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| The materials needed to build it are: | | The materials needed to build it are: |
| </p> | | </p> |
| + | <p><b>Materials</b></p> |
| </div> | | </div> |
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| <p> | | <p> |
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| </div> | | </div> |
| + | <div class="text"> |
| + | <h3>Frugal Bioreactor Version 2 based on David Ishee’s build</h3> |
| + | <p><b>Materials</b></p> |
| + | </div> |
| + | <p> |
| + | <style type="text/css"> |
| + | .tg {border-collapse:collapse;border-color:#ccc;border-spacing:0;} |
| + | .tg td{background-color:#fff;border-color:#ccc;border-style:solid;border-width:1px;color:#333; |
| + | font-family:Arial, sans-serif;font-size:14px;overflow:hidden;padding:10px 5px;word-break:normal;} |
| + | .tg th{background-color:#f0f0f0;border-color:#ccc;border-style:solid;border-width:1px;color:#333; |
| + | font-family:Arial, sans-serif;font-size:14px;font-weight:normal;overflow:hidden;padding:10px 5px;word-break:normal;} |
| + | .tg .tg-1wig{font-weight:bold;text-align:left;vertical-align:top} |
| + | .tg .tg-ktyi{background-color:#FFF;text-align:left;vertical-align:top} |
| + | .tg .tg-zb5k{color:#15C;text-align:left;text-decoration:underline;vertical-align:top} |
| + | .tg .tg-0lax{text-align:left;vertical-align:top} |
| + | .tg .tg-dm87{background-color:#FFF;color:#15C;text-align:left;text-decoration:underline;vertical-align:top} |
| + | </style> |
| + | <table class="tg"> |
| + | <thead> |
| + | <tr> |
| + | <th class="tg-1wig"><span style="font-weight:700;font-style:normal;text-decoration:none;color:#000;background-color:transparent">Item</span></th> |
| + | <th class="tg-1wig"><span style="font-weight:700;font-style:normal;text-decoration:none;color:#000;background-color:transparent">Cost</span></th> |
| + | </tr> |
| + | </thead> |
| + | <tbody> |
| + | <tr> |
| + | <td class="tg-ktyi"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000;background-color:transparent">The Weekend Brewer STherm7 Stopper Thermowell</span></td> |
| + | <td class="tg-zb5k"><a href="https://www.amazon.com/dp/B01KP4D0K2/?ref=idea_lv_dp_vv_d"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C;background-color:transparent">US$ 10.99</span></a></td> |
| + | </tr> |
| + | <tr> |
| + | <td class="tg-0lax"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000;background-color:transparent">FastRack 5 Gallon Glass Carboy</span></td> |
| + | <td class="tg-dm87"><a href="https://www.amazon.com/dp/B0007R8ZBC/?ref=idea_lv_dp_vv_d"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C;background-color:#FFF">US$ 42.57</span></a><span style="font-weight:400;font-style:normal;text-decoration:none;color:#0F1111;background-color:#FFF"> </span></td> |
| + | </tr> |
| + | <tr> |
| + | <td class="tg-0lax"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000;background-color:transparent">Heat Pad with Controller for Home Brewing</span></td> |
| + | <td class="tg-dm87"><a href="https://www.amazon.com/dp/B071RXYCMK/?ref=idea_lv_dp_vv_d"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C;background-color:#FFF">US$ 46.99</span></a></td> |
| + | </tr> |
| + | <tr> |
| + | <td class="tg-ktyi"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000;background-color:transparent">Brew Hauler - 5168 FE338 Nylon carboy carrier</span></td> |
| + | <td class="tg-dm87"><a href="https://www.amazon.com/dp/B071RXYCMK/?ref=idea_lv_dp_vv_d"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C;background-color:#FFF">US$ 11.99</span></a></td> |
| + | </tr> |
| + | <tr> |
| + | <td class="tg-0lax"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000;background-color:transparent">Tetra Whisper Air Pump 30 To 60 Gallons</span></td> |
| + | <td class="tg-dm87"><a href="https://www.amazon.com/dp/B004PB8SNQ/?ref=idea_lv_dp_vv_d"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C;background-color:#FFF">US$ 13.9</span></a><a href="https://www.amazon.com/dp/B004PB8SNQ/?ref=idea_lv_dp_vv_d"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C;background-color:transparent">8</span></a></td> |
| + | </tr> |
| + | <tr> |
| + | <td class="tg-ktyi"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000;background-color:transparent">Penn Plax Airline Tubing for Aquariums –Clear and Flexible Resists Kinking, 25 Feet Standard</span></td> |
| + | <td class="tg-dm87"><a href="https://www.amazon.com/dp/B0002563MW/?ref=idea_lv_dp_vv_d"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C;background-color:#FFF">US$ 3.</span></a><a href="https://www.amazon.com/dp/B0002563MW/?ref=idea_lv_dp_vv_d"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C;background-color:transparent">05</span></a></td> |
| + | </tr> |
| + | <tr> |
| + | <td class="tg-ktyi"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000;background-color:transparent">Pack of 10 Glass Tubing, 12 Inch Long, Borosilicate Glass, Mixed Size 4mm, 6mm,8mm, 10mm, 12mm</span></td> |
| + | <td class="tg-dm87"><a href="https://www.amazon.com/dp/B017ALRJJQ/?ref=idea_lv_dp_vv_d"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C;background-color:#FFF">US$ 20.99</span></a></td> |
| + | </tr> |
| + | <tr> |
| + | <td class="tg-ktyi"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000;background-color:transparent">Nite Ize GT32-2PK-33 Gear Tie, 32"</span></td> |
| + | <td class="tg-dm87"><a href="https://www.amazon.com/dp/B00SHBO186/ref=twister_B08YGN7Z69?_encoding=UTF8&th=1"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C;background-color:#FFF">US$ 11.37</span></a></td> |
| + | </tr> |
| + | </tbody> |
| + | </table> |
| + | </p> |
| + | <div class = "text"> |
| + | <p><b>Justification</b></p> |
| + | <p>At BioBlaze, we wanted to try David Ishee’s frugal bioreactor build for several reasons. First, it is huge and looks amazing. Second, the build is with inexpensive components that can be easily sourced via Amazon - only $162. Finally, there is no soldering or coding required. The most difficult thing about the build was using a flame to carefully bend a glass tube, but this was easily done using a Bunsen burner.</p> |
| + | <p><b>Instructions:</b></p> |
| + | <p><a href="https://odysee.com/@DavidIshee:d/bioreactor-build-for-personal-gene:c">David Ishee;s Frugal Bioreactor Build</a></p> |
| + | </div> |
| + | <div class = "text"> |
| + | <p><b>Functionality test:</b></p> |
| + | <p>A fluorescent E. coli strain resistant to ampicillin was successfully grown in 5 liters of LB + amp 100 µg/µl. </p> |
| + | <p><img src="https://static.igem.org/mediawiki/2021/c/cf/T--Open_Science_Global--figure5ecoli.jpeg " alt="Bioreactor with E. Coli"></p> |
| + | <p> |
| + | E. coli with fluorescence and ampicillin |
| + | resistance growing without UV light. |
| + | </p> |
| + | <p><img src="https://static.igem.org/mediawiki/2021/f/f7/T--Open_Science_Global--figure4glowing.jpeg" alt="Bioreactor with glowing E. coli"></p> |
| + | <p><b>E. coli with fluorescence and ampicillin resistance growing with UV light.</b></p> |
| | | |
| + | </div> |
| | | |
| + | <div class = "title-container"> |
| + | <h2><b>Friendzymes's Frugal Column Chromatography Unit</b></h2> |
| + | </div> |
| + | <p><embed src="https://static.igem.org/mediawiki/2021/4/49/T--Open_Science_Global--Friendzymess_Frugal_Column_Chromatography_Unit_.pdf |
| + | " width="100%" height="100%" /> </p> |
| + | <p><embed src="https://static.igem.org/mediawiki/2021/6/69/T--Open_Science_Global--Friendzymess_Frugal_Column_Chromatography_Unit_1.pdf" width="100%" height="100%" /> </p> |
| + | <p> <object data="https://static.igem.org/mediawiki/2021/0/07/T--Open_Science_Global--Friendzymess_Frugal_Column_Chromatography_Unit_2.pdf |
| + | "></object></p> |
| + | </div> |
| | | |
| </div> | | </div> |