The three sequences are the computationally generated and tested in the wetlab. Team Toronto generously provided the estimated thermostability and catalytic activity of our sequences based on their model.
| Name | Description | Tm(Celcius) | catalytic activity |
|---|---|---|---|
| BBa_K3713001 | Variant 1 of PETase candidates | 82.9 | -0.60 |
| BBa_K3713002 | Variant 2 of PETase candidates | 81.5 | 1.58 |
| BBa_K3713003 | Variant 3 of PETase candidates | 81.9 | 0.50 |
For the thermostability measure, Tm, the melting temperature of protein sequence is used. Thermostability model is more accurate than the catlytic activity model. Catalytic activity values are the relative ranks, 1 being the worst, 2 being the best and 3 for okay.