</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103034</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Linker</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">TEV linker site</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">21</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103035</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">fuGFP</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">732</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103036</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Purification Tag</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">10xHisTag</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">30</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103037</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Purification Tag</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">R5 Silica binding purification tag</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">57</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103038</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">ccdb</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">335</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103039</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">lacA 3' homology</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">408</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103040</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">amyE 5' homology</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">521</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103041</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">amyE 3' homology</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">498</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103042</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">thrC 5' homology</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">501</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103043</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">thrC 3' homology</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">429</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103044" target="_blank" rel="noopener noreferrer"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103044</span></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">/</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">lacA 5' homology arm</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">602</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103045</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Secretion Tag</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">AmyQ</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">93</span></td>
 +
                  </tr>
 +
                </tbody>
 +
                </table>
 +
           
 +
 +
 +
            <h2 id="Composite Parts">Composite Parts</h2>
 +
            <style type="text/css">
 +
                .tg  {border-collapse:collapse;border-color:#ccc;border-spacing:0;}
 +
                .tg td{background-color:#fff;border-color:#ccc;border-style:solid;border-width:1px;color:#333;
 +
                  font-family:Roboto, Roboto;font-size:14px;overflow:hidden;padding:10px 5px;word-break:normal;}
 +
                .tg th{background-color:#f0f0f0;border-color:#ccc;border-style:solid;border-width:1px;color:#333;
 +
                  font-family:Roboto, Roboto;font-size:14px;font-weight:normal;overflow:hidden;padding:10px 5px;word-break:normal;}
 +
                .tg .tg-3rmm{background-color:#ffffff;border-color:inherit;color:#15C;text-align:left;text-decoration:underline;vertical-align:top}
 +
                .tg .tg-lp5f{background-color:#efefef;border-color:inherit;font-family:"Roboto Black", Gadget, Roboto !important;;
 +
                  text-align:center;vertical-align:top}
 +
                .tg .tg-3xi5{background-color:#ffffff;border-color:inherit;text-align:center;vertical-align:top}
 +
                .tg .tg-c6of{background-color:#ffffff;border-color:inherit;text-align:left;vertical-align:top}
 +
                .tg{
 +
                    align-self: center;
 +
                    width: 80%;
 +
                }
 +
 +
                </style>
 +
                <table class="tg">
 +
                <thead>
 +
                  <tr>
 +
                    <th class="tg-lp5f"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Part Name</span></th>
 +
                    <th class="tg-lp5f"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Type</span></th>
 +
                    <th class="tg-lp5f"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Description</span></th>
 +
                    <th class="tg-lp5f"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Length (bp)</span></th>
 +
                  </tr>
 +
                </thead>
 +
                <tbody>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103000" target="_blank" rel="noopener noreferrer"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103000</span></a></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">pfu</span></td>
 +
                    <td class="tg-c6of"></td>
 +
                    <td class="tg-c6of"></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103001" target="_blank" rel="noopener noreferrer"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103001</span></a></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Conjugation origin</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Transfer Origin pIP501</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">319</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103003</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Resistance marker</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">SpecR</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">989</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103004</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Origin of replication</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">EcoORI</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">589</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103005" target="_blank" rel="noopener noreferrer"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103005</span></a></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Origin of transfer</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">OriTBsub</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">378</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103007" target="_blank" rel="noopener noreferrer"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103007</span></a></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Resistance marker</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">catA9</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">939</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103008</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Insulator</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">HDV-1 (3' Ribozyme Insulator)</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">85</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103009</span></td>
 +
                    <td class="tg-c6of"></td>
 +
                    <td class="tg-c6of"></td>
 +
                    <td class="tg-c6of"></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103016</span></td>
 +
                    <td class="tg-c6of"></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">OriTBsub</span></td>
 +
                    <td class="tg-c6of"></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103017</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Terminator</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">E. coli terminator</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">54</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103018</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Promoter</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">kanR promoter</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">101</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103019" target="_blank" rel="noopener noreferrer"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103019</span></a></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Terminator</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">kanR terminator</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">48</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103020</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">kanR cds</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">816</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103021</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">lacI cds</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">1083</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103022</span></td>
 +
                    <td class="tg-c6of"></td>
 +
                    <td class="tg-c6of"></td>
 +
                    <td class="tg-c6of"></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103023</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Operator</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Lac operator</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">25</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103024</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Insulator</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">RiboJ54 Insulator</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">75</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103025</span></td>
 +
                    <td class="tg-c6of"></td>
 +
                    <td class="tg-c6of"></td>
 +
                    <td class="tg-c6of"></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103026</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Pfu (DNA polymerase) #4</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">2322</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103027</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Sso-7d #4</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">189</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103028</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Linker</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">TEV linker site #4</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">21</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103029</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">fuGFP #4</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">732</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103030</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Tag</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">10xHisTag #4</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">30</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103031</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Tag</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">R5 Silica binding purification tag #4</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">57</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103032</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Pfu (DNA polymerase) #2</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">2322</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103033</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Sso-7d #2</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">189</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103034</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Linker</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">TEV linker site</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">21</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103035</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">fuGFP</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">732</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103036</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Purification Tag</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">10xHisTag</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">30</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103037</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Purification Tag</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">R5 Silica binding purification tag</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">57</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103038</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">ccdb</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">335</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103039</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">lacA 3' homology</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">408</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103040</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">amyE 5' homology</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">521</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103041</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">amyE 3' homology</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">498</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103042</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">thrC 5' homology</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">501</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103043</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">thrC 3' homology</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">429</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103044" target="_blank" rel="noopener noreferrer"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103044</span></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">/</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">lacA 5' homology arm</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">602</span></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103045</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Secretion Tag</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">AmyQ</span></td>
 +
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">93</span></td>
 +
                  </tr>
 +
                </tbody>
 +
                </table>
 +
 +
        </div>           
 +
 +
        <footer>
 +
 +
        </footer>
 +
    </div>
 +
</body>
  
 
</html>
 
</html>

Revision as of 15:08, 21 October 2021

Friendzymes
Part Collection
Part Collection
Overview Basic Parts Composite Parts

Overview

The main goal of our project is to make a frugal biofoundry capable of producing high quantities of enzymes and other proteins of interest. To do that, our team designed a library of parts in order to test in a high-throughput way the combinations that would generate the best results. For this year, the most important part generated by our team was the pHT43 plasmid (BBa_K4103000), an improvement on (BBa_K1074001) from 2013 - this plasmid acts as a shuttle vector between E. coli and B. subtilis, and allows the efficient production and secretion of our protein-of-interest, with our team focusing efforts to use this vector for the production of Pfu-Sso7d Polymerase (BBa_K4103011) codon optimized for B. Subtilis using the software created by our team.

Basic Parts

Part Name Type Description Length (bp)
http://parts.igem.org/Part:BBa_K4103000 pfu
http://parts.igem.org/Part:BBa_K4103001 Conjugation origin Transfer Origin pIP501 319
http://parts.igem.org/Part:BBa_K4103003 Resistance marker SpecR 989
http://parts.igem.org/Part:BBa_K4103004 Origin of replication EcoORI 589
http://parts.igem.org/Part:BBa_K4103005 Origin of transfer OriTBsub 378
http://parts.igem.org/Part:BBa_K4103007 Resistance marker catA9 939
http://parts.igem.org/Part:BBa_K4103008 Insulator HDV-1 (3' Ribozyme Insulator) 85
http://parts.igem.org/Part:BBa_K4103009
http://parts.igem.org/Part:BBa_K4103016 OriTBsub
http://parts.igem.org/Part:BBa_K4103017 Terminator E. coli terminator 54
http://parts.igem.org/Part:BBa_K4103018 Promoter kanR promoter 101
http://parts.igem.org/Part:BBa_K4103019 Terminator kanR terminator 48
http://parts.igem.org/Part:BBa_K4103020 Coding kanR cds 816
http://parts.igem.org/Part:BBa_K4103021 Coding lacI cds 1083
http://parts.igem.org/Part:BBa_K4103022
http://parts.igem.org/Part:BBa_K4103023 Operator Lac operator 25
http://parts.igem.org/Part:BBa_K4103024 Insulator RiboJ54 Insulator 75
http://parts.igem.org/Part:BBa_K4103025
http://parts.igem.org/Part:BBa_K4103026 Coding Pfu (DNA polymerase) #4 2322
http://parts.igem.org/Part:BBa_K4103027 Coding Sso-7d #4 189
http://parts.igem.org/Part:BBa_K4103028 Linker TEV linker site #4 21
http://parts.igem.org/Part:BBa_K4103029 Coding fuGFP #4 732
http://parts.igem.org/Part:BBa_K4103030 Tag 10xHisTag #4 30
http://parts.igem.org/Part:BBa_K4103031 Tag R5 Silica binding purification tag #4 57
http://parts.igem.org/Part:BBa_K4103032 Coding Pfu (DNA polymerase) #2 2322
http://parts.igem.org/Part:BBa_K4103033 Coding Sso-7d #2 189
http://parts.igem.org/Part:BBa_K4103034 Linker TEV linker site 21
http://parts.igem.org/Part:BBa_K4103035 Coding fuGFP 732
http://parts.igem.org/Part:BBa_K4103036 Purification Tag 10xHisTag 30
http://parts.igem.org/Part:BBa_K4103037 Purification Tag R5 Silica binding purification tag 57
http://parts.igem.org/Part:BBa_K4103038 Coding ccdb 335
http://parts.igem.org/Part:BBa_K4103039 Homology Arm lacA 3' homology 408
http://parts.igem.org/Part:BBa_K4103040 Homology Arm amyE 5' homology 521
http://parts.igem.org/Part:BBa_K4103041 Homology Arm amyE 3' homology 498
http://parts.igem.org/Part:BBa_K4103042 Homology Arm thrC 5' homology 501
http://parts.igem.org/Part:BBa_K4103043 Homology Arm thrC 3' homology 429
http://parts.igem.org/Part:BBa_K4103044/ Homology Arm lacA 5' homology arm 602
http://parts.igem.org/Part:BBa_K4103045 Secretion Tag AmyQ 93

Composite Parts

Part Name Type Description Length (bp)
http://parts.igem.org/Part:BBa_K4103000 pfu
http://parts.igem.org/Part:BBa_K4103001 Conjugation origin Transfer Origin pIP501 319
http://parts.igem.org/Part:BBa_K4103003 Resistance marker SpecR 989
http://parts.igem.org/Part:BBa_K4103004 Origin of replication EcoORI 589
http://parts.igem.org/Part:BBa_K4103005 Origin of transfer OriTBsub 378
http://parts.igem.org/Part:BBa_K4103007 Resistance marker catA9 939
http://parts.igem.org/Part:BBa_K4103008 Insulator HDV-1 (3' Ribozyme Insulator) 85
http://parts.igem.org/Part:BBa_K4103009
http://parts.igem.org/Part:BBa_K4103016 OriTBsub
http://parts.igem.org/Part:BBa_K4103017 Terminator E. coli terminator 54
http://parts.igem.org/Part:BBa_K4103018 Promoter kanR promoter 101
http://parts.igem.org/Part:BBa_K4103019 Terminator kanR terminator 48
http://parts.igem.org/Part:BBa_K4103020 Coding kanR cds 816
http://parts.igem.org/Part:BBa_K4103021 Coding lacI cds 1083
http://parts.igem.org/Part:BBa_K4103022
http://parts.igem.org/Part:BBa_K4103023 Operator Lac operator 25
http://parts.igem.org/Part:BBa_K4103024 Insulator RiboJ54 Insulator 75
http://parts.igem.org/Part:BBa_K4103025
http://parts.igem.org/Part:BBa_K4103026 Coding Pfu (DNA polymerase) #4 2322
http://parts.igem.org/Part:BBa_K4103027 Coding Sso-7d #4 189
http://parts.igem.org/Part:BBa_K4103028 Linker TEV linker site #4 21
http://parts.igem.org/Part:BBa_K4103029 Coding fuGFP #4 732
http://parts.igem.org/Part:BBa_K4103030 Tag 10xHisTag #4 30
http://parts.igem.org/Part:BBa_K4103031 Tag R5 Silica binding purification tag #4 57
http://parts.igem.org/Part:BBa_K4103032 Coding Pfu (DNA polymerase) #2 2322
http://parts.igem.org/Part:BBa_K4103033 Coding Sso-7d #2 189
http://parts.igem.org/Part:BBa_K4103034 Linker TEV linker site 21
http://parts.igem.org/Part:BBa_K4103035 Coding fuGFP 732
http://parts.igem.org/Part:BBa_K4103036 Purification Tag 10xHisTag 30
http://parts.igem.org/Part:BBa_K4103037 Purification Tag R5 Silica binding purification tag 57
http://parts.igem.org/Part:BBa_K4103038 Coding ccdb 335
http://parts.igem.org/Part:BBa_K4103039 Homology Arm lacA 3' homology 408
http://parts.igem.org/Part:BBa_K4103040 Homology Arm amyE 5' homology 521
http://parts.igem.org/Part:BBa_K4103041 Homology Arm amyE 3' homology 498
http://parts.igem.org/Part:BBa_K4103042 Homology Arm thrC 5' homology 501
http://parts.igem.org/Part:BBa_K4103043 Homology Arm thrC 3' homology 429
http://parts.igem.org/Part:BBa_K4103044/ Homology Arm lacA 5' homology arm 602
http://parts.igem.org/Part:BBa_K4103045 Secretion Tag AmyQ 93