The three sequences are the computationally generated and tested in the wetlab. Team Toronto generously provided the estimated thermostability and catalytic activity of our sequences based on their model.
|BBa_K3713001||Variant 1 of PETase candidates||82.9||-0.60|
|BBa_K3713002||Variant 2 of PETase candidates||81.5||1.58|
|BBa_K3713003||Variant 3 of PETase candidates||81.9||0.50|
For the thermostability measure, Tm, the melting temperature of protein sequence is used. Thermostability model is more accurate than the catlytic activity model. Catalytic activity values are the relative ranks, 1 being the worst, 2 being the best and 3 for okay.