Team:XHD-Wuhan-Pro-China/Collaborations
Collaboration
Our XHD-Wuhan-Pro-China team has been collaborating regularly with other iGEM teams throughout the preparation for 2021. We have established a good cooperative relationship with the team from Wuhan University, the team from Xi'an Jiaotong-Liverpool University, the team from Shanghai Tech University, the XHD-Wuhan-A-China team, the XHD-Wuhan-B-China team, the LZU-HS-China team, and the Thinker-EDU-China team. We have many forms of collaboration with other teams, including meet-ups, lab collaboration, project communication and so on. These inter-provincial partnerships have helped us, and other teams better complete our projects.
Collaboration with WHU-China (2021.7.25)
Their wiki link : https://2021.igem.org/Team:WHU-China/Collaborations
On July 25, 2021, we went to Wuhan University to communicate with a team of college students. During this communication, we had the opportunity to introduce our program to the iGEM team at Wuhan University and get information about their program.Since WHU-China’s project is to treat acne with genetically engineered bacteria, they also introduced some basic knowledge about acne itself, in addition to their project design. We have learnt a lot from this introduction.Subsequently, students from the Wuhan University team raised questions about the folding of eukaryotic genes in prokaryotes for our project, which may need further exploration. They told us that the ADH gene comes from yeast, a eukaryote, so when expressed in bacteria it can be misfolded and form precipitation. The question got us thinking and we finally came up with a solution. We added a SUMO tag for modification so that the ADH gene could be expressed in a more tolerable form. To sum up, thanks to the friendly communication between our two teams, they were able to raise questions about our project, and we finally found a solution to fix this problem. Just because of this communication, we saw the way and depth of college students' thinking, so we further optimized our own project.
Collaboration with Thinker-EDU-China & Collaboration with LZU-HS-China (2021.7.28) < a href="2021.igem.org/Team:Thinker-Edu-China/Collaborations"> < a href="2021.igem.org/Team:LZU-HS-China/Collaborations">
On July 28, 2021, our team had friendly communication with two high-school teams, Thinker-EDU-China and LZU-HS-China. They are also the team that we have partnership with.
1. We recommended E.coli Nissle 1917 as the chassis creature to LZU-HS-China whose goal was also to construct an intestinal probiotic due to the high safety and highly available genetic background of this bacteria.
2. We discussed the activity of conducting expert interviews and hospital field investigations together. After the discussion, we decided to go to the hospital together for an interview, and we also discussed the question design and the contents of the hospital investigation.
3. Members from LZU-HS-China guided us to create short animation clips for our promotion video using Adobe Aftereffects and Adobe Premiere. This helped us to clearly demonstrate the process of alcohol digestion and the functions of our product.
4. We decided to help promote each other's projects in our own schools, so as to jointly promote the development and communication of each other's projects.
5. Members from LZU-HS-China discovered that because EcN contains secret plasmids, it can interfere with the expression of foreign genes at specific locations to some extent and disturbed the efficacy of the gene and protein. Thus, they ameliorated their project by adopting a new high copy plasmid-pSB1A3. Moreover, the strong promoter before the expressive sequence and INP, an appropraite and stable protein transporter binding on cell membrane, were added in front of the target gene to promote the efficiency and stability.LZU-HS-China shared these achievements with us and helped us avoid potential deficiencies in our experiments.
Collaboration with XHD-Wuhan-A-China & XHD-Wuhan-B-China (2021.7.30) < a href="2021.igem.org/Team:XHD-Wuhan-A-China/Collaborations"> < a href="2021.igem.org/Team:XHD-Wuhan-B-China/Collaborations">
On July 30, 2021, our team communicated with the XHD-Wuhan-A-China team and the XHD-Wuhan-B-China team, which are two high school teams. First of all, we introduced our own projects to each other and listened to the other two teams talking about their projects.
With XHD-Wuhan-A-China
After the introduction, the students of the XHD-Wuhan-A-China team thought that we need animal experiments because our project works in the small intestine of human. In addition, some consumers may object to animal experiments. For this question, we gave our explanations. In fact, the small intestines in which our probiotics compete for alcohol to degrade still belongs to the external environment. There is no need to take the complex internal metabolism into account, so animal experiment is unnecessary. The result would be same whether in lab or the human body. The questions raised by the XHD-Wuhan-A-China team let us find a point that might be misunderstood by the audience so that we would emphasize and explain it more clearly in our speech in the future.
With XHD-Wuhan-B-China
The project topic of the XHD-Wuhan-B-China team was relatively complex and unpopular, which might not be put into large scale practice in the near future, so they consulted us on how to publicize their project. We proposed to show the broad application prospect of this project and tell people the benefits that could be brought by its application so that people can be willing to support their project and have full expectations for.
Collaboration with XJTLU-CHINA(2021.8.16)
< a href="2021.igem.org/Team:XJTLU-CHINA/Collaborations">On 16 August 2021, we met with the iGEM team from Xi'an Jiaotong-Liverpool University. We introduced our projects to each other.
1.We decided to help each other to promote their projects on our official accounts, online article, and in school speeches.
2.Through communication, we found that the projects of our two teams are all related to food, so the team from Xi'an Jiaotong-Liverpool University said that when they went to interview the Market Supervision Administration, they could ask some of the questions we raised and give us the response from Market Supervision Administration.
3.Their team also recommended the modeling software MATLAB to us, which is very helpful.
Collaboration with ShanghaiTech_China (2021.8.16)
On August 16, 2021, members of the iGEM team from Shanghai Tech University explained and guided us in modeling. First, they introduced us to the modeling part in their project. Then, they started to explain several equations often used in modeling in detail such as the ordinary differential equations. In addition, they proposed several starting points in modeling for our project, which gave us some inspiration.
