Team:Ecuador/Parts

<!DOCTYPE html> Project IGem Ecuador

Parts

Parts Collection

Parts

For Modules 1 and 2 (see Project modules) we designed sequences and cassettes for dsRNA production and the Quorum sensing-Lysis system, respectively.

All our basic parts are compatible with the BioBricks grammar, as they do not contain illegal sites for RFC10.

Basic Parts Table

Name Type Description Designer Length
BBa_K3893000 RBS ShineDalgarno for E coli Camila Gallegos 9
BBa_K3893001 RNA dsRNA designed for the target gene Velvet Carolina Panchana 472
BBa_K3893002 RNA dsRNA designed for the target gene ERG11 Carolina Panchana 397
BBa_K3893003 RNA dsRNA designed for the target gene SGE1 Carolina Panchana 483
BBa_K3893004 RNA dsRNA designed for the target gene SIX1 Carolina Panchana 486
BBa_K3893005 CDS Lysozyme C (Lysis protein) Camila Gallegos 441
BBa_K3893006 Terminator rpoC Transcriptional terminator Stefania Montesinos 83
BBa_K3893007 Terminator Reverse of BBa_M36010 / rnpB_T1 Tannya Sandoval 82
BBa_K3893019 RBS Reverse of BBa_B0034 / RBS2 o RBS Tannya Sandoval 12
BBa_K3893020 Promoter Reverse of BBa_I712074 / Promoter T7 Tannya Sandoval 46
BBa_K3893021 RBS Reverse of BBa_K3893000 / ShineDalgarno for E. coli Stefania Montesinos 9
BBa_K3893022 RBS Reverse of BBa_B0032 / RBS3 Stefania Montesinos 13

Composite Parts Table

Name Type Description Designer Length
BBa_K3893017 Composite GFP translational unit Stefania Montesinos 797
BBa_K3893018 Composite Reverse translational unit for GFP Stefania Montesinos 797
BBa_K3893008 Composite Up part of cassette for dsRNA constitutive production 1 Tannya Sandoval 969
BBa_K3893009 Composite Down part of cassette for dsRNA constitutive production 1 Tannya Sandoval 966
BBa_K3893010 Composite Up part of cassette for dsRNA constitutive production 2 Sharon Ortiz 983
BBa_K3893011 Composite Down part of cassette for dsRNA constitutive production 2 Sharon Ortiz 981
BBa_K3893012 Composite Up part of cassette for dsRNA constitutive production 3 Tannya Sandoval 967
BBa_K3893013 Composite Down part of cassette for dsRNA constitutive production 3 Sharon Ortiz 968
BBa_K3893014 Composite Up part of cassette for dsRNA constitutive production 4 Camila Gallegos 984
BBa_K3893015 Composite Down part of cassette for dsRNA constitutive production 4 Camila Gallegos 982
BBa_K3893016 Plasmid_Backbone BioBricks compatible L4440 plasmid Camila Gallegos 2550
BBa_K3893026 Composite luxI constitutive expression Stefania Montesinos 787
BBa_K3893027 Composite AHL induced expression of Lysis protein Stefania Montesinos 506
BBa_K3893028 Composite AHL induced expression of GFP (including luxR constitutive expression) Stefania Montesinos 1903
BBa_K3893029 Composite AHL induced expression of phiX174 lysis protein TU and luxI constitutive expression TU Stefania Montesinos 1297
BBa_K3893026 Composite Population control device (QS-based lysis protein oscilator) Stefania Montesinos 3200