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Parts
For Modules 1 and 2 (see Project modules) we designed sequences and cassettes for dsRNA production and the Quorum sensing-Lysis system, respectively.
All our basic parts are compatible with the BioBricks grammar, as they do not contain illegal sites for RFC10.
Basic Parts Table
Name | Type | Description | Designer | Length |
---|---|---|---|---|
BBa_K3893000 | RBS | ShineDalgarno for E coli | Camila Gallegos | 9 |
BBa_K3893001 | RNA | dsRNA designed for the target gene Velvet | Carolina Panchana | 472 |
BBa_K3893002 | RNA | dsRNA designed for the target gene ERG11 | Carolina Panchana | 397 |
BBa_K3893003 | RNA | dsRNA designed for the target gene SGE1 | Carolina Panchana | 483 |
BBa_K3893004 | RNA | dsRNA designed for the target gene SIX1 | Carolina Panchana | 486 |
BBa_K3893005 | CDS | Lysozyme C (Lysis protein) | Camila Gallegos | 441 |
BBa_K3893006 | Terminator | rpoC Transcriptional terminator | Stefania Montesinos | 83 |
BBa_K3893007 | Terminator | Reverse of BBa_M36010 / rnpB_T1 | Tannya Sandoval | 82 |
BBa_K3893019 | RBS | Reverse of BBa_B0034 / RBS2 o RBS | Tannya Sandoval | 12 |
BBa_K3893020 | Promoter | Reverse of BBa_I712074 / Promoter T7 | Tannya Sandoval | 46 |
BBa_K3893021 | RBS | Reverse of BBa_K3893000 / ShineDalgarno for E. coli | Stefania Montesinos | 9 |
BBa_K3893022 | RBS | Reverse of BBa_B0032 / RBS3 | Stefania Montesinos | 13 |
Composite Parts Table
Name | Type | Description | Designer | Length |
---|---|---|---|---|
BBa_K3893017 | Composite | GFP translational unit | Stefania Montesinos | 797 |
BBa_K3893018 | Composite | Reverse translational unit for GFP | Stefania Montesinos | 797 |
BBa_K3893008 | Composite | Up part of cassette for dsRNA constitutive production 1 | Tannya Sandoval | 969 |
BBa_K3893009 | Composite | Down part of cassette for dsRNA constitutive production 1 | Tannya Sandoval | 966 |
BBa_K3893010 | Composite | Up part of cassette for dsRNA constitutive production 2 | Sharon Ortiz | 983 |
BBa_K3893011 | Composite | Down part of cassette for dsRNA constitutive production 2 | Sharon Ortiz | 981 |
BBa_K3893012 | Composite | Up part of cassette for dsRNA constitutive production 3 | Tannya Sandoval | 967 |
BBa_K3893013 | Composite | Down part of cassette for dsRNA constitutive production 3 | Sharon Ortiz | 968 |
BBa_K3893014 | Composite | Up part of cassette for dsRNA constitutive production 4 | Camila Gallegos | 984 |
BBa_K3893015 | Composite | Down part of cassette for dsRNA constitutive production 4 | Camila Gallegos | 982 |
BBa_K3893016 | Plasmid_Backbone | BioBricks compatible L4440 plasmid | Camila Gallegos | 2550 |
BBa_K3893026 | Composite | luxI constitutive expression | Stefania Montesinos | 787 |
BBa_K3893027 | Composite | AHL induced expression of Lysis protein | Stefania Montesinos | 506 |
BBa_K3893028 | Composite | AHL induced expression of GFP (including luxR constitutive expression) | Stefania Montesinos | 1903 |
BBa_K3893029 | Composite | AHL induced expression of phiX174 lysis protein TU and luxI constitutive expression TU | Stefania Montesinos | 1297 |
BBa_K3893026 | Composite | Population control device (QS-based lysis protein oscilator) | Stefania Montesinos | 3200 |