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| {{Open_Science_Global/main}} | | {{Open_Science_Global/main}} |
| {{Open_Science_Global/navbar}} | | {{Open_Science_Global/navbar}} |
− | {{Open_Science_Global/mainStyles}} | + | {{Open_Science_Global/secondary}} |
| <html> | | <html> |
| <style> | | <style> |
| @import url('https://fonts.googleapis.com/css2?family=Roboto&display=swap'); | | @import url('https://fonts.googleapis.com/css2?family=Roboto&display=swap'); |
| + | img{ |
| + | width: 90%; |
| + | margin-left: 5%!important; |
| + | |
| + | } |
| + | table{ |
| + | width: 90%; |
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| </style> | | </style> |
| <!-------------------- HTML CONTENT --------------------------------> | | <!-------------------- HTML CONTENT --------------------------------> |
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| <div class="container"> | | <div class="container"> |
| | | |
− | | + | <div class="subsite-container"> |
| + | <div class = "submenu-title">Menu</div> |
| + | <div class = index-box> |
| + | <a href="https://2021.igem.org/Team:Open_Science_Global/Description">Description</a> |
| + | <a href="https://2021.igem.org/Team:Open_Science_Global/Engineering">Engineering Success</a> |
| + | <a href="https://2021.igem.org/Team:Open_Science_Global/Implementation">Proposed Implementation</a> |
| + | <a href="https://2021.igem.org/Team:Open_Science_Global/Proof_Of_Concept">Proof of Concept</a> |
| + | <a href="https://2021.igem.org/Team:Open_Science_Global/Software">Software</a> |
| + | <a href="https://2021.igem.org/Team:Open_Science_Global/Hardware">Hardware</a> |
| + | </div> |
| + | </div> |
| | | |
| <div class="nav-menu"> | | <div class="nav-menu"> |
− | <div class="submenu-title">Project</div> | + | <div class="submenu-title">Hardware</div> |
| | | |
| <div class="submenu-content"> | | <div class="submenu-content"> |
| <a href="#Introduction">Introduction</a> | | <a href="#Introduction">Introduction</a> |
− | <a href="#toolkit">A quick walk through on toolkit</a> | + | <a href="#bioreactors">Friendzymes's Frugal Bioreactors</a> |
| <a href="#cookbook">The Friendzymes Cookbook</a> | | <a href="#cookbook">The Friendzymes Cookbook</a> |
| <a href="#actions">Friendzymes Actions</a> | | <a href="#actions">Friendzymes Actions</a> |
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| <div class="text-container"> | | <div class="text-container"> |
| + | <div class = "title-container"> |
| <h2 id="Introduction">Introduction</h2> | | <h2 id="Introduction">Introduction</h2> |
| + | </div> |
| <div class="text"> | | <div class="text"> |
| <p> | | <p> |
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| are building to add to our biofoundries. | | are building to add to our biofoundries. |
| </p> | | </p> |
− | <li> | + | <li class = "custom-li"><b><mark>Frugal bioreactor</mark></b> |
− | <p><mark>Frugal bioreactor</mark></p>
| + | |
| <p>Necessary for producing the vessels of our enzymes, B. subtilis, in large amounts for them to | | <p>Necessary for producing the vessels of our enzymes, B. subtilis, in large amounts for them to |
| secrete our polymerases in large amounts. | | secrete our polymerases in large amounts. |
| </p> | | </p> |
| </li> | | </li> |
− | <li> | + | <li class = "custom-li"> <b><mark>Frugal Chromatography column</mark></b> |
− | <p><b><mark>Frugal Chromatography column</mark></b></p>
| + | |
| <p>Necessary for separating desired proteins of interest after our vessels have successfully | | <p>Necessary for separating desired proteins of interest after our vessels have successfully |
| expressed and secreted them. | | expressed and secreted them. |
| </p> | | </p> |
| </li> | | </li> |
− | <li> | + | <li class = "custom-li"><b><mark>Frugal Fluorescent Plate Reader</mark></b> |
− | <p><b><mark>Frugal Fluorescent Plate Reader</mark></b></p>
| + | |
| <p> Necessary for having a readout on the purity of the enzymes obtained through our chromatography | | <p> Necessary for having a readout on the purity of the enzymes obtained through our chromatography |
| column. | | column. |
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| </p> | | </p> |
| | | |
| + | |
| + | |
| + | </div> |
| + | |
| + | <div class = "title-container"> |
| + | <h2 id="bioreactors">Friendzymes's Frugal Bioreactors</h2> |
| + | </div> |
| + | <div class="text"> |
| + | <p> |
| + | Bioreactors play a key role in the production of proteins and enzymes for the development and global |
| + | deployment of biotechnology. These consist of vessels where a biological reaction or change takes |
| + | place. The biological systems involved can be enzymes, proteins, and microorganisms. By providing a |
| + | favorable growth environment, bioreactors meet the needs of the biological system involved so that a |
| + | high production yield is achieved. |
| + | </p> |
| + | <p>For the design of a bioreactor, it is necessary to control the operating parameters that favor cell |
| + | growth and metabolism of cells or enzymes. The main parameters are dissolved oxygen concentration, |
| + | pH, temperature, and mixing. Commercial solutions priced between $580 to approximately $4,500 for |
| + | 5-litre capacities are expensive for low-resource labs and do not allow for customization and |
| + | maintenance. The development of low-cost, open-source bioreactors could help develop a more |
| + | accessible biotechnological infrastructure that reduces financial barriers to the development of |
| + | future bioengineers. |
| + | |
| + | </p> |
| + | |
| + | <p>The Friendzymes Open Science Global team seeks to accelerate the deployment of hardware needed to |
| + | democratize biotechnology worldwide through the development of two bioreactor builds made with |
| + | accessible materials, open-source, automatic controls, and a low cost of USD 82 - 162 dollars. |
| + | </p> |
| + | <p><h3>References</h3></p> |
| + | <p>[1] Shang-Tian Yang, Chapter 1 - Bioprocessing from Biotechnology to Biorefinery, Editor(s): Shang-Tian Yang, Bioprocessing for Value-Added Products from Renewable Resources, Elsevier, 2007, Pages 1-24, ISBN 9780444521149, https://doi.org/10.1016/B978-044452114-9/50002-5.</p> |
| + | </div> |
| + | |
| + | <h3>Friendzymes Bioreactor Prototype 1.0</h3> |
| + | |
| + | <div class="text"> |
| + | <p><b>Design and Assembly</b></p> |
| + | <p>The design of the proposed bioreactor is of the batch type. It consists of three main parts: A) |
| + | Container and thermal insulation jacket , B) sensors and actuators and C) Electronic control system. |
| + | The materials needed to build it are: |
| + | </p> |
| + | <p><b>Materials</b></p> |
| + | </div> |
| + | |
| + | |
| + | <p> |
| + | <style type="text/css"> |
| + | .tg {border-collapse:collapse;border-color:#ccc;border-spacing:0;} |
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| + | </style> |
| + | <table class="tg"> |
| + | <thead> |
| + | <tr> |
| + | <th class="tg-1wig"><span style="font-weight:700;font-style:normal;text-decoration:none;color:#000;background-color:transparent">Item</span></th> |
| + | <th class="tg-amwm"><span style="font-weight:700;font-style:normal;text-decoration:none;color:#000;background-color:transparent">Cost</span></th> |
| + | </tr> |
| + | </thead> |
| + | <tbody> |
| + | <tr> |
| + | <td class="tg-0lax"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000;background-color:transparent">Esp32 2.4 GHz WiFi + Bluetooth</span></td> |
| + | <td class="tg-kry2"><a href="https://www.amazon.com/ESP-WROOM-32-desarrollo-procesador-microcontrolador-amplificadora/dp/B0718T232Z/ref=sr_1_1_sspa?__mk_es_US=%C3%85M%C3%85%C5%BD%C3%95%C3%91&dchild=1&keywords=esp32&qid=1629232801&sr=8-1-spons&psc=1&spLa=ZW5jcnlwdGVkUXVhbGlmaWVyPUFXRUpFMERHOUtGWEYmZW5jcnlwdGVkSWQ9QTA2MzcwNDMzQ01YTk1VR001UUlGJmVuY3J5cHRlZEFkSWQ9QTAyMDg0MzEyTzNRU1ZWVFJUQTFOJndpZGdldE5hbWU9c3BfYXRmJmFjdGlvbj1jbGlja1JlZGlyZWN0JmRvTm90TG9nQ2xpY2s9dHJ1ZQ=="><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C;background-color:transparent">USD 10.99</span></a></td> |
| + | </tr> |
| + | <tr> |
| + | <td class="tg-0lax"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000;background-color:transparent">Aquarium Fish Heater 50W</span></td> |
| + | <td class="tg-db9x"><a href="https://www.amazon.com/Calentador-acuario-temperatura-ajustable-termostato/dp/B07T3WPJH8/ref=sr_1_3_sspa?__mk_es_US=%C3%85M%C3%85%C5%BD%C3%95%C3%91&dchild=1&keywords=fish+heater&qid=1629232943&sr=8-3-spons&psc=1&spLa=ZW5jcnlwdGVkUXVhbGlmaWVyPUExQktRQ01WV1laMU9EJmVuY3J5cHRlZElkPUEwNDg4MzM4SktQODBHNktEUkdEJmVuY3J5cHRlZEFkSWQ9QTAzMzk0MDczOE5WNElWN1IxN0hLJndpZGdldE5hbWU9c3BfYXRmJmFjdGlvbj1jbGlja1JlZGlyZWN0JmRvTm90TG9nQ2xpY2s9dHJ1ZQ=="><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C;background-color:#FFF">USD 10.89</span></a><span style="font-weight:400;font-style:normal;text-decoration:none;color:#0F1111;background-color:#FFF"> </span></td> |
| + | </tr> |
| + | <tr> |
| + | <td class="tg-0lax"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000;background-color:transparent">Aquarium Airline Tubing</span></td> |
| + | <td class="tg-db9x"><a href="https://www.amazon.com/ALEGI-transparente-resistente-deformaci%C3%B3n-hidropon%C3%ADa/dp/B09433HZ3P/ref=sr_1_25_sspa?__mk_es_US=%C3%85M%C3%85%C5%BD%C3%95%C3%91&dchild=1&keywords=fish%2Btank%2Bhose&qid=1634792248&sr=8-25-spons&spLa=ZW5jcnlwdGVkUXVhbGlmaWVyPUExRUExVDI2WTdTS1NPJmVuY3J5cHRlZElkPUEwNDI0ODc5MTUzVkpOSDFCNDJDNyZlbmNyeXB0ZWRBZElkPUEwODM3NjM1MTdOVUZaOTJISkxPOSZ3aWRnZXROYW1lPXNwX210ZiZhY3Rpb249Y2xpY2tSZWRpcmVjdCZkb05vdExvZ0NsaWNrPXRydWU&th=1"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C;background-color:#FFF">US$ 4.99</span></a><span style="font-weight:400;font-style:normal;text-decoration:none;color:#0F1111;background-color:#FFF"> </span></td> |
| + | </tr> |
| + | <tr> |
| + | <td class="tg-0lax"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000;background-color:transparent">DS18B20 Temperature Sensor Module Kit</span></td> |
| + | <td class="tg-db9x"><a href="https://www.amazon.com/-/es/DS18B20-temperatura-inoxidable-impermeable-Raspberry/dp/B087JQ6MCP/ref=sr_1_4?__mk_es_US=%C3%85M%C3%85%C5%BD%C3%95%C3%91&dchild=1&keywords=DS18B20+sensor&qid=1630361756&sr=8-4"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C;background-color:#FFF">USD 7.99</span></a></td> |
| + | </tr> |
| + | <tr> |
| + | <td class="tg-ktyi"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000;background-color:transparent">10W Powerful Aquarium Air Pump</span></td> |
| + | <td class="tg-db9x"><a href="https://www.amazon.com/-/es/ajustable-silencioso-v%C3%A1lvulas-retenci%C3%B3n-silicona/dp/B0854CWH5M/ref=pb_allspark_dp_sims_pao_desktop_session_based_2/130-1163848-2732501?pd_rd_w=Yd4aC&pf_rd_p=e896123b-6614-49c5-873e-d532e726c2f0&pf_rd_r=FGKN3R0QMH9YZ8RHC5S8&pd_rd_r=492b88d4-3a7e-44b9-894c-12aaa5c86ea2&pd_rd_wg=NFSW3&pd_rd_i=B0854CWH5M&psc=1"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C;background-color:#FFF">USD 16.98</span></a></td> |
| + | </tr> |
| + | <tr> |
| + | <td class="tg-0lax"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000;background-color:transparent">OptoTriac MOC 3051</span></td> |
| + | <td class="tg-db9x"><a href="https://www.ebay.com/itm/284495568105?hash=item423d41dce9:g:QC4AAOSwMVFXIZPx"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C;background-color:#FFF">USD 1.07</span></a></td> |
| + | </tr> |
| + | <tr> |
| + | <td class="tg-ktyi"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000;background-color:transparent">BTA16 Triac</span></td> |
| + | <td class="tg-db9x"><a href="https://www.ebay.com/itm/303380345986?hash=item46a2e0b482:g:D4wAAOSw179ahGgV"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C;background-color:#FFF">USD 1.45</span></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C;background-color:#FFF"> </span></td> |
| + | </tr> |
| + | <tr> |
| + | <td class="tg-ktyi"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000;background-color:transparent">Resistor ( 100 Ohm, 200 Ohm)</span></td> |
| + | <td class="tg-baqh"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000;background-color:transparent">USD 1.00</span></td> |
| + | </tr> |
| + | <tr> |
| + | <td class="tg-ktyi"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000;background-color:transparent">Protoboard</span></td> |
| + | <td class="tg-db9x"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C;background-color:#FFF">USD 7.25</span></td> |
| + | </tr> |
| + | <tr> |
| + | <td class="tg-ktyi"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000;background-color:transparent">Insulation jacket</span></td> |
| + | <td class="tg-kry2"><a href="https://www.amazon.com/-/es/aislamiento-cer%C3%A1mica-CM-Ceramics-instrucciones-seguridad/dp/B015GD0QCW/ref=sr_1_10?__mk_es_US=%C3%85M%C3%85%C5%BD%C3%95%C3%91&dchild=1&keywords=glass%2Bwool&qid=1634738773&qsid=130-1163848-2732501&sr=8-10&sres=B07SKB6LQR%2CB01MT856N0%2CB08L748498%2CB001JYVDSE%2CB078JWWCBZ%2CB08ZC4GZ8B%2CB00GA9P5P0%2CB07HR93DSW%2CB015GD0QCW%2CB093BS9YV4%2CB0884CC1GG%2CB01N19O9VD%2CB076PJ23GP%2CB07QFS9SST%2CB089PT68W6%2CB096CJ3H2X%2CB07QD3XDDR%2CB01N8OJYSD%2CB086TT5566%2CB087R5HSNB&th=1"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C;background-color:transparent">USD 19.50</span></a></td> |
| + | </tr> |
| + | <tr> |
| + | <td class="tg-ktyi"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000;background-color:transparent">Total</span></td> |
| + | <td class="tg-baqh"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000;background-color:transparent">USD 82.11</span></td> |
| + | </tr> |
| + | </tbody> |
| + | </table> |
| + | </p> |
| + | <br> |
| + | <div class="text"> |
| + | <p> |
| + | <b>a) Container and thermal insulation jacket</b> |
| + | </p> |
| + | <p> |
| + | A 4-liter glass container was selected because it is a product that is easily accessible, very inexpensive, and is fully autoclavable. The lid can be made of metal or plastic, four holes will be made through which the heater, temperature sensor and two air hoses enter. The lid can be adapted to anyone of similar size. |
| + | </p> |
| + | <p> |
| + | To prevent the heat transfer and the temperature loss through the glass walls of the container, a ceramic fiber insulating blanket is used. This blanket is placed around the container to improve the temperature control efficiency of the biological system. |
| + | </p> |
| + | |
| + | <p><b>b) Sensors and Actuators</b></p> |
| + | <p>An aquarium air pump was used to oxygenate the culture in the bioreactor, which is an essential component of every bacterial culture. The air pump allows adequate aeration of the culture to obtain a good oxygen transfer rate (OTR) throughout the container. The lack of oxygen in the system can affect the performance of the growing process.</p> |
| + | <p>The DS18B20 is a waterproof Temperature Sensor Module Kit used to measure the temperature of the system at every point in time. This is very necessary because temperature is a key component which facilitates bacteria growth. This sensor has a digital output that connects to the esp32 microcontroller. The Esp32 2.4 GHz WiFi + Bluetooth component helps us receive feedback from the sensors for modifications to be made to the system. In addition, with the implementation of esp32 Internet of Things, historical data from the temperature sensor can be accessed, from 1 day to three months.</p> |
| + | |
| + | <p><b>c) Control and monitoring system</b></p> |
| + | <p>The device currently uses the Esp32 2.4 GHz WiFi + Bluetooth microcontrolador. With the PWM Pulse Width Modulation output control the voltage level is delivered to the heater and air pump. In this way it is possible to have a more precise control against external variations. Low-cost and opto device electronic components were considered to design a circuit that provides safety to the users. The two main electronic components used to achieve precise and safe control for the user were the MOC3051 optocoupler and the power device Triac BTA136. The optocoupler is a device that allows us to isolate the microcontroller control signals from the heater and air pump operating voltage through an internal light control. Thus, the external faults will not affect the control device and will keep it isolated and safe. The Triac BTA16 is a device designed for high performance, full wave alternating current general purpose applications where high noise immunity and high switching are required. Before carrying out the physical implementation of the circuit, we carried out a simulation in the LTspice software to verify the flow of currents and voltages. Then, we implement the circuit in the breadboard.</p> |
| + | <p><img src="https://static.igem.org/mediawiki/2021/6/65/T--Open_Science_Global--figure1hard.jpeg" alt="Diagram"></p> |
| + | <p>Figure 1. Schematic diagram and connections of the electronic circuit. This schematic diagram was made in the EasyEDA open software. It allows us to visualize the connection pins of each one of the devices and from there it is possible to create your own printed electronic PCB.</p> |
| + | <p><b>Future Plans</b></p> |
| + | <p>Designing an electronic circuit can lower costs when developing a product because electronic devices that fulfill a specific function are used. However, to ensure consistent and robust operation it is advisable to develop a Printed Circuit Board. It has developed the Printed Circuit Board design of our bioreactor using the EasyEDA open software. The next step will be to order the printing from a local supplier, solder the electronic components and perform the functional tests.</p> |
| + | </div> |
| + | |
| + | <div class = "title-container"> |
| + | <h2 id="bioreactors">Test</h2> |
| + | </div> |
| + | <div class="text"> |
| + | <p> |
| + | The bioreactor was home-made and all materials were sourced from local suppliers. Due to limited access to university laboratories during COVID 19, experimental tests on bacterial culture could not yet be performed. It is planned to carry out the experimental test with Bacillus Subtilis as soon as it is possible to access the laboratories in the city. Due to this, the following tests were carried out to verify the correct operation of the bioreactor: |
| + | </p> |
| + | <p> |
| + | <b>a) Temperature control and Remote monitoring system</b> |
| + | </p> |
| + | <p> |
| + | Temperature control is very important to guarantee optimal growth. The bioreactor was tested to regulate at 37 ° C, showing an 0.1% error. |
| + | </p> |
| + | <p> |
| + | <img src="https://static.igem.org/mediawiki/2021/2/2d/T--Open_Science_Global--figure2hard.png" alt="Temperature curve"> |
| + | </p> |
| + | <p> |
| + | Figure 2. Temperature control data logging at 37ºC. |
| + | </p> |
| + | <p> |
| + | <img src="https://static.igem.org/mediawiki/2021/4/4b/T--Open_Science_Global--figure3hard.jpeg" alt="Bioreactor"> |
| + | </p> |
| + | <p> |
| + | Figure 3. Remote monitoring test using Blynk software installed on a tablet. |
| + | </p> |
| + | |
| + | </div> |
| + | <div class="text"> |
| + | <h3>Frugal Bioreactor Version 2 based on David Ishee’s build</h3> |
| + | <p><b>Materials</b></p> |
| + | </div> |
| + | <p> |
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| + | <tr> |
| + | <th class="tg-1wig"><span style="font-weight:700;font-style:normal;text-decoration:none;color:#000;background-color:transparent">Item</span></th> |
| + | <th class="tg-1wig"><span style="font-weight:700;font-style:normal;text-decoration:none;color:#000;background-color:transparent">Cost</span></th> |
| + | </tr> |
| + | </thead> |
| + | <tbody> |
| + | <tr> |
| + | <td class="tg-ktyi"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000;background-color:transparent">The Weekend Brewer STherm7 Stopper Thermowell</span></td> |
| + | <td class="tg-zb5k"><a href="https://www.amazon.com/dp/B01KP4D0K2/?ref=idea_lv_dp_vv_d"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C;background-color:transparent">US$ 10.99</span></a></td> |
| + | </tr> |
| + | <tr> |
| + | <td class="tg-0lax"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000;background-color:transparent">FastRack 5 Gallon Glass Carboy</span></td> |
| + | <td class="tg-dm87"><a href="https://www.amazon.com/dp/B0007R8ZBC/?ref=idea_lv_dp_vv_d"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C;background-color:#FFF">US$ 42.57</span></a><span style="font-weight:400;font-style:normal;text-decoration:none;color:#0F1111;background-color:#FFF"> </span></td> |
| + | </tr> |
| + | <tr> |
| + | <td class="tg-0lax"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000;background-color:transparent">Heat Pad with Controller for Home Brewing</span></td> |
| + | <td class="tg-dm87"><a href="https://www.amazon.com/dp/B071RXYCMK/?ref=idea_lv_dp_vv_d"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C;background-color:#FFF">US$ 46.99</span></a></td> |
| + | </tr> |
| + | <tr> |
| + | <td class="tg-ktyi"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000;background-color:transparent">Brew Hauler - 5168 FE338 Nylon carboy carrier</span></td> |
| + | <td class="tg-dm87"><a href="https://www.amazon.com/dp/B071RXYCMK/?ref=idea_lv_dp_vv_d"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C;background-color:#FFF">US$ 11.99</span></a></td> |
| + | </tr> |
| + | <tr> |
| + | <td class="tg-0lax"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000;background-color:transparent">Tetra Whisper Air Pump 30 To 60 Gallons</span></td> |
| + | <td class="tg-dm87"><a href="https://www.amazon.com/dp/B004PB8SNQ/?ref=idea_lv_dp_vv_d"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C;background-color:#FFF">US$ 13.9</span></a><a href="https://www.amazon.com/dp/B004PB8SNQ/?ref=idea_lv_dp_vv_d"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C;background-color:transparent">8</span></a></td> |
| + | </tr> |
| + | <tr> |
| + | <td class="tg-ktyi"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000;background-color:transparent">Penn Plax Airline Tubing for Aquariums –Clear and Flexible Resists Kinking, 25 Feet Standard</span></td> |
| + | <td class="tg-dm87"><a href="https://www.amazon.com/dp/B0002563MW/?ref=idea_lv_dp_vv_d"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C;background-color:#FFF">US$ 3.</span></a><a href="https://www.amazon.com/dp/B0002563MW/?ref=idea_lv_dp_vv_d"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C;background-color:transparent">05</span></a></td> |
| + | </tr> |
| + | <tr> |
| + | <td class="tg-ktyi"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000;background-color:transparent">Pack of 10 Glass Tubing, 12 Inch Long, Borosilicate Glass, Mixed Size 4mm, 6mm,8mm, 10mm, 12mm</span></td> |
| + | <td class="tg-dm87"><a href="https://www.amazon.com/dp/B017ALRJJQ/?ref=idea_lv_dp_vv_d"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C;background-color:#FFF">US$ 20.99</span></a></td> |
| + | </tr> |
| + | <tr> |
| + | <td class="tg-ktyi"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000;background-color:transparent">Nite Ize GT32-2PK-33 Gear Tie, 32"</span></td> |
| + | <td class="tg-dm87"><a href="https://www.amazon.com/dp/B00SHBO186/ref=twister_B08YGN7Z69?_encoding=UTF8&th=1"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C;background-color:#FFF">US$ 11.37</span></a></td> |
| + | </tr> |
| + | </tbody> |
| + | </table> |
| + | </p> |
| + | <div class = "text"> |
| + | <p><b>Justification</b></p> |
| + | <p>At BioBlaze, we wanted to try David Ishee’s frugal bioreactor build for several reasons. First, it is huge and looks amazing. Second, the build is with inexpensive components that can be easily sourced via Amazon - only $162. Finally, there is no soldering or coding required. The most difficult thing about the build was using a flame to carefully bend a glass tube, but this was easily done using a Bunsen burner.</p> |
| + | <p><b>Instructions:</b></p> |
| + | <p><a href="https://odysee.com/@DavidIshee:d/bioreactor-build-for-personal-gene:c">David Ishee;s Frugal Bioreactor Build</a></p> |
| + | </div> |
| + | <div class = "text"> |
| + | <p><b>Functionality test:</b></p> |
| + | <p>A fluorescent E. coli strain resistant to ampicillin was successfully grown in 5 liters of LB + amp 100 µg/µl. </p> |
| + | <p><img src="https://static.igem.org/mediawiki/2021/c/cf/T--Open_Science_Global--figure5ecoli.jpeg " alt="Bioreactor with E. Coli"></p> |
| + | <p> |
| + | E. coli with fluorescence and ampicillin |
| + | resistance growing without UV light. |
| + | </p> |
| + | <p><img src="https://static.igem.org/mediawiki/2021/f/f7/T--Open_Science_Global--figure4glowing.jpeg" alt="Bioreactor with glowing E. coli"></p> |
| + | <p><b>E. coli with fluorescence and ampicillin resistance growing with UV light.</b></p> |
| + | |
| + | </div> |
| + | |
| + | <div class = "title-container"> |
| + | <h2><b>Friendzymes's Frugal Column Chromatography Unit</b></h2> |
| + | </div> |
| + | <p><embed src="https://static.igem.org/mediawiki/2021/4/49/T--Open_Science_Global--Friendzymess_Frugal_Column_Chromatography_Unit_.pdf |
| + | " width="100%" height="100%" /> </p> |
| + | <p><embed src="https://static.igem.org/mediawiki/2021/6/69/T--Open_Science_Global--Friendzymess_Frugal_Column_Chromatography_Unit_1.pdf" width="100%" height="100%" /> </p> |
| + | <p> <object data="https://static.igem.org/mediawiki/2021/0/07/T--Open_Science_Global--Friendzymess_Frugal_Column_Chromatography_Unit_2.pdf |
| + | "></object></p> |
| </div> | | </div> |
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| </div> | | </div> |
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