24.02.2021: First team meeting: Organisation and brainstorming for project ideas
14.03.2021: 2nd meeting: Exchange about project and researching ideas. Topics: Different synthetic foods (milk and egg production) or fungus sensors
19.03.2021: 3rd meeting: Agreement on a project - production of platypus yogurt, creation of subteams
23.03.2021: 4th meeting: Researching on milk ingredients and their production, finding a PI and financial resources (sponsors)
02.04.2021: 5th meeting: Further planning. Interim status exchange in all subgroups
11.04.2021: 6th meeting: Information exchange
18.04.2021: 7th meeting: Information exchange
25.04.2021: 8th meeting: Information exchange and writing of articles, creating a web page
30.04.2021: 9th meeting: Information exchange
05.05.2021: First mentor meeting
07.05.2021: 10th meeting: Information exchange. From now on 3 regular meetings per week.
14.05.2021: German meet-up, 11th meeting
17.05.2021: First meeting with our PI, Prof. Dr. Dirk Tischler. Laboratory work is guaranteed, starting mid July
19.05.2021: 2nd mentor meeting
21.05.2021: 12th meeting: Information exchange
25.05.2021: First meeting with team Darmstadt: Information exchange and help
28.05.2021: 13th meeting: Information exchange
29.05.2021: RUBochum Game night
02.06.2021: 3rd mentor meeting
04.06.2021: 14th meeting: Information exchange and introduction into methods of bioinformatics
05.07.2021: Start of laboratory work; continues every day until 07.10.2021
07.06.2021: Development meeting for primers and bioinformatic analysis
11.06.2021: 15th meeting: Information exchange
18.06.2021: team Darmstadt Pubquiz, 16th meeting: Information exchange, 4th mentor meeting
19.06.2021: team Bielefeld HTML meeting
25.06.2021: 3rd Meeting with team Darmstadt: Information exchange and help, 17th meeting: Information exchange
28.06.2021: Safety instruction for the laboratory and meeting with Dr. Julia Götsch from Promega
02.07.2021: 18th meeting: Information exchange
03.07.2021: Russian meet-up
07.07.2021: 5th mentor meeting
09.07.2021: 19th meeting: Information exchange
16.07.2021: 20th meeting: Information exchange
22.07.2021: Meeting with Dr. Thomas Gassler to get more insight into Pichia pastoris expression systems
23.07.2021: 21st meeting: Information exchange
28.07.2021: 6th mentor meeting
06.08.2021: 22nd meeting: Information exchange
09.08.2021: Meeting with Geoff Williams for the Australian Platypus Conservancy (APC) talking about dairy industry and its impact on platypuses
11.08.2021: 7th mentor meeting
13.08.2021: 4th meeting with team Darmstadt: Information exchange and help, 23rd meeting: Information exchange
18.08.2021: Meeting with Dr. Knut Madden from Biogrammatics talking about food industry and the optimization of Pichia protocols
Meeting with Prof. Dr. Christopher Grefen about education and genetic engineering
20.08.2021: 24th meeting: Information exchange
25.08.2021: Meeting with Prof. Dr. Markus Piotrowski about education and genetic engineering in plants, 8th mentor meeting
26.08.2021: Meeting with Dr. Michael Krohn from the BrainAG to talk about Brazzein, the flavour and synthetic food industry
27.08.2021: 25th Meeting: Information exchange
31.08.2021: Meeting with Prof. Dr. Julia Bandow to talk about MLP and different screening methods of antimicrobial molecules
01-03.09.2021: Participation at the German Conference for Synthetic Biology
03.09.2021: 26th Meeting: Information exchange
07.09.2021: Meeting with Dr. Breanna Duffy from New Harvest about cellular agriculture, synthetic foods and entrepreneurship.
08.09.2021: 9th mentor meeting
10.09.2021: 5th meeting with team Darmstadt: Information exchange and help
12.09.2021: Travelling to the Lippe State Museum Detmold to inspect stuffed platypus
14.09.2021: Interview for the regional newspaper WAZ
17.09.2021: 27th meeting: Information exchange
22.09.2021: Teaching genetic engineering at school
The WDR (West German broadcasting) is filming inside our laboratory
10th mentor meeting
24.09.2021: 28th meeting: Information exchange
25.09.2021: Meeting iGEM Teams in Paris - iGEM Sorbonne and GO Paris-Saclay
29.09.2021: Taking part in the German Association for Synthetic Biology's "Biohackers Watch Party" and discussion about entrepreneurship with Dr. Britta Winterberg from Formo, Dr. Max Mundt Startup Origin.bio and Dr. Reyk Horland, Co-founder of the TissUse GmbH
30.09.2021: Live interview and TV feature at the WDR Lokalzeit Ruhr
03.10.2021: 29th meeting: Information exchange
06.10.2021: 11th mentor meeting
08.10.2021: 30th meeting: Information exchange
13.10.2021: 6th meeting with team Darmstadt: Information exchange and help
Taking part in the German Association for Synthetic Biology's "Biohackers Watch Party" and discussion with Dr. Dorothea Hämmerer, the journalist Janina Otto and science YouTuber Doktor Whatson
15.10.2021: 31st Meeting: Information exchange
18.10.2021: Meeting with Dr. Britta Winterberg about Formo, synthetic milk and entrepreneurship
12th mentor meeting
22.10.2021: 32nd meeting: Information exchange
27.10.2021: Meeting with Zoltan Toth-Czifra, founder of RealDealMilk
Laboratory
July to August:
Getting the lab space organised
Introduction to laboratory methods
Preparation of buffers and media
Preparation of competent cells E. coli DH5α
Preparation of genes and primers
August to September:
PCR and cloning of all constructs
Transformations
Check-PCRs and sequencing
Preparation of P. pastoris competent cells X33
September to October
Preparation of P. pastoris competent cells X33 repetition
Multiple transformations with the final construct into P. pastoris
