| − | <!-- # TODO: #6 Fix table caption font--><!-- # TODO: #7 Fix citations links font size--><html lang="en"><head><meta charset="utf-8"/><meta content="width=device-width,initial-scale=1" name="viewport"/><title>Protocols | iGEM UNILA_LatAm</title><script src="https://2020.igem.org/common/MathJax-2.5-latest/MathJax.js?config=TeX-AMS-MML_HTMLorMML"></script><link href="https://2021.igem.org/Template:UNILA_LatAm/css/contentCSS?action=raw&ctype=text/css" rel="stylesheet"/></head><body><!-- # TODO: #6 Fix table caption font--><!-- # TODO: #7 Fix citations links font size--><nav class="navbar navbar-expand-xl fixed-top"><div class="container d-flex justify-content-between"><a class="navbar-brand d-lg-inline-block" href="https://2021.igem.org/Team:UNILA_LatAm"><img ;="" src="https://static.igem.org/mediawiki/2021/9/98/T--UNILA_LatAm--Logo.png" style="width:auto; height:80px ;position: relative; margin-top:15px"/></a><button aria-controls="navbarNav" aria-expanded="false" 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href="https://2021.igem.org/Team:UNILA_LatAm/Collaborations">Collaborations</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Sponsors">Sponsors</a></div></li><li class="nav-item dropdown"><a aria-expanded="false" aria-haspopup="true" class="nav-link dropdown-toggle" data-toggle="dropdown" href="#" id="navbarAwardsDropdown" role="button">Awards</a><div aria-labelledby="navbarAwardsDropdown" class="dropdown-menu"><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Medal_Criteria">Medal Criteria</a><a class="dropdown-item" href="https://2021.igem.org/Team:UNILA_LatAm/Judging_Form">Judging Form</a></div></li></ul></div></div></nav><header class="d-flex justify-content-center align-items-center"><div class="container"><h1>Protocols</h1><p class="lead pl-1">asdasd.</p><hr class="my-4"/></div></header><main><div class="container"><div class="row"><div class="sidebar col-lg-3"><div class="nav" id="contents"><h5>Contents</h5><ul></ul></div></div><div class="content col-lg-9"><article><h1>Headings</h1><h2>Level 2 Heading</h2><h3>Level 3 Heading</h3><h4>Level 4 Heading</h4><h1>Emphasis</h1><p>This is regular text.</p><p><strong>This is bold text.</strong> <strong>This is also bold text.</strong></p><p><em>This is italic text.</em> _This is also italix text.*</p><p><em><strong>This text is bold and italic both.</strong></em> <em><strong>This text is bold and italic both.</strong></em></p><p><s>Strikethrough</s></p><h1>Lists</h1><ol><li>This is item one.</li><li>This is item two.</li><li>It's okay to number every item as 1.<ul><li>This is a nested list.</li><li>Use asterisk for an unordered list,</li><li>Further nesting?</li></ul><ul><li>Plus sign also works.</li></ul><ul><li>And so does minus.</li></ul></li><li><s>Birds aren't real.</s></li></ol><h1>Links</h1><p><a href="https://google.com">Google</a></p><p><a href="https://2021.igem.org/Team:UNILA_LatAm/Protocols/Link-goes-in-parenthesis">Text goes in square brackets</a></p><h1>Blockquote</h1><blockquote><p>This is a blockquote. This continues in the same paragraph.</p><p>To change the paragraph, you have to leave a line.</p></blockquote><blockquote><p>This is another blockquote.</p><p><strong>Pranav Ballaney, 2020</strong></p></blockquote><h1>Images</h1><p>This is a regular paragraph.</p><h4>This is a level 4 heading.</h4><p><a href="google.com">This is a link</a></p><div class="image"><img alt="Caption" src="https://static.igem.org/mediawiki/2021/3/3f/T--UNILA_LatAm--img--Description--josh-withers.jpg" style="width: 100%"/><p>Figure 1: Caption</p></div><p>Back to regular text.</p><h1>Tables</h1><p>This is a regular paragraph, which precedes the table. When you want to insert a table, indent one level back and specify the plugin. Then indent inside again and start writing the table.</p><table><caption id="table1captiongoeshere">Table 1: Caption goes here.</caption><thead><tr><th>This is</th><th>the table</th><th>header row</th></tr></thead><tbody><tr><td>1</td><td>2</td><td>3</td></tr><tr><td>4</td><td>5</td><td>6</td></tr></tbody></table><p>And when you're done, go back to the regular markdown filter.</p><p><strong>Example 2:</strong></p><table><thead><tr><th style="text-align:left">Left aligned</th><th style="text-align:center">Center aligned</th><th style="text-align:right">Right aligned</th></tr></thead><tbody><tr><td style="text-align:left">1</td><td style="text-align:center">2</td><td style="text-align:right">3</td></tr><tr><td style="text-align:left">4</td><td style="text-align:center">5</td><td style="text-align:right">6</td></tr></tbody></table><p><strong>Example 3:</strong></p><table><caption id="table3captiongoeshere">Table 3: Caption goes here.</caption><thead><tr><th></th><th colspan="2" style="text-align:center">Grouping</th></tr><tr><th>First Header</th><th style="text-align:center">Second Header</th><th style="text-align:right">Third Header</th></tr></thead><tbody><tr><td>Content</td><td colspan="2" style="text-align:center"><em>Long Cell</em></td></tr><tr><td>Content</td><td style="text-align:center"><strong>Cell</strong></td><td style="text-align:right">Cell</td></tr><tr><td>New section</td><td style="text-align:center">More</td><td style="text-align:right"><a href="https://google.com">Data</a></td></tr><tr><td>And more</td><td colspan="2" style="text-align:center">With an escaped '|'</td></tr></tbody></table><h1>Definitions</h1><p><dfn>Term ~ Definition</dfn></p><p>This can come <dfn>anywhere in ~ the text</dfn>.</p><h1>MathJax</h1><p>$$\int_{0}^{\infty} e^{-x^2} dx = \frac{\sqrt{\pi}}{2}$$</p><p>$$-\frac{\hbar^2}{2m}\nabla^2\psi + V\psi = E\psi$$</p><p>$$i\hbar\gamma^\mu\partial_\mu\psi = mc\psi$$</p><p>$$y_k = \frac{1}{\sqrt{N}}\sum_{n=0}^{N-1}x_n\omega_N^{kn}$$</p><h1>Citations</h1><p>In text citation for a research article with a DOI. <a href="#citation2">Rosano et al., 2019</a></p><p>In text citation for another research article with a DOI. <a href="#citation1">Allen & Sheridan, 2015</a></p><p>In text citation for a book with no DOI. <a href="#citation3">Ingalls, 2013</a></p><p>In text citation for a website with institutional author. <a href="#citation4">TNAU Agritech Portal, n.d.</a></p><p>In text citation for a website with an author. <a href="#citation5">Author, n.d.</a></p></article><article id="references"><h1>References</h1><ol><li id="citation1"><p class="author">Allen, M. J., & Sheridan, S. C. (2015).</p><cite>Mortality risks during extreme temperature events (ETEs) using a distributed lag non-linear model.</cite><p><span class="journalTitle">International Journal of Biometeorology</span> <span class="journalInfo">62(1), 57-67.</span></p><a class="in-text" href="https://doi.org/10.1007/s00484-015-1117-4" rel="noopener" target="_blank">CrossRef</a><a class="in-text" href="https://scholar.google.com/scholar?q=Mortality risks during extreme temperature events (ETEs) using a distributed lag non-linear model." rel="noopener" target="_blank">Google Scholar</a><a class="in-text" href="#intext1">Back to text</a></li><li id="citation2"><p class="author">Rosano, A., Bella, A., Gesualdo, F., Acampora, A., Pezzotti, P., Marchetti, S., ... & Rizzo, C. (2019).</p><cite>Investigating the impact of influenza on excess mortality in all ages in Italy during recent seasons (2013/14-2016/17 seasons).</cite><p><span class="journalTitle">International Journal of Infectious Diseases</span> <span class="journalInfo">88, 127-134.</span></p><a class="in-text" href="https://doi.org/10.1016/j.ijid.2019.08.003" rel="noopener" target="_blank">CrossRef</a><a class="in-text" href="https://scholar.google.com/scholar?q=Investigating the impact of influenza on excess mortality in all ages in Italy during recent seasons (2013/14-2016/17 seasons)." rel="noopener" target="_blank">Google Scholar</a><a class="in-text" href="#intext2">Back to text</a></li><li id="citation3"><p class="author">Ingalls, B. P. (2013).</p><cite>Mathematical modeling in systems biology: An introduction.</cite><p><span class="details">MIT Press.</span></p><a class="in-text" href="https://books.google.co.in/books?id=OYr6AQAAQBAJ" rel="noopener" target="_blank">Google Books</a><a class="in-text" href="#intext3">Back to text</a></li><li id="citation4"><p class="author"></p><cite>Agriculture: Crop production: Sugarcane. TNAU Agritech Portal.</cite><p><span class="details">(March 15, 2019). Retrieved on June 22, 2020. from </span><a class="in-text" href="https://google.com" rel="noopener" target="_blank">https://google.com</a></p><a class="in-text" href="#intext4">Back to text</a></li><li id="citation5"><p class="author">Author Name. (n.d.).</p><cite>Agriculture: Crop production: Sugarcane. TNAU Agritech Portal.</cite><p><span class="details">Retrieved on June 22, 2020. from </span><a class="in-text" href="https://google.com" rel="noopener" target="_blank">https://google.com</a></p><a class="in-text" href="#intext5">Back to text</a></li></ol></article></div></div></div></main><footer><div class="container" id="contfoot"><div id="div1"><br/><p>BioPank is a project developed by</p><p>the Syn Fronteras team</p><br/><br/><br/><p id="text1">Contact</p><p>synfronteras@gmail.com</p></div><div id="div2"><p id="fow">Follow us!</p><div id="teste"><img src="https://static.igem.org/mediawiki/2021/c/c9/T--UNILA_LatAM--fb.png"/></div><a href="https://www.facebook.com/synfronteras/">/synfronteras<br/></a><img src="https://static.igem.org/mediawiki/2021/2/2c/T--UNILA_LatAM--ig.png"/><a href="https://www.instagram.com/igem_synfronteras/">@igem_synfronteras<br/></a><img src="https://static.igem.org/mediawiki/2021/1/19/T--UNILA_LatAM--in.png"/><a href="https://br.linkedin.com/in/synfronteras-igem-team-77a7891ba">SynFronteras iGEM Team<br/></a><img src="https://static.igem.org/mediawiki/2021/2/27/T--UNILA_LatAM--tw.png"/><a href="https://twitter.com/synfronteras">@synfronteras<br/></a><img src="https://static.igem.org/mediawiki/2021/0/02/T--UNILA_LatAM--yt.png"/><a href="https://www.youtube.com/channel/UCHGAVNbPReEbzdEHeansZQg">SynFronteras-UNILA<br/></a></div></div></footer><script src="https://2021.igem.org/Template:UNILA_LatAm/content-bundleJS?action=raw&ctype=text/javascript"></script></body></html>
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class="sidebar col-lg-3"><div class="nav" id="contents"><h5>Contents</h5><ul></ul></div></div><div class="content col-lg-9"><article><h2>The protocols SynFronteras Team used for lab experiments.</h2><div><iframe height="800px" src="https://static.igem.org/mediawiki/2021/b/be/T--UNILA_LatAM--Protocols.pdf" width="100%"></iframe></div></article></div></div></div></main><footer><div class="container" id="contfoot"><div id="div1"><p id="descri">A Project by</p><br/><img height="100px" src="https://static.igem.org/mediawiki/2021/d/dc/T--UNILA_LatAM--LogoV3.png" weight="100px"/><br/><br/><p id="text1">Contact</p><p>synfronteras@gmail.com</p></div><div id="div2"><p id="spon">Sponsor</p><br/><a href="https://www.itaipu.gov.br/"><img height="150px" id="spons" src="https://static.igem.org/mediawiki/2021/8/89/T--UNILA_LatAM--LogoIPH.png" weight="150px"/></a></div><div id="div3"><p id="fow">Follow us!</p><br/><img height="30px" id="aa" 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