The main goal of our project is to make a frugal biofoundry capable of producing high quantities of enzymes and other proteins of interest. To do that, our team designed a library of parts in order to test in a high-throughput way the combinations that would generate the best results. For this year, the most important part generated by our team was the pHT43 plasmid (BBa_K4103000), an improvement on (BBa_K1074001) from 2013 - this plasmid acts as a shuttle vector between E. coli and B. subtilis, and allows the efficient production and secretion of our protein-of-interest, with our team focusing efforts to use this vector for the production of Pfu-Sso7d Polymerase (BBa_K4103011) codon optimized for B. Subtilis using the software created by our team.
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{{Open_Science_Global/main}} | {{Open_Science_Global/main}} | ||
{{Open_Science_Global/navbar}} | {{Open_Science_Global/navbar}} | ||
| + | {{Open_Science_Global/secondary}} | ||
<html> | <html> | ||
<style> | <style> | ||
| − | + | @import url('https://fonts.googleapis.com/css2?family=Roboto&display=swap'); | |
| + | </style> | ||
| + | <!-------------------- HTML CONTENT --------------------------------> | ||
| − | + | <body> | |
| − | + | <div class="top-banner"></div> | |
| − | + | <div class="Subtitle"> | |
| − | + | <div class="Title">Part Collection</div> | |
| − | + | </div> | |
| − | + | <div class="container"> | |
| − | + | <div class="nav-menu"> | |
| − | + | <div class="submenu-title">Part Collection</div> | |
| − | + | <div class="submenu-content"> | |
| − | + | <a href="#Overview">Overview</a> | |
| − | + | <a href="#Basic Parts">Basic Parts</a> | |
| − | + | <a href="#Composite Parts">Composite Parts</a> | |
| − | + | </div> | |
| − | + | </div> | |
| − | + | <div class="text-container"> | |
| − | + | <div class=title-container><h2 id="Overview">Overview</h2></div> | |
| − | + | <div class="text"> | |
| − | + | <p> | |
| − | + | The main goal of our project is to make a frugal biofoundry capable of producing high quantities of enzymes and other proteins of interest. To do that, our team designed a library of parts in order to test in a high-throughput way the combinations that would generate the best results. For this year, the most important part generated by our team was the pHT43 plasmid (BBa_K4103000), an improvement on (BBa_K1074001) from 2013 - this plasmid acts as a shuttle vector between E. coli and B. subtilis, and allows the efficient production and secretion of our protein-of-interest, with our team focusing efforts to use this vector for the production of Pfu-Sso7d Polymerase (BBa_K4103011) codon optimized for B. Subtilis using the software created by our team. | |
| − | + | </p> | |
| − | + | </div> | |
| − | + | <div class="title-container"><h2 id="Basic Parts">Basic Parts</h2></div> | |
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<tr> | <tr> | ||
| − | + | <th class="tg-lp5f"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Part Name</span></th> | |
| − | + | <th class="tg-lp5f"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Type</span></th> | |
| − | + | <th class="tg-lp5f"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Description</span></th> | |
| − | + | <th class="tg-lp5f"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Length (bp)</span></th> | |
</tr> | </tr> | ||
| − | + | </thead> | |
| − | + | <tbody> | |
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103000" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103000</span></a></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">pfu</span></td> | |
| − | + | <td class="tg-c6of"></td> | |
| − | + | <td class="tg-c6of"></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103001" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103001</span></a></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Conjugation origin</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Transfer Origin pIP501</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">319</span></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103003" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103003</span></a></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Resistance marker</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">SpecR</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">989</span></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103004" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103004</span></a></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Origin of replication</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">EcoORI</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">589</span></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103005" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103005</span></a></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Origin of transfer</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">OriTBsub</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">378</span></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103007" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103007</span></a></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Resistance marker</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">catA9</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">939</span></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103008" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103008</span></a></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Insulator</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">HDV-1 (3' Ribozyme Insulator)</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">85</span></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103009" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103009</span></a></td> | |
| − | + | <td class="tg-c6of"></td> | |
| − | + | <td class="tg-c6of"></td> | |
| − | + | <td class="tg-c6of"></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103016" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103016</span></a></td> | |
| − | + | <td class="tg-c6of"></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">OriTBsub</span></td> | |
| − | + | <td class="tg-c6of"></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103017" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103017</span></a></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Terminator</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">E. coli terminator</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">54</span></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103018" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103018</span></a></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Promoter</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">kanR promoter</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">101</span></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103019" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103019</span></a></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Terminator</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">kanR terminator</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">48</span></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103020" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103020</span></a></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">kanR cds</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">816</span></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103021" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103021</span></a></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">lacI cds</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">1083</span></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103022" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103022</span></a></td> | |
| − | + | <td class="tg-c6of"></td> | |
| − | + | <td class="tg-c6of"></td> | |
| − | + | <td class="tg-c6of"></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103023" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103023</span></a></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Operator</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Lac operator</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">25</span></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103024" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103024</span></a></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Insulator</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">RiboJ54 Insulator</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">75</span></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103025" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103025</span></a></td> | |
| − | + | <td class="tg-c6of"></td> | |
| − | + | <td class="tg-c6of"></td> | |
| − | + | <td class="tg-c6of"></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103026" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103026</span></a></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Pfu (DNA polymerase) #4</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">2322</span></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103027" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103027</span></a></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Sso-7d #4</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">189</span></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103028" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103028</span></a></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Linker</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">TEV linker site #4</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">21</span></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103029" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103029</span></a></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">fuGFP #4</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">732</span></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103030" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103030</span></a></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Tag</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">10xHisTag #4</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">30</span></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103031" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103031</span></a></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Tag</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">R5 Silica binding purification tag #4</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">57</span></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103032" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103032</span></a></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Pfu (DNA polymerase) #2</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">2322</span></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103033" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103033</span></a></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Sso-7d #2</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">189</span></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103034" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103034</span></a></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Linker</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">TEV linker site</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">21</span></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103035" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103035</span></a></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">fuGFP</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">732</span></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103036" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103036</span></a></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Purification Tag</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">10xHisTag</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">30</span></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103037" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103037</span></a></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Purification Tag</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">R5 Silica binding purification tag</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">57</span></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103038" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103038</span></a></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">ccdb</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">335</span></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103039" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103039</span></a></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">lacA 3' homology</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">408</span></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103040" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103040</span></a></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">amyE 5' homology</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">521</span></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103041" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103041</span></a></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">amyE 3' homology</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">498</span></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103042" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103042</span></a></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">thrC 5' homology</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">501</span></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103043" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103043</span></a></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">thrC 3' homology</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">429</span></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103044" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103044</span></a></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">lacA 5' homology arm</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">602</span></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103045" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103045</span></a></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Secretion Tag</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">AmyQ</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">93</span></td> | |
</tr> | </tr> | ||
| − | + | </tbody> | |
| − | + | </table> | |
| − | + | <div class="title-container"> | |
| + | <h2 id="Composite Parts">Composite Parts</h2> | ||
| + | </div> | ||
| + | <style type="text/css"> | ||
| + | .tg { | ||
| + | border-collapse: collapse; | ||
| + | border-color: #ccc; | ||
| + | border-spacing: 0; | ||
| + | } | ||
| + | .tg td { | ||
| + | background-color: #fff; | ||
| + | border-color: #ccc; | ||
| + | border-style: solid; | ||
| + | border-width: 1px; | ||
| + | color: #333; | ||
| + | font-family: Roboto, Roboto; | ||
| + | font-size: 14px; | ||
| + | overflow: hidden; | ||
| + | padding: 10px 5px; | ||
| + | word-break: normal; | ||
| + | } | ||
| − | + | .tg th { | |
| − | + | background-color: #f0f0f0; | |
| − | + | border-color: #ccc; | |
| − | + | border-style: solid; | |
| − | + | border-width: 1px; | |
| − | + | color: #333; | |
| − | + | font-family: Roboto, Roboto; | |
| − | + | font-size: 14px; | |
| − | + | font-weight: normal; | |
| − | + | overflow: hidden; | |
| − | + | padding: 10px 5px; | |
| − | + | word-break: normal; | |
| − | + | } | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | .tg .tg-3rmm { | |
| − | + | background-color: #ffffff; | |
| − | + | border-color: inherit; | |
| + | color: #15C; | ||
| + | text-align: left; | ||
| + | text-decoration: underline; | ||
| + | vertical-align: top | ||
| + | } | ||
| + | |||
| + | .tg .tg-lp5f { | ||
| + | background-color: #efefef; | ||
| + | border-color: inherit; | ||
| + | font-family: "Roboto Black", Gadget, Roboto !important; | ||
| + | ; | ||
| + | text-align: center; | ||
| + | vertical-align: top | ||
| + | } | ||
| + | |||
| + | .tg .tg-3xi5 { | ||
| + | background-color: #ffffff; | ||
| + | border-color: inherit; | ||
| + | text-align: center; | ||
| + | vertical-align: top | ||
| + | } | ||
| + | |||
| + | .tg .tg-c6of { | ||
| + | background-color: #ffffff; | ||
| + | border-color: inherit; | ||
| + | text-align: left; | ||
| + | vertical-align: top | ||
| + | } | ||
| + | |||
| + | .tg { | ||
| + | align-self: center; | ||
| + | width: 80%; | ||
| + | } | ||
| + | </style> | ||
| + | <table class="tg"> | ||
| + | <thead> | ||
<tr> | <tr> | ||
| − | + | <th class="tg-lp5f"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Part Name</span></th> | |
| − | + | <th class="tg-lp5f"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Type</span></th> | |
| − | + | <th class="tg-lp5f"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Description</span></th> | |
| − | + | <th class="tg-lp5f"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Length (bp)</span></th> | |
</tr> | </tr> | ||
| − | + | </thead> | |
| − | + | <tbody> | |
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103000" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103000</span></a></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">pfu</span></td> | |
| − | + | <td class="tg-c6of"></td> | |
| − | + | <td class="tg-c6of"></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103001" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103001</span></a></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Conjugation origin</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Transfer Origin pIP501</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">319</span></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103003" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103003</span></a></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Resistance marker</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">SpecR</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">989</span></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103004" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103004</span></a></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Origin of replication</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">EcoORI</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">589</span></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103005" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103005</span></a></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Origin of transfer</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">OriTBsub</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">378</span></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103007" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103007</span></a></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Resistance marker</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">catA9</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">939</span></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103008" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103008</span></a></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Insulator</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">HDV-1 (3' Ribozyme Insulator)</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">85</span></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103009" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103009</span></a></td> | |
| − | + | <td class="tg-c6of"></td> | |
| − | + | <td class="tg-c6of"></td> | |
| − | + | <td class="tg-c6of"></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030016" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103016</span></a></td> | |
| − | + | <td class="tg-c6of"></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">OriTBsub</span></td> | |
| − | + | <td class="tg-c6of"></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030017" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103017</span></a></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Terminator</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">E. coli terminator</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">54</span></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030018" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103018</span></a></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Promoter</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">kanR promoter</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">101</span></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103019" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103019</span></a></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Terminator</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">kanR terminator</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">48</span></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030020" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103020</span></a></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">kanR cds</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">816</span></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030021" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103021</span></a></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">lacI cds</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">1083</span></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030022" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103022</span></a></td> | |
| − | + | <td class="tg-c6of"></td> | |
| − | + | <td class="tg-c6of"></td> | |
| − | + | <td class="tg-c6of"></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030023" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103023</span></a></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Operator</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Lac operator</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">25</span></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030024" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103024</span></a></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Insulator</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">RiboJ54 Insulator</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">75</span></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030025" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103025</span></a></td> | |
| − | + | <td class="tg-c6of"></td> | |
| − | + | <td class="tg-c6of"></td> | |
| − | + | <td class="tg-c6of"></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030026" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103026</span></a></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Pfu (DNA polymerase) #4</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">2322</span></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030027" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103027</span></a></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Sso-7d #4</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">189</span></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030028" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103028</span></a></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Linker</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">TEV linker site #4</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">21</span></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030029" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103029</span></a></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">fuGFP #4</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">732</span></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030030" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103030</span></a></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Tag</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">10xHisTag #4</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">30</span></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030031" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103031</span></a></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Tag</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">R5 Silica binding purification tag #4</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">57</span></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030032" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103032</span></a></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Pfu (DNA polymerase) #2</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">2322</span></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030033" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103033</span></a></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Sso-7d #2</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">189</span></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030034" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103034</span></a></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Linker</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">TEV linker site</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">21</span></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030035" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103035</span></a></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">fuGFP</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">732</span></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030036" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103036</span></a></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Purification Tag</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">10xHisTag</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">30</span></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030037" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103037</span></a></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Purification Tag</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">R5 Silica binding purification tag</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">57</span></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030038" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103038</span></a></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">ccdb</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">335</span></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030039" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103039</span></a></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">lacA 3' homology</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">408</span></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030040" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103040</span></a></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">amyE 5' homology</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">521</span></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030041" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103041</span></a></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">amyE 3' homology</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">498</span></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030042" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103042</span></a></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">thrC 5' homology</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">501</span></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030043" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103043</span></a></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">thrC 3' homology</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">429</span></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103044" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103044</span></a></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">lacA 5' homology arm</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">602</span></td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K410300" target="_blank"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103045</span></a></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Secretion Tag</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">AmyQ</span></td> | |
| − | + | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">93</span></td> | |
</tr> | </tr> | ||
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| − | + | </table> | |
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</body> | </body> | ||
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</html> | </html> | ||
Latest revision as of 01:24, 18 December 2021
Part Collection