Difference between revisions of "Team:ECNUAS/Safety"

In the 12-day training, safety instructions are indispensable for ones who frequently do experiments in the laboratory. On the first day of entering the laboratory, our supervisor gave a lesson about laboratory safety. First, laboratory clothes must be worn neatly, which helps protect us from being harmed by hazardous substances during experiments. Second, long hair must be tied or arranged, so that the solitary hair will not influence experiment progress or shield eyesight. Third, food and beverage are prohibited from entering the laboratory which prevents the experimental area from been polluted by them. After finishing the experiment, hands must be washed before leaving. Fourth, laboratory equipment, such as microwaves and refrigerators, are not allowed to preserve or put an impact on food. Fifth, do not touch electrical equipment with wet hands. Sixth, keep the surroundings nice and clean, and strictly differentiate the experimental and clean areas. Seventh, prepare equipment needed for later experiments and review the process. Eighth, all experimental wastes should be sorted and carefully treated before abandoning. Due to the epidemic, the school started to take our temperature every morning to make sure that we have no evidence of having a fever.

The microorganisms and experimental equipment we used in the experiment operation: The plasmid used are pUC57-amilGFP and pUC57-Pprovoin5. Regarding the microorganisms, we only used E. coli which is commonly used and all the related experiments were conducted inside the laboratory under supervision only. Ethidium bromide, EB This used to be what is added to the electrophoresis gel, but considering the strong mutagenicity and toxicity, we replaced it with a safer nucleic acid dye. Autoclave Check the liquid level of the autoclave before using. Tap water should not be used or the components will be rusted. When the temperature is too high, the valve gate should be closed. An ultraviolet platform is used to observe the DNA stripes on the electrophoresis gel. When using it, do not stare at the light for too long in case it causes discomfort to the eyes. We use an alcohol lamp for high-temperature disinfection. Pay attention when moving the lamp, use both hands and do it slowly. Use the lamp cap to extinguish the fire instead of using other ways, such as blowing with your mouth.