Contribution
Parts
Par101
Partition (par) locus from the pSC101 plasmid is required for stable maintenance of the plasmid in a bacterial cell population.
It is a binding site for DNA gyrase. The stabilization of plasmid inheritance is mediated by the ability of par to generate negative supercoils in plasmid DNA. This element is essential for correct segregation, in lack of par a half of cells lose the plasmid.
BLM RecQ like helicase
ATP-dependent DNA helicase that unwinds single- and double-stranded DNA in a 3'-5' direction. It is involved in several pathways contributing to the maintenance of genome stability.
- Participates in DNA replication and repair
- Involved in 5'-end resection of DNA during double-strand break (DSB) repair
- Negatively regulates sister chromatid exchange (SCE)
- Stimulates DNA 4-way junction branch migration and DNA Holliday junction dissolution
- Binds single-stranded DNA (ssDNA), forked duplex DNA, and DNA Holliday junction
Source: UniProt
Anti PDCD11 siRNA
PDCD11 is a NF-kappa-B binding protein that colocalizes with U3 RNA in the nucleolus and is required for rRNA maturation and generation of 18S rRNA.
Anti PDCD11 siRNA targets PDCD11 gene and regulates the expression of the gene through the mechanism of RNA- interference. The siRNA interferes with PDCD11 mRNA as part of the multiprotein RISC complex and provides mRNA degradation, preventing translation. It has potential applications as a radioprotective molecule in radiotherapy, space flights, and other cases of exposure to ionizing radiation on humans.
Anti Tbx2 siRNA
Tbx2 is involved in several developmental processes such as coordinating cell fate, patterning and morphogenesis of a wide range of tissues and organs including limbs, kidneys, lungs, mammary glands, heart, and craniofacial structures.
It has been seen that, Tbx2 is overexpressed in several cancers including melanoma, small cell lung carcinoma, breast, pancreatic, liver, and could suppress senescence, a cellular process, which serves as a barrier to cancer development.
Anti Tbx2 siRNA targets Tbx2 gene and regulates the expression of gene through mechanism of RNA- interferece. The siRNA interferes with Tbx2 mRNA as part of multiprotein RISC complex and provides mRNA degradation, preventing translation. It has potential applications as radioprotective molecule in radiotherapy, space flights and other cases of exposure to ionizing radiation on humans and gene therapy of cancers.
Wnt10a
Wnt proteins constitute a large family of secreted glycoproteins that control diverse aspects of embryonic development and adult homeostasis
They have influence in the balance between proliferation and differentiation in many cell types, they are implicated in the biological processes like bone formation, immune regulation, cancer, and stem cell renewal.
Many studies have linked activation of Wnt/β-catenin signaling pathway to radioprotection, especially of salivary gland, oral mucosa, and gastrointestinal epithelium through inhibition of apoptosis and preservation of normal tissue functions. Wnt10a has been reported as one of the most overexpressed proteins in inhabitants of high background-radiation areas.
http://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2021&group=MEPhIWe improved our parts BBa_K4049010 and BBa_K4049000 through adding arabinose inducible promoter pBAD in order to get time specific expression of Wnt and BLM proteins to minimize side effects in the case when we use E.Coli, since they are able to integrate into biofilm.
Lactobacteria do not integrate into biofilm, and will be secreted in a few hours, so for them we do not need an inducible promotor.
We made this for our Improvement of an Existing Part, but,unfortunately, we have problems with the Regestry. So we would like to show you it here.
