Difference between revisions of "Team:ASU/Results"

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<h1>Results</h1>
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<p>You can describe the results of your project and your future plans here. </p>
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<h3>What should this page contain?</h3>
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<li> Clearly and objectively describe the results of your work.</li>
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<li> Future plans for the project. </li>
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<li> Considerations for replicating the experiments. </li>
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<h3>Describe what your results mean </h3>
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<li> Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for. </li>
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<li> Show data, but remember <b>all measurement and characterization data must also be on the Part's Main Page on the <a href="http://parts.igem.org/Main_Page">Registry</a>.</b> Otherwise these data will not be in consideration for any medals or part awards! </li>
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<li> Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project. </li>
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<h3> Project Achievements </h3>
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<p>You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.</p>
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<li>A list of linked bullet points of the successful results during your project</li>
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<li>A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.</li>
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<title>Contribution Page</title>
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<h3>Inspiration</h3>
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<p>See how other teams presented their results.</p>
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<li><a href="https://2019.igem.org/Team:Newcastle/Results">2019 Newcastle</a></li>
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<li><a href="https://2019.igem.org/Team:Munich/Results">2019 Munich </a></li>
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<li><a href="https://2019.igem.org/Team:Tec-Chihuahua/Results">2019 Tec Chihuahua</a></li>
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<li><a href="https://2020.igem.org/Team:Aalto-Helsinki/Results">2020 Aalto Helsinki</a></li>
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<li><a href="https://2020.igem.org/Team:GreatBay_SCIE/Results">2020 GreatBay SCIE</a></li>
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<li><a href="https://2020.igem.org/Team:Queens_Canada/Results">2020 Queens Canada</a></li>
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Results
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<div class="header-text">cloning results</div>
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MT:
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Metallothionein (MT) was introduced into our plasmid backbone pASapI via Gibson assembly. This sequence was designed specifically with two type IIS restriction sites for SapI on either side of the MT sequence and ordered from IDT. This allowed us to utilize the MT plasmid as the backbone for all consecutive cloning and enabled us to proceed with golden gate assembly in the future. MT was cloned into 5-alpha competent e.coli cells from NEB. The transformation was successful, as the positive control plate for MT had significantly more colonies than the negative control plate. This cloning was confirmed via restriction digest using XbaI and BstxI. During the Gibson assembly, we excised the NcoI-HindIII linker in the pASapI backbone which contained an XbaI site. Therefore, the restriction digests of MT should only yield two bands (4.4kb, 2.4 kb) instead of the three bands from pASapI (4.4kb, 1.4kb, 0.8kb). The presence of only two bands, the increased size of the second band, indicated that MT was successfully cloned into the plasmid. For all future experiments, this plasmid was used as the backbone, known as MT-pASapI.
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<div class="header-text">creation of new parts</div>
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<div class="header-text">methods of transformation</div>
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Revision as of 22:56, 21 October 2021

Contribution Page
Results
cloning results
MT: Metallothionein (MT) was introduced into our plasmid backbone pASapI via Gibson assembly. This sequence was designed specifically with two type IIS restriction sites for SapI on either side of the MT sequence and ordered from IDT. This allowed us to utilize the MT plasmid as the backbone for all consecutive cloning and enabled us to proceed with golden gate assembly in the future. MT was cloned into 5-alpha competent e.coli cells from NEB. The transformation was successful, as the positive control plate for MT had significantly more colonies than the negative control plate. This cloning was confirmed via restriction digest using XbaI and BstxI. During the Gibson assembly, we excised the NcoI-HindIII linker in the pASapI backbone which contained an XbaI site. Therefore, the restriction digests of MT should only yield two bands (4.4kb, 2.4 kb) instead of the three bands from pASapI (4.4kb, 1.4kb, 0.8kb). The presence of only two bands, the increased size of the second band, indicated that MT was successfully cloned into the plasmid. For all future experiments, this plasmid was used as the backbone, known as MT-pASapI.

creation of new parts
methods of transformation