Team:Virginia/Protocols
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Protocols
Index
Protocols
Introduction
The following files are instructions for creating parts and experimenting for our project MANIFOLD . A more indepth explanation of procedures can be found on our Experiments page under the Project tab.
Protocol Files
8_26-8_28
Addgene Gel and BBA inoculations 9_29
Agenda for 10_2
BBA 10_7
Diagnostic Digest—general protocol
Inoculate a Bacterial Culture (Addgene)
Miniprep protocol
New Transformation Protocol with 10-B Competent Cells
Oct 18
Protocol 9_14_21
Transformation NEB Golden Gate Assembly Kit (BsaI-HFv2)
Transformation Protocol using NEB 10-beta Competent E. coli
Western Blot Protocol
Competent Cells Preparation
DNA Kit Plate Instructions (iGEM)
Golden Gate Assembly for Using NEB Golden Gate Assembly Kit (BsaI-HFv2)
PCR Purification (Qiagen)
Qiagen Miniprep (Centrifuge)- pg 26
Addgene Gel and BBA inoculations 9_29
Agenda for 10_2
BBA 10_7
Diagnostic Digest—general protocol
Inoculate a Bacterial Culture (Addgene)
Miniprep protocol
New Transformation Protocol with 10-B Competent Cells
Oct 18
Protocol 9_14_21
Transformation NEB Golden Gate Assembly Kit (BsaI-HFv2)
Transformation Protocol using NEB 10-beta Competent E. coli
Western Blot Protocol
Competent Cells Preparation
DNA Kit Plate Instructions (iGEM)
Golden Gate Assembly for Using NEB Golden Gate Assembly Kit (BsaI-HFv2)
PCR Purification (Qiagen)
Qiagen Miniprep (Centrifuge)- pg 26
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485 McCormick Rd
CHARLOTTESVILLE, VA 22903
485 McCormick Rd
CHARLOTTESVILLE, VA 22903
