Parts Information of Nanjing-China
Several parts have been used for experiment in vitro, which were tailored elaborately by the whole team.
Here we present details of these parts for better use in the future,
including preparation methods, purification procedures, their functions and so on.
| Part number | Part name | Part type | Abstract | Matching Medal |
|---|---|---|---|---|
| BBa_K3731001 | mazE | Coding | The mazE codes a kind of antitoxin protein, which can help the bacteria grow longer and produce more polyP. | Silver |
| BBa_K3731002 | vgb | Coding | The vgb can produce HGB so that we can increase bacteria’s capacity of carrying oxygen, which is beneficial to the growth of bacteria. | Silver |
| BBa_K3731007 | vgb+mazE | Coding | This composite part is the combination of vgb and mazE gene, which are helpful for the growth of bacteria. | Silver |
| BBa_K3022002 | ppk1 in Citrobacter freundii ATCC 8090 | Coding | The polyphosphate kinase in Citrobacter freundii ATCC 8090 is responsible for its intracelluar inorganic polyphosphate (polyP) production via reversibly catalyzing the transfer of terminal phosphate from ATP to a growing polyP chain. We supplied background information for ppk1 in Citrobacter freundii ATCC 8090 and verified its functions in E.coli BL21. | Bronze |
| BBa_K1807009 | Polyphosphate kinase enzyme from Escherichia coli | Coding | The ppk1 in E.coli BL21 is responsible for its intracelluar inorganic polyphosphate (polyP) production via reversibly catalyzing the transfer of terminal phosphate from ATP to a growing polyP chain. | Bronze |
| BBa_K3731003 | ppk1+vgb+mazE | Coding |
This part is composed of ppk1, vgb and mazE. Adding vgb and mazE into the gene helps the growth of bacteria, thus improving the productivity of polyP. Part number for the existing part we improved: BBa_K3022002 |
Gold |