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<!-- # TODO: #6 Fix table caption font--><!-- # TODO: #7 Fix citations links font size--><html lang="en"><head><meta charset="utf-8"/><meta content="width=device-width,initial-scale=1" name="viewport"/><title>Parts | iGEM UNILA_LatAm</title><script src="https://2020.igem.org/common/MathJax-2.5-latest/MathJax.js?config=TeX-AMS-MML_HTMLorMML"></script><link href="https://2021.igem.org/Template:UNILA_LatAm/css/contentCSS?action=raw&amp;ctype=text/css" rel="stylesheet"/></head><body><!-- # TODO: #6 Fix table caption font--><!-- # TODO: #7 Fix citations links font size--><nav class="navbar navbar-expand-xl fixed-top"><div class="container d-flex justify-content-between"><a class="navbar-brand d-lg-inline-block" href="https://2021.igem.org/Team:UNILA_LatAm"><span>iGEM </span>UNILA_LatAm</a><button aria-controls="navbarNav" aria-expanded="false" aria-label="Toggle navigation" class="navbar-toggler" data-target="#navbarNav" data-toggle="collapse" type="button"><span class="navbar-toggler-icon"></span></button><div class="collapse navbar-collapse" id="navbarNav"><ul class="navbar-nav ml-auto"><li class="nav-item dropdown"><a aria-expanded="false" aria-haspopup="true" class="nav-link dropdown-toggle" data-toggle="dropdown" href="#" id="navbarOverviewDropdown" role="button">Overview</a><div aria-labelledby="navbarOverviewDropdown" class="dropdown-menu"><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Description">Description</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Design">Design</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Background">Background</a></div></li><li class="nav-item dropdown"><a aria-expanded="false" aria-haspopup="true" class="nav-link dropdown-toggle" data-toggle="dropdown" href="#" id="navbarProjectDropdown" role="button">Project</a><div aria-labelledby="navbarProjectDropdown" class="dropdown-menu"><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Paratransgenesis">Paratransgenesis</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Description1">Description1</a></div></li><li class="nav-item dropdown"><a aria-expanded="false" aria-haspopup="true" class="nav-link dropdown-toggle" data-toggle="dropdown" href="#" id="navbarDryLabDropdown" role="button">DryLab</a><div aria-labelledby="navbarDryLabDropdown" class="dropdown-menu"><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Design1">Design1</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Toehold_Switch">Toehold Switch</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Modeling">Modeling</a></div></li><li class="nav-item dropdown"><a aria-expanded="false" aria-haspopup="true" class="nav-link dropdown-toggle" data-toggle="dropdown" href="#" id="navbarWetLabDropdown" role="button">WetLab</a><div aria-labelledby="navbarWetLabDropdown" class="dropdown-menu"><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Demonstrate">Demonstrate</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Notebook">Notebook</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Protocols">Protocols</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Parts">Parts</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Safety">Safety</a></div></li><li class="nav-item dropdown"><a aria-expanded="false" aria-haspopup="true" class="nav-link dropdown-toggle" data-toggle="dropdown" href="#" id="navbarHuman PracticesDropdown" role="button">Human Practices</a><div aria-labelledby="navbarHuman PracticesDropdown" class="dropdown-menu"><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Integrated_Human_Practices">Integrated Human Practices</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Education_&amp;_Public_Engagement">Education &amp; Public Engagement</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Implementation">Implementation</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Fighting_Underreporting">Fighting Underreporting</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Funding">Funding</a></div></li><li class="nav-item dropdown"><a aria-expanded="false" aria-haspopup="true" class="nav-link dropdown-toggle" data-toggle="dropdown" href="#" id="navbarTeamDropdown" role="button">Team</a><div aria-labelledby="navbarTeamDropdown" class="dropdown-menu"><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Team">Team</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Attributions">Attributions</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Collaborations">Collaborations</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Sponsors">Sponsors</a></div></li><li class="nav-item dropdown"><a aria-expanded="false" aria-haspopup="true" class="nav-link dropdown-toggle" data-toggle="dropdown" href="#" id="navbarAwardsDropdown" role="button">Awards</a><div aria-labelledby="navbarAwardsDropdown" class="dropdown-menu"><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Medal_criteria">Medal criteria</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Special_prizes">Special prizes</a></div></li></ul></div><div class="d-flex" id="themeSwitchWrapper"><i class="far fa-sun"></i><div id="themeSwitch"><label class="switch" for="themeSwitchInput"><input id="themeSwitchInput" type="checkbox"/><span class="slider round"></span></label></div><i class="far fa-moon"></i></div></div></nav><header class="d-flex justify-content-center align-items-center"><div class="container"><h1>Parts</h1><p class="lead pl-1">asdasd.</p><hr class="my-4"/></div></header><main><div class="container"><div class="row"><div class="sidebar col-lg-3"><div class="nav" id="contents"><h5>Contents</h5><ul></ul></div></div><div class="content col-lg-9"><article><h1>Headings</h1><h2>Level 2 Heading</h2><h3>Level 3 Heading</h3><h4>Level 4 Heading</h4><h1>Emphasis</h1><p>This is regular text.</p><p><strong>This is bold text.</strong> <strong>This is also bold text.</strong></p><p><em>This is italic text.</em> _This is also italix text.*</p><p><em><strong>This text is bold and italic both.</strong></em> <em><strong>This text is bold and italic both.</strong></em></p><p><s>Strikethrough</s></p><h1>Lists</h1><ol><li>This is item one.</li><li>This is item two.</li><li>It's okay to number every item as 1.<ul><li>This is a nested list.</li><li>Use asterisk for an unordered list,</li><li>Further nesting?</li></ul><ul><li>Plus sign also works.</li></ul><ul><li>And so does minus.</li></ul></li><li><s>Birds aren't real.</s></li></ol><h1>Links</h1><p><a href="https://google.com">Google</a></p><p><a href="https://2021.igem.org/Team:UNILA_LatAm/Parts/Link-goes-in-parenthesis">Text goes in square brackets</a></p><h1>Blockquote</h1><blockquote><p>This is a blockquote. This continues in the same paragraph.</p><p>To change the paragraph, you have to leave a line.</p></blockquote><blockquote><p>This is another blockquote.</p><p><strong>Pranav Ballaney, 2020</strong></p></blockquote><h1>Images</h1><p>This is a regular paragraph.</p><h4>This is a level 4 heading.</h4><p><a href="google.com">This is a link</a></p><div class="image"><img alt="Caption" src="https://static.igem.org/mediawiki/2021/3/3f/T--UNILA_LatAm--img--Description--josh-withers.jpg" style="width: 100%"/><p>Figure 1: Caption</p></div><p>Back to regular text.</p><h1>Tables</h1><p>This is a regular paragraph, which precedes the table. When you want to insert a table, indent one level back and specify the plugin. Then indent inside again and start writing the table.</p><table><caption id="table1captiongoeshere">Table 1: Caption goes here.</caption><thead><tr><th>This is</th><th>the table</th><th>header row</th></tr></thead><tbody><tr><td>1</td><td>2</td><td>3</td></tr><tr><td>4</td><td>5</td><td>6</td></tr></tbody></table><p>And when you're done, go back to the regular markdown filter.</p><p><strong>Example 2:</strong></p><table><thead><tr><th style="text-align:left">Left aligned</th><th style="text-align:center">Center aligned</th><th style="text-align:right">Right aligned</th></tr></thead><tbody><tr><td style="text-align:left">1</td><td style="text-align:center">2</td><td style="text-align:right">3</td></tr><tr><td style="text-align:left">4</td><td style="text-align:center">5</td><td style="text-align:right">6</td></tr></tbody></table><p><strong>Example 3:</strong></p><table><caption id="table3captiongoeshere">Table 3: Caption goes here.</caption><thead><tr><th></th><th colspan="2" style="text-align:center">Grouping</th></tr><tr><th>First Header</th><th style="text-align:center">Second Header</th><th style="text-align:right">Third Header</th></tr></thead><tbody><tr><td>Content</td><td colspan="2" style="text-align:center"><em>Long Cell</em></td></tr><tr><td>Content</td><td style="text-align:center"><strong>Cell</strong></td><td style="text-align:right">Cell</td></tr><tr><td>New section</td><td style="text-align:center">More</td><td style="text-align:right"><a href="https://google.com">Data</a></td></tr><tr><td>And more</td><td colspan="2" style="text-align:center">With an escaped '|'</td></tr></tbody></table><h1>Definitions</h1><p><dfn>Term ~ Definition</dfn></p><p>This can come <dfn>anywhere in ~ the text</dfn>.</p><h1>MathJax</h1><p>$$\int_{0}^{\infty} e^{-x^2} dx = \frac{\sqrt{\pi}}{2}$$</p><p>$$-\frac{\hbar^2}{2m}\nabla^2\psi + V\psi = E\psi$$</p><p>$$i\hbar\gamma^\mu\partial_\mu\psi = mc\psi$$</p><p>$$y_k = \frac{1}{\sqrt{N}}\sum_{n=0}^{N-1}x_n\omega_N^{kn}$$</p><h1>Citations</h1><p>In text citation for a research article with a DOI. <a href="#citation2">Rosano et al., 2019</a></p><p>In text citation for another research article with a DOI. <a href="#citation1">Allen &amp; Sheridan, 2015</a></p><p>In text citation for a book with no DOI. <a href="#citation3">Ingalls, 2013</a></p><p>In text citation for a website with institutional author. <a href="#citation4">TNAU Agritech Portal, n.d.</a></p><p>In text citation for a website with an author. <a href="#citation5">Author, n.d.</a></p></article><article id="references"><h1>References</h1><ol><li id="citation1"><p class="author">Allen, M. J., &amp; Sheridan, S. C. (2015).</p><cite>Mortality risks during extreme temperature events (ETEs) using a distributed lag non-linear model.</cite><p><span class="journalTitle">International Journal of Biometeorology</span> <span class="journalInfo">62(1), 57-67.</span></p><a class="in-text" href="https://doi.org/10.1007/s00484-015-1117-4" rel="noopener" target="_blank">CrossRef</a><a class="in-text" href="https://scholar.google.com/scholar?q=Mortality risks during extreme temperature events (ETEs) using a distributed lag non-linear model." rel="noopener" target="_blank">Google Scholar</a><a class="in-text" href="#intext1">Back to text</a></li><li id="citation2"><p class="author">Rosano, A., Bella, A., Gesualdo, F., Acampora, A., Pezzotti, P., Marchetti, S., ... &amp; Rizzo, C. (2019).</p><cite>Investigating the impact of influenza on excess mortality in all ages in Italy during recent seasons (2013/14-2016/17 seasons).</cite><p><span class="journalTitle">International Journal of Infectious Diseases</span> <span class="journalInfo">88, 127-134.</span></p><a class="in-text" href="https://doi.org/10.1016/j.ijid.2019.08.003" rel="noopener" target="_blank">CrossRef</a><a class="in-text" href="https://scholar.google.com/scholar?q=Investigating the impact of influenza on excess mortality in all ages in Italy during recent seasons (2013/14-2016/17 seasons)." rel="noopener" target="_blank">Google Scholar</a><a class="in-text" href="#intext2">Back to text</a></li><li id="citation3"><p class="author">Ingalls, B. P. (2013).</p><cite>Mathematical modeling in systems biology: An introduction.</cite><p><span class="details">MIT Press.</span></p><a class="in-text" href="https://books.google.co.in/books?id=OYr6AQAAQBAJ" rel="noopener" target="_blank">Google Books</a><a class="in-text" href="#intext3">Back to text</a></li><li id="citation4"><p class="author"></p><cite>Agriculture: Crop production: Sugarcane. TNAU Agritech Portal.</cite><p><span class="details">(March 15, 2019). Retrieved on June 22, 2020. from </span><a class="in-text" href="https://google.com" rel="noopener" target="_blank">https://google.com</a></p><a class="in-text" href="#intext4">Back to text</a></li><li id="citation5"><p class="author">Author Name. (n.d.).</p><cite>Agriculture: Crop production: Sugarcane. TNAU Agritech Portal.</cite><p><span class="details">Retrieved on June 22, 2020. from </span><a class="in-text" href="https://google.com" rel="noopener" target="_blank">https://google.com</a></p><a class="in-text" href="#intext5">Back to text</a></li></ol></article></div></div></div></main><footer><div class="container"><p>Sample template built using the iGEM Wiki Starter Pack by BITS Goa.</p><p>Code released under the MIT license.</p><p>Based on <a href="https://getbootstrap.com">Bootstrap</a> and themes <a href="https://bootswatch.com/flatly/">Flatly</a> and <a href="https://bootswatch.com/darkly/">Darkly</a> from <a href="https://bootswatch.com/">Bootswatch</a>.</p><p>Some content from the <a href="https://2020.igem.org/Team:Example">iGEM Example Wiki</a>. Images from <a href="https://unsplash.com">Unsplash</a>. Web fonts from <a href="https://fonts.google.com">Google</a>.</p></div></footer><script src="https://2021.igem.org/Template:UNILA_LatAm/content-bundleJS?action=raw&amp;ctype=text/javascript"></script></body></html>
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<!-- # TODO: #6 Fix table caption font--><!-- # TODO: #7 Fix citations links font size--><html lang="en"><head><meta charset="utf-8"/><meta content="width=device-width,initial-scale=1" name="viewport"/><title>Parts | iGEM UNILA_LatAm</title><script src="https://2020.igem.org/common/MathJax-2.5-latest/MathJax.js?config=TeX-AMS-MML_HTMLorMML"></script><link href="https://2021.igem.org/Template:UNILA_LatAm/css/contentCSS?action=raw&amp;ctype=text/css" rel="stylesheet"/></head><body><!-- # TODO: #6 Fix table caption font--><!-- # TODO: #7 Fix citations links font size--><nav class="navbar navbar-expand-xl fixed-top"><div class="container d-flex justify-content-between"><a class="navbar-brand d-lg-inline-block" href="https://2021.igem.org/Team:UNILA_LatAm"><img ;="" src="https://static.igem.org/mediawiki/2021/9/98/T--UNILA_LatAm--Logo.png" style="width:auto; height:100px ;position: relative !important; margin-top:15px; right: 300px"/></a><button aria-controls="navbarNav" aria-expanded="false" aria-label="Toggle navigation" class="navbar-toggler" data-target="#navbarNav" data-toggle="collapse" type="button"><span class="navbar-toggler-icon"></span></button><div class="collapse navbar-collapse" id="navbarNav"><ul class="navbar-nav ml-auto"><li class="nav-item dropdown"><a aria-expanded="false" aria-haspopup="true" class="nav-link dropdown-toggle" data-toggle="dropdown" href="#" id="navbarProjectDropdown" role="button">Project</a><div aria-labelledby="navbarProjectDropdown" class="dropdown-menu"><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Description">Description</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Design_Overview">Design Overview</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Background">Background</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Engineering">Engineering</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Contribution">Contribution</a></div></li><li class="nav-item dropdown"><a aria-expanded="false" aria-haspopup="true" class="nav-link dropdown-toggle" data-toggle="dropdown" href="#" id="navbarDryLabDropdown" role="button">DryLab</a><div aria-labelledby="navbarDryLabDropdown" class="dropdown-menu"><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Design">Design</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Model">Model</a></div></li><li class="nav-item dropdown"><a aria-expanded="false" aria-haspopup="true" class="nav-link dropdown-toggle" data-toggle="dropdown" href="#" id="navbarWetLabDropdown" role="button">WetLab</a><div aria-labelledby="navbarWetLabDropdown" class="dropdown-menu"><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Experiments">Experiments</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Results">Results</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Notebook">Notebook</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Protocols">Protocols</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Parts">Parts</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Safety">Safety</a></div></li><li class="nav-item dropdown"><a aria-expanded="false" aria-haspopup="true" class="nav-link dropdown-toggle" data-toggle="dropdown" href="#" id="navbarHuman PracticesDropdown" role="button">Human Practices</a><div aria-labelledby="navbarHuman PracticesDropdown" class="dropdown-menu"><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Human_Practices">Introduction</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Sustainability">Sustainability</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Implementation">Implementation</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Fighting_Underreporting">Fighting Underreporting</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Integrated_Human_Practices">Integrated Human Practices</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Education">Education</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Inclusivity">Inclusivity</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Communication">Communication</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Funding">Funding</a></div></li><li class="nav-item dropdown"><a aria-expanded="false" aria-haspopup="true" class="nav-link dropdown-toggle" data-toggle="dropdown" href="#" id="navbarTeamDropdown" role="button">Team</a><div aria-labelledby="navbarTeamDropdown" class="dropdown-menu"><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Team">Team Members</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Attributions">Attributions</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Collaborations">Collaborations</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Partnership">Partnership</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Sponsors">Sponsors</a></div></li><li class="nav-item dropdown"><a aria-expanded="false" aria-haspopup="true" class="nav-link dropdown-toggle" data-toggle="dropdown" href="#" id="navbarAwardsDropdown" role="button">Awards</a><div aria-labelledby="navbarAwardsDropdown" class="dropdown-menu"><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Medal_Criteria">Medal Criteria</a></div></li></ul></div></div></nav><header class="d-flex justify-content-center align-items-center"><div class="container"><h1>Parts</h1><p class="lead pl-1"></p><hr class="my-4"/></div></header><main><div class="container"><div class="row"><div class="sidebar col-lg-3"><div class="nav" id="contents"><h5>Contents</h5><ul></ul></div></div><div class="content col-lg-9"><article><h1>Overview</h1><p>Our designed parts from the four modules were classified into basic and composite parts, responsible for composing our strategy of paratransgenesis in the fight against leishmaniasis transmission. The basic parts are sequences designed during the development of our system and are used to form composite parts. Both classes of sequences can compose a Device that conducts an operation on the chassis. Also, we present the Kill the Leishmania Collection, a collection of our favorite parts that can be applied in approaches beyond paratransgenesis.</p><p>Although we could not test all parts, we provided detailed information or modeling of parts not evaluated in the Wet Lab. Due to COVID-19, we had difficulty accessing the laboratory, which made it impossible for us to evaluate all the drawn parts. However, we were able to assemble the parts BBa_K4075008 and BBa_K4075013, but transform only the part BBa_K4075008 into E. coli BL21(DE3) to evaluate the functionality of proDRS-N1.</p><h2>Part Collection</h2><p>This season our main focus was testing the Elimination Module, which motivated us to design a new strategy for modularizing the toxicity of AMPs. That's why we offer <strong>Kill the Leishmania Collection</strong>, a collection of parts that gathers AMPs and pro-peptides. These parts can drive other applications with different AMPs, as described by the Paratransgenesis Collection (from iGEM USP 2017) or Antimicrobial Peptides Collection (from iGEM NEU 2017)</p><h2>PARTS TABLE</h2><table><thead><tr><th>Name</th><th>Description</th><th>Designer</th><th>Class</th><th>Parts Collection</th></tr></thead><tbody><tr><td><a href="http://parts.igem.org/Part:BBa_K4075555">BBa_K4075555</a></td><td>Germination Measurement Device for B. subtilis</td><td>Samuel Chagas</td><td>Device</td><td></td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K4075001">BBa_K4075001</a></td><td>Natural Dermaseptin-H3 acidic pro-peptide</td><td>Giulio Braatz</td><td>Basic</td><td></td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K4075002">BBa_K4075002</a></td><td>Synthetic acidic pro-peptide</td><td>Júnior et al. [1]</td><td>Basic</td><td></td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K4075003">BBa_K4075003</a></td><td>Natural Dermaseptin-S1 acidic pro-peptide</td><td>Giulio Braatz</td><td>Basic</td><td></td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K4075004">BBa_K4075004</a></td><td>AMP DRS-N1</td><td>Giulio Braatz</td><td>Basic</td><td></td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K4075005">BBa_K4075005</a></td><td>AMP CAM-W</td><td>Giulio Braatz</td><td>Basic</td><td></td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K4075006">BBa_K4075006</a></td><td>AMP DRS-S1</td><td>Giulio Braatz</td><td>Basic</td><td></td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K4075007">BBa_K4075007</a></td><td>AMP DRS-H3</td><td>Giulio Braatz</td><td>Basic</td><td></td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K4075014">BBa_K4075014</a></td><td>proDRS-N1</td><td>Giulio Braatz</td><td>Composite</td><td>Collection</td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K4075015">BBa_K4075015</a></td><td>proCAM-W</td><td>Giulio Braatz</td><td>Composite</td><td>Collection</td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K4075016">BBa_K4075016</a></td><td>proDRS-S1</td><td>Giulio Braatz</td><td>Composite</td><td>Collection</td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K4075017">BBa_K4075017</a></td><td>proDRS-H3</td><td>Giulio Braatz</td><td>Composite</td><td>Collection</td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K4075018">BBa_K4075018</a></td><td>proE10+DRS-N1</td><td>Giulio Braatz</td><td>Composite</td><td>Collection</td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K4075009">BBa_K4075009</a></td><td>PT7_LacO+OmpA</td><td>Matheus Araujo</td><td>Composite</td><td></td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K4075008">BBa_K4075008</a></td><td>PT7_LacO+proDRS-N1+Term E. coli</td><td>Matheus Araujo</td><td>Device</td><td></td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K4075013">BBa_K4075013</a></td><td>PT7_LacO+mCherry+Term E. coli</td><td>Matheus Araujo</td><td>Device</td><td></td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K4075100">BBa_K4075100</a></td><td>C-Split Cas12a(Cpf1) with nMag for B. subtilis</td><td>Samuel Chagas</td><td>Basic</td><td></td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K4075100">BBa_K4075101</a></td><td>N-Split Cas12a(Cpf1) with pMag for B. subtilis</td><td>Samuel Chagas</td><td>Basic</td><td></td></tr></tbody></table></article></div></div></div></main><footer><div class="container" id="contfoot"><div id="div1"><p id="descri">A Project by</p><br/><img height="100px" src="https://static.igem.org/mediawiki/2021/d/dc/T--UNILA_LatAM--LogoV3.png" weight="100px"/><br/><br/><p id="text1">Contact</p><p>synfronteras@gmail.com</p></div><div id="div2"><p id="spon">Sponsor</p><br/><a href="https://www.itaipu.gov.br/"><img height="150px" id="spons" src="https://static.igem.org/mediawiki/2021/8/89/T--UNILA_LatAM--LogoIPH.png" weight="150px"/></a></div><div id="div3"><p id="fow">Follow us!</p><br/><img height="30px" id="aa" src="https://static.igem.org/mediawiki/2021/c/c9/T--UNILA_LatAM--fb.png" weight="30px"/><a href="https://www.facebook.com/synfronteras/">/synfronteras<br/></a><img height="30px" src="https://static.igem.org/mediawiki/2021/2/2c/T--UNILA_LatAM--ig.png" weight="30px"/><a href="https://www.instagram.com/igem_synfronteras/">@igem_synfronteras<br/></a><img height="30px" src="https://static.igem.org/mediawiki/2021/1/19/T--UNILA_LatAM--in.png" weight="30px"/><a href="https://br.linkedin.com/in/synfronteras-igem-team-77a7891ba">SynFronteras iGEM Team<br/></a><img height="30px" src="https://static.igem.org/mediawiki/2021/2/27/T--UNILA_LatAM--tw.png" weight="30px"/><a href="https://twitter.com/synfronteras">@synfronteras<br/></a><img height="30px" src="https://static.igem.org/mediawiki/2021/0/02/T--UNILA_LatAM--yt.png" weight="30px"/><a href="https://www.youtube.com/channel/UCHGAVNbPReEbzdEHeansZQg">SynFronteras-UNILA<br/></a></div></div></footer><script src="https://2021.igem.org/Template:UNILA_LatAm/content-bundleJS?action=raw&amp;ctype=text/javascript"></script></body></html>

Latest revision as of 23:11, 17 December 2021

Parts | iGEM UNILA_LatAm

Parts


Overview

Our designed parts from the four modules were classified into basic and composite parts, responsible for composing our strategy of paratransgenesis in the fight against leishmaniasis transmission. The basic parts are sequences designed during the development of our system and are used to form composite parts. Both classes of sequences can compose a Device that conducts an operation on the chassis. Also, we present the Kill the Leishmania Collection, a collection of our favorite parts that can be applied in approaches beyond paratransgenesis.

Although we could not test all parts, we provided detailed information or modeling of parts not evaluated in the Wet Lab. Due to COVID-19, we had difficulty accessing the laboratory, which made it impossible for us to evaluate all the drawn parts. However, we were able to assemble the parts BBa_K4075008 and BBa_K4075013, but transform only the part BBa_K4075008 into E. coli BL21(DE3) to evaluate the functionality of proDRS-N1.

Part Collection

This season our main focus was testing the Elimination Module, which motivated us to design a new strategy for modularizing the toxicity of AMPs. That's why we offer Kill the Leishmania Collection, a collection of parts that gathers AMPs and pro-peptides. These parts can drive other applications with different AMPs, as described by the Paratransgenesis Collection (from iGEM USP 2017) or Antimicrobial Peptides Collection (from iGEM NEU 2017)

PARTS TABLE

NameDescriptionDesignerClassParts Collection
BBa_K4075555Germination Measurement Device for B. subtilisSamuel ChagasDevice
BBa_K4075001Natural Dermaseptin-H3 acidic pro-peptideGiulio BraatzBasic
BBa_K4075002Synthetic acidic pro-peptideJúnior et al. [1]Basic
BBa_K4075003Natural Dermaseptin-S1 acidic pro-peptideGiulio BraatzBasic
BBa_K4075004AMP DRS-N1Giulio BraatzBasic
BBa_K4075005AMP CAM-WGiulio BraatzBasic
BBa_K4075006AMP DRS-S1Giulio BraatzBasic
BBa_K4075007AMP DRS-H3Giulio BraatzBasic
BBa_K4075014proDRS-N1Giulio BraatzCompositeCollection
BBa_K4075015proCAM-WGiulio BraatzCompositeCollection
BBa_K4075016proDRS-S1Giulio BraatzCompositeCollection
BBa_K4075017proDRS-H3Giulio BraatzCompositeCollection
BBa_K4075018proE10+DRS-N1Giulio BraatzCompositeCollection
BBa_K4075009PT7_LacO+OmpAMatheus AraujoComposite
BBa_K4075008PT7_LacO+proDRS-N1+Term E. coliMatheus AraujoDevice
BBa_K4075013PT7_LacO+mCherry+Term E. coliMatheus AraujoDevice
BBa_K4075100C-Split Cas12a(Cpf1) with nMag for B. subtilisSamuel ChagasBasic
BBa_K4075101N-Split Cas12a(Cpf1) with pMag for B. subtilisSamuel ChagasBasic