| − | <html lang="en"><head><meta charset="utf-8"/><meta content="width=device-width,initial-scale=1" name="viewport"/><title>Results | UIUCiGEM</title><script src="https://2020.igem.org/common/MathJax-2.5-latest/MathJax.js?config=TeX-AMS-MML_HTMLorMML"></script><link href="https://2021.igem.org/Template:UIUC_Illinois/css/contentCSS?action=raw&ctype=text/css" rel="stylesheet"/></head><body><nav class="navbar navbar-expand-xl fixed-top"><div class="container d-flex justify-content-between"><a class="navbar-brand d-lg-inline-block" href="https://2021.igem.org/Team:UIUC_Illinois"><span>iGEM </span>UIUC</a><button aria-controls="navbarNav" aria-expanded="false" aria-label="Toggle navigation" class="navbar-toggler" data-target="#navbarNav" data-toggle="collapse" type="button"><span class="navbar-toggler-icon"></span></button><div class="collapse navbar-collapse" id="navbarNav"><ul class="navbar-nav ml-auto"><li class="nav-item"><a class="nav-link" 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class="far fa-moon"></i></div></div></nav><header class="d-flex justify-content-center align-items-center"><div class="container"><h1>Results</h1><p class="lead pl-1">A Results page for the theme.</p><hr class="my-4"/></div></header><main><div class="container"><div class="row"><div class="sidebar col-lg-3"><div class="nav" id="contents"><h5>Contents</h5><ul></ul></div></div><div class="content col-lg-9"><article><h1>Drylab</h1><h1>Wetlab</h1><h2>Level 2 Heading</h2><h3>Level 3 Heading</h3><h4>Level 4 Heading</h4><h1>Emphasis</h1><p>This is regular text.</p><p><strong>This is bold text.</strong> <strong>This is also bold text.</strong></p><p><em>This is italic text.</em> _This is also italix text.*</p><p><em><strong>This text is bold and italic both.</strong></em> <em><strong>This text is bold and italic both.</strong></em></p><p><s>Strikethrough</s></p><h1>Lists</h1><ol><li>This is item one.</li><li>This is item two.</li><li>It's okay to number every item as 1.<ul><li>This is a nested list.</li><li>Use asterisk for an unordered list,</li><li>Further nesting?</li></ul><ul><li>Plus sign also works.</li></ul><ul><li>And so does minus.</li></ul></li><li><s>Birds aren't real.</s></li></ol><h1>Links</h1><p><a href="https://google.com">Google</a></p><p><a href="https://2021.igem.org/Team:UIUC_Illinois/Results/Link-goes-in-parenthesis">Text goes in square brackets</a></p><h1>Blockquote</h1><blockquote><p>This is a blockquote. This continues in the same paragraph.</p><p>To change the paragraph, you have to leave a line.</p></blockquote><blockquote><p>This is another blockquote.</p><p><strong>Pranav Ballaney, 2020</strong></p></blockquote><h1>Images</h1><p>This is a regular paragraph.</p><h4>This is a level 4 heading.</h4><p><a href="google.com">This is a link</a></p><div class="image"><img alt="Caption" src="https://static.igem.org/mediawiki/2021/f/f0/T--UIUC_Illinois--img--Description--josh-withers.jpg" style="width: 100%"/><p>Figure 1: Caption</p></div><p>Back to regular text.</p><h1>Tables</h1><p>This is a regular paragraph, which precedes the table. When you want to insert a table, indent one level back and specify the plugin. Then indent inside again and start writing the table.</p><table><caption id="table1captiongoeshere">Table 1: Caption goes here.</caption><thead><tr><th>This is</th><th>the table</th><th>header row</th></tr></thead><tbody><tr><td>1</td><td>2</td><td>3</td></tr><tr><td>4</td><td>5</td><td>6</td></tr></tbody></table><p>And when you're done, go back to the regular markdown filter.</p><p><strong>Example 2:</strong></p><table><thead><tr><th style="text-align:left">Left aligned</th><th style="text-align:center">Center aligned</th><th style="text-align:right">Right aligned</th></tr></thead><tbody><tr><td style="text-align:left">1</td><td style="text-align:center">2</td><td style="text-align:right">3</td></tr><tr><td style="text-align:left">4</td><td style="text-align:center">5</td><td style="text-align:right">6</td></tr></tbody></table><p><strong>Example 3:</strong></p><table><caption id="table3captiongoeshere">Table 3: Caption goes here.</caption><thead><tr><th></th><th colspan="2" style="text-align:center">Grouping</th></tr><tr><th>First Header</th><th style="text-align:center">Second Header</th><th style="text-align:right">Third Header</th></tr></thead><tbody><tr><td>Content</td><td colspan="2" style="text-align:center"><em>Long Cell</em></td></tr><tr><td>Content</td><td style="text-align:center"><strong>Cell</strong></td><td style="text-align:right">Cell</td></tr><tr><td>New section</td><td style="text-align:center">More</td><td style="text-align:right"><a href="https://google.com">Data</a></td></tr><tr><td>And more</td><td colspan="2" style="text-align:center">With an escaped '|'</td></tr></tbody></table><h1>Definitions</h1><p><dfn>Term ~ Definition</dfn></p><p>This can come <dfn>anywhere in ~ the text</dfn>.</p><h1>MathJax</h1><p>$$\int_{0}^{\infty} e^{-x^2} dx = \frac{\sqrt{\pi}}{2}$$</p><p>$$-\frac{\hbar^2}{2m}\nabla^2\psi + V\psi = E\psi$$</p><p>$$i\hbar\gamma^\mu\partial_\mu\psi = mc\psi$$</p><p>$$y_k = \frac{1}{\sqrt{N}}\sum_{n=0}^{N-1}x_n\omega_N^{kn}$$</p><h1>Citations</h1><p>In text citation for a research article with a DOI. <a href="#citation2">Rosano et al., 2019</a></p><p>In text citation for another research article with a DOI. <a href="#citation1">Allen & Sheridan, 2015</a></p><p>In text citation for a book with no DOI. <a href="#citation3">Ingalls, 2013</a></p><p>In text citation for a website with institutional author. <a href="#citation4">TNAU Agritech Portal, n.d.</a></p><p>In text citation for a website with an author. <a href="#citation5">Author, n.d.</a></p></article><article id="references"><h1>References</h1><ol><li id="citation1"><p class="author">Allen, M. J., & Sheridan, S. C. (2015).</p><cite>Mortality risks during extreme temperature events (ETEs) using a distributed lag non-linear model.</cite><p><span class="journalTitle">International Journal of Biometeorology</span> <span class="journalInfo">62(1), 57-67.</span></p><a class="in-text" href="https://doi.org/10.1007/s00484-015-1117-4" rel="noopener" target="_blank">CrossRef</a><a class="in-text" href="https://scholar.google.com/scholar?q=Mortality risks during extreme temperature events (ETEs) using a distributed lag non-linear model." rel="noopener" target="_blank">Google Scholar</a><a class="in-text" href="#intext1">Back to text</a></li><li id="citation2"><p class="author">Rosano, A., Bella, A., Gesualdo, F., Acampora, A., Pezzotti, P., Marchetti, S., ... & Rizzo, C. (2019).</p><cite>Investigating the impact of influenza on excess mortality in all ages in Italy during recent seasons (2013/14-2016/17 seasons).</cite><p><span class="journalTitle">International Journal of Infectious Diseases</span> <span class="journalInfo">88, 127-134.</span></p><a class="in-text" href="https://doi.org/10.1016/j.ijid.2019.08.003" rel="noopener" target="_blank">CrossRef</a><a class="in-text" href="https://scholar.google.com/scholar?q=Investigating the impact of influenza on excess mortality in all ages in Italy during recent seasons (2013/14-2016/17 seasons)." rel="noopener" target="_blank">Google Scholar</a><a class="in-text" href="#intext2">Back to text</a></li><li id="citation3"><p class="author">Ingalls, B. P. (2013).</p><cite>Mathematical modeling in systems biology: An introduction.</cite><p><span class="details">MIT Press.</span></p><a class="in-text" href="https://books.google.co.in/books?id=OYr6AQAAQBAJ" rel="noopener" target="_blank">Google Books</a><a class="in-text" href="#intext3">Back to text</a></li><li id="citation4"><p class="author"></p><cite>Agriculture: Crop production: Sugarcane. TNAU Agritech Portal.</cite><p><span class="details">(March 15, 2019). Retrieved on June 22, 2020. from </span><a class="in-text" href="https://google.com" rel="noopener" target="_blank">https://google.com</a></p><a class="in-text" href="#intext4">Back to text</a></li><li id="citation5"><p class="author">Author Name. (n.d.).</p><cite>Agriculture: Crop production: Sugarcane. TNAU Agritech Portal.</cite><p><span class="details">Retrieved on June 22, 2020. from </span><a class="in-text" href="https://google.com" rel="noopener" target="_blank">https://google.com</a></p><a class="in-text" href="#intext5">Back to text</a></li></ol></article></div></div></div></main><footer><div class="container"><p>Sample template built using the iGEM Wiki Starter Pack by BITS Goa.</p><p>Code released under the MIT license.</p><p>Based on <a href="https://getbootstrap.com">Bootstrap</a> and themes <a href="https://bootswatch.com/flatly/">Flatly</a> and <a href="https://bootswatch.com/darkly/">Darkly</a> from <a href="https://bootswatch.com/">Bootswatch</a>.</p><p>Some content from the <a href="https://2020.igem.org/Team:Example">iGEM Example Wiki</a>. Images from <a href="https://unsplash.com">Unsplash</a>. 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class="far fa-moon"></i></div></div></nav><header class="d-flex justify-content-center align-items-center"><div class="container"><h1>Results</h1><p class="lead pl-1">Wetlab Data</p><hr class="my-4"/></div></header><main><div class="container"><div class="row"><div class="sidebar col-lg-3"><div class="nav" id="contents"><h5>Contents</h5><ul></ul></div></div><div class="content col-lg-9"><article><h1>Nanodrop Kinetic Assay Results</h1><p>Due to our complications in our purification of PETase, we decided to begin our testing on our crude unpurified soluble sample. From our literature review, we decided to use a Nanodrop method for evaluating the enzymatic degradation kinetics of PET films as described by Zhong-Johnson et al. Specifically, we utilized a “bulk absorbance method” in which all degradation products contribute to the absorbance.</p><p>We initially took an assay of our crude soluble sample as we were still in the process of conducting FPLC and purifying the wild type PETase. We used a Thermomixer set to 200 rpm and incubated our samples at a temperature of 40 °C. From this assay, we were able to determine the ideal quantities of protein to use for testing the enzymatic degradation kinetics of PETase. Additionally, after leaving these samples at room temperature</p><h1>SDS Page</h1><h1>Wet Lab Data Induction Troubleshooting Assay</h1><p>In this assay we had two tubes of BL21 E. coli cells with our plasmid of interest in them. Both tubes were grown in LB overnight; however, the tube to the left was induced with IPTG beforehand and the tube to the right was not. The uninduced tube showed a higher visual concentration of cells and led us to conclude that that induction of the PETase protein hindered the growth of the BL21.</p><h1>Wet Lab Data Crude PET Degradation Visual Assay</h1><p>In this assay we set up microcentrifuge tubes with various concentrations of crude C41 cell extract with PETase, ¼ hole punch of pet film, and reaction buffer. We let the tubes sit overnight at room temperature and observed the results the following day.</p></article></div></div></div></main><footer><div class="container"><p>Sample template built using the iGEM Wiki Starter Pack by BITS Goa.</p><p>Code released under the MIT license.</p><p>Based on <a href="https://getbootstrap.com">Bootstrap</a> and themes <a href="https://bootswatch.com/flatly/">Flatly</a> and <a href="https://bootswatch.com/darkly/">Darkly</a> from <a href="https://bootswatch.com/">Bootswatch</a>.</p><p>Some content from the <a href="https://2020.igem.org/Team:Example">iGEM Example Wiki</a>. Images from <a href="https://unsplash.com">Unsplash</a>. Web fonts from <a href="https://fonts.google.com">Google</a>.</p></div></footer><script src="https://2021.igem.org/Template:UIUC_Illinois/content-bundleJS?action=raw&ctype=text/javascript"></script></body></html> |
