Team:Marburg/Notebook

Notebook

CW 49CW 50CW 51CW 52CW 53CW 01CW 02CW 03CW 04CW 05CW 06CW 07CW 08CW 09CW 10CW 11CW 12CW 13CW 14CW 15CW 16CW 17CW 18CW 19CW 20CW 21CW 22CW 23CW 24CW 25CW 26CW 27CW 28CW 29CW 30CW 31CW 32CW 33CW 34CW 35CW 36CW 37CW 38CW 39CW 40CW 41CW 42

CW 49: Mon Nov 30 2020 - Sun Dec 06 2020

  • Laboratory safety introduction
  • Introduction to the laboratory of Prof. Lars Voll and meeting the staff
  • IHP - Brainstorming and General Plans for Interviews
  • Member recruiting

CW 50: Mon Dec 07 2020 - Sun Dec 13 2020

  • Ordering of chemicals
  • Organizing the laboratory equipment
  • IHP - Interview PAW
  • Lab safety introduction

CW 51: Mon Dec 14 2020 - Sun Dec 20 2020

  • Planting the first tobacco plant in the phytochamber
  • First extensive meeting to plan the subgroups project

CW 52: Mon Dec 21 2020 - Sun Dec 27 2020

  • Testing the equipment
  • Planning the timeline
  • Elaboration of OpenTrons protocols

CW 53: Mon Dec 28 2020 - Sun Jan 03 2021

  • Testing the equipment
  • Planning the timeline
  • IHP - Interview Corteva
  • Cloning of first T7 test construct

CW 01: Mon Jan 04 2021 - Sun Jan 10 2021

  • Laboratory organization and planning of first experiments
  • Design of the dual luciferase lvl2 construct

CW 02: Mon Jan 11 2021 - Sun Jan 17 2021

  • First buffer and chemicals pereparation
  • First cell free measurement test

CW 03: Mon Jan 18 2021 - Sun Jan 24 2021

  • First tobacco chloroplast isolation with sucrose gradient
  • Purification of genomic DNA from Oak

CW 04: Mon Jan 25 2021 - Sun Jan 31 2021

  • First tobacco chloroplast extract
  • IHP - Interview DBV
  • Creation of first parts from Oak

CW 05: Mon Feb 01 2021 - Sun Feb 07 2021

  • First spinach chloroplast isolation with sucrose gradient
  • Started DNA Extraction of various plants

CW 06: Mon Feb 08 2021 - Sun Feb 14 2021

  • First spinach chloroplast extract
  • First functioning cell free extract measurement from Tobacco

CW 07: Mon Feb 15 2021 - Sun Feb 21 2021

  • Testing tobacco choroplast isolation with recycled conical tubes
  • Collaboration - Contact with iGEM Madrid_UCM
  • IHP - Interview KWS

CW 08: Mon Feb 22 2021 - Sun Feb 28 2021

  • Testing tobacco choroplast isolation with half frozen medium and sucrose gradients
  • IHP - Exchange with Lauren Clark
  • Start cloning of lvl2 Dropout constructs

CW 09: Mon Mar 01 2021 - Sun Mar 07 2021

  • Testing lysis methods: needle chloropast disruption vs sonication
  • Collaboration - Shipping of genetic parts to Madrid

CW 10: Mon Mar 08 2021 - Sun Mar 14 2021

  • First tobacco chloroplast isolation with Percoll gradient
  • Purification of genomic DNA from Tobacco

CW 11: Mon Mar 15 2021 - Sun Mar 21 2021

  • Testing sucrose vs Percoll density gradients during spinach chloroplast isolation
  • HP - Interview ZKBS
  • First functioning cell free extract measurement from Spinach

CW 12: Mon Mar 22 2021 - Sun Mar 28 2021

  • Testing chloroplast isolation using buffers without and with B-mercaptoethanol
  • Start cloning of in vivo constructs

CW 13: Mon Mar 29 2021 - Sun Apr 04 2021

  • First wheat chloroplast isolation with percoll gradient

CW 14: Mon Apr 05 2021 - Sun Apr 11 2021

  • First wheat chloroplast extract
  • IHP/HP - Integration of several ideas of IHP Interviews into HP Project design
  • Purification of genomic DNA from Spinach and Wheat

CW 15: Mon Apr 12 2021 - Sun Apr 18 2021

  • First rice chloroplast isolation with Percoll gradient
  • Partnership - First Meeting Bielefeld/Marburg
  • Reiteration of Firefly cassette for in vivo construct

CW 16: Mon Apr 19 2021 - Sun Apr 25 2021

  • First rice chloroplast extract
  • IHP - Implementation of ideas from Lauren Clark
  • Order of the first primer plate for Spinach parts

CW 17: Mon Apr 26 2021 - Sun May 02 2021

  • Measurement of cell-free extracts and troubleshooting

CW 18: Mon May 03 2021 - Sun May 09 2021

  • First oak chloroplast isolation with Percoll gradient
  • Partnership - Brainstorming of Ideas for Partnership
  • HP - Ask EFSA
  • First test of Firefly luciferase expression

CW 19: Mon May 10 2021 - Sun May 16 2021

  • First oak chloroplast extract
  • Collaboration - German Meetup held by Bonn & Kaiserslautern
  • HP - Dies Academicus
  • Order of the second and third primer plate for Wheat and Rice parts

CW 20: Mon May 17 2021 - Sun May 23 2021

  • Tobacco seed sterilization and plating
  • Collaboration - iGEM Monument challenge
  • First test of cell-free extract from Oak

CW 21: Mon May 24 2021 - Sun May 30 2021

  • Tobacco seeding transfer to sterile culture
  • Partnership - Preparation of Slack-Workspace and other stuff
  • First functioning cell-free extract measurement from Wheat

CW 22: Mon May 31 2021 - Sun Jun 06 2021

  • First barley and tomato chloroplast isolation with sucrose gradient
  • Collaboration - Postcard iGEM Düsseldorf
  • Successful endogenous transcription using our best basic part

CW 23: Mon Jun 07 2021 - Sun Jun 13 2021

  • First barley and tomato chloroplast extract
  • Optimization of coffee preparation
  • Purification of genomic DNA from Rice

CW 24: Mon Jun 14 2021 - Sun Jun 20 2021

  • First maize chloroplast isolation with sucrose gradient
  • HP - Lord Mayor
  • Finished cloning of in vivo constructs

CW 25: Mon Jun 21 2021 - Sun Jun 27 2021

  • First maize chloroplast extract
  • Partnership - First Meet-Up
  • HP - KiJuPa
  • Sequencing of Spinach parts

CW 26: Mon Jun 28 2021 - Sun Jul 04 2021

  • Measurement of cell-free extracts of tobacco
  • Collaboration - Russian Meet-Up
  • HP - Minister
  • First test of cell-free extract from Rice

CW 27: Mon Jul 05 2021 - Sun Jul 11 2021

  • Partnerhip Paris Bettencourt - Start of weekly meetings of Remote Lab project
  • Sequencing of Wheat parts
  • Measurement of cell-free extracts of tobacco, rice, maize, soy, tomato and barley

CW 28: Mon Jul 12 2021 - Sun Jul 18 2021

  • Measurement troubleshooting of cell-free extracts
  • Collaboration - iGEM Macau cookbook
  • Optimization of endogenous transcription using differenct composition of translational buffer

CW 29: Mon Jul 19 2021 - Sun Jul 25 2021

  • Testing lysis methods: amount of pushes through a needle
  • Partnership - Second Meet-Up
  • Finished cloning of all lvl2 Dropout constructs

CW 30: Mon Jul 26 2021 - Sun Aug 01 2021

  • Measurement of cell-free extracts with varying magnesium and potassium salt concentrations
  • Design for the remaining DNA Synthesis

CW 31: Mon Aug 02 2021 - Sun Aug 08 2021

  • Plate preparation for biolistic transformation of tobacco
  • Sequencing of Rice parts

CW 32: Mon Aug 09 2021 - Sun Aug 15 2021

  • Biolistic tobacco transformation
  • Recloning of universal T7 test construct

CW 33: Mon Aug 16 2021 - Sun Aug 22 2021

  • Testing different dialysis casettes for chloroplast extracts
  • Partnership - Third Meet-Up
  • IHP - Interview Northwestern University
  • First test of lvl2 dual luciferase construct

CW 34: Mon Aug 23 2021 - Sun Aug 29 2021

  • Testing different dark incubation times for tobacco chloroplast isolation
  • Collaboration - iGEM Thrace
  • Test of Luminescence normalization using purified NanoLuc luciferase

CW 35: Mon Aug 30 2021 - Sun Sep 05 2021

  • Measurement of cell-free extracts
  • Partnership - Working on Booklet
  • Finish the production of natural lvl0 parts

CW 36: Mon Sep 06 2021 - Sun Sep 12 2021

  • Production of large quantities of tobacco chloroplast extract
  • IHP - Lab implementation of feedback from the Interview with Northwestern; HP - first course with BioBits
  • Optimization of the Luminol normalization protocol

CW 37: Mon Sep 13 2021 - Sun Sep 19 2021

  • Production of large quantities of spinach chloroplast extract
  • Partnership - Forth Meet-Up
  • HP - Farmer Interview
  • Successful test of synthetic 5'UTRs including PPR binding sites

CW 38: Mon Sep 20 2021 - Sun Sep 26 2021

  • Testing lyophilisation of chloroplast extracts
  • Characterization of the first 21 part making use of our dual luciferase construct

CW 39: Mon Sep 27 2021 - Sun Oct 03 2021

  • Measurement of cell-free extracts
  • HP - Second course with BioBits
  • Characterization of the wheat parts making use of our dual luciferase construct

CW 40: Mon Oct 04 2021 - Sun Oct 10 2021

  • Last tobacco chloroplast isolations with Percoll gradient
  • Partnership - Bielefeld Team visit Marburg
  • Successful test of intercistronic expression element in cell-free extract from Tobacco

CW 41: Mon Oct 11 2021 - Sun Oct 17 2021

  • Antibiotic resistance test for tobacco and wheat
  • Partnership Paris Bettencourt - Last meeting of Remote Lab project
  • IHP - Test Proof of concept "High Throughput Testing of different antibiotics""
  • Preparation of cryostocks

CW 42: Mon Oct 18 2021 - Sun Oct 24 2021

  • PCR analysis to confirm transgene integration in tobacco
  • Wrapping up labwork