Team:Ecuador/Attributions
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Team
Attributions
Collaborations
General Support
We sincerely thank our principal investigators, PhD Francisco Flores and PhD Yadira Boada, for supervising the progress of our project and helping us to deal with the difficulties we encountered.
Wet Lab
Team Leaders
Fernanda Arias and Jean Herdoiza organized and delegated the activities.
Team Players
The group has 6 members (Carolina Panchana, Vanessa Gaona, Xavier Montalvo, Lorena Troya, Tannya Sandoval, Camila Armas)Identification of genes for RNA silencing in Fusarium oxysporum (FOC) f. sp. cubense race 1 was carried out by Camila Armas and Carolina Panchana. The first stage for obtaining dsRNA was carried out by Jean Herdoiza. Vanessa Gaona, under the instruction of Estefany Paredes, performed the assemblies corresponding to the plasmid optimization for dsRNA production. Carolina Panchana and Fernanda Arias worked on part improvement of lysis protein.Xavier Montalvo and Lorena Troya obtained conidia of FOC race 1 for inhibition assays. Tannya Sandoval and Camila Gallegos supported the standardization of techniques.
Lab Support
We thank our principal investigator,PhD Francisco Flores, for giving us the confidence to work in his private laboratory at iDGen when the lab at our university was closed due to the COVID pandemic.
Eduardo Moncayo and Francisco Mosquera (Biotechnology engineers and our advisors) guided our lab work with their extensive experience.
Francisco Garrido (Biotechnology engineer and iDGen lab technician) assisted us with technical suggestions for our project and gave us support in the lab.
Collaborators
Mateo Molina, Janyna Calderón, Josué Calderón, Paúl Márquez, Valeria Cevallos, Jatari Erazo, Belén Alulema, Camila Granda, Alisson Changoluisa, Cristina Pilamunga interns in our lab, collaborated with us performing basic lab techniques including culture media preparation, plasmid extraction, reagent preparation and others.
Otto Heringer (Chemist with specialization in biochemistry and molecular biology) and Jennifer Tapia (Biotechnology engineer and OSU master's degree candidate) kindly gave us the strain E.coli HT115 and L4440 plasmid for our assays.
PhD Yadira Boada donated some plasmids from the Valencia_UPV iGEM team 2018 and collaborated with the Module 2 Delivery and Release assays.
Fusarium oxysporum f. sp. cubense race 1 strain was provided by Freddy Magdama PhD from ESPOL university, Ecuador.
DryLab (Modeling)
Team Leaders
Stefania Montesinos organized and delegated the activities, supervised by Natalia Torres.
Team Players
The group consists of 4 members (Camila Gallegos, Carolina Panchana, Tannya Sandoval, Sharon Ortiz)
The homology and specificity analysis of the proposed genes, as well as the design of their dsRNAs, was carried out by Carolina Panchana, under the guidance of Estefany Paredes. The design of the cassettes, in silico assemblies and the design of primers for the entire project; it was made by Stefania Montesinos, Camila Gallegos, Tannya Sandoval and Sharon Ortiz ; with the guidance and instruction of Natalia Torres, Juan Carlos Luzuriaga and Camila Velandia. The modeling was in charge of Stefania Montesinos with the constant support of Natalia Torres and the help of Juan Carlos Luzuriaga and Camila Velandia.
Support mentors
A special thanks to our second PI, PhD Yadira Boada, who guided and supported us in the design of cassettes and modeling of our project.
Collaborators
Esteban Mena, Fernando Vivanco, Jessith Salinas, Karolay Hernández, Raquel Andrango, Evelyn Pulles interns in our drylab, collaborated with us in research sequences, assembly RFC-10 and primer design.
Hardware
Team Leader
Luis Moyano organized and delegated the activities.
Team Players
Special recognition to all participants in the Hardware team: Karla Gaona, Jonathan Gavilanes, Sharon Ortiz y Paul Ramirez for the effort and dedication in fulfilling the activities required throughout the device construction.
Support mentors
A special thanks to our mentors who were a fundamental part of the design and construction of the teams. Fernando (from Valencia UPV 2016 iGEM team) advised us on the design of the fluorometer explaining the fundamentals of its operation and previous experiences. Yadira Boada helped us with the design and selection of the filters as well as the communication with different experts such as Fernando. Eduardo Tayupanta advises us on IOT communication which allows us to connect the devices to the internet.
Yadira Boada Researcher & Associate Professor, Instituto de Automática e Informática Industrial, Spain.
Nobel Santos Electrical engineer specialized in control. Currently, he studying for an industrial automation master in the UPV. He entered the world of biotechnology by chance, however, he is here to stay.
Eduardo Tayupanta Electronic Engineer in Automation and Control in 2018 and Master's Degree in Artificial Intelligence in 2020. He participated in companies such as Ecuagraphic, ENS Electronics, ESPE Innovativa E.P. as a software developer and with the company Mobility in the implementation of Control Systems for the Floricola FlowerVillage. He works as an IoT Technology Administrator at AXXIS Medical Center and teaches programming classes at ESPE Innovativa E.P.
Wiki (Graphic Design)
Team leaders
Sharon Ortiz led the organization of the activities.
Team Players
The group has 1 member, Stefania Montesinos a, she worked on the wiki alongside Sharon, they designed the wiki website and organized the information collected from each group: Wetlab, Drylab, and others, so that it is clearly projected on the wiki. They received recommendations from Estefany Paredes and Daniela Sempertegui The entire team did a great creative job.
Support Mentors
Yadira Boada provided feedback about wiki information Ricardo Alejandro, who helped us raise money for our team. Luis Torres, he helped us design some icons for our project. Leandro Alvarez, he helped us by instructing us on the code we need to know from the wiki.
Collaborators
Interns Bryan Cevallos, Fátima Guanoluisa, Milenka Vera y Josselyn Chacón helped us with the development of the wiki. Fátima designed the icons for representing our subgroups.
Human Practices
Team Leaders
Xavier Montalvo and Carolina Panchana organized and delegated the activities.
Team Players
The group consists of 2 members Camila Armas and Lorena Troya, and 5 interns María Teresa Ochoa, Kevin Masabanda, Carolina Guamán, Jatari Erazo, and Leidy Yazán.
Camila Armas and Xavier Montalvo were in charge of planning the consultancies with the scientific experts. Carolina Panchana and Xavier Montalvo were in charge of bringing together the groups that make up the banana value chain. Camila Armas, Lorena Troya, Xavier Montalvo and Carolina Panchana were in charge of bringing together these groups: small banana producers, government entities, experts in musaceae diseases, suppliers of plant material, and fruit exporters, respectively, with the supervision of Juan Carlos Luzuriaga.
Xavier Montalvo, Carolina Panchana, and the interns: María Teresa Ochoa, Kevin Masabanda, Carolina Guamán, Jatari Erazo, and Leidy Yazán were in charge of finding the information and elaborating the deliverables for the “Global Shipping Policy” event organized by After iGEM 2021.
Collaborators
Otto Heringer, and Salomé Suárez provided information on dsRNA design. Alexis Vela from the Ministry of the Environment helped us and collaborated with us to ensure that the project complies with environmental regulations, in addition to providing constant advice on the process that must be followed to patent biotechnological products. Antonio Bustamante helped us by proposing a dsRNA administration system.
Education
Team Leader
Camila Gallegos organized and delegated the activities, under the instruction of Natalia Torres.
Team Players
The group consisted of five iGEM members (Vanessa Gaona, Tannya Sandoval, Paúl Ramírez, Luis Moyano, Jonathan Gavilanes) and 4 interns (Natalia Romero, María Mendoza, Naomi Gangotena, César Chalco) iGEM members contributed to the development of talks and conferences on SynBio, iGEM, and Agrobactory 593, given in schools, universities, and institutes; they developed home experiments and organized the scientific gymkhana in the indigenous community of the Ecuadorian Amazon. Luis Moyano, Jonathan Gavilanes and Paúl Ramírez designed and built the transilluminator, a tool that helped us to democratize synthetic biology in low-income indigenous communities in Ecuador. Our interns organized the talk in the indigenous community of Ibarra, looking for the best tools to communicate about SynBio to adolescents. They also created a story to communicate our project through illustrations and simple words, aimed at young children.
Entrepreneurship
Team Leader
Vanessa Gaona organized and delegated the activities, supervised by Camila Velandia.
Team Players
Special recognition to all participants in the Agrobactory 593 entrepreneurship: Luis Moyano, Paul Ramírez, for the effort and dedication in fulfilling the activities required throughout the process of entrepreneurship.
Support mentors
Special thanks to our mentors who helped us throughout the process. Sofía González was our advisor throughout the entire pre-incubation process and helped us with Value Proposition Canvas and Buyer persona, Andrés Cueva helped us with Pitch Review and Business Model Review, Jhony Villacís mentored us with Business models for technological devices, Alexis Vela helped us with Risk assessment analysis for biotechnology products and Bioentrepreneurship and Cristina Arteaga provided us with her financial knowledge to carry out the activities during the pre-incubation process. Juan Carlos Luzuriaga, Camila Velandia, Daniela Sempertegui supervised all the activities.
Sofía González, Co-founder of Latinnova. Analyst of entrepreneurship and innovation projects at the University of the Armed Forces - ESPE. Mentor and trainer in entrepreneurship, innovation, and female empowerment in the AWE program - Academy for Women Entrepreneurs of the US Embassy and the Quito Chamber of Commerce.
Andrés Cueva, Master of Information Systems with a focus on emerging technologies and innovation from the University of Melbourne-Australia, is a specialist in innovation and entrepreneurship at UFA-ESPE, teaches master's degrees, co-founder of Re-Make, member of FabLab Ecuador, RELIIE and mentor of entrepreneurship and innovation in different institutions and programs nationwide.
Jhony Villacis, Electronic engineer with a specialization in Project Management at the Polytechnic University of Madrid, I have a diploma in Product Management and a diploma in StartUps Creation as well as my experience in Product Manager, Business Models, and Agilism.
Alexis Vela Arias, Biotechnology Engineer and Biosafety specialist from the Ministry of Environment, works as an environmental permit documentation and negotiation assistant with a diploma in Biotechnology, Bioeconomy, Industry, and Business.
Cristina Arteaga, Financial Engineer, CPA Auditor, Three years of experience between financial area and external audit developing analytical skills to identify financial risks, data processing analysis & projections.
Organization / Communication
Team Leaders
María Fernanda Arias and Jean Herdoiza, as the team leaders, organized and coordinated all the team throughout the whole project.
Social Media was managed by Jean Herdoiza. Video Production was developed by Carlos Andres Parra from “Bioscience By Carlos Parra”
