Difference between revisions of "Team:Open Science Global/Part Collection"

 
Line 4: Line 4:
 
<html>
 
<html>
 
<style>
 
<style>
−
    @import url('https://fonts.googleapis.com/css2?family=Roboto&display=swap');
+
@import url('https://fonts.googleapis.com/css2?family=Roboto&display=swap');
−
 
+
−
   
+
 
</style>
 
</style>
 
<!-------------------- HTML CONTENT -------------------------------->
 
<!-------------------- HTML CONTENT -------------------------------->
  
 
<body>
 
<body>
−
    <div class="top-banner">
+
  <div class="top-banner"></div>
 +
  <div class="Subtitle">
 +
    <div class="Title">Part Collection</div>
 +
  </div>
 +
  <div class="container">
 +
    <div class="nav-menu">
 +
      <div class="submenu-title">Part Collection</div>
 +
      <div class="submenu-content">
 +
        <a href="#Overview">Overview</a>
 +
        <a href="#Basic Parts">Basic Parts</a>
 +
        <a href="#Composite Parts">Composite Parts</a>
 +
      </div>
 
     </div>
 
     </div>
 +
    <div class="text-container">
 +
      <div class=title-container><h2 id="Overview">Overview</h2></div>
 +
      <div class="text">
 +
        <p>
 +
          The main goal of our project is to make a frugal biofoundry capable of producing high quantities of enzymes and other proteins of interest. To do that, our team designed a library of parts in order to test in a high-throughput way the combinations that would generate the best results. For this year, the most important part generated by our team was the pHT43 plasmid (BBa_K4103000), an improvement on (BBa_K1074001) from 2013 - this plasmid acts as a shuttle vector between E. coli and B. subtilis, and allows the efficient production and secretion of our protein-of-interest, with our team focusing efforts to use this vector for the production of Pfu-Sso7d Polymerase (BBa_K4103011) codon optimized for B. Subtilis using the software created by our team.
 +
        </p>
 +
      </div>
 +
      <div class="title-container"><h2 id="Basic Parts">Basic Parts</h2></div>
 +
      <style type="text/css">
 +
        .tg {
 +
                              border-collapse:    collapse;
 +
                              border-color:      #ccc;
 +
                              border-spacing:    0;
 +
        }
  
−
    <div class="Subtitle">
+
        .tg td {
−
         <div class="Title">Part Collection</div>
+
                              background-color:  #fff;
−
    </div>
+
                              border-color:      #ccc;
−
    <div class="container">
+
                              border-style:      solid;
 +
                              border-width:      1px;
 +
                              color:              #333;
 +
                              font-family:        Roboto;
 +
                              font-size:          14px;
 +
                              overflow:          hidden;
 +
                              padding:            10px 5px;
 +
                              word-break:         normal;
 +
        }
 +
 
 +
        .tg th {
 +
                              background-color:  #f0f0f0;
 +
                              border-color:      #ccc;
 +
                              border-style:      solid;
 +
                              border-width:      1px;
 +
                              color:              #333;
 +
                              font-family:        Roboto;
 +
                              font-size:          14px;
 +
                              font-weight:        normal;
 +
                              overflow:          hidden;
 +
                              padding:            10px 5px;
 +
                              word-break:        normal;
 +
        }
  
−
         <div class="subsite-container">
+
         .tg .tg-3rmm {
−
            <div class = "submenu-title">Results</div>
+
                              background-color:  #ffffff;
−
            <div class = index-box>
+
                              border-color:       inherit;
−
                <a href="https://2021.igem.org/Team:Open_Science_Global/Wetware">Wetware</a>
+
                              color:             #15C;
−
                <a href="https://2021.igem.org/Team:Open_Science_Global/Parts">Parts</a>
+
                              text-align:         left;
−
                <a href="https://2021.igem.org/Team:Open_Science_Global/Part_Collection">Parts Collection</a>
+
                              text-decoration:   underline;
−
                <a href="https://2021.igem.org/Team:Open_Science_Global/Notebook">Notebook</a>
+
                              vertical-align:     top;
−
                <a href="https://2021.igem.org/Team:Open_Science_Global/Safety">Safety</a>
+
         }
−
            </div>
+
−
         </div>
+
  
−
         <div class="nav-menu">
+
         .tg .tg-lp5f {
−
            <div class="submenu-title">Part Collection</div>
+
                              background-color:  #efefef;
 +
                              border-color:      inherit;
 +
                              font-family:        "Roboto Black", Gadget, Roboto !important;
 +
                              text-align:        center;
 +
                              vertical-align:    top;
 +
        }
  
−
            <div class="submenu-content">
+
        .tg .tg-3xi5 {
−
                <a href="#Overview">Overview</a>
+
                              background-color:  #ffffff;
−
                <a href="#Basic Parts">Basic Parts</a>
+
                              border-color:      inherit;
−
                <a href="#Composite Parts">Composite Parts</a>
+
                              text-align:        center;
−
            </div>
+
                              vertical-align:    top;
 +
        }
  
−
         </div>
+
         .tg .tg-c6of {
 +
                              background-color:  #ffffff;
 +
                              border-color:      inherit;
 +
                              text-align:        left;
 +
                              vertical-align:    top;
 +
        }
  
−
         <div class="text-container">
+
         .tg {
−
            <div class = title-container>
+
                              align-self:         center;
−
            <h2 id="Overview">Overview</h2>
+
                              width:             80%;
−
            </div>
+
        }
−
            <div class="text">
+
      </style>
−
                <p>
+
          <table class="tg">
−
                    The main goal of our project is to make a frugal biofoundry capable of producing high quantities of enzymes and other proteins of interest. To do that, our team designed a library of parts in order to test in a high-throughput way the combinations that would generate the best results. For this year, the most important part generated by our team was the pHT43 plasmid (BBa_K4103000), an improvement on (BBa_K1074001) from 2013 - this plasmid acts as a shuttle vector between E. coli and B. subtilis, and allows the efficient production and secretion of our protein-of-interest, with our team focusing efforts to use this vector for the production of Pfu-Sso7d Polymerase (BBa_K4103011) codon optimized for B. Subtilis using the software created by our team.
+
              <thead>
−
                </p>
+
−
            </div>
+
−
            <div class = "title-container">
+
−
            <h2 id="Basic Parts">Basic Parts</h2>
+
−
            </div>
+
−
            <style type="text/css">
+
−
                .tg  {border-collapse:collapse;border-color:#ccc;border-spacing:0;}
+
−
                .tg td{background-color:#fff;border-color:#ccc;border-style:solid;border-width:1px;color:#333;
+
−
                  font-family:Roboto, Roboto;font-size:14px;overflow:hidden;padding:10px 5px;word-break:normal;}
+
−
                .tg th{background-color:#f0f0f0;border-color:#ccc;border-style:solid;border-width:1px;color:#333;
+
−
                  font-family:Roboto, Roboto;font-size:14px;font-weight:normal;overflow:hidden;padding:10px 5px;word-break:normal;}
+
−
                .tg .tg-3rmm{background-color:#ffffff;border-color:inherit;color:#15C;text-align:left;text-decoration:underline;vertical-align:top}
+
−
                .tg .tg-lp5f{background-color:#efefef;border-color:inherit;font-family:"Roboto Black", Gadget, Roboto !important;;
+
−
                  text-align:center;vertical-align:top}
+
−
                .tg .tg-3xi5{background-color:#ffffff;border-color:inherit;text-align:center;vertical-align:top}
+
−
                .tg .tg-c6of{background-color:#ffffff;border-color:inherit;text-align:left;vertical-align:top}
+
−
                .tg{
+
−
                    align-self: center;
+
−
                    width: 80%;
+
−
                }
+
−
                </style>
+
−
                <table class="tg">
+
−
                <thead>
+
 
                   <tr>
 
                   <tr>
−
                    <th class="tg-lp5f"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Part Name</span></th>
+
                      <th class="tg-lp5f"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Part Name</span></th>
−
                    <th class="tg-lp5f"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Type</span></th>
+
                      <th class="tg-lp5f"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Type</span></th>
−
                    <th class="tg-lp5f"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Description</span></th>
+
                      <th class="tg-lp5f"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Description</span></th>
−
                    <th class="tg-lp5f"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Length (bp)</span></th>
+
                      <th class="tg-lp5f"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Length (bp)</span></th>
 
                   </tr>
 
                   </tr>
−
                </thead>
+
              </thead>
−
                <tbody>
+
              <tbody>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103000" target="_blank" rel="noopener noreferrer"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103000</span></a></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103000" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103000</span></a></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">pfu</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">pfu</span></td>
−
                    <td class="tg-c6of"></td>
+
                      <td class="tg-c6of"></td>
−
                    <td class="tg-c6of"></td>
+
                      <td class="tg-c6of"></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103001" target="_blank" rel="noopener noreferrer"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103001</span></a></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103001" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103001</span></a></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Conjugation origin</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Conjugation origin</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Transfer Origin pIP501</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Transfer Origin pIP501</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">319</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">319</span></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103003" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103003</span></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103003" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103003</span></a></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Resistance marker</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Resistance marker</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">SpecR</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">SpecR</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">989</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">989</span></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103004" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103004</span></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103004" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103004</span></a></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Origin of replication</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Origin of replication</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">EcoORI</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">EcoORI</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">589</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">589</span></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103005" target="_blank" rel="noopener noreferrer"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103005</span></a></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103005" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103005</span></a></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Origin of transfer</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Origin of transfer</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">OriTBsub</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">OriTBsub</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">378</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">378</span></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103007" target="_blank" rel="noopener noreferrer"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103007</span></a></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103007" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103007</span></a></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Resistance marker</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Resistance marker</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">catA9</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">catA9</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">939</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">939</span></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103008" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103008</span></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103008" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103008</span></a></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Insulator</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Insulator</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">HDV-1 (3' Ribozyme Insulator)</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">HDV-1 (3' Ribozyme Insulator)</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">85</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">85</span></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103009" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103009</span></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103009" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103009</span></a></td>
−
                    <td class="tg-c6of"></td>
+
                      <td class="tg-c6of"></td>
−
                    <td class="tg-c6of"></td>
+
                      <td class="tg-c6of"></td>
−
                    <td class="tg-c6of"></td>
+
                      <td class="tg-c6of"></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103016" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103016</span></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103016" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103016</span></a></td>
−
                    <td class="tg-c6of"></td>
+
                      <td class="tg-c6of"></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">OriTBsub</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">OriTBsub</span></td>
−
                    <td class="tg-c6of"></td>
+
                      <td class="tg-c6of"></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103017" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103017</span></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103017" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103017</span></a></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Terminator</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Terminator</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">E. coli terminator</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">E. coli terminator</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">54</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">54</span></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103018" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103018</span></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103018" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103018</span></a></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Promoter</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Promoter</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">kanR promoter</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">kanR promoter</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">101</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">101</span></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103019" target="_blank" rel="noopener noreferrer"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103019</span></a></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103019" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103019</span></a></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Terminator</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Terminator</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">kanR terminator</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">kanR terminator</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">48</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">48</span></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103020" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103020</span></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103020" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103020</span></a></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">kanR cds</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">kanR cds</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">816</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">816</span></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103021" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103021</span></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103021" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103021</span></a></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">lacI cds</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">lacI cds</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">1083</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">1083</span></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103022" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103022</span></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103022" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103022</span></a></td>
−
                    <td class="tg-c6of"></td>
+
                      <td class="tg-c6of"></td>
−
                    <td class="tg-c6of"></td>
+
                      <td class="tg-c6of"></td>
−
                    <td class="tg-c6of"></td>
+
                      <td class="tg-c6of"></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103023" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103023</span></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103023" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103023</span></a></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Operator</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Operator</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Lac operator</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Lac operator</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">25</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">25</span></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103024" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103024</span></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103024" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103024</span></a></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Insulator</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Insulator</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">RiboJ54 Insulator</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">RiboJ54 Insulator</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">75</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">75</span></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103025" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103025</span></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103025" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103025</span></a></td>
−
                    <td class="tg-c6of"></td>
+
                      <td class="tg-c6of"></td>
−
                    <td class="tg-c6of"></td>
+
                      <td class="tg-c6of"></td>
−
                    <td class="tg-c6of"></td>
+
                      <td class="tg-c6of"></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103026" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103026</span></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103026" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103026</span></a></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Pfu (DNA polymerase) #4</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Pfu (DNA polymerase) #4</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">2322</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">2322</span></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103027" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103027</span></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103027" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103027</span></a></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Sso-7d #4</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Sso-7d #4</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">189</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">189</span></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103028" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103028</span></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103028" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103028</span></a></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Linker</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Linker</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">TEV linker site #4</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">TEV linker site #4</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">21</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">21</span></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103029" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103029</span></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103029" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103029</span></a></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">fuGFP #4</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">fuGFP #4</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">732</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">732</span></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103030" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103030</span></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103030" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103030</span></a></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Tag</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Tag</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">10xHisTag #4</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">10xHisTag #4</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">30</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">30</span></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103031" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103031</span></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103031" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103031</span></a></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Tag</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Tag</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">R5 Silica binding purification tag #4</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">R5 Silica binding purification tag #4</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">57</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">57</span></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103032" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103032</span></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103032" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103032</span></a></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Pfu (DNA polymerase) #2</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Pfu (DNA polymerase) #2</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">2322</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">2322</span></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103033" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103033</span></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103033" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103033</span></a></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Sso-7d #2</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Sso-7d #2</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">189</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">189</span></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103034" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103034</span></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103034" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103034</span></a></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Linker</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Linker</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">TEV linker site</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">TEV linker site</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">21</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">21</span></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103035" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103035</span></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103035" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103035</span></a></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">fuGFP</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">fuGFP</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">732</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">732</span></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103036" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103036</span></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103036" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103036</span></a></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Purification Tag</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Purification Tag</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">10xHisTag</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">10xHisTag</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">30</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">30</span></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103037" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103037</span></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103037" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103037</span></a></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Purification Tag</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Purification Tag</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">R5 Silica binding purification tag</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">R5 Silica binding purification tag</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">57</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">57</span></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103038" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103038</span></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103038" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103038</span></a></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">ccdb</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">ccdb</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">335</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">335</span></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103039" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103039</span></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103039" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103039</span></a></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">lacA 3' homology</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">lacA 3' homology</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">408</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">408</span></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103040" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103040</span></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103040" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103040</span></a></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">amyE 5' homology</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">amyE 5' homology</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">521</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">521</span></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103041" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103041</span></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103041" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103041</span></a></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">amyE 3' homology</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">amyE 3' homology</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">498</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">498</span></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103042" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103042</span></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103042" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103042</span></a></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">thrC 5' homology</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">thrC 5' homology</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">501</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">501</span></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103043" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103043</span></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103043" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103043</span></a></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">thrC 3' homology</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">thrC 3' homology</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">429</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">429</span></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103044" target="_blank" rel="noopener noreferrer"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103044</span></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">/</span></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103044" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103044</span></a></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">lacA 5' homology arm</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">lacA 5' homology arm</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">602</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">602</span></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103045" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103045</span></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103045" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103045</span></a></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Secretion Tag</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Secretion Tag</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">AmyQ</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">AmyQ</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">93</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">93</span></td>
 
                   </tr>
 
                   </tr>
−
                </tbody>
+
              </tbody>
−
                </table>
+
          </table>
−
           
+
          <div class="title-container">
 +
              <h2 id="Composite Parts">Composite Parts</h2>
 +
          </div>
 +
          <style type="text/css">
 +
          .tg {
 +
              border-collapse: collapse;
 +
              border-color: #ccc;
 +
              border-spacing: 0;
 +
          }
  
−
            <div class = "title-container">   
+
          .tg td {
−
            <h2 id="Composite Parts">Composite Parts</h2>
+
              background-color: #fff;
−
            </div>
+
              border-color: #ccc;
−
            <style type="text/css">
+
              border-style: solid;
−
                .tg {border-collapse:collapse;border-color:#ccc;border-spacing:0;}
+
              border-width: 1px;
−
                .tg td{background-color:#fff;border-color:#ccc;border-style:solid;border-width:1px;color:#333;
+
              color: #333;
−
                  font-family:Roboto, Roboto;font-size:14px;overflow:hidden;padding:10px 5px;word-break:normal;}
+
              font-family: Roboto, Roboto;
−
                .tg th{background-color:#f0f0f0;border-color:#ccc;border-style:solid;border-width:1px;color:#333;
+
              font-size: 14px;
−
                  font-family:Roboto, Roboto;font-size:14px;font-weight:normal;overflow:hidden;padding:10px 5px;word-break:normal;}
+
              overflow: hidden;
−
                .tg .tg-3rmm{background-color:#ffffff;border-color:inherit;color:#15C;text-align:left;text-decoration:underline;vertical-align:top}
+
              padding: 10px 5px;
−
                .tg .tg-lp5f{background-color:#efefef;border-color:inherit;font-family:"Roboto Black", Gadget, Roboto !important;;
+
              word-break: normal;
−
                  text-align:center;vertical-align:top}
+
          }
−
                .tg .tg-3xi5{background-color:#ffffff;border-color:inherit;text-align:center;vertical-align:top}
+
−
                .tg .tg-c6of{background-color:#ffffff;border-color:inherit;text-align:left;vertical-align:top}
+
−
                .tg{
+
−
                    align-self: center;
+
−
                    width: 80%;
+
−
                }
+
  
−
                </style>
+
          .tg th {
−
                <table class="tg">
+
              background-color: #f0f0f0;
−
                <thead>
+
              border-color: #ccc;
 +
              border-style: solid;
 +
              border-width: 1px;
 +
              color: #333;
 +
              font-family: Roboto, Roboto;
 +
              font-size: 14px;
 +
              font-weight: normal;
 +
              overflow: hidden;
 +
              padding: 10px 5px;
 +
              word-break: normal;
 +
          }
 +
 
 +
          .tg .tg-3rmm {
 +
              background-color: #ffffff;
 +
              border-color: inherit;
 +
              color: #15C;
 +
              text-align: left;
 +
              text-decoration: underline;
 +
              vertical-align: top
 +
          }
 +
 
 +
          .tg .tg-lp5f {
 +
              background-color: #efefef;
 +
              border-color: inherit;
 +
              font-family: "Roboto Black", Gadget, Roboto !important;
 +
              ;
 +
              text-align: center;
 +
              vertical-align: top
 +
          }
 +
 
 +
          .tg .tg-3xi5 {
 +
              background-color: #ffffff;
 +
              border-color: inherit;
 +
              text-align: center;
 +
              vertical-align: top
 +
          }
 +
 
 +
          .tg .tg-c6of {
 +
              background-color: #ffffff;
 +
              border-color: inherit;
 +
              text-align: left;
 +
              vertical-align: top
 +
          }
 +
 
 +
          .tg {
 +
              align-self: center;
 +
              width: 80%;
 +
          }
 +
          </style>
 +
          <table class="tg">
 +
              <thead>
 
                   <tr>
 
                   <tr>
−
                    <th class="tg-lp5f"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Part Name</span></th>
+
                      <th class="tg-lp5f"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Part Name</span></th>
−
                    <th class="tg-lp5f"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Type</span></th>
+
                      <th class="tg-lp5f"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Type</span></th>
−
                    <th class="tg-lp5f"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Description</span></th>
+
                      <th class="tg-lp5f"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Description</span></th>
−
                    <th class="tg-lp5f"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Length (bp)</span></th>
+
                      <th class="tg-lp5f"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Length (bp)</span></th>
 
                   </tr>
 
                   </tr>
−
                </thead>
+
              </thead>
−
                <tbody>
+
              <tbody>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103000" target="_blank" rel="noopener noreferrer"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103000</span></a></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103000" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103000</span></a></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">pfu</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">pfu</span></td>
−
                    <td class="tg-c6of"></td>
+
                      <td class="tg-c6of"></td>
−
                    <td class="tg-c6of"></td>
+
                      <td class="tg-c6of"></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103001" target="_blank" rel="noopener noreferrer"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103001</span></a></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103001" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103001</span></a></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Conjugation origin</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Conjugation origin</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Transfer Origin pIP501</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Transfer Origin pIP501</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">319</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">319</span></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103003" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103003</span></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103003" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103003</span></a></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Resistance marker</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Resistance marker</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">SpecR</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">SpecR</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">989</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">989</span></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103004" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103004</span></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103004" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103004</span></a></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Origin of replication</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Origin of replication</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">EcoORI</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">EcoORI</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">589</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">589</span></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103005" target="_blank" rel="noopener noreferrer"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103005</span></a></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103005" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103005</span></a></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Origin of transfer</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Origin of transfer</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">OriTBsub</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">OriTBsub</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">378</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">378</span></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103007" target="_blank" rel="noopener noreferrer"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103007</span></a></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103007" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103007</span></a></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Resistance marker</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Resistance marker</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">catA9</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">catA9</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">939</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">939</span></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103008" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103008</span></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103008" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103008</span></a></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Insulator</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Insulator</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">HDV-1 (3' Ribozyme Insulator)</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">HDV-1 (3' Ribozyme Insulator)</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">85</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">85</span></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103009" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103009</span></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103009" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103009</span></a></td>
−
                    <td class="tg-c6of"></td>
+
                      <td class="tg-c6of"></td>
−
                    <td class="tg-c6of"></td>
+
                      <td class="tg-c6of"></td>
−
                    <td class="tg-c6of"></td>
+
                      <td class="tg-c6of"></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030016" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103016</span></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030016" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103016</span></a></td>
−
                    <td class="tg-c6of"></td>
+
                      <td class="tg-c6of"></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">OriTBsub</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">OriTBsub</span></td>
−
                    <td class="tg-c6of"></td>
+
                      <td class="tg-c6of"></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030017" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103017</span></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030017" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103017</span></a></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Terminator</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Terminator</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">E. coli terminator</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">E. coli terminator</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">54</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">54</span></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030018" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103018</span></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030018" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103018</span></a></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Promoter</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Promoter</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">kanR promoter</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">kanR promoter</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">101</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">101</span></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103019" target="_blank" rel="noopener noreferrer"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103019</span></a></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103019" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103019</span></a></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Terminator</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Terminator</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">kanR terminator</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">kanR terminator</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">48</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">48</span></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030020" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103020</span></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030020" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103020</span></a></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">kanR cds</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">kanR cds</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">816</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">816</span></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030021" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103021</span></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030021" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103021</span></a></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">lacI cds</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">lacI cds</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">1083</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">1083</span></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030022" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103022</span></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030022" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103022</span></a></td>
−
                    <td class="tg-c6of"></td>
+
                      <td class="tg-c6of"></td>
−
                    <td class="tg-c6of"></td>
+
                      <td class="tg-c6of"></td>
−
                    <td class="tg-c6of"></td>
+
                      <td class="tg-c6of"></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030023" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103023</span></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030023" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103023</span></a></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Operator</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Operator</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Lac operator</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Lac operator</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">25</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">25</span></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030024" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103024</span></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030024" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103024</span></a></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Insulator</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Insulator</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">RiboJ54 Insulator</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">RiboJ54 Insulator</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">75</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">75</span></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030025" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103025</span></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030025" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103025</span></a></td>
−
                    <td class="tg-c6of"></td>
+
                      <td class="tg-c6of"></td>
−
                    <td class="tg-c6of"></td>
+
                      <td class="tg-c6of"></td>
−
                    <td class="tg-c6of"></td>
+
                      <td class="tg-c6of"></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030026" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103026</span></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030026" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103026</span></a></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Pfu (DNA polymerase) #4</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Pfu (DNA polymerase) #4</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">2322</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">2322</span></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030027" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103027</span></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030027" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103027</span></a></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Sso-7d #4</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Sso-7d #4</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">189</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">189</span></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030028" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103028</span></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030028" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103028</span></a></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Linker</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Linker</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">TEV linker site #4</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">TEV linker site #4</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">21</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">21</span></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030029" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103029</span></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030029" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103029</span></a></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">fuGFP #4</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">fuGFP #4</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">732</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">732</span></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030030" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103030</span></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030030" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103030</span></a></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Tag</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Tag</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">10xHisTag #4</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">10xHisTag #4</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">30</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">30</span></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030031" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103031</span></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030031" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103031</span></a></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Tag</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Tag</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">R5 Silica binding purification tag #4</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">R5 Silica binding purification tag #4</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">57</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">57</span></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030032" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103032</span></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030032" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103032</span></a></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Pfu (DNA polymerase) #2</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Pfu (DNA polymerase) #2</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">2322</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">2322</span></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030033" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103033</span></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030033" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103033</span></a></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Sso-7d #2</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Sso-7d #2</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">189</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">189</span></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030034" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103034</span></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030034" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103034</span></a></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Linker</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Linker</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">TEV linker site</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">TEV linker site</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">21</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">21</span></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030035" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103035</span></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030035" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103035</span></a></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">fuGFP</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">fuGFP</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">732</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">732</span></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030036" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103036</span></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030036" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103036</span></a></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Purification Tag</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Purification Tag</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">10xHisTag</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">10xHisTag</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">30</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">30</span></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030037" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103037</span></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030037" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103037</span></a></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Purification Tag</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Purification Tag</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">R5 Silica binding purification tag</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">R5 Silica binding purification tag</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">57</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">57</span></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030038" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103038</span></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030038" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103038</span></a></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">ccdb</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">ccdb</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">335</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">335</span></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030039" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103039</span></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030039" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103039</span></a></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">lacA 3' homology</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">lacA 3' homology</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">408</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">408</span></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030040" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103040</span></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030040" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103040</span></a></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">amyE 5' homology</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">amyE 5' homology</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">521</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">521</span></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030041" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103041</span></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030041" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103041</span></a></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">amyE 3' homology</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">amyE 3' homology</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">498</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">498</span></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030042" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103042</span></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030042" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103042</span></a></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">thrC 5' homology</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">thrC 5' homology</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">501</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">501</span></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030043" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103043</span></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030043" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103043</span></a></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">thrC 3' homology</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">thrC 3' homology</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">429</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">429</span></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103044" target="_blank" rel="noopener noreferrer"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103044</span></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">/</span></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103044" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103044</span></a></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">lacA 5' homology arm</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">lacA 5' homology arm</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">602</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">602</span></td>
 
                   </tr>
 
                   </tr>
 
                   <tr>
 
                   <tr>
−
                    <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K410300" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103045</span></td>
+
                      <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K410300" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103045</span></a></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Secretion Tag</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Secretion Tag</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">AmyQ</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">AmyQ</span></td>
−
                    <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">93</span></td>
+
                      <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">93</span></td>
 
                   </tr>
 
                   </tr>
−
                </tbody>
+
              </tbody>
−
                </table>
+
          </table>
−
 
+
      </div>
−
 
+
      <footer></footer>
−
 
+
  </div>
−
 
+
−
        </div>
+
−
 
+
−
 
+
−
           
+
−
 
+
−
 
+
−
        <footer>
+
−
 
+
−
        </footer>
+
−
    </div>
+
 
</body>
 
</body>
−
 
 
</html>
 
</html>

Latest revision as of 01:24, 18 December 2021

Friendzymes
Part Collection
Part Collection
Overview Basic Parts Composite Parts

Overview

The main goal of our project is to make a frugal biofoundry capable of producing high quantities of enzymes and other proteins of interest. To do that, our team designed a library of parts in order to test in a high-throughput way the combinations that would generate the best results. For this year, the most important part generated by our team was the pHT43 plasmid (BBa_K4103000), an improvement on (BBa_K1074001) from 2013 - this plasmid acts as a shuttle vector between E. coli and B. subtilis, and allows the efficient production and secretion of our protein-of-interest, with our team focusing efforts to use this vector for the production of Pfu-Sso7d Polymerase (BBa_K4103011) codon optimized for B. Subtilis using the software created by our team.

Basic Parts

Part Name Type Description Length (bp)
http://parts.igem.org/Part:BBa_K4103000 pfu
http://parts.igem.org/Part:BBa_K4103001 Conjugation origin Transfer Origin pIP501 319
http://parts.igem.org/Part:BBa_K4103003 Resistance marker SpecR 989
http://parts.igem.org/Part:BBa_K4103004 Origin of replication EcoORI 589
http://parts.igem.org/Part:BBa_K4103005 Origin of transfer OriTBsub 378
http://parts.igem.org/Part:BBa_K4103007 Resistance marker catA9 939
http://parts.igem.org/Part:BBa_K4103008 Insulator HDV-1 (3' Ribozyme Insulator) 85
http://parts.igem.org/Part:BBa_K4103009
http://parts.igem.org/Part:BBa_K4103016 OriTBsub
http://parts.igem.org/Part:BBa_K4103017 Terminator E. coli terminator 54
http://parts.igem.org/Part:BBa_K4103018 Promoter kanR promoter 101
http://parts.igem.org/Part:BBa_K4103019 Terminator kanR terminator 48
http://parts.igem.org/Part:BBa_K4103020 Coding kanR cds 816
http://parts.igem.org/Part:BBa_K4103021 Coding lacI cds 1083
http://parts.igem.org/Part:BBa_K4103022
http://parts.igem.org/Part:BBa_K4103023 Operator Lac operator 25
http://parts.igem.org/Part:BBa_K4103024 Insulator RiboJ54 Insulator 75
http://parts.igem.org/Part:BBa_K4103025
http://parts.igem.org/Part:BBa_K4103026 Coding Pfu (DNA polymerase) #4 2322
http://parts.igem.org/Part:BBa_K4103027 Coding Sso-7d #4 189
http://parts.igem.org/Part:BBa_K4103028 Linker TEV linker site #4 21
http://parts.igem.org/Part:BBa_K4103029 Coding fuGFP #4 732
http://parts.igem.org/Part:BBa_K4103030 Tag 10xHisTag #4 30
http://parts.igem.org/Part:BBa_K4103031 Tag R5 Silica binding purification tag #4 57
http://parts.igem.org/Part:BBa_K4103032 Coding Pfu (DNA polymerase) #2 2322
http://parts.igem.org/Part:BBa_K4103033 Coding Sso-7d #2 189
http://parts.igem.org/Part:BBa_K4103034 Linker TEV linker site 21
http://parts.igem.org/Part:BBa_K4103035 Coding fuGFP 732
http://parts.igem.org/Part:BBa_K4103036 Purification Tag 10xHisTag 30
http://parts.igem.org/Part:BBa_K4103037 Purification Tag R5 Silica binding purification tag 57
http://parts.igem.org/Part:BBa_K4103038 Coding ccdb 335
http://parts.igem.org/Part:BBa_K4103039 Homology Arm lacA 3' homology 408
http://parts.igem.org/Part:BBa_K4103040 Homology Arm amyE 5' homology 521
http://parts.igem.org/Part:BBa_K4103041 Homology Arm amyE 3' homology 498
http://parts.igem.org/Part:BBa_K4103042 Homology Arm thrC 5' homology 501
http://parts.igem.org/Part:BBa_K4103043 Homology Arm thrC 3' homology 429
http://parts.igem.org/Part:BBa_K4103044 Homology Arm lacA 5' homology arm 602
http://parts.igem.org/Part:BBa_K4103045 Secretion Tag AmyQ 93

Composite Parts

Part Name Type Description Length (bp)
http://parts.igem.org/Part:BBa_K4103000 pfu
http://parts.igem.org/Part:BBa_K4103001 Conjugation origin Transfer Origin pIP501 319
http://parts.igem.org/Part:BBa_K4103003 Resistance marker SpecR 989
http://parts.igem.org/Part:BBa_K4103004 Origin of replication EcoORI 589
http://parts.igem.org/Part:BBa_K4103005 Origin of transfer OriTBsub 378
http://parts.igem.org/Part:BBa_K4103007 Resistance marker catA9 939
http://parts.igem.org/Part:BBa_K4103008 Insulator HDV-1 (3' Ribozyme Insulator) 85
http://parts.igem.org/Part:BBa_K4103009
http://parts.igem.org/Part:BBa_K4103016 OriTBsub
http://parts.igem.org/Part:BBa_K4103017 Terminator E. coli terminator 54
http://parts.igem.org/Part:BBa_K4103018 Promoter kanR promoter 101
http://parts.igem.org/Part:BBa_K4103019 Terminator kanR terminator 48
http://parts.igem.org/Part:BBa_K4103020 Coding kanR cds 816
http://parts.igem.org/Part:BBa_K4103021 Coding lacI cds 1083
http://parts.igem.org/Part:BBa_K4103022
http://parts.igem.org/Part:BBa_K4103023 Operator Lac operator 25
http://parts.igem.org/Part:BBa_K4103024 Insulator RiboJ54 Insulator 75
http://parts.igem.org/Part:BBa_K4103025
http://parts.igem.org/Part:BBa_K4103026 Coding Pfu (DNA polymerase) #4 2322
http://parts.igem.org/Part:BBa_K4103027 Coding Sso-7d #4 189
http://parts.igem.org/Part:BBa_K4103028 Linker TEV linker site #4 21
http://parts.igem.org/Part:BBa_K4103029 Coding fuGFP #4 732
http://parts.igem.org/Part:BBa_K4103030 Tag 10xHisTag #4 30
http://parts.igem.org/Part:BBa_K4103031 Tag R5 Silica binding purification tag #4 57
http://parts.igem.org/Part:BBa_K4103032 Coding Pfu (DNA polymerase) #2 2322
http://parts.igem.org/Part:BBa_K4103033 Coding Sso-7d #2 189
http://parts.igem.org/Part:BBa_K4103034 Linker TEV linker site 21
http://parts.igem.org/Part:BBa_K4103035 Coding fuGFP 732
http://parts.igem.org/Part:BBa_K4103036 Purification Tag 10xHisTag 30
http://parts.igem.org/Part:BBa_K4103037 Purification Tag R5 Silica binding purification tag 57
http://parts.igem.org/Part:BBa_K4103038 Coding ccdb 335
http://parts.igem.org/Part:BBa_K4103039 Homology Arm lacA 3' homology 408
http://parts.igem.org/Part:BBa_K4103040 Homology Arm amyE 5' homology 521
http://parts.igem.org/Part:BBa_K4103041 Homology Arm amyE 3' homology 498
http://parts.igem.org/Part:BBa_K4103042 Homology Arm thrC 5' homology 501
http://parts.igem.org/Part:BBa_K4103043 Homology Arm thrC 3' homology 429
http://parts.igem.org/Part:BBa_K4103044 Homology Arm lacA 5' homology arm 602
http://parts.igem.org/Part:BBa_K4103045 Secretion Tag AmyQ 93