Team:RHS-Calgary/Results
Results
Experiment 1:
Testing Hydrogen Peroxide with the Colorimetric Assay Kit
Figure 1: Data demonstrates that Xylenol Orange from commercial colorimetric peroxide assay kit (in the Working Reagent solution) changes colour when exposed to Hydrogen Peroxide (H2O2).
The experiment demonstrated that the colorimetric assay kit does indeed change color when exposed to hydrogen peroxide. This is an essential part of our reaction as the color change is dependent entirely on the interaction between peroxide and the colorimetric assay kit. In this experiment, our hypothesis was proven true as the trials with 0.03% hydrogen peroxide changed color and those without hydrogen peroxide did not.
Experiment 2:
Testing the production of hydrogen peroxide through GOx
Figure 2: Results shown demonstrate that a colour change occurs in high concentrations of Glucose and GOx when diluted with a small volume of W.R., and a colour-changing reaction partially occurs when the volume of GOx is nearly that of W.R.
Experiment 3:
Testing the reaction between WR, GOx and Glucose, with multiple control trials
Figure 3: Data shows some successes and failures of negative controls when testing various dilutions of GOX and Glucose in 9 different trials, each containing 200 microlitres of W.R. All positive controls yielded successful results.