Difference between revisions of "Team:UIUC Illinois"
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| − | <html lang="en"><head><meta charset="utf-8"/><meta content="width=device-width,initial-scale=1" name="viewport"/><title>apPETite | UIUCiGEM</title><link href="https://2021.igem.org/Template:UIUC_Illinois/css/indexCSS?action=raw&ctype=text/css" rel="stylesheet"/></head><body><nav class="navbar navbar-expand-xl fixed-top"><div class="container d-flex justify-content-between"><a class="navbar-brand" href="https://2021.igem.org/Team:UIUC_Illinois"><i class="navbar-logo"></i></a><button aria-controls="navbarNav" aria-expanded="false" aria-label="Toggle navigation" class="navbar-toggler" data-target="#navbarNav" data-toggle="collapse" type="button"><span class="navbar-toggler-icon"></span></button><div class="collapse navbar-collapse" id="navbarNav"><ul class="navbar-nav ml-auto"><li class="nav-item dropdown"><a aria-expanded="false" aria-haspopup="true" class="nav-link dropdown-toggle" data-toggle="dropdown" href="#" id="navbarTeamDropdown" role="button">Team</a><div aria-labelledby="navbarTeamDropdown" class="dropdown-menu"><a class="dropdown-item" href="https://2021.igem.org/Team:UIUC_Illinois/Team">Team</a><a class="dropdown-item" href="https://2021.igem.org/Team:UIUC_Illinois/Attributions">Attributions</a><a class="dropdown-item" href="https://2021.igem.org/Team:UIUC_Illinois/Collaborations">Collaborations</a></div></li><li class="nav-item dropdown"><a aria-expanded="false" aria-haspopup="true" class="nav-link dropdown-toggle" data-toggle="dropdown" href="#" id="navbarProjectDropdown" role="button">Project</a><div aria-labelledby="navbarProjectDropdown" class="dropdown-menu"><a class="dropdown-item" href="https://2021.igem.org/Team:UIUC_Illinois/Contribution">Contribution</a><a class="dropdown-item" href="https://2021.igem.org/Team:UIUC_Illinois/Description">Description</a><a class="dropdown-item" href="https://2021.igem.org/Team:UIUC_Illinois/Engineering">Engineering Success</a><a class="dropdown-item" href="https://2021.igem.org/Team:UIUC_Illinois/Package">Excellence in another area</a><a class="dropdown-item" href="https://2021.igem.org/Team:UIUC_Illinois/Experiments">Experiments</a><a class="dropdown-item" href="https://2021.igem.org/Team:UIUC_Illinois/Model">Model</a><a class="dropdown-item" href="https://2021.igem.org/Team:UIUC_Illinois/Notebook">Notebook</a><a class="dropdown-item" href="https://2021.igem.org/Team:UIUC_Illinois/Partnership">Partnership</a><a class="dropdown-item" href="https://2021.igem.org/Team:UIUC_Illinois/Implementation">Proposed Implementation</a><a class="dropdown-item" href="https://2021.igem.org/Team:UIUC_Illinois/Results">Results</a></div></li><li class="nav-item"><a class="nav-link" href="https://2021.igem.org/Team:UIUC_Illinois/Parts">Parts</a></li><li class="nav-item"><a class="nav-link" href="https://2021.igem.org/Team:UIUC_Illinois/Safety">Safety</a></li><li class="nav-item"><a class="nav-link" href="https://2021.igem.org/Team:UIUC_Illinois/Human_Practices">Human Practices</a></li></ul></div><div class="d-flex" id="themeSwitchWrapper"><i class="far fa-sun"></i><div id="themeSwitch"><label class="switch" for="themeSwitchInput"><input id="themeSwitchInput" type="checkbox"/><span class="slider round"></span></label></div><i class="far fa-moon"></i></div></div></nav><header class="d-flex justify-content-center align-items-center"><div class="container"><div class="card" style="width: 60rem; position: relative; left: 50%; transform: translateX(-50%);"><video autoplay="true" loop="" muted=""><source src="https://static.igem.org/mediawiki/2021/4/47/T--UIUC_Illinois--video--homepage.mp4" type="video/mp4"/></video></div></div></header><article><div class="container"><h1>Abstract</h1><h5>Polyethylene terephthalate, or PET, is a plastic abundantly used in packaging and containers. Though PET is the most recycled plastic in the U.S., 3.1 million tons are produced annually and its current recycling rate is only 31%. Traditional remediation methods such as pelleting yield less products for every PET regeneration. Also, the microbial remediation with wild-type PET degrading enzymes (commonly called PETase) cannot be used under many current recycling conditions and has low degradation rates. To generate enzymes with higher degradation rates and thermostability, we developed machine learning algorithms to generate a number of candidate enzyme sequences. These candidate enzymes were then expressed and purified from E. coli BL21 and tested for the improved degradation of PET plastic using the NanoDrop method for validation. Project apPETite is an attempt to provide a framework for developing an enzyme with enhanced PET plastic degradation.</h5><br/><h1>About Us</h1><h5>UIUC iGEM is engineering the polyethylene terephthalate (PET) degrading enzyme with improved thermostability and degradation rate</h5><p></p><br/><div class="row"><div class="col"><div class="card" style="width: 18rem;"><div class="card-img-top"><img src="https://static.igem.org/mediawiki/2021/c/cd/T--UIUC_Illinois--img--Home--jefrin.png"/></div><div class="card-body"><h5 class="card-title">Description</h5><p class="card-text">Learn more about our project here.</p><a class="btn btn-primary" href="https://2021.igem.org/Team:UIUC_Illinois/Description">Read more</a></div></div></div><div class="col"><div class="card" style="width: 18rem;"><div class="card-img-top"><img src="https://static.igem.org/mediawiki/2021/3/3e/T--UIUC_Illinois--img--Home--group.jpg"/></div><div class="card-body"><h5 class="card-title">Team</h5><p class="card-text">Meet our team!</p><a class="btn btn-primary" href="https://2021.igem.org/Team:UIUC_Illinois/Team">Read More</a></div></div></div><div class="col"><div class="card" style="width: 18rem;"><div class="card-img-top"><img src="https://static.igem.org/mediawiki/2021/1/14/T--UIUC_Illinois--img--Home--kristin.png"/></div><div class="card-body"><h5 class="card-title">Engineering Success</h5><p class="card-text">Learn more about our experimental design and process.</p><a class="btn btn-primary" href="https://2021.igem.org/Team:UIUC_Illinois/Engineering">Read more</a></div></div></div></div></div></article><footer><div class="container"><div class="row justify-content-center"><div id="footerTeamLogo"></div><div id="UIUCLogo"></div><div id="CABBILogo"></div><div id="IGBLogo"></div></div><p>iGEM UIUC_Illinois</p><p>Email: <a href="mailto:igembitsgoa@gmail.com">illinoisigem@gmail.com</a></p><p>Headquarter: <a href="https://www.igb.illinois.edu/">Carl R. Woese Institute for Genomic Biology</a></p><p>1206 W Gregory Dr. Urbana IL 61801</p><br/><p>Sample template built using the iGEM Wiki Starter Pack by BITS Goa.</p></div></footer><script src="https://2021.igem.org/Template:UIUC_Illinois/index-bundleJS?action=raw&ctype=text/javascript"></script></body></html> | + | <html lang="en"><head><meta charset="utf-8"/><meta content="width=device-width,initial-scale=1" name="viewport"/><title>apPETite | UIUCiGEM</title><link href="https://2021.igem.org/Template:UIUC_Illinois/css/indexCSS?action=raw&ctype=text/css" rel="stylesheet"/></head><body><nav class="navbar navbar-expand-xl fixed-top"><div class="container d-flex justify-content-between"><a class="navbar-brand" href="https://2021.igem.org/Team:UIUC_Illinois"><i class="navbar-logo"></i></a><button aria-controls="navbarNav" aria-expanded="false" aria-label="Toggle navigation" class="navbar-toggler" data-target="#navbarNav" data-toggle="collapse" type="button"><span class="navbar-toggler-icon"></span></button><div class="collapse navbar-collapse" id="navbarNav"><ul class="navbar-nav ml-auto"><li class="nav-item dropdown"><a aria-expanded="false" aria-haspopup="true" class="nav-link dropdown-toggle" data-toggle="dropdown" href="#" id="navbarTeamDropdown" role="button">Team</a><div aria-labelledby="navbarTeamDropdown" class="dropdown-menu"><a class="dropdown-item" href="https://2021.igem.org/Team:UIUC_Illinois/Team">Team</a><a class="dropdown-item" href="https://2021.igem.org/Team:UIUC_Illinois/Attributions">Attributions</a><a class="dropdown-item" href="https://2021.igem.org/Team:UIUC_Illinois/Collaborations">Collaborations</a></div></li><li class="nav-item dropdown"><a aria-expanded="false" aria-haspopup="true" class="nav-link dropdown-toggle" data-toggle="dropdown" href="#" id="navbarProjectDropdown" role="button">Project</a><div aria-labelledby="navbarProjectDropdown" class="dropdown-menu"><a class="dropdown-item" href="https://2021.igem.org/Team:UIUC_Illinois/Contribution">Contribution</a><a class="dropdown-item" href="https://2021.igem.org/Team:UIUC_Illinois/Description">Description</a><a class="dropdown-item" href="https://2021.igem.org/Team:UIUC_Illinois/Engineering">Engineering Success</a><a class="dropdown-item" href="https://2021.igem.org/Team:UIUC_Illinois/Package">Excellence in another area</a><a class="dropdown-item" href="https://2021.igem.org/Team:UIUC_Illinois/Experiments">Experiments</a><a class="dropdown-item" href="https://2021.igem.org/Team:UIUC_Illinois/Model">Model</a><a class="dropdown-item" href="https://2021.igem.org/Team:UIUC_Illinois/Notebook">Notebook</a><a class="dropdown-item" href="https://2021.igem.org/Team:UIUC_Illinois/Partnership">Partnership</a><a class="dropdown-item" href="https://2021.igem.org/Team:UIUC_Illinois/Implementation">Proposed Implementation</a><a class="dropdown-item" href="https://2021.igem.org/Team:UIUC_Illinois/Results">Results</a></div></li><li class="nav-item"><a class="nav-link" href="https://2021.igem.org/Team:UIUC_Illinois/Parts">Parts</a></li><li class="nav-item"><a class="nav-link" href="https://2021.igem.org/Team:UIUC_Illinois/Safety">Safety</a></li><li class="nav-item"><a class="nav-link" href="https://2021.igem.org/Team:UIUC_Illinois/Human_Practices">Human Practices</a></li></ul></div><div class="d-flex" id="themeSwitchWrapper"><i class="far fa-sun"></i><div id="themeSwitch"><label class="switch" for="themeSwitchInput"><input id="themeSwitchInput" type="checkbox"/><span class="slider round"></span></label></div><i class="far fa-moon"></i></div></div></nav><header class="d-flex justify-content-center align-items-center"><div class="container"><div class="card" style="width: 60rem; position: relative; left: 50%; transform: translateX(-50%);"><video autoplay="true" loop="" muted=""><source src="https://static.igem.org/mediawiki/2021/4/47/T--UIUC_Illinois--video--homepage.mp4" type="video/mp4"/></video></div></div></header><article><div class="container"><h1>Abstract</h1><h5>Polyethylene terephthalate, or PET, is a plastic abundantly used in packaging and containers. Though PET is the most recycled plastic in the U.S., 3.1 million tons are produced annually and its current recycling rate is only 31%. Traditional remediation methods such as pelleting yield less products for every PET regeneration. Also, the microbial remediation with wild-type PET degrading enzymes (commonly called PETase) cannot be used under many current recycling conditions and has low degradation rates. To generate enzymes with higher degradation rates and thermostability, we developed machine learning algorithms to generate a number of candidate enzyme sequences. These candidate enzymes were then expressed and purified from E. coli BL21 and tested for the improved degradation of PET plastic using the NanoDrop method for validation. Project apPETite is an attempt to provide a framework for developing an enzyme with enhanced PET plastic degradation.</h5><br/><h1>About Us</h1><h5>UIUC iGEM is engineering the polyethylene terephthalate (PET) degrading enzyme with improved thermostability and degradation rate</h5><p></p><br/><div class="row"><div class="col"><div class="card" style="width: 18rem;"><div class="card-img-top"><img src="https://static.igem.org/mediawiki/2021/c/cd/T--UIUC_Illinois--img--Home--jefrin.png"/></div><div class="card-body"><h5 class="card-title">Description</h5><p class="card-text">Learn more about our project here.</p><a class="btn btn-primary" href="https://2021.igem.org/Team:UIUC_Illinois/Description">Read more</a></div></div></div><div class="col"><div class="card" style="width: 18rem;"><div class="card-img-top"><img src="https://static.igem.org/mediawiki/2021/3/3e/T--UIUC_Illinois--img--Home--group.jpg"/></div><div class="card-body"><h5 class="card-title">Team</h5><p class="card-text">Meet our team!</p><a class="btn btn-primary" href="https://2021.igem.org/Team:UIUC_Illinois/Team">Read More</a></div></div></div><div class="col"><div class="card" style="width: 18rem;"><div class="card-img-top"><img src="https://static.igem.org/mediawiki/2021/1/14/T--UIUC_Illinois--img--Home--kristin.png"/></div><div class="card-body"><h5 class="card-title">Engineering Success</h5><p class="card-text">Learn more about our experimental design and process.</p><a class="btn btn-primary" href="https://2021.igem.org/Team:UIUC_Illinois/Engineering">Read more</a></div></div></div></div></div></article><footer><div class="container"><div class="row justify-content-center"><div id="footerTeamLogo"></div><div id="UIUCLogo"></div><div id="CABBILogo"></div><div id="IGBLogo"></div></div><p>iGEM UIUC_Illinois</p><p>Email: <a href="mailto:igembitsgoa@gmail.com">illinoisigem@gmail.com</a></p><p>Headquarter: <a href="https://www.igb.illinois.edu/">Carl R. Woese Institute for Genomic Biology</a></p><p>1206 W Gregory Dr. Urbana IL 61801</p><br/><p>Sample template built using the iGEM Wiki Starter Pack by BITS Goa.</p><p>2021 UIUC_Illinois[Judging Form](https://igem.org/2021_Judging_Form?id=3713)</p></div></footer><script src="https://2021.igem.org/Template:UIUC_Illinois/index-bundleJS?action=raw&ctype=text/javascript"></script></body></html> |
