Difference between revisions of "Team:SHSID/Contribution"

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                    <a href="https://2021.igem.org/Team:SHSID">Home</a>
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                    <a href="https://2021.igem.org/Team:SHSID/Implementation">Implementation</a>
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                    <a href="https://2021.igem.org/Team:SHSID/Model">Model</a>
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                                    Members</a></li>
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                                    class="sub-nav-74">Attributions</a></li>
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                            <li><a href="https://2021.igem.org/Team:SHSID/Collaborations"
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    <div class="sub-content">
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        <div class="sub-title">Contribution</div>
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        <div class="article-title">BBa_K3993000 </div>
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        <div class="article-content"><b>== Profile ==</b></div>
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        <div class="article-content">
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            Name: SPE1 <br />
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            Base Pairs: 1401bp<br />
 +
            Origin: Saccharomyces cerevisiae, genome<br />
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            Properties: Catalyzes the first and rate-limiting step of polyamine biosynthesis that converts ornithine
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            into putrescine
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        </div>
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        <div class="article-content"><b>==== Usage and Biology ====</b></div>
 +
        <div class="article-content">his protein is involved in step 1 of the subpathway that synthesizes putrescine
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            from L-ornithine. Catalyzes the first and rate-limiting step of polyamine biosynthesis that converts
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            ornithine into putrescine, which is the precursor for the polyamines, spermidine and spermine. Polyamines
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            are essential for cell proliferation and are implicated in cellular processes, ranging from DNA replication
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            to apoptosis. Homodimer and only the dimer is catalytically active, as the active sites are constructed of
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            residues from both monomers </div>
 +
        <div class="img-wrap no-margin">
 +
            <img src="https://static.igem.org/mediawiki/2021/7/7f/T--SHSID--contribution01.jpg" alt="" />
 +
            <span>Figure1. Principle diagram of TAs.</span>
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        </div>
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        <div class="article-title">BBa_K3993001</div>
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        <div class="article-content"><b>== Profile ==</b></div>
 +
        <div class="article-content">
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            Name: SPEB<br />
 +
            Base Pairs: 921bp<br />
 +
            Origin: E. coli, genome<br />
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            Properties: Catalyzes the formation of putrescine from agmatine.
 +
        </div>
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        <div class="article-title">BBa_K3993002</div>
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        <div class="article-content"><b>== Profile ==</b></div>
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        <div class="article-content">
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            Name: AsADC<br />
 +
            Base Pairs: 1821bp<br />
 +
            Origin: Saccharomyces cerevisiae, synthesis<br />
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            Properties: codon optimized SPE1
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        </div>
 +
        <div class="article-title">BBa_K3993010</div>
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        <div class="article-content"><b>== Profile ==</b></div>
 +
        <div class="article-content">
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            Name: PTDH3-SPE1-TCYC1<br />
 +
            Base Pairs: 2316bp<br />
 +
            Origin: Saccharomyces cerevisiae, E. coli, synthesis<br />
 +
            Properties: Arginine metabolism and polyamine biosynthesis chemical reactions
 +
        </div>
 +
        <div class="article-content"><b>== Construct design ==</b></div>
 +
        <div class="article-content">The Tropine part of Tropane alkaloids (TAs) is obtained from arginine to putrescine
 +
            (1,4-butanediamine, putrescine), and then through a series of chemical reactions. In this project, natural
 +
            genes involved in arginine metabolism and polyamine biosynthesis was designed to overexpress in yeast. The
 +
            engineer strains that produced excess putrescine. (Figure 2).</div>
 +
        <div class="img-wrap no-margin">
 +
            <img src="https://static.igem.org/mediawiki/2021/5/53/T--SHSID--contribution02.jpg" alt="" />
 +
            <span>Figure 2. DNA sequence map of plasmid PTDH3-SPE1-TCYC1.</span>
 +
        </div>
 +
        <div class="article-title">BBa_K3993011</div>
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        <div class="article-content"><b>== Profile ==</b></div>
 +
        <div class="article-content">
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            Name: PTDH3-speB-TCYC1<br />
 +
            Base Pairs: 1836bp<br />
 +
            Origin: Saccharomyces cerevisiae, E. coli, synthesis<br />
 +
            Properties: Arginine metabolism and polyamine biosynthesis chemical reactions
 +
        </div>
 +
        <div class="article-content"><b>== Construct design ==</b></div>
 +
        <div class="article-content">The Tropine part of Tropane alkaloids (TAs) is obtained from arginine to putrescine
 +
            (1,4-butanediamine, putrescine), and then through a series of chemical reactions. In this project, natural
 +
            genes involved in arginine metabolism and polyamine biosynthesis was designed to overexpress in yeast. The
 +
            engineer strains that produced excess putrescine. (Figure 2).</div>
 +
        <div class="img-wrap no-margin">
 +
            <img src="https://static.igem.org/mediawiki/2021/7/7f/T--SHSID--contribution03.jpg" alt="" />
 +
            <span>Figure 2. DNA sequence map of plasmid PTDH3-SpeB-TCYC1.</span>
 +
        </div>
 +
        <div class="article-title">BBa_K3993012</div>
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        <div class="article-content"><b>== Profile ==</b></div>
 +
        <div class="article-content">
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            Name: PTDH3-AsADC-TCYC1<br />
 +
            Base Pairs: 2736bp<br />
 +
            Origin: Saccharomyces cerevisiae, E. coli, synthesis<br />
 +
            Properties: Arginine metabolism and polyamine biosynthesis chemical reactions
 +
        </div>
 +
        <div class="article-content"><b>== Construct design ==</b></div>
 +
        <div class="article-content">The Tropine part of Tropane alkaloids (TAs) is obtained from arginine to putrescine
 +
            (1,4-butanediamine, putrescine), and then through a series of chemical reactions. In this project, natural
 +
            genes involved in arginine metabolism and polyamine biosynthesis was designed to overexpress in yeast. The
 +
            engineer strains that produced excess putrescine. (Figure 2).</div>
 +
        <div class="img-wrap no-margin">
 +
            <img src="https://static.igem.org/mediawiki/2021/6/6f/T--SHSID--contribution04.jpg" alt="" />
 +
            <span>Figure 2. DNA sequence map of plasmid PTDH3-AsADC-TCYC1.</span>
 +
        </div>
 +
        <div class="article-title">BBa_K3993013</div>
 +
        <div class="article-content"><b>== Profile ==</b></div>
 +
        <div class="article-content">
 +
            Name: PPGK1-AsADC-TADH1 - PTEF1-SPE1-TTEF1 - PTDH3-speB-TCYC1<br />
 +
            Base Pairs: 6866 bp<br />
 +
            Origin: Saccharomyces cerevisiae, E. coli, synthesis, genome<br />
 +
            Properties: Arginine metabolism and polyamine biosynthesis chemical reactions
 +
        </div>
 +
        <div class="article-content"><b>== Construct design ==</b></div>
 +
        <div class="article-content">The Tropine part of Tropane alkaloids (TAs) is obtained from arginine to putrescine
 +
            (1,4-butanediamine, putrescine), and then through a series of chemical reactions. In this project, natural
 +
            genes involved in arginine metabolism and polyamine biosynthesis was designed to overexpress in yeast. The
 +
            engineer strains that produced excess putrescine. (Figure 2). </div>
 +
        <div class="img-wrap no-margin">
 +
            <img src="https://static.igem.org/mediawiki/2021/f/f1/T--SHSID--contribution05.jpg" alt="" />
 +
            <span>Figure 2. DNA sequence map of plasmid pYES2-AsADC-SPE1-SpeB.</span>
 +
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 +
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<h1>Contribution </h1>
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<H3>
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Bronze Medal Criterion #4
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</H3>
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<p>Make a useful contribution for future iGEM teams. Use this page to document that contribution.
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<br><br>
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Please see the <a href="https://2021.igem.org/Judging/Medals">2021 Medals Page</a> for more information.
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<p>If you are making a contribution by adding information to an existing Part or creating a new Part, you must document your contribution on the Part's Main Page on the <a href="http://parts.igem.org/Main_Page">Registry</a> for your team to be eligible for this criteria. You can use this page to link to that part and include additional information about your contribution.</p>
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Latest revision as of 16:49, 19 October 2021

SHSID

Contribution
BBa_K3993000
== Profile ==
Name: SPE1
Base Pairs: 1401bp
Origin: Saccharomyces cerevisiae, genome
Properties: Catalyzes the first and rate-limiting step of polyamine biosynthesis that converts ornithine into putrescine
==== Usage and Biology ====
his protein is involved in step 1 of the subpathway that synthesizes putrescine from L-ornithine. Catalyzes the first and rate-limiting step of polyamine biosynthesis that converts ornithine into putrescine, which is the precursor for the polyamines, spermidine and spermine. Polyamines are essential for cell proliferation and are implicated in cellular processes, ranging from DNA replication to apoptosis. Homodimer and only the dimer is catalytically active, as the active sites are constructed of residues from both monomers
Figure1. Principle diagram of TAs.
BBa_K3993001
== Profile ==
Name: SPEB
Base Pairs: 921bp
Origin: E. coli, genome
Properties: Catalyzes the formation of putrescine from agmatine.
BBa_K3993002
== Profile ==
Name: AsADC
Base Pairs: 1821bp
Origin: Saccharomyces cerevisiae, synthesis
Properties: codon optimized SPE1
BBa_K3993010
== Profile ==
Name: PTDH3-SPE1-TCYC1
Base Pairs: 2316bp
Origin: Saccharomyces cerevisiae, E. coli, synthesis
Properties: Arginine metabolism and polyamine biosynthesis chemical reactions
== Construct design ==
The Tropine part of Tropane alkaloids (TAs) is obtained from arginine to putrescine (1,4-butanediamine, putrescine), and then through a series of chemical reactions. In this project, natural genes involved in arginine metabolism and polyamine biosynthesis was designed to overexpress in yeast. The engineer strains that produced excess putrescine. (Figure 2).
Figure 2. DNA sequence map of plasmid PTDH3-SPE1-TCYC1.
BBa_K3993011
== Profile ==
Name: PTDH3-speB-TCYC1
Base Pairs: 1836bp
Origin: Saccharomyces cerevisiae, E. coli, synthesis
Properties: Arginine metabolism and polyamine biosynthesis chemical reactions
== Construct design ==
The Tropine part of Tropane alkaloids (TAs) is obtained from arginine to putrescine (1,4-butanediamine, putrescine), and then through a series of chemical reactions. In this project, natural genes involved in arginine metabolism and polyamine biosynthesis was designed to overexpress in yeast. The engineer strains that produced excess putrescine. (Figure 2).
Figure 2. DNA sequence map of plasmid PTDH3-SpeB-TCYC1.
BBa_K3993012
== Profile ==
Name: PTDH3-AsADC-TCYC1
Base Pairs: 2736bp
Origin: Saccharomyces cerevisiae, E. coli, synthesis
Properties: Arginine metabolism and polyamine biosynthesis chemical reactions
== Construct design ==
The Tropine part of Tropane alkaloids (TAs) is obtained from arginine to putrescine (1,4-butanediamine, putrescine), and then through a series of chemical reactions. In this project, natural genes involved in arginine metabolism and polyamine biosynthesis was designed to overexpress in yeast. The engineer strains that produced excess putrescine. (Figure 2).
Figure 2. DNA sequence map of plasmid PTDH3-AsADC-TCYC1.
BBa_K3993013
== Profile ==
Name: PPGK1-AsADC-TADH1 - PTEF1-SPE1-TTEF1 - PTDH3-speB-TCYC1
Base Pairs: 6866 bp
Origin: Saccharomyces cerevisiae, E. coli, synthesis, genome
Properties: Arginine metabolism and polyamine biosynthesis chemical reactions
== Construct design ==
The Tropine part of Tropane alkaloids (TAs) is obtained from arginine to putrescine (1,4-butanediamine, putrescine), and then through a series of chemical reactions. In this project, natural genes involved in arginine metabolism and polyamine biosynthesis was designed to overexpress in yeast. The engineer strains that produced excess putrescine. (Figure 2).
Figure 2. DNA sequence map of plasmid pYES2-AsADC-SPE1-SpeB.
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