Home Team Team Attributions Collaborations Project Description Design Proof of concept Engineering Results Notebook Implementation Contribution Experiments Parts Safety Human Human Practices Communication Partnership Jamboree Organization Awards Education Model Sustainable Notebook Writing the project down From March to October we have developed the amazing project Reconby, many weeks of hard work, long nigths and lots of fun. Here we have summarized the different activities we have done during this time and how we organized our work through these months! Week 1 (March 1 to 7) After being chosen as members of the first UNIZAR iGEM team, we had our first online meeting and finally got to know each other in-person. Week 2 (March 7 to 14) We started working on the roster reveal for social media and some team members were officially announced on Twitter, Instagram and Facebook. Week 3 (March 15 to 21) We had our first project brainstorming meeting and initial task distribution. As well as, we recorded and edited our presentation videos. Week 4 (March 22 to 28) Proposed projects selection of final candidates. We chose four final candidates to focus our research. Week 5 (March 29 to April 4) Easter Holidays. Week 6 (April 5 to 11) Definitive project selection after a long online meeting debating pros and cons. Week 7 (April 12 to 18) We focused our research on defining more specific details of our final project. We increased our search for funding efforts contacting with some of the most important local companies and institutions related to the biological industry. Week 8 (April 19 to 25) We consulted Luis Alberto Anel professor of Biochemistry and Molecular and Cellular Biology at the University of Zaragoza, who recommended us to contact Dr. Laura Sanz, an expert in recombinant nanobodies. Week 9 (April 26 to May 2) We had our first "iberian meeting" with other Spanish iGEM teams. It was our first opportunity to present our projects to each other and to exchange our views on the iGEM competition and some of the problems we had encountered so far. Week 10 (May 3 to 9) We continued developing a more detailed guideline of our wet lab project as we kept looking for funding. We held our first online meeting with Operon and other companies. Week 11 (May 10 to 16) University of Zaragoza's student Vice-Rectorate office agree to pay the entire registration fee. However we kept working on finding more sponsors to cover other fees and laboratory materials and reagents. Week 12 (May 17 to 23) We kept working in our exciting project while we were looking for more sponsors. Week 13 (May 24 to 30) After weeks of reaseach and taking to experts in recombinant nanobodies such as Dr. Laura Sanz we got to the conclusion that our original project was unfeasible. However, we found a way to adapt our project design towards a more realistic approach, maintaining its backbone: creating a library of artificially developed nanobodies obtained by random in vitro recombination of the different CDR genetic sequences of already characterized nanobodies, and express them in E. coli. Moreover, we registerde our inscription and officially became part of the iGEM competition. Week 14 (May 31 to June 6), Week 15 (June 7 to June 13 ), Week 16 ( June 13 to 20) We were taking our final exams, therefore we stopped iGEM activity for two weeks. Week 17 (June 21 to 27) After our exams, we started planning our wet lab and other issues such as our education activities. In addition, our plasmids arrived at our lab. Week 18 (June 28 to July 4) Diego, Olga, Carla and Alba, responsible for web development, met up to decide some of the basic feature of our future web such as format, form or colours. In addition, Diego, Ainhoa and Alba Pueyo had a dry lab meeting to check our primers design and sequences allingments and changes. Week 19 (July 5 to 11) We arranged a meeting with SynBio Amazonas a group of graduated and undergraduated scientist who also worked with nanobodies and had participated in the iGEM competition. Week 20 (July 12 to 18) As a iGEM requirement, we recorded the presentation video Victor edited. Week 21 (July 19 to 25) In July 20th, we took our biosafety and biosecurity in orde to improve safety in our project. Moreover, responsibles for web development had a workshop about wiki design with the 2020 UPF team. Week 22 (July 26 to August 1) An article about our project was published at "Heraldo de Aragón", Aragon's most well-known regional newspaper. We as well created a survey to get feedback from overall population and we went out to the streets of Zaragoza to interview people who passed by. Week 23 (August 2 to 8) In August 7th, we installed two communication stands in two Aragonese capital cities, Teruel and Huesca. Afterwards, "Eco Teruel" published an article about us. Week 24 (August 9 to 15) During a long meeting, our team divided tasks while we were planning to start our wetlab. Week 25 (August 16 to 22) We were told that we would be able to start our lab work next week so we had a meeting to define tasks distribution and each team member assignments. Week 26 (August 23 to 29) Finally, we started our wetlab work! Materials and reagents finally arrived and we were ready to get started in the lab. We resuspended our primers and started to test materials and kits. Week 27 (August 30 to September 5) We placed another stand with experiments in another town in our region: Calatayud. Week 28 (September 6 to 12) Concurrently to our wetlab work, we did a survey data analysis. Week 29 (September 13 to 19) We had a meeting with Beatriz Latre to improve our science communication and put in practice what we had learnt in the next experiments stand we placed in our fourth destination: Monzón, Huesca, Zaragoza and Teruel. Week 30 (September 20 to 26) We participated in the European Researchers' Night in Zaragoza. As well, we placed a final science communication stand in our city centre. We had human practices meeting with Dr. Esteban Martínez and Dr. Rosa Monge. Week 31 (September 27 to October 3) With education activities finished, we kept our focus on wetlab work. We also filled and sent the Safety Form and the preliminary Judging Form. We had human practices meeting with Dr. Susana Gamen. Week 32 (October 4 to October 10) We tried to make the most of our finals days in the lab and reinforced our wiki editing. Alba Pueyo wrote the scaffold of the Team Presentation Video script. Week 33 (October 11 to October 17) Wiki text writing and checking and wiki editing. Week 34 (October 18 to October 24) Final wiki editing
