The main goal of our project is to make a frugal biofoundry capable of producing high quantities of enzymes and other proteins of interest. To do that, our team designed a library of parts in order to test in a high-throughput way the combinations that would generate the best results. For this year, the most important part generated by our team was the pHT43 plasmid (BBa_K4103000), an improvement on (BBa_K1074001) from 2013 - this plasmid acts as a shuttle vector between E. coli and B. subtilis, and allows the efficient production and secretion of our protein-of-interest, with our team focusing efforts to use this vector for the production of Pfu-Sso7d Polymerase (BBa_K4103011) codon optimized for B. Subtilis using the software created by our team.
Difference between revisions of "Team:Open Science Global/Part Collection"
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{{Open_Science_Global/navbar}} | {{Open_Science_Global/navbar}} | ||
| + | {{Open_Science_Global/secondary}} | ||
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<!-------------------- HTML CONTENT --------------------------------> | <!-------------------- HTML CONTENT --------------------------------> | ||
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<div class="container"> | <div class="container"> | ||
| − | + | <div class="subsite-container"> | |
| + | <div class = "submenu-title">Results</div> | ||
| + | <div class = index-box> | ||
| + | <a href="https://2021.igem.org/Team:Open_Science_Global/Wetware">Wetware</a> | ||
| + | <a href="https://2021.igem.org/Team:Open_Science_Global/Parts">Parts</a> | ||
| + | <a href="https://2021.igem.org/Team:Open_Science_Global/Part_Collection">Parts Collection</a> | ||
| + | <a href="https://2021.igem.org/Team:Open_Science_Global/Notebook">Notebook</a> | ||
| + | </div> | ||
| + | </div> | ||
<div class="nav-menu"> | <div class="nav-menu"> | ||
| Line 384: | Line 41: | ||
<div class="text-container"> | <div class="text-container"> | ||
| + | <div class = title-container> | ||
<h2 id="Overview">Overview</h2> | <h2 id="Overview">Overview</h2> | ||
| + | </div> | ||
<div class="text"> | <div class="text"> | ||
<p> | <p> | ||
| Line 390: | Line 49: | ||
</p> | </p> | ||
</div> | </div> | ||
| − | + | <div class = "title-container"> | |
<h2 id="Basic Parts">Basic Parts</h2> | <h2 id="Basic Parts">Basic Parts</h2> | ||
| − | + | </div> | |
<style type="text/css"> | <style type="text/css"> | ||
.tg {border-collapse:collapse;border-color:#ccc;border-spacing:0;} | .tg {border-collapse:collapse;border-color:#ccc;border-spacing:0;} | ||
| Line 432: | Line 91: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103003</span></td> | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103003" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103003</span></td> |
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Resistance marker</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Resistance marker</span></td> | ||
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">SpecR</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">SpecR</span></td> | ||
| Line 438: | Line 97: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103004</span></td> | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103004" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103004</span></td> |
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Origin of replication</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Origin of replication</span></td> | ||
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">EcoORI</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">EcoORI</span></td> | ||
| Line 456: | Line 115: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103008</span></td> | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103008" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103008</span></td> |
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Insulator</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Insulator</span></td> | ||
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">HDV-1 (3' Ribozyme Insulator)</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">HDV-1 (3' Ribozyme Insulator)</span></td> | ||
| Line 462: | Line 121: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103009</span></td> | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103009" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103009</span></td> |
<td class="tg-c6of"></td> | <td class="tg-c6of"></td> | ||
<td class="tg-c6of"></td> | <td class="tg-c6of"></td> | ||
| Line 468: | Line 127: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103016</span></td> | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103016" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103016</span></td> |
<td class="tg-c6of"></td> | <td class="tg-c6of"></td> | ||
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">OriTBsub</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">OriTBsub</span></td> | ||
| Line 474: | Line 133: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103017</span></td> | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103017" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103017</span></td> |
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Terminator</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Terminator</span></td> | ||
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">E. coli terminator</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">E. coli terminator</span></td> | ||
| Line 480: | Line 139: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103018</span></td> | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103018" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103018</span></td> |
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Promoter</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Promoter</span></td> | ||
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">kanR promoter</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">kanR promoter</span></td> | ||
| Line 492: | Line 151: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103020</span></td> | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103020" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103020</span></td> |
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td> | ||
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">kanR cds</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">kanR cds</span></td> | ||
| Line 498: | Line 157: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103021</span></td> | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103021" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103021</span></td> |
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td> | ||
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">lacI cds</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">lacI cds</span></td> | ||
| Line 504: | Line 163: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103022</span></td> | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103022" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103022</span></td> |
<td class="tg-c6of"></td> | <td class="tg-c6of"></td> | ||
<td class="tg-c6of"></td> | <td class="tg-c6of"></td> | ||
| Line 510: | Line 169: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103023</span></td> | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103023" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103023</span></td> |
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Operator</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Operator</span></td> | ||
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Lac operator</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Lac operator</span></td> | ||
| Line 516: | Line 175: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103024</span></td> | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103024" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103024</span></td> |
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Insulator</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Insulator</span></td> | ||
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">RiboJ54 Insulator</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">RiboJ54 Insulator</span></td> | ||
| Line 522: | Line 181: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103025</span></td> | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103025" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103025</span></td> |
<td class="tg-c6of"></td> | <td class="tg-c6of"></td> | ||
<td class="tg-c6of"></td> | <td class="tg-c6of"></td> | ||
| Line 528: | Line 187: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103026</span></td> | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103026" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103026</span></td> |
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td> | ||
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Pfu (DNA polymerase) #4</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Pfu (DNA polymerase) #4</span></td> | ||
| Line 534: | Line 193: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103027</span></td> | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103027" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103027</span></td> |
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td> | ||
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Sso-7d #4</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Sso-7d #4</span></td> | ||
| Line 540: | Line 199: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103028</span></td> | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103028" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103028</span></td> |
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Linker</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Linker</span></td> | ||
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">TEV linker site #4</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">TEV linker site #4</span></td> | ||
| Line 546: | Line 205: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103029</span></td> | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103029" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103029</span></td> |
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td> | ||
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">fuGFP #4</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">fuGFP #4</span></td> | ||
| Line 552: | Line 211: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103030</span></td> | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103030" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103030</span></td> |
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Tag</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Tag</span></td> | ||
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">10xHisTag #4</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">10xHisTag #4</span></td> | ||
| Line 558: | Line 217: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103031</span></td> | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103031" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103031</span></td> |
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Tag</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Tag</span></td> | ||
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">R5 Silica binding purification tag #4</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">R5 Silica binding purification tag #4</span></td> | ||
| Line 564: | Line 223: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103032</span></td> | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103032" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103032</span></td> |
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td> | ||
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Pfu (DNA polymerase) #2</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Pfu (DNA polymerase) #2</span></td> | ||
| Line 570: | Line 229: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103033</span></td> | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103033" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103033</span></td> |
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td> | ||
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Sso-7d #2</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Sso-7d #2</span></td> | ||
| Line 576: | Line 235: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103034</span></td> | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103034" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103034</span></td> |
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Linker</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Linker</span></td> | ||
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">TEV linker site</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">TEV linker site</span></td> | ||
| Line 582: | Line 241: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103035</span></td> | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103035" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103035</span></td> |
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td> | ||
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">fuGFP</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">fuGFP</span></td> | ||
| Line 588: | Line 247: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103036</span></td> | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103036" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103036</span></td> |
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Purification Tag</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Purification Tag</span></td> | ||
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">10xHisTag</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">10xHisTag</span></td> | ||
| Line 594: | Line 253: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103037</span></td> | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103037" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103037</span></td> |
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Purification Tag</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Purification Tag</span></td> | ||
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">R5 Silica binding purification tag</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">R5 Silica binding purification tag</span></td> | ||
| Line 600: | Line 259: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103038</span></td> | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103038" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103038</span></td> |
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td> | ||
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">ccdb</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">ccdb</span></td> | ||
| Line 606: | Line 265: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103039</span></td> | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103039" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103039</span></td> |
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td> | ||
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">lacA 3' homology</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">lacA 3' homology</span></td> | ||
| Line 612: | Line 271: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103040</span></td> | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103040" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103040</span></td> |
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td> | ||
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">amyE 5' homology</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">amyE 5' homology</span></td> | ||
| Line 618: | Line 277: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103041</span></td> | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103041" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103041</span></td> |
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td> | ||
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">amyE 3' homology</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">amyE 3' homology</span></td> | ||
| Line 624: | Line 283: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103042</span></td> | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103042" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103042</span></td> |
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td> | ||
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">thrC 5' homology</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">thrC 5' homology</span></td> | ||
| Line 630: | Line 289: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103043</span></td> | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103043" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103043</span></td> |
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td> | ||
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">thrC 3' homology</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">thrC 3' homology</span></td> | ||
| Line 642: | Line 301: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103045</span></td> | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103045" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103045</span></td> |
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Secretion Tag</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Secretion Tag</span></td> | ||
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">AmyQ</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">AmyQ</span></td> | ||
| Line 651: | Line 310: | ||
| − | + | <div class = "title-container"> | |
<h2 id="Composite Parts">Composite Parts</h2> | <h2 id="Composite Parts">Composite Parts</h2> | ||
| + | </div> | ||
<style type="text/css"> | <style type="text/css"> | ||
.tg {border-collapse:collapse;border-color:#ccc;border-spacing:0;} | .tg {border-collapse:collapse;border-color:#ccc;border-spacing:0;} | ||
| Line 693: | Line 353: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103003</span></td> | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103003" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103003</span></td> |
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Resistance marker</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Resistance marker</span></td> | ||
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">SpecR</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">SpecR</span></td> | ||
| Line 699: | Line 359: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103004</span></td> | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103004" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103004</span></td> |
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Origin of replication</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Origin of replication</span></td> | ||
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">EcoORI</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">EcoORI</span></td> | ||
| Line 717: | Line 377: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103008</span></td> | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103008" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103008</span></td> |
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Insulator</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Insulator</span></td> | ||
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">HDV-1 (3' Ribozyme Insulator)</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">HDV-1 (3' Ribozyme Insulator)</span></td> | ||
| Line 723: | Line 383: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103009</span></td> | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K4103009" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103009</span></td> |
<td class="tg-c6of"></td> | <td class="tg-c6of"></td> | ||
<td class="tg-c6of"></td> | <td class="tg-c6of"></td> | ||
| Line 729: | Line 389: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103016</span></td> | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030016" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103016</span></td> |
<td class="tg-c6of"></td> | <td class="tg-c6of"></td> | ||
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">OriTBsub</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">OriTBsub</span></td> | ||
| Line 735: | Line 395: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103017</span></td> | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030017" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103017</span></td> |
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Terminator</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Terminator</span></td> | ||
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">E. coli terminator</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">E. coli terminator</span></td> | ||
| Line 741: | Line 401: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103018</span></td> | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030018" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103018</span></td> |
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Promoter</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Promoter</span></td> | ||
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">kanR promoter</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">kanR promoter</span></td> | ||
| Line 753: | Line 413: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103020</span></td> | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030020" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103020</span></td> |
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td> | ||
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">kanR cds</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">kanR cds</span></td> | ||
| Line 759: | Line 419: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103021</span></td> | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030021" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103021</span></td> |
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td> | ||
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">lacI cds</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">lacI cds</span></td> | ||
| Line 765: | Line 425: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103022</span></td> | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030022" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103022</span></td> |
<td class="tg-c6of"></td> | <td class="tg-c6of"></td> | ||
<td class="tg-c6of"></td> | <td class="tg-c6of"></td> | ||
| Line 771: | Line 431: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103023</span></td> | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030023" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103023</span></td> |
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Operator</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Operator</span></td> | ||
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Lac operator</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Lac operator</span></td> | ||
| Line 777: | Line 437: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103024</span></td> | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030024" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103024</span></td> |
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Insulator</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Insulator</span></td> | ||
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">RiboJ54 Insulator</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">RiboJ54 Insulator</span></td> | ||
| Line 783: | Line 443: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103025</span></td> | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030025" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103025</span></td> |
<td class="tg-c6of"></td> | <td class="tg-c6of"></td> | ||
<td class="tg-c6of"></td> | <td class="tg-c6of"></td> | ||
| Line 789: | Line 449: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103026</span></td> | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030026" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103026</span></td> |
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td> | ||
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Pfu (DNA polymerase) #4</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Pfu (DNA polymerase) #4</span></td> | ||
| Line 795: | Line 455: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103027</span></td> | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030027" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103027</span></td> |
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td> | ||
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Sso-7d #4</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Sso-7d #4</span></td> | ||
| Line 801: | Line 461: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103028</span></td> | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030028" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103028</span></td> |
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Linker</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Linker</span></td> | ||
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">TEV linker site #4</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">TEV linker site #4</span></td> | ||
| Line 807: | Line 467: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103029</span></td> | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030029" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103029</span></td> |
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td> | ||
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">fuGFP #4</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">fuGFP #4</span></td> | ||
| Line 813: | Line 473: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103030</span></td> | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030030" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103030</span></td> |
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Tag</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Tag</span></td> | ||
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">10xHisTag #4</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">10xHisTag #4</span></td> | ||
| Line 819: | Line 479: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103031</span></td> | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030031" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103031</span></td> |
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Tag</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Tag</span></td> | ||
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">R5 Silica binding purification tag #4</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">R5 Silica binding purification tag #4</span></td> | ||
| Line 825: | Line 485: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103032</span></td> | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030032" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103032</span></td> |
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td> | ||
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Pfu (DNA polymerase) #2</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Pfu (DNA polymerase) #2</span></td> | ||
| Line 831: | Line 491: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103033</span></td> | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030033" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103033</span></td> |
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td> | ||
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Sso-7d #2</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Sso-7d #2</span></td> | ||
| Line 837: | Line 497: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103034</span></td> | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030034" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103034</span></td> |
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Linker</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Linker</span></td> | ||
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">TEV linker site</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">TEV linker site</span></td> | ||
| Line 843: | Line 503: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103035</span></td> | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030035" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103035</span></td> |
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td> | ||
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">fuGFP</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">fuGFP</span></td> | ||
| Line 849: | Line 509: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103036</span></td> | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030036" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103036</span></td> |
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Purification Tag</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Purification Tag</span></td> | ||
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">10xHisTag</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">10xHisTag</span></td> | ||
| Line 855: | Line 515: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103037</span></td> | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030037" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103037</span></td> |
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Purification Tag</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Purification Tag</span></td> | ||
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">R5 Silica binding purification tag</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">R5 Silica binding purification tag</span></td> | ||
| Line 861: | Line 521: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103038</span></td> | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030038" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103038</span></td> |
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Coding</span></td> | ||
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">ccdb</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">ccdb</span></td> | ||
| Line 867: | Line 527: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103039</span></td> | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030039" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103039</span></td> |
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td> | ||
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">lacA 3' homology</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">lacA 3' homology</span></td> | ||
| Line 873: | Line 533: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103040</span></td> | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030040" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103040</span></td> |
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td> | ||
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">amyE 5' homology</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">amyE 5' homology</span></td> | ||
| Line 879: | Line 539: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103041</span></td> | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030041" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103041</span></td> |
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td> | ||
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">amyE 3' homology</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">amyE 3' homology</span></td> | ||
| Line 885: | Line 545: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103042</span></td> | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030042" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103042</span></td> |
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td> | ||
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">thrC 5' homology</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">thrC 5' homology</span></td> | ||
| Line 891: | Line 551: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103043</span></td> | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K41030043" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103043</span></td> |
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Homology Arm</span></td> | ||
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">thrC 3' homology</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">thrC 3' homology</span></td> | ||
| Line 903: | Line 563: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="tg-3rmm"><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103045</span></td> | + | <td class="tg-3rmm"><a href="http://parts.igem.org/Part:BBa_K410300" target="_blank" rel="noopener noreferrer"></a><span style="font-weight:400;font-style:normal;text-decoration:underline;color:#15C">http://parts.igem.org/Part:BBa_K4103045</span></td> |
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Secretion Tag</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">Secretion Tag</span></td> | ||
<td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">AmyQ</span></td> | <td class="tg-3xi5"><span style="font-weight:400;font-style:normal;text-decoration:none;color:#000">AmyQ</span></td> | ||
| Line 911: | Line 571: | ||
</table> | </table> | ||
| − | </div> | + | |
| + | |||
| + | |||
| + | </div> | ||
| + | |||
| + | |||
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<footer> | <footer> | ||
Revision as of 00:09, 22 October 2021
Part Collection
Overview
Basic Parts
| Part Name | Type | Description | Length (bp) |
|---|---|---|---|
| http://parts.igem.org/Part:BBa_K4103000 | pfu | ||
| http://parts.igem.org/Part:BBa_K4103001 | Conjugation origin | Transfer Origin pIP501 | 319 |
| http://parts.igem.org/Part:BBa_K4103003 | Resistance marker | SpecR | 989 |
| http://parts.igem.org/Part:BBa_K4103004 | Origin of replication | EcoORI | 589 |
| http://parts.igem.org/Part:BBa_K4103005 | Origin of transfer | OriTBsub | 378 |
| http://parts.igem.org/Part:BBa_K4103007 | Resistance marker | catA9 | 939 |
| http://parts.igem.org/Part:BBa_K4103008 | Insulator | HDV-1 (3' Ribozyme Insulator) | 85 |
| http://parts.igem.org/Part:BBa_K4103009 | |||
| http://parts.igem.org/Part:BBa_K4103016 | OriTBsub | ||
| http://parts.igem.org/Part:BBa_K4103017 | Terminator | E. coli terminator | 54 |
| http://parts.igem.org/Part:BBa_K4103018 | Promoter | kanR promoter | 101 |
| http://parts.igem.org/Part:BBa_K4103019 | Terminator | kanR terminator | 48 |
| http://parts.igem.org/Part:BBa_K4103020 | Coding | kanR cds | 816 |
| http://parts.igem.org/Part:BBa_K4103021 | Coding | lacI cds | 1083 |
| http://parts.igem.org/Part:BBa_K4103022 | |||
| http://parts.igem.org/Part:BBa_K4103023 | Operator | Lac operator | 25 |
| http://parts.igem.org/Part:BBa_K4103024 | Insulator | RiboJ54 Insulator | 75 |
| http://parts.igem.org/Part:BBa_K4103025 | |||
| http://parts.igem.org/Part:BBa_K4103026 | Coding | Pfu (DNA polymerase) #4 | 2322 |
| http://parts.igem.org/Part:BBa_K4103027 | Coding | Sso-7d #4 | 189 |
| http://parts.igem.org/Part:BBa_K4103028 | Linker | TEV linker site #4 | 21 |
| http://parts.igem.org/Part:BBa_K4103029 | Coding | fuGFP #4 | 732 |
| http://parts.igem.org/Part:BBa_K4103030 | Tag | 10xHisTag #4 | 30 |
| http://parts.igem.org/Part:BBa_K4103031 | Tag | R5 Silica binding purification tag #4 | 57 |
| http://parts.igem.org/Part:BBa_K4103032 | Coding | Pfu (DNA polymerase) #2 | 2322 |
| http://parts.igem.org/Part:BBa_K4103033 | Coding | Sso-7d #2 | 189 |
| http://parts.igem.org/Part:BBa_K4103034 | Linker | TEV linker site | 21 |
| http://parts.igem.org/Part:BBa_K4103035 | Coding | fuGFP | 732 |
| http://parts.igem.org/Part:BBa_K4103036 | Purification Tag | 10xHisTag | 30 |
| http://parts.igem.org/Part:BBa_K4103037 | Purification Tag | R5 Silica binding purification tag | 57 |
| http://parts.igem.org/Part:BBa_K4103038 | Coding | ccdb | 335 |
| http://parts.igem.org/Part:BBa_K4103039 | Homology Arm | lacA 3' homology | 408 |
| http://parts.igem.org/Part:BBa_K4103040 | Homology Arm | amyE 5' homology | 521 |
| http://parts.igem.org/Part:BBa_K4103041 | Homology Arm | amyE 3' homology | 498 |
| http://parts.igem.org/Part:BBa_K4103042 | Homology Arm | thrC 5' homology | 501 |
| http://parts.igem.org/Part:BBa_K4103043 | Homology Arm | thrC 3' homology | 429 |
| http://parts.igem.org/Part:BBa_K4103044/ | Homology Arm | lacA 5' homology arm | 602 |
| http://parts.igem.org/Part:BBa_K4103045 | Secretion Tag | AmyQ | 93 |
Composite Parts
| Part Name | Type | Description | Length (bp) |
|---|---|---|---|
| http://parts.igem.org/Part:BBa_K4103000 | pfu | ||
| http://parts.igem.org/Part:BBa_K4103001 | Conjugation origin | Transfer Origin pIP501 | 319 |
| http://parts.igem.org/Part:BBa_K4103003 | Resistance marker | SpecR | 989 |
| http://parts.igem.org/Part:BBa_K4103004 | Origin of replication | EcoORI | 589 |
| http://parts.igem.org/Part:BBa_K4103005 | Origin of transfer | OriTBsub | 378 |
| http://parts.igem.org/Part:BBa_K4103007 | Resistance marker | catA9 | 939 |
| http://parts.igem.org/Part:BBa_K4103008 | Insulator | HDV-1 (3' Ribozyme Insulator) | 85 |
| http://parts.igem.org/Part:BBa_K4103009 | |||
| http://parts.igem.org/Part:BBa_K4103016 | OriTBsub | ||
| http://parts.igem.org/Part:BBa_K4103017 | Terminator | E. coli terminator | 54 |
| http://parts.igem.org/Part:BBa_K4103018 | Promoter | kanR promoter | 101 |
| http://parts.igem.org/Part:BBa_K4103019 | Terminator | kanR terminator | 48 |
| http://parts.igem.org/Part:BBa_K4103020 | Coding | kanR cds | 816 |
| http://parts.igem.org/Part:BBa_K4103021 | Coding | lacI cds | 1083 |
| http://parts.igem.org/Part:BBa_K4103022 | |||
| http://parts.igem.org/Part:BBa_K4103023 | Operator | Lac operator | 25 |
| http://parts.igem.org/Part:BBa_K4103024 | Insulator | RiboJ54 Insulator | 75 |
| http://parts.igem.org/Part:BBa_K4103025 | |||
| http://parts.igem.org/Part:BBa_K4103026 | Coding | Pfu (DNA polymerase) #4 | 2322 |
| http://parts.igem.org/Part:BBa_K4103027 | Coding | Sso-7d #4 | 189 |
| http://parts.igem.org/Part:BBa_K4103028 | Linker | TEV linker site #4 | 21 |
| http://parts.igem.org/Part:BBa_K4103029 | Coding | fuGFP #4 | 732 |
| http://parts.igem.org/Part:BBa_K4103030 | Tag | 10xHisTag #4 | 30 |
| http://parts.igem.org/Part:BBa_K4103031 | Tag | R5 Silica binding purification tag #4 | 57 |
| http://parts.igem.org/Part:BBa_K4103032 | Coding | Pfu (DNA polymerase) #2 | 2322 |
| http://parts.igem.org/Part:BBa_K4103033 | Coding | Sso-7d #2 | 189 |
| http://parts.igem.org/Part:BBa_K4103034 | Linker | TEV linker site | 21 |
| http://parts.igem.org/Part:BBa_K4103035 | Coding | fuGFP | 732 |
| http://parts.igem.org/Part:BBa_K4103036 | Purification Tag | 10xHisTag | 30 |
| http://parts.igem.org/Part:BBa_K4103037 | Purification Tag | R5 Silica binding purification tag | 57 |
| http://parts.igem.org/Part:BBa_K4103038 | Coding | ccdb | 335 |
| http://parts.igem.org/Part:BBa_K4103039 | Homology Arm | lacA 3' homology | 408 |
| http://parts.igem.org/Part:BBa_K4103040 | Homology Arm | amyE 5' homology | 521 |
| http://parts.igem.org/Part:BBa_K4103041 | Homology Arm | amyE 3' homology | 498 |
| http://parts.igem.org/Part:BBa_K4103042 | Homology Arm | thrC 5' homology | 501 |
| http://parts.igem.org/Part:BBa_K4103043 | Homology Arm | thrC 3' homology | 429 |
| http://parts.igem.org/Part:BBa_K4103044/ | Homology Arm | lacA 5' homology arm | 602 |
| http://parts.igem.org/Part:BBa_K4103045 | Secretion Tag | AmyQ | 93 |