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| @import url('https://fonts.googleapis.com/css2?family=Roboto&display=swap'); | | @import url('https://fonts.googleapis.com/css2?family=Roboto&display=swap'); |
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| The materials needed to build it are: | | The materials needed to build it are: |
| </p> | | </p> |
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| <p><b>Materials</b></p> | | <p><b>Materials</b></p> |
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| <b>a) Container and thermal insulation jacket</b> | | <b>a) Container and thermal insulation jacket</b> |
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| <p><b>Future Plans</b></p> | | <p><b>Future Plans</b></p> |
| <p>Designing an electronic circuit can lower costs when developing a product because electronic devices that fulfill a specific function are used. However, to ensure consistent and robust operation it is advisable to develop a Printed Circuit Board. It has developed the Printed Circuit Board design of our bioreactor using the EasyEDA open software. The next step will be to order the printing from a local supplier, solder the electronic components and perform the functional tests.</p> | | <p>Designing an electronic circuit can lower costs when developing a product because electronic devices that fulfill a specific function are used. However, to ensure consistent and robust operation it is advisable to develop a Printed Circuit Board. It has developed the Printed Circuit Board design of our bioreactor using the EasyEDA open software. The next step will be to order the printing from a local supplier, solder the electronic components and perform the functional tests.</p> |
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| <div class = "title-container"> | | <div class = "title-container"> |
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| </div> | | </div> |
| + | <div class="text"> |
| + | <h3>Frugal Bioreactor Version 2 based on David Ishee’s build</h3> |
| + | <p><b>Materials</b></p> |
| + | </div> |
| + | <p> |
| + | <style type="text/css"> |
| + | .tg {border-collapse:collapse;border-color:#ccc;border-spacing:0;} |
| + | .tg td{background-color:#fff;border-color:#ccc;border-style:solid;border-width:1px;color:#333; |
| + | font-family:Arial, sans-serif;font-size:14px;overflow:hidden;padding:10px 5px;word-break:normal;} |
| + | .tg th{background-color:#f0f0f0;border-color:#ccc;border-style:solid;border-width:1px;color:#333; |
| + | font-family:Arial, sans-serif;font-size:14px;font-weight:normal;overflow:hidden;padding:10px 5px;word-break:normal;} |
| + | .tg .tg-1wig{font-weight:bold;text-align:left;vertical-align:top} |
| + | .tg .tg-ktyi{background-color:#FFF;text-align:left;vertical-align:top} |
| + | .tg .tg-zb5k{color:#15C;text-align:left;text-decoration:underline;vertical-align:top} |
| + | .tg .tg-0lax{text-align:left;vertical-align:top} |
| + | .tg .tg-dm87{background-color:#FFF;color:#15C;text-align:left;text-decoration:underline;vertical-align:top} |
| + | </style> |
| + | <table class="tg"> |
| + | <thead> |
| + | <tr> |
| + | <th class="tg-1wig"><span style="font-weight:700;font-style:normal;text-decoration:none;color:#000;background-color:transparent">Item</span></th> |
| + | <th class="tg-1wig"><span style="font-weight:700;font-style:normal;text-decoration:none;color:#000;background-color:transparent">Cost</span></th> |
| + | </tr> |
| + | </thead> |
| + | <tbody> |
| + | <tr> |
| + | <td class="tg-ktyi"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000;background-color:transparent">The Weekend Brewer STherm7 Stopper Thermowell</span></td> |
| + | <td class="tg-zb5k"><a href="https://www.amazon.com/dp/B01KP4D0K2/?ref=idea_lv_dp_vv_d"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C;background-color:transparent">US$ 10.99</span></a></td> |
| + | </tr> |
| + | <tr> |
| + | <td class="tg-0lax"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000;background-color:transparent">FastRack 5 Gallon Glass Carboy</span></td> |
| + | <td class="tg-dm87"><a href="https://www.amazon.com/dp/B0007R8ZBC/?ref=idea_lv_dp_vv_d"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C;background-color:#FFF">US$ 42.57</span></a><span style="font-weight:400;font-style:normal;text-decoration:none;color:#0F1111;background-color:#FFF"> </span></td> |
| + | </tr> |
| + | <tr> |
| + | <td class="tg-0lax"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000;background-color:transparent">Heat Pad with Controller for Home Brewing</span></td> |
| + | <td class="tg-dm87"><a href="https://www.amazon.com/dp/B071RXYCMK/?ref=idea_lv_dp_vv_d"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C;background-color:#FFF">US$ 46.99</span></a></td> |
| + | </tr> |
| + | <tr> |
| + | <td class="tg-ktyi"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000;background-color:transparent">Brew Hauler - 5168 FE338 Nylon carboy carrier</span></td> |
| + | <td class="tg-dm87"><a href="https://www.amazon.com/dp/B071RXYCMK/?ref=idea_lv_dp_vv_d"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C;background-color:#FFF">US$ 11.99</span></a></td> |
| + | </tr> |
| + | <tr> |
| + | <td class="tg-0lax"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000;background-color:transparent">Tetra Whisper Air Pump 30 To 60 Gallons</span></td> |
| + | <td class="tg-dm87"><a href="https://www.amazon.com/dp/B004PB8SNQ/?ref=idea_lv_dp_vv_d"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C;background-color:#FFF">US$ 13.9</span></a><a href="https://www.amazon.com/dp/B004PB8SNQ/?ref=idea_lv_dp_vv_d"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C;background-color:transparent">8</span></a></td> |
| + | </tr> |
| + | <tr> |
| + | <td class="tg-ktyi"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000;background-color:transparent">Penn Plax Airline Tubing for Aquariums –Clear and Flexible Resists Kinking, 25 Feet Standard</span></td> |
| + | <td class="tg-dm87"><a href="https://www.amazon.com/dp/B0002563MW/?ref=idea_lv_dp_vv_d"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C;background-color:#FFF">US$ 3.</span></a><a href="https://www.amazon.com/dp/B0002563MW/?ref=idea_lv_dp_vv_d"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C;background-color:transparent">05</span></a></td> |
| + | </tr> |
| + | <tr> |
| + | <td class="tg-ktyi"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000;background-color:transparent">Pack of 10 Glass Tubing, 12 Inch Long, Borosilicate Glass, Mixed Size 4mm, 6mm,8mm, 10mm, 12mm</span></td> |
| + | <td class="tg-dm87"><a href="https://www.amazon.com/dp/B017ALRJJQ/?ref=idea_lv_dp_vv_d"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C;background-color:#FFF">US$ 20.99</span></a></td> |
| + | </tr> |
| + | <tr> |
| + | <td class="tg-ktyi"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000;background-color:transparent">Nite Ize GT32-2PK-33 Gear Tie, 32"</span></td> |
| + | <td class="tg-dm87"><a href="https://www.amazon.com/dp/B00SHBO186/ref=twister_B08YGN7Z69?_encoding=UTF8&th=1"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C;background-color:#FFF">US$ 11.37</span></a></td> |
| + | </tr> |
| + | </tbody> |
| + | </table> |
| + | </p> |
| + | <div class = "text"> |
| + | <p><b>Justification</b></p> |
| + | <p>At BioBlaze, we wanted to try David Ishee’s frugal bioreactor build for several reasons. First, it is huge and looks amazing. Second, the build is with inexpensive components that can be easily sourced via Amazon - only $162. Finally, there is no soldering or coding required. The most difficult thing about the build was using a flame to carefully bend a glass tube, but this was easily done using a Bunsen burner.</p> |
| + | <p><b>Instructions:</b></p> |
| + | <p><a href="https://odysee.com/@DavidIshee:d/bioreactor-build-for-personal-gene:c">David Ishee;s Frugal Bioreactor Build</a></p> |
| + | </div> |
| + | <div class = "text"> |
| + | <p><b>Functionality test:</b></p> |
| + | <p>A fluorescent E. coli strain resistant to ampicillin was successfully grown in 5 liters of LB + amp 100 µg/µl. </p> |
| + | <p><img src="https://static.igem.org/mediawiki/2021/c/cf/T--Open_Science_Global--figure5ecoli.jpeg " alt="Bioreactor with E. Coli"></p> |
| + | <p> |
| + | E. coli with fluorescence and ampicillin |
| + | resistance growing without UV light. |
| + | </p> |
| + | <p><img src="https://static.igem.org/mediawiki/2021/f/f7/T--Open_Science_Global--figure4glowing.jpeg" alt="Bioreactor with glowing E. coli"></p> |
| + | <p><b>E. coli with fluorescence and ampicillin resistance growing with UV light.</b></p> |
| | | |
| + | </div> |
| | | |
| + | <div class = "title-container"> |
| + | <h2><b>Friendzymes's Frugal Column Chromatography Unit</b></h2> |
| + | </div> |
| + | <p><embed src="https://static.igem.org/mediawiki/2021/4/49/T--Open_Science_Global--Friendzymess_Frugal_Column_Chromatography_Unit_.pdf |
| + | " width="100%" height="100%" /> </p> |
| + | <p><embed src="https://static.igem.org/mediawiki/2021/6/69/T--Open_Science_Global--Friendzymess_Frugal_Column_Chromatography_Unit_1.pdf" width="100%" height="100%" /> </p> |
| + | <p> <object data="https://static.igem.org/mediawiki/2021/0/07/T--Open_Science_Global--Friendzymess_Frugal_Column_Chromatography_Unit_2.pdf |
| + | "></object></p> |
| + | </div> |
| | | |
| </div> | | </div> |