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| + | <!-- # TODO: #6 Fix table caption font--><!-- # TODO: #7 Fix citations links font size--><html lang="en"><head><meta charset="utf-8"/><meta content="width=device-width,initial-scale=1" name="viewport"/><title>Parts | iGEM UNILA_LatAm</title><script src="https://2020.igem.org/common/MathJax-2.5-latest/MathJax.js?config=TeX-AMS-MML_HTMLorMML"></script><link href="https://2021.igem.org/Template:UNILA_LatAm/css/contentCSS?action=raw&ctype=text/css" rel="stylesheet"/></head><body><!-- # TODO: #6 Fix table caption font--><!-- # TODO: #7 Fix citations links font size--><nav class="navbar navbar-expand-xl fixed-top"><div class="container d-flex justify-content-between"><a class="navbar-brand d-lg-inline-block" href="https://2021.igem.org/Team:UNILA_LatAm"><img ;="" src="https://static.igem.org/mediawiki/2021/9/98/T--UNILA_LatAm--Logo.png" style="width:auto; height:100px ;position: relative !important; margin-top:15px; right: 300px"/></a><button aria-controls="navbarNav" aria-expanded="false" aria-label="Toggle navigation" class="navbar-toggler" data-target="#navbarNav" data-toggle="collapse" type="button"><span class="navbar-toggler-icon"></span></button><div class="collapse navbar-collapse" id="navbarNav"><ul class="navbar-nav ml-auto"><li class="nav-item dropdown"><a aria-expanded="false" aria-haspopup="true" class="nav-link dropdown-toggle" data-toggle="dropdown" href="#" id="navbarProjectDropdown" role="button">Project</a><div aria-labelledby="navbarProjectDropdown" class="dropdown-menu"><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Description">Description</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Design_Overview">Design Overview</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Background">Background</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Engineering">Engineering</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Contribution">Contribution</a></div></li><li class="nav-item dropdown"><a aria-expanded="false" aria-haspopup="true" class="nav-link dropdown-toggle" data-toggle="dropdown" href="#" id="navbarDryLabDropdown" role="button">DryLab</a><div aria-labelledby="navbarDryLabDropdown" class="dropdown-menu"><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Design">Design</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Model">Model</a></div></li><li class="nav-item dropdown"><a aria-expanded="false" aria-haspopup="true" class="nav-link dropdown-toggle" data-toggle="dropdown" href="#" id="navbarWetLabDropdown" role="button">WetLab</a><div aria-labelledby="navbarWetLabDropdown" class="dropdown-menu"><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Experiments">Experiments</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Results">Results</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Notebook">Notebook</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Protocols">Protocols</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Parts">Parts</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Safety">Safety</a></div></li><li class="nav-item dropdown"><a aria-expanded="false" aria-haspopup="true" class="nav-link dropdown-toggle" data-toggle="dropdown" href="#" id="navbarHuman PracticesDropdown" role="button">Human Practices</a><div aria-labelledby="navbarHuman PracticesDropdown" class="dropdown-menu"><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Human_Practices">Introduction</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Sustainability">Sustainability</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Implementation">Implementation</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Fighting_Underreporting">Fighting Underreporting</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Integrated_Human_Practices">Integrated Human Practices</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Education">Education</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Inclusivity">Inclusivity</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Communication">Communication</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Funding">Funding</a></div></li><li class="nav-item dropdown"><a aria-expanded="false" aria-haspopup="true" class="nav-link dropdown-toggle" data-toggle="dropdown" href="#" id="navbarTeamDropdown" role="button">Team</a><div aria-labelledby="navbarTeamDropdown" class="dropdown-menu"><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Team">Team Members</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Attributions">Attributions</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Collaborations">Collaborations</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Partnership">Partnership</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Sponsors">Sponsors</a></div></li><li class="nav-item dropdown"><a aria-expanded="false" aria-haspopup="true" class="nav-link dropdown-toggle" data-toggle="dropdown" href="#" id="navbarAwardsDropdown" role="button">Awards</a><div aria-labelledby="navbarAwardsDropdown" class="dropdown-menu"><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Medal_Criteria">Medal Criteria</a></div></li></ul></div></div></nav><header class="d-flex justify-content-center align-items-center"><div class="container"><h1>Parts</h1><p class="lead pl-1"></p><hr class="my-4"/></div></header><main><div class="container"><div class="row"><div class="sidebar col-lg-3"><div class="nav" id="contents"><h5>Contents</h5><ul></ul></div></div><div class="content col-lg-9"><article><h1>Overview</h1><p>Our designed parts from the four modules were classified into basic and composite parts, responsible for composing our strategy of paratransgenesis in the fight against leishmaniasis transmission. The basic parts are sequences designed during the development of our system and are used to form composite parts. Both classes of sequences can compose a Device that conducts an operation on the chassis. Also, we present the Kill the Leishmania Collection, a collection of our favorite parts that can be applied in approaches beyond paratransgenesis.</p><p>Although we could not test all parts, we provided detailed information or modeling of parts not evaluated in the Wet Lab. Due to COVID-19, we had difficulty accessing the laboratory, which made it impossible for us to evaluate all the drawn parts. However, we were able to assemble the parts BBa_K4075008 and BBa_K4075013, but transform only the part BBa_K4075008 into E. coli BL21(DE3) to evaluate the functionality of proDRS-N1.</p><h2>Part Collection</h2><p>This season our main focus was testing the Elimination Module, which motivated us to design a new strategy for modularizing the toxicity of AMPs. That's why we offer <strong>Kill the Leishmania Collection</strong>, a collection of parts that gathers AMPs and pro-peptides. These parts can drive other applications with different AMPs, as described by the Paratransgenesis Collection (from iGEM USP 2017) or Antimicrobial Peptides Collection (from iGEM NEU 2017)</p><h2>PARTS TABLE</h2><table><thead><tr><th>Name</th><th>Description</th><th>Designer</th><th>Class</th><th>Parts Collection</th></tr></thead><tbody><tr><td><a href="http://parts.igem.org/Part:BBa_K4075555">BBa_K4075555</a></td><td>Germination Measurement Device for B. subtilis</td><td>Samuel Chagas</td><td>Device</td><td></td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K4075001">BBa_K4075001</a></td><td>Natural Dermaseptin-H3 acidic pro-peptide</td><td>Giulio Braatz</td><td>Basic</td><td></td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K4075002">BBa_K4075002</a></td><td>Synthetic acidic pro-peptide</td><td>Júnior et al. [1]</td><td>Basic</td><td></td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K4075003">BBa_K4075003</a></td><td>Natural Dermaseptin-S1 acidic pro-peptide</td><td>Giulio Braatz</td><td>Basic</td><td></td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K4075004">BBa_K4075004</a></td><td>AMP DRS-N1</td><td>Giulio Braatz</td><td>Basic</td><td></td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K4075005">BBa_K4075005</a></td><td>AMP CAM-W</td><td>Giulio Braatz</td><td>Basic</td><td></td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K4075006">BBa_K4075006</a></td><td>AMP DRS-S1</td><td>Giulio Braatz</td><td>Basic</td><td></td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K4075007">BBa_K4075007</a></td><td>AMP DRS-H3</td><td>Giulio Braatz</td><td>Basic</td><td></td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K4075014">BBa_K4075014</a></td><td>proDRS-N1</td><td>Giulio Braatz</td><td>Composite</td><td>Collection</td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K4075015">BBa_K4075015</a></td><td>proCAM-W</td><td>Giulio Braatz</td><td>Composite</td><td>Collection</td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K4075016">BBa_K4075016</a></td><td>proDRS-S1</td><td>Giulio Braatz</td><td>Composite</td><td>Collection</td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K4075017">BBa_K4075017</a></td><td>proDRS-H3</td><td>Giulio Braatz</td><td>Composite</td><td>Collection</td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K4075018">BBa_K4075018</a></td><td>proE10+DRS-N1</td><td>Giulio Braatz</td><td>Composite</td><td>Collection</td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K4075009">BBa_K4075009</a></td><td>PT7_LacO+OmpA</td><td>Matheus Araujo</td><td>Composite</td><td></td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K4075008">BBa_K4075008</a></td><td>PT7_LacO+proDRS-N1+Term E. coli</td><td>Matheus Araujo</td><td>Device</td><td></td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K4075013">BBa_K4075013</a></td><td>PT7_LacO+mCherry+Term E. coli</td><td>Matheus Araujo</td><td>Device</td><td></td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K4075100">BBa_K4075100</a></td><td>C-Split Cas12a(Cpf1) with nMag for B. subtilis</td><td>Samuel Chagas</td><td>Basic</td><td></td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K4075100">BBa_K4075101</a></td><td>N-Split Cas12a(Cpf1) with pMag for B. subtilis</td><td>Samuel Chagas</td><td>Basic</td><td></td></tr></tbody></table></article></div></div></div></main><footer><div class="container" id="contfoot"><div id="div1"><p id="descri">A Project by</p><br/><img height="100px" src="https://static.igem.org/mediawiki/2021/d/dc/T--UNILA_LatAM--LogoV3.png" weight="100px"/><br/><br/><p id="text1">Contact</p><p>synfronteras@gmail.com</p></div><div id="div2"><p id="spon">Sponsor</p><br/><a href="https://www.itaipu.gov.br/"><img height="150px" id="spons" src="https://static.igem.org/mediawiki/2021/8/89/T--UNILA_LatAM--LogoIPH.png" weight="150px"/></a></div><div id="div3"><p id="fow">Follow us!</p><br/><img height="30px" id="aa" src="https://static.igem.org/mediawiki/2021/c/c9/T--UNILA_LatAM--fb.png" weight="30px"/><a href="https://www.facebook.com/synfronteras/">/synfronteras<br/></a><img height="30px" src="https://static.igem.org/mediawiki/2021/2/2c/T--UNILA_LatAM--ig.png" weight="30px"/><a href="https://www.instagram.com/igem_synfronteras/">@igem_synfronteras<br/></a><img height="30px" src="https://static.igem.org/mediawiki/2021/1/19/T--UNILA_LatAM--in.png" weight="30px"/><a href="https://br.linkedin.com/in/synfronteras-igem-team-77a7891ba">SynFronteras iGEM Team<br/></a><img height="30px" src="https://static.igem.org/mediawiki/2021/2/27/T--UNILA_LatAM--tw.png" weight="30px"/><a href="https://twitter.com/synfronteras">@synfronteras<br/></a><img height="30px" src="https://static.igem.org/mediawiki/2021/0/02/T--UNILA_LatAM--yt.png" weight="30px"/><a href="https://www.youtube.com/channel/UCHGAVNbPReEbzdEHeansZQg">SynFronteras-UNILA<br/></a></div></div></footer><script src="https://2021.igem.org/Template:UNILA_LatAm/content-bundleJS?action=raw&ctype=text/javascript"></script></body></html> |
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| − | <h1>Parts</h1>
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| − | <p>Each team will make new parts during iGEM and will add them to the Registry of Standard Biological Parts. iGEM provides an easy way to present the parts your team has created. The <code><groupparts></code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
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| − | <p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
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| − | <h3>Note</h3>
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| − | <p>Note that parts must be well documented on each Part's Main Page on the <a href="http://parts.igem.org/Main_Page">Registry</a>. This documentation includes all of the characterization data for your parts. <b>The part's data MUST be on the part's Main Page on the Registry for your team to be eligible for medals and special prizes pertaining to parts.</b> <br><br>
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| − | This page serves to <i>showcase</i> the parts you have made and should include links to the Registry pages for your parts. Future teams and other users are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
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| − | <h3>Adding parts to the Registry</h3>
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| − | <p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
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| − | <p>We encourage teams to start adding and documenting their parts on the Registry as soon as they can. Once you add your parts to the Registry, you can continue to add documentation to them throughout the iGEM season (up until the Registry freeze). This will allow you to remember all the details about your parts and store their history in the wiki. Documentation includes the characterization data of your parts.</p>
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| − | <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">
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| − | ADD PARTS
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| − | <h3>Inspiration: Basic Parts</h3>
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| − | <p>We have a created a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
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| − | <p> You can also take a look at the following examples for Basic Parts on the Registry:</p>
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| − | <li><a href="http://parts.igem.org/Part:BBa_K3114006">2019 Calgary </a></li>
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| − | <li><a href="http://parts.igem.org/Part:BBa_K3027000">2019 GO Paris Saclay</a></li>
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| − | <li><a href="http://parts.igem.org/Part:BBa_K3187028">2019 TU Darmstadt</a></li>
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| − | <li><a href="http://parts.igem.org/Part:BBa_K3552000">2020 Links China</a></li>
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| − | <li><a href="http://parts.igem.org/Part:BBa_K3558000">2020 UNSW Australia</a></li>
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| − | <li><a href="http://parts.igem.org/Part:BBa_K3338002">2020 Hannover</a></li>
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| − | <h3>Inspiration: Composite Parts</h3>
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| − | <p>We have a created a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
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| − | <p> You can also take a look at the following examples for Composite Parts on the Registry:</p>
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| − | <li><a href="http://parts.igem.org/Part:BBa_K3198007">2019 NUS Singapore </a></li>
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| − | <li><a href="http://parts.igem.org/Part:BBa_K2932003">2019 Mingdao</a></li>
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| − | <li><a href="http://parts.igem.org/Part:BBa_K2980009">2019 Tsinghua</a></li>
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| − | <li><a href="http://parts.igem.org/Part:BBa_K3407022">2020 TUDelft</a></li>
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| − | <li><a href="http://parts.igem.org/Part:BBa_K3380500">2020 Edinburgh</a></li>
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| − | <li><a href="http://parts.igem.org/Part:BBa_K3512042">2020 BITSPilani Goa India</a></li>
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| − | <h3>What information do I need to start putting my parts on the Registry?</h3>
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| − | <p>The information needed to initially create a part on the Registry is:</p>
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| − | <li>Part Name</li>
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| − | <li>Part type</li>
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| − | <li>Creator</li>
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| − | <li>Sequence</li>
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| − | <li>Short Description (60 characters on what the DNA does)</li>
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| − | <li>Long Description (Longer description of what the DNA does)</li>
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| − | <li>Design considerations</li>
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| − | We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, you must also put it up on the part page. </p>
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| − | <p>Please include a table of all the parts your team has made during your project on this page. Remember part characterization and measurement data must go on your team part pages on the Registry. </p>
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| − | <groupparts>iGEM20 UNILA_LatAm</groupparts>
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