Team:Lund/Attributions

iGEM Lund 2021

iGEM Lund 2021

Attributions

We are very thankful for all the people that helped us in our project. Without them project Cure-li would not have happened, thank you!

Dr. Johan Svensson Bonde

Researcher at the Department of Pure and Applied Biochemistry

He was our PI and without him iGEM Lund 2021 would not have been able to complete the project. He was the only instructor this year and supported us throughout the project. 

Dr. Cedric Dicko

Associate Professor at the Department of Pure and Applied Biochemistry

Helped us tremendously with curli measurements, including  the protocols and analysis of the results.

Dr. Zack Abbot

PhD in Microbiology and Immunology, Entrepreneur, CEO and founder of ZBiotics

Discussed the marketing aspects of the potential product and some lab discussions about colonization and L. reuteri.

Dr. Srikkanth Balasubramanian

PhD in Microbiology, Researcher at TU Delft

Insight on MC4100 E.coli strain and how to obtain curli and aid in the search of MC4100 -CsgA.

Dr. Anne Farewell

PhD and Senior Lecturer in the Department of Chemistry and Molecular Biology at the University of Gothenburg

Provided our team with the curli-producing E. coli strain MC4100.

Johan Furuhjelm

Student at the Faculty of Engineering at Lund University

Edited the iGETHICS and presentation video. Created the iGETHICS intro.

Erik Hartman

Founder of SynthEthics

Provided helpful feedback to our article published on the SynthEthics platform.

Dr. Michela Lizier

Lab technician, IRCCS Humanitas Research Hospital

Gifted us the plasmid pTRKH3-ermGFP (Addgene plasmid # 27169 ; http://n2t.net/addgene:27169 ; RRID:Addgene_27169)

Fredrik Lund

PhD student at division of Applied Microbiology at The Faculty of Engineering at Lund University, LTH

Lent a microscope and a Neubauer chamber to the lab to carry on a few experiments.

Dr. Georg Meisl

PhD in Biophysical Chemistry, University of Cambridge

Reviewed our approach to modelling the inhibitors. Gave us critical approaches to modelling chaperones. 

Dr. Anne Meyer

PhD in Biological Sciences, Associate Professor at University of Rochester

Shared insight on MC4100 E.coli strain and how to obtain curli and aid in the search of MC4100 -CsgA.

Martin Palm

PhD student at the Department of Chemistry and Molecular Biology at the University of Gothenburg

Provided our team with the curli-producing E. coli strain MC4100.

Jordi Riera i Alsina

Chief Business Development Officer at AB-BIOTICS

Explained their products related with the gut-brain axis; how they are made, acceptance in the market, dose etc.

Dr. Stefan Roos

Senior Microbiologist BioGaia AB; Professor, adjunct, Food Biotechnology, Swedish University of Agricultural Sciences

Provided helpful comments concerning the pTKRH3 plasmid.

Manuel Alejandro Ruiz Mejia

Research Student at the Faculty of Engineering at Lund University

Provided our team with the L. reuteri DSM 20016 strain and lent us some useful material.

Lovisa Wallman

M.Sc. in Biotechnology Engineering, Molecular Biotechnology

Provided our team with digested inserts and pET11a plasmid to use as controls.

Dr. Puwei Yuan

Project Leader at NeoX

Shared in depth insight in how to cultivate the MC4100 E.coli strain and obtain curli.

Sponsors

We are very grateful for all of our sponsors and thank them immensely for what they have done for us.

Faculty of Engineering at Lund university 

Educational institution 

They provided us with funding. Which made sure we were able to realise our project. 

LMK foundation

Foundation focused on funding projects with a interdisciplinary scientific research.

They provided us with funding. Which made sure we were able to realise our project. 

IDT (Integrated DNA Technologies)

Company that creates custom DNA parts

Provided us with free DNA base pairs that allowed us to execute our lab. 

Twist Bioscience 

Company that creates custom DNA parts

Provided us with free DNA base pairs that allowed us to execute our lab.

Benchling

Online software for designing, recording and analyzing experiments.

Provided us with the opportunity to document our lab work in the online notebook as well as a tool for analyzing our sequences.