Difference between revisions of "Team:UNILA LatAm/Contribution"

 
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<!-- # TODO: #6 Fix table caption font--><!-- # TODO: #7 Fix citations links font size--><html lang="en"><head><meta charset="utf-8"/><meta content="width=device-width,initial-scale=1" name="viewport"/><title>Contribution | iGEM UNILA_LatAm</title><script src="https://2020.igem.org/common/MathJax-2.5-latest/MathJax.js?config=TeX-AMS-MML_HTMLorMML"></script><link href="https://2021.igem.org/Template:UNILA_LatAm/css/contentCSS?action=raw&amp;ctype=text/css" rel="stylesheet"/></head><body><!-- # TODO: #6 Fix table caption font--><!-- # TODO: #7 Fix citations links font size--><nav class="navbar navbar-expand-xl fixed-top"><div class="container d-flex justify-content-between"><a class="navbar-brand d-lg-inline-block" href="https://2021.igem.org/Team:UNILA_LatAm"><img ;="" src="https://static.igem.org/mediawiki/2021/9/98/T--UNILA_LatAm--Logo.png" style="width:auto; height:100px ;position: relative !important; margin-top:15px; right: 300px"/></a><button aria-controls="navbarNav" 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href="https://2021.igem.org/Team:UNILA_LatAm/Contribution">Contribution</a></div></li><li class="nav-item dropdown"><a aria-expanded="false" aria-haspopup="true" class="nav-link dropdown-toggle" data-toggle="dropdown" href="#" id="navbarDryLabDropdown" role="button">DryLab</a><div aria-labelledby="navbarDryLabDropdown" class="dropdown-menu"><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Design">Design</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Model">Model</a></div></li><li class="nav-item dropdown"><a aria-expanded="false" aria-haspopup="true" class="nav-link dropdown-toggle" data-toggle="dropdown" href="#" id="navbarWetLabDropdown" role="button">WetLab</a><div aria-labelledby="navbarWetLabDropdown" class="dropdown-menu"><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Experiments">Experiments</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Results">Results</a><a class="dropdown-item" 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href="https://2021.igem.org/Team:UNILA_LatAm/Public_engagement_and_events">Public engagement and events</a></div></li><li class="nav-item dropdown"><a aria-expanded="false" aria-haspopup="true" class="nav-link dropdown-toggle" data-toggle="dropdown" href="#" id="navbarEducation &amp; public engagementDropdown" role="button">Education &amp; public engagement</a><div aria-labelledby="navbarEducation &amp; public engagementDropdown" class="dropdown-menu"><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/SynFronteras_Club">SynFronteras Club</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Survey_Leishmaniasis_Awareness">Survey Leishmaniasis Awareness</a></div></li><li class="nav-item dropdown"><a aria-expanded="false" aria-haspopup="true" class="nav-link dropdown-toggle" data-toggle="dropdown" href="#" id="navbarTeamDropdown" role="button">Team</a><div aria-labelledby="navbarTeamDropdown" class="dropdown-menu"><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Team">Team Members</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Attributions">Attributions</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Collaborations">Collaborations</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Partnership">Partnership</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Sponsors">Sponsors</a></div></li><li class="nav-item dropdown"><a aria-expanded="false" aria-haspopup="true" class="nav-link dropdown-toggle" data-toggle="dropdown" href="#" id="navbarAwardsDropdown" role="button">Awards</a><div aria-labelledby="navbarAwardsDropdown" class="dropdown-menu"><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Medal_Criteria">Medal Criteria</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Judging_Form">Judging Form</a></div></li></ul></div></div></nav><header class="d-flex justify-content-center align-items-center"><div class="container"><h1>Contribution</h1><p class="lead pl-1">help</p><hr class="my-4"/></div></header><main><div class="container"><div class="row"><div class="sidebar col-lg-3"><div class="nav" id="contents"><h5>Contents</h5><ul></ul></div></div><div class="content col-lg-9"><article><h1>Headings</h1><h2>Level 2 Heading</h2><h3>Level 3 Heading</h3><h4>Level 4 Heading</h4><h1>Emphasis</h1><p>This is regular text.</p><p><strong>This is bold text.</strong> <strong>This is also bold text.</strong></p><p><em>This is italic text.</em> _This is also italix text.*</p><p><em><strong>This text is bold and italic both.</strong></em> <em><strong>This text is bold and italic both.</strong></em></p><p><s>Strikethrough</s></p><h1>Lists</h1><ol><li>This is item one.</li><li>This is item two.</li><li>It's okay to number every item as 1.<ul><li>This is a nested list.</li><li>Use asterisk for an unordered list,</li><li>Further nesting?</li></ul><ul><li>Plus sign also works.</li></ul><ul><li>And so does minus.</li></ul></li><li><s>Birds aren't real.</s></li></ol><h1>Links</h1><p><a href="https://google.com">Google</a></p><p><a href="https://2021.igem.org/Team:UNILA_LatAm/Contribution/Link-goes-in-parenthesis">Text goes in square brackets</a></p><h1>Blockquote</h1><blockquote><p>This is a blockquote. This continues in the same paragraph.</p><p>To change the paragraph, you have to leave a line.</p></blockquote><blockquote><p>This is another blockquote.</p><p><strong>Pranav Ballaney, 2020</strong></p></blockquote><h1>Images</h1><p>This is a regular paragraph.</p><h4>This is a level 4 heading.</h4><p><a href="google.com">This is a link</a></p><div class="image"><img alt="Caption" src="https://static.igem.org/mediawiki/2021/3/3f/T--UNILA_LatAm--img--Description--josh-withers.jpg" style="width: 100%"/><p>Figure 1: Caption</p></div><p>Back to regular text.</p><h1>Tables</h1><p>This is a regular paragraph, which precedes the table. When you want to insert a table, indent one level back and specify the plugin. Then indent inside again and start writing the table.</p><table><caption id="table1captiongoeshere">Table 1: Caption goes here.</caption><thead><tr><th>This is</th><th>the table</th><th>header row</th></tr></thead><tbody><tr><td>1</td><td>2</td><td>3</td></tr><tr><td>4</td><td>5</td><td>6</td></tr></tbody></table><p>And when you're done, go back to the regular markdown filter.</p><p><strong>Example 2:</strong></p><table><thead><tr><th style="text-align:left">Left aligned</th><th style="text-align:center">Center aligned</th><th style="text-align:right">Right aligned</th></tr></thead><tbody><tr><td style="text-align:left">1</td><td style="text-align:center">2</td><td style="text-align:right">3</td></tr><tr><td style="text-align:left">4</td><td style="text-align:center">5</td><td style="text-align:right">6</td></tr></tbody></table><p><strong>Example 3:</strong></p><table><caption id="table3captiongoeshere">Table 3: Caption goes here.</caption><thead><tr><th></th><th colspan="2" style="text-align:center">Grouping</th></tr><tr><th>First Header</th><th style="text-align:center">Second Header</th><th style="text-align:right">Third Header</th></tr></thead><tbody><tr><td>Content</td><td colspan="2" style="text-align:center"><em>Long Cell</em></td></tr><tr><td>Content</td><td style="text-align:center"><strong>Cell</strong></td><td style="text-align:right">Cell</td></tr><tr><td>New section</td><td style="text-align:center">More</td><td style="text-align:right"><a href="https://google.com">Data</a></td></tr><tr><td>And more</td><td colspan="2" style="text-align:center">With an escaped '|'</td></tr></tbody></table><h1>Definitions</h1><p><dfn>Term ~ Definition</dfn></p><p>This can come <dfn>anywhere in ~ the text</dfn>.</p><h1>MathJax</h1><p>$$\int_{0}^{\infty} e^{-x^2} dx = \frac{\sqrt{\pi}}{2}$$</p><p>$$-\frac{\hbar^2}{2m}\nabla^2\psi + V\psi = E\psi$$</p><p>$$i\hbar\gamma^\mu\partial_\mu\psi = mc\psi$$</p><p>$$y_k = \frac{1}{\sqrt{N}}\sum_{n=0}^{N-1}x_n\omega_N^{kn}$$</p><h1>Citations</h1><p>In text citation for a research article with a DOI. <a href="#citation2">Rosano et al., 2019</a></p><p>In text citation for another research article with a DOI. <a href="#citation1">Allen &amp; Sheridan, 2015</a></p><p>In text citation for a book with no DOI. <a href="#citation3">Ingalls, 2013</a></p><p>In text citation for a website with institutional author. <a href="#citation4">TNAU Agritech Portal, n.d.</a></p><p>In text citation for a website with an author. <a href="#citation5">Author, n.d.</a></p></article><article id="references"><h1>References</h1><ol><li id="citation1"><p class="author">Allen, M. J., &amp; Sheridan, S. C. (2015).</p><cite>Mortality risks during extreme temperature events (ETEs) using a distributed lag non-linear model.</cite><p><span class="journalTitle">International Journal of Biometeorology</span> <span class="journalInfo">62(1), 57-67.</span></p><a class="in-text" href="https://doi.org/10.1007/s00484-015-1117-4" rel="noopener" target="_blank">CrossRef</a><a class="in-text" href="https://scholar.google.com/scholar?q=Mortality risks during extreme temperature events (ETEs) using a distributed lag non-linear model." rel="noopener" target="_blank">Google Scholar</a><a class="in-text" href="#intext1">Back to text</a></li><li id="citation2"><p class="author">Rosano, A., Bella, A., Gesualdo, F., Acampora, A., Pezzotti, P., Marchetti, S., ... &amp; Rizzo, C. (2019).</p><cite>Investigating the impact of influenza on excess mortality in all ages in Italy during recent seasons (2013/14-2016/17 seasons).</cite><p><span class="journalTitle">International Journal of Infectious Diseases</span> <span class="journalInfo">88, 127-134.</span></p><a class="in-text" href="https://doi.org/10.1016/j.ijid.2019.08.003" rel="noopener" target="_blank">CrossRef</a><a class="in-text" href="https://scholar.google.com/scholar?q=Investigating the impact of influenza on excess mortality in all ages in Italy during recent seasons (2013/14-2016/17 seasons)." rel="noopener" target="_blank">Google Scholar</a><a class="in-text" href="#intext2">Back to text</a></li><li id="citation3"><p class="author">Ingalls, B. P. (2013).</p><cite>Mathematical modeling in systems biology: An introduction.</cite><p><span class="details">MIT Press.</span></p><a class="in-text" href="https://books.google.co.in/books?id=OYr6AQAAQBAJ" rel="noopener" target="_blank">Google Books</a><a class="in-text" href="#intext3">Back to text</a></li><li id="citation4"><p class="author"></p><cite>Agriculture: Crop production: Sugarcane. TNAU Agritech Portal.</cite><p><span class="details">(March 15, 2019). Retrieved on June 22, 2020. from </span><a class="in-text" href="https://google.com" rel="noopener" target="_blank">https://google.com</a></p><a class="in-text" href="#intext4">Back to text</a></li><li id="citation5"><p class="author">Author Name. (n.d.).</p><cite>Agriculture: Crop production: Sugarcane. TNAU Agritech Portal.</cite><p><span class="details">Retrieved on June 22, 2020. from </span><a class="in-text" href="https://google.com" rel="noopener" target="_blank">https://google.com</a></p><a class="in-text" href="#intext5">Back to text</a></li></ol></article></div></div></div></main><footer><div class="container" id="contfoot"><div id="div1"><br/><p>BioPank is a project developed by</p><p>the Syn Fronteras team</p><br/><br/><br/><p id="text1">Contact</p><p>synfronteras@gmail.com</p></div><div id="div2"><p id="fow">Follow us!</p><img height="30px" src="https://static.igem.org/mediawiki/2021/c/c9/T--UNILA_LatAM--fb.png" weight="30px"/><a href="https://www.facebook.com/synfronteras/">/synfronteras<br/></a><img height="30px" src="https://static.igem.org/mediawiki/2021/2/2c/T--UNILA_LatAM--ig.png" weight="30px"/><a href="https://www.instagram.com/igem_synfronteras/">@igem_synfronteras<br/></a><img height="30px" src="https://static.igem.org/mediawiki/2021/1/19/T--UNILA_LatAM--in.png" weight="30px"/><a href="https://br.linkedin.com/in/synfronteras-igem-team-77a7891ba">SynFronteras iGEM Team<br/></a><img height="30px" src="https://static.igem.org/mediawiki/2021/2/27/T--UNILA_LatAM--tw.png" weight="30px"/><a href="https://twitter.com/synfronteras">@synfronteras<br/></a><img height="30px" src="https://static.igem.org/mediawiki/2021/0/02/T--UNILA_LatAM--yt.png" weight="30px"/><a href="https://www.youtube.com/channel/UCHGAVNbPReEbzdEHeansZQg">SynFronteras-UNILA<br/></a></div></div></footer><script src="https://2021.igem.org/Template:UNILA_LatAm/content-bundleJS?action=raw&amp;ctype=text/javascript"></script></body></html>
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<!-- # TODO: #6 Fix table caption font--><!-- # TODO: #7 Fix citations links font size--><html lang="en"><head><meta charset="utf-8"/><meta content="width=device-width,initial-scale=1" name="viewport"/><title>Contribution | iGEM UNILA_LatAm</title><script src="https://2020.igem.org/common/MathJax-2.5-latest/MathJax.js?config=TeX-AMS-MML_HTMLorMML"></script><link href="https://2021.igem.org/Template:UNILA_LatAm/css/contentCSS?action=raw&amp;ctype=text/css" rel="stylesheet"/></head><body><!-- # TODO: #6 Fix table caption font--><!-- # TODO: #7 Fix citations links font size--><nav class="navbar navbar-expand-xl fixed-top"><div class="container d-flex justify-content-between"><a class="navbar-brand d-lg-inline-block" href="https://2021.igem.org/Team:UNILA_LatAm"><img ;="" src="https://static.igem.org/mediawiki/2021/9/98/T--UNILA_LatAm--Logo.png" style="width:auto; height:100px ;position: relative !important; margin-top:15px; right: 300px"/></a><button aria-controls="navbarNav" aria-expanded="false" aria-label="Toggle navigation" class="navbar-toggler" data-target="#navbarNav" data-toggle="collapse" type="button"><span class="navbar-toggler-icon"></span></button><div class="collapse navbar-collapse" id="navbarNav"><ul class="navbar-nav ml-auto"><li class="nav-item dropdown"><a aria-expanded="false" aria-haspopup="true" class="nav-link dropdown-toggle" data-toggle="dropdown" href="#" id="navbarProjectDropdown" role="button">Project</a><div aria-labelledby="navbarProjectDropdown" class="dropdown-menu"><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Description">Description</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Design_Overview">Design Overview</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Background">Background</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Engineering">Engineering</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Contribution">Contribution</a></div></li><li class="nav-item dropdown"><a aria-expanded="false" aria-haspopup="true" class="nav-link dropdown-toggle" data-toggle="dropdown" href="#" id="navbarDryLabDropdown" role="button">DryLab</a><div aria-labelledby="navbarDryLabDropdown" class="dropdown-menu"><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Design">Design</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Model">Model</a></div></li><li class="nav-item dropdown"><a aria-expanded="false" aria-haspopup="true" class="nav-link dropdown-toggle" data-toggle="dropdown" href="#" id="navbarWetLabDropdown" role="button">WetLab</a><div aria-labelledby="navbarWetLabDropdown" class="dropdown-menu"><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Experiments">Experiments</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Results">Results</a><a class="dropdown-item" 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class="sidebar col-lg-3"><div class="nav" id="contents"><h5>Contents</h5><ul></ul></div></div><div class="content col-lg-9"><article><h1>Parts</h1><p>We added information to two parts in the registry:</p><p><a href="http://parts.igem.org/Part:BBa_K2486108">http://parts.igem.org/Part:BBa_K2486108</a>.<br/><a href="http://parts.igem.org/Part:BBa_K2577001">http://parts.igem.org/Part:BBa_K2577001</a>.</p><p>Both parts encodes Dermaseptins, a type of Antimicrobial Peptide (AMP) derived from frogs. We included information from the literature for use and biology of the parts.</p><h1>How to fund</h1><p>Funding is a challenge for all iGEM teams, but for teams from developing countries, it's a challenge even harder to complete. That is why the SynFronteras Team elaborated a report that can help teams who are struggling with this topic or may be a little lost on how to begin. You can find the document <a href="https://static.igem.org/mediawiki/2021/b/bd/T--UNILA_LatAM--How_to_fund.pdf">here</a>.</p><h1>Cell Lysis Mechanisms Table</h1><p>In the partnership with team FCB-UANL, we created a table of potential cell lysis mechanisms for Bacillus subtilis. This table can be used by future teams that want to work with this chassis. You can access the table <a href="https://zenodo.org/record/5553126#.YXBAK-rMJH5">here</a>.</p><h1>Software</h1><p>We chose to work with a ToeHold RNA Switch in our project, but the available platforms for the Toehold's design did not cover all the aspects necessary for us. That's why we created the python-based Carpincho Tool Toehold Designer to design our Toehold Switches based on thermodynamic values using the NUPACK package (<a href="https://github.com/igemsoftware2021/CarpinchoToeholdDesigner">check here</a>).</p></article></div></div></div></main><footer><div class="container" id="contfoot"><div id="div1"><p id="descri">A Project by</p><br/><img height="100px" src="https://static.igem.org/mediawiki/2021/d/dc/T--UNILA_LatAM--LogoV3.png" weight="100px"/><br/><br/><p id="text1">Contact</p><p>synfronteras@gmail.com</p></div><div id="div2"><p id="spon">Sponsor</p><br/><a href="https://www.itaipu.gov.br/"><img height="150px" id="spons" src="https://static.igem.org/mediawiki/2021/8/89/T--UNILA_LatAM--LogoIPH.png" weight="150px"/></a></div><div id="div3"><p id="fow">Follow us!</p><br/><img height="30px" id="aa" src="https://static.igem.org/mediawiki/2021/c/c9/T--UNILA_LatAM--fb.png" weight="30px"/><a href="https://www.facebook.com/synfronteras/">/synfronteras<br/></a><img height="30px" src="https://static.igem.org/mediawiki/2021/2/2c/T--UNILA_LatAM--ig.png" weight="30px"/><a href="https://www.instagram.com/igem_synfronteras/">@igem_synfronteras<br/></a><img height="30px" src="https://static.igem.org/mediawiki/2021/1/19/T--UNILA_LatAM--in.png" weight="30px"/><a href="https://br.linkedin.com/in/synfronteras-igem-team-77a7891ba">SynFronteras iGEM Team<br/></a><img height="30px" src="https://static.igem.org/mediawiki/2021/2/27/T--UNILA_LatAM--tw.png" weight="30px"/><a href="https://twitter.com/synfronteras">@synfronteras<br/></a><img height="30px" src="https://static.igem.org/mediawiki/2021/0/02/T--UNILA_LatAM--yt.png" weight="30px"/><a href="https://www.youtube.com/channel/UCHGAVNbPReEbzdEHeansZQg">SynFronteras-UNILA<br/></a></div></div></footer><script src="https://2021.igem.org/Template:UNILA_LatAm/content-bundleJS?action=raw&amp;ctype=text/javascript"></script></body></html>

Latest revision as of 23:29, 17 December 2021

Contribution | iGEM UNILA_LatAm

Contribution


Parts

We added information to two parts in the registry:

http://parts.igem.org/Part:BBa_K2486108.
http://parts.igem.org/Part:BBa_K2577001.

Both parts encodes Dermaseptins, a type of Antimicrobial Peptide (AMP) derived from frogs. We included information from the literature for use and biology of the parts.

How to fund

Funding is a challenge for all iGEM teams, but for teams from developing countries, it's a challenge even harder to complete. That is why the SynFronteras Team elaborated a report that can help teams who are struggling with this topic or may be a little lost on how to begin. You can find the document here.

Cell Lysis Mechanisms Table

In the partnership with team FCB-UANL, we created a table of potential cell lysis mechanisms for Bacillus subtilis. This table can be used by future teams that want to work with this chassis. You can access the table here.

Software

We chose to work with a ToeHold RNA Switch in our project, but the available platforms for the Toehold's design did not cover all the aspects necessary for us. That's why we created the python-based Carpincho Tool Toehold Designer to design our Toehold Switches based on thermodynamic values using the NUPACK package (check here).