Team:Yucai SZ/Part Collection

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New Part

Basic Parts

This year, we submitted a new part for the silver medal. You can click on the part number to see the details.

Part Number	Name	Part Description
BBa_K4055517	Mfp3S-pep	Mfp3s—pep is a kind of Mussel foot protein peptide.Mfp3S-pep is enriched with aspartic acid (blue), lysine (green) and tyrosine (red) residues, known for their functional roles in promoting the nucleation, growth and adhesion of HA.
BBa_K4055521	CsgA segment	Major Curlin Subunit Precursor from Escherichia coli K12(CsgA) This part is used to express csgA protein which are bio-inspired adhesive peptides. CsgA protein is the main protein component of the biofilm and contributes to the adhesion of the biofilm to solid surfaces. It helps the cell stick to various surfaces.

Composite Parts

These Composite Parts is composed of some basic parts are assembled together with promoter, ribosome binding site or terminator, which are presented in the following table. You can click on the part number to see the details.

Part Number	Name	Part Description
BBa_K4055001	Photosensitive T7RNAP	This split T7RNAP has little activity in dark environment. When the two segments were exposed to blue light, they will bind together due to the photodimerization of VVD domain and exhibit full RNA polymerase activity. Acturally, this part was designed by last year's team, SMS_Shenzhen, but had not been tested and regestered. After obtaining their consent, we tested and registered this year. It also can be used for constructing a photo sensor system with T7 promoter.
BBa_K4055532	pT7-B0034-amilCP	Expression of amilCP pigment protein is performed using the T7 promoter, which is used to test our photo sensor devices(for example, BBa_K4055001).
BBa_K4055533	CsgA-Mfp3S-pep	Biofilm structural proteins in E. coli fused with mussel foot protein(Mfp) analogs bestowed the engineered biofilms with HA mineralization-promoting and interfacial binding roles.
BBa_K4055531	pT7-B0034-CsgA-Mfp3S-pep	Biofilm structural proteins in E. coli fused with mussel foot protein(Mfp) analogs bestowed the engineered biofilms with HA mineralization-promoting and interfacial binding roles. When a blue light-induced strain was used to grow functional biofilms with controlled spatial and biomass density, living mineralized materials with patterning and gradient features could be generated following a benign biomimetic HA mineralization process.

Improved Parts

This year, our team has improved the existing parts.The improved parts and original existing parts are presented below. You can click on the part number to see the details.

Part Number	Name	Part Description	Improvement
BBa_K4055525	pT7-PhlO	A modified T7 phage promoter which can be regulated by the repressor PhlF	Compared with the original(BBa_J64997), this design allows the T7 promoter can be tightly regulated by PhlF.