Team:RHS-Calgary/Parts
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Parts
| Species | Risk Group | Risk Group Source | Disease Risk to Humans? | Environmental Risk | Part Number | Function | Designer | Target Usage |
|---|---|---|---|---|---|---|---|---|
| Escherichia coli DH5-alpha | BSL-1 | PSDS | No | None | N/A | N/A | Commercial Seller | Chassis |
| Escherichia coli BL21 (DE3) | BSL-1 | PSDS | No | None | N/A | N/A | Commerical Seller | Chassis |
| B. subtilis | BSL-1 | PSDS | No | None | N/A | N/A | Commercial Seller | Chassis |
| Glucose Oxidase TEV cleavage site optimized for E. coli | N/A | N/A | No | None | BBa_K2238000 | To catalyze the oxidation of glucose | Commercial Seller | To produce hydrogen peroxide in order to cause a color change. |
parts
The parts included in our project are as follows:
Bacteria Species: Escheria coli BL21 (DE3), B. subtilis and DH5-alpha strain
Enzyme Species: Glucose Oxidase-Histag Aspergillus niger optimized for B. subtilis (BBa_K2934000) or Glucose Oxidase - A. niger (BBa_K2238000). (or BBa_K2238002)
In the future, we also aim to construct a plasmid and purify the protein in order to implement it into our project.