Utilized and characterized existing parts

Part name	Part number	Contribution
NT2RepCT	BBa_K3264000	Used as a spider-silk fibrion control. We characterized its expression and solubility in E. coli BL21 DE3. We also tested the physical properties of BCM mixed with NT2RepCT.
ptac-RiboJ	BBa_K3552015	As an IPTG-inducible promoter, we used it to express our dye-producing enzymes such as Fre-SttH and TnaA-linker-FMO.
sfGFP	BBa_I746916	We constructed a fusion protein with sfGFP to test the viability of our Ma secretion system in S. cerevisiae.
TALEsp2-tnaA-RBS-fmo	BBa_I746916	Originally a complete indigo production pathway, we tested the new substrates Br-Trp and Cl-Trp and measured its tyrian purple titer.
pTEF1	BBa_K319003	As an yeast promoter, we used it in expressing acetyl-CoA producing enzymes

New basic parts

Part name	Part number	Sequence correct?	Does it function?	Group favorite
CBM3	BBa_K4011000	Yes	Yes	Yes
CBM2	BBa_K4011001	Yes	Does not work fusion spidroin proteins
Ma-Mut	BBa_K4011002	Yes	Yes
fre-sttH	BBa_K4011003	Yes	Yes	Yes
tnaA-FL-fmo	BBa_K4011004	Yes	Yes	Yes
tnaA-RL-fmo	BBa_K4011005	Yes	Yes	Yes
AeAT9	BBa_K4011006	Yes
SaACS2	BBa_K4011007	Yes

Part name	Part number	Composite of:	Sequence correct?	Does it function?	Group favorite
ptac-RiboJ-tnaA-RL-fmo-B0015	BBa_K4011013	BBa_K3552015+BBa_K4011005+BBa_B0015	Yes	Yes
ptac-RiboJ-tnaA-FL-fmo-B0015	BBa_K4011014	BBa_K3552015+BBa_K4011004+BBa_B0015	Yes	Yes	Yes
ptac-RiboJ-tnaA-RBS-fmo-B0015	BBa_K4011015	BBa_K3552015+BBa_K3264025+BBa_B0034+BBa_K1131000+BBa_B0015	Yes	Yes	Yes
pTEF1-SaACS2-tADH	BBa_K4011016	BBa_K319003+BBa_K4011007+BBa_K4012022	Yes	No functional data prior wiki freeze
pRPL8B-AeAT9-tSSA1	BBa_K4011017	BBa_K4011020+BBa_K4011007+BBa_K4012020	Yes	No functional data prior wiki freeze
pTEF1-SaACS2-tADH1-pRPL8B-AeAT9-tSSA1	BBa_K4011018	BBa_K4011016+BBa_K4011017	Partially	No functional data prior wiki freeze
pTALEsp2-tnaA-FL-fmo	BBa_K4011019	BBa_K2754019+BBa_K4011004	Partially	No functional data prior wiki freeze