Difference between revisions of "Team:CKWA-China/Attributions"

 
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                 <h1>Content</h1>
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                 <h1>Attributions</h1>
 
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                     <li><a href="https://2021.igem.org/Team:CKWA-China">Home</a></li>
 
                     <li><a href="https://2021.igem.org/Team:CKWA-China">Home</a></li>
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                     <li>Content</li>
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                     <li>Attributions</li>
 
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                         <h2 class="point" id="point-1">Literature Review</h2>
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                         <h2 class="point" align="center"id="point-1">Attributions</h2>
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                         <p>As we all know, potato (Solanum tuberosum) is a globally important high-yield crop that produces nutrient-rich tubers. This non-grain crop is the third most important food crop, after wheat and rice (Patil et al.2017). One of the major threats to potato production is soft rot disease caused by Erwinia bacteria, which generally occur during cultivation, harvesting or transportation and storage of farm produce, resulting in considerable yield reduction, poor quality of produce, and economic loss. In Kenya, it causes up to 50% total crop loss (Onkendi and Moleleki 2014; Muturi et al.2018).</p>
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               <th> </th>
 
               <th> </th>
 
               <th>Project Design</th>
 
               <th>Project Design</th>
 
               <th>Human Practice</th>
 
               <th>Human Practice</th>
 
               <th>Lab</th>
 
               <th>Lab</th>
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               <th>Model</th>
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               <th>Art Design</th>
 
               <th>Art Design</th>
 
               <th>Wiki Construction</th>
 
               <th>Wiki Construction</th>
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               <th> BP writing </th>
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             <tr>
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               <td style="background-color: rgb(118,191,141);"> Linda Liu </td>
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               <td style="background-color: rgb(118, 179, 191);"> Meiyi Li</td>
 
               <td>✔</td>
 
               <td>✔</td>
 
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             <tr>
 
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               <td style="background-color: rgb(118,191,141);"> Hedy Zhu </td>
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               <td style="background-color: rgb(118, 179, 191);">Haoyu Wang</td>
 
               <td>✔</td>
 
               <td>✔</td>
 
               <td>✔✔</td>
 
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               <td></td>
 
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             </tr>
 
             </tr>
 
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               <td style="background-color: rgb(118,191,141);"> Harry Fang </td>
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               <td style="background-color: rgb(118, 179, 191);"> Yuxuan Shen</td>
 
               <td>✔✔</td>
 
               <td>✔✔</td>
 
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               <td style="background-color: rgb(118,191,141);"> Annie Xian </td>
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               <td style="background-color: rgb(118, 179, 191);"> Jiazhen Ren </td>
 
               <td>✔</td>
 
               <td>✔</td>
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               <td>✔✔</td>
 
               <td>✔</td>
 
               <td>✔</td>
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              <td></td>
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              <td></td>
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             <td>✔</td>
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             </tr>
 
             </tr>
 
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               <td style="background-color: rgb(118,191,141);"> Jiayi Li </td>
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               <td style="background-color: rgb(118, 179, 191);"> Chang Yang</td>
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               <td></td>
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               <td>✔</td>
 
               <td>✔✔</td>
 
               <td>✔✔</td>
 
               <td>✔</td>
 
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               <td style="background-color: rgb(118,191,141);"> Nick Chen </td>
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               <td style="background-color: rgb(118, 179, 191);"> WU Taibo </td>
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               <td></td>
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               <td>✔</td>
 
               <td>✔</td>
 
               <td>✔</td>
 
               <td></td>
 
               <td></td>
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              <td></td>
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               <td>✔✔</td>
 
               <td>✔✔</td>
 
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               <td style="background-color: rgb(118,191,141);"> David Wei </td>
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               <td style="background-color: rgb(118, 179, 191);"> Alfred Jan Wang </td>
 
               <td>✔</td>
 
               <td>✔</td>
 
               <td>✔</td>
 
               <td>✔</td>
 
               <td></td>
 
               <td></td>
 
               <td>✔✔</td>
 
               <td>✔✔</td>
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              <td>✔</td>
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             </tr>
 
             </tr>
 
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               <td style="background-color: rgb(118,191,141);"> Danny Cui </td>
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               <td style="background-color: rgb(118, 179, 191);"> Kevin Deng</td>
 
               <td>✔</td>
 
               <td>✔</td>
 
               <td>✔</td>
 
               <td>✔</td>
 
               <td>✔</td>
 
               <td>✔</td>
 
               <td></td>
 
               <td></td>
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              <td></td>
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             <td>✔</td>
 
             <td>✔</td>
 
             </tr>
 
             </tr>
 
  <tr>
 
  <tr>
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               <td style="background-color: rgb(118,191,141);"> Ian Liu </td>
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               <td style="background-color: rgb(118, 179, 191);"> Yuehao Wang </td>
 
               <td>✔</td>
 
               <td>✔</td>
 
               <td>✔</td>
 
               <td>✔</td>
 
               <td>✔</td>
 
               <td>✔</td>
 
               <td></td>
 
               <td></td>
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              <td></td>
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              <td></td>
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             <td>✔✔</td>
 
             <td>✔✔</td>
 
             </tr>
 
             </tr>
 
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               <td style="background-color: rgb(118,191,141);"> Holly Zhuang </td>
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               <td style="background-color: rgb(118, 179, 191);"> DingFeng Zhang </td>
 
               <td>✔</td>
 
               <td>✔</td>
 
               <td>✔</td>
 
               <td>✔</td>
 
               <td>✔</td>
 
               <td>✔</td>
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              <td></td>
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              <td>✔</td>
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               <td>✔</td>
 
               <td>✔</td>
 
             <td>✔</td>
 
             <td>✔</td>
 
             </tr>
 
             </tr>
 
  <tr>
 
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               <td style="background-color: rgb(118,191,141);"> Zoe Li </td>
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               <td style="background-color: rgb(118, 179, 191);">Junfeng Lin </td>
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              <td></td>
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               <td>✔</td>
 
               <td>✔</td>
 
               <td>✔</td>
 
               <td>✔</td>
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              <td></td>
 
 
               <td>✔</td>
 
               <td>✔</td>
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               <td>✔</td>
 
               <td>✔</td>
 
             <td>✔</td>
 
             <td>✔</td>
 
             </tr>
 
             </tr>
 
  <tr>
 
  <tr>
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               <td style="background-color: rgb(118,191,141);"> Peter Qi </td>
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               <td style="background-color: rgb(118, 179, 191);"> Yunbo Zhao </td>
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              <td>✔✔</td>
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              <td></td>
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              <td>✔✔</td>
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              <td></td>
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              <td></td>
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              <td></td>
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              <td style="background-color: rgb(118,191,141);"> Richard Han </td>
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              <td>✔</td>
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               <td>✔</td>
 
               <td>✔</td>
 
               <td></td>
 
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              <td></td>
 
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            <td></td>
 
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            </tr>
 
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<tr>
 
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              <td style="background-color: rgb(118,191,141);"> Rouxuan Yu </td>
 
 
               <td>✔</td>
 
               <td>✔</td>
 
               <td></td>
 
               <td></td>
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              <td>✔</td>
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              <td></td>
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              <td></td>
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              <td></td>
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              <td style="background-color: rgb(118,191,141);"> Oliver Yuan </td>
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              <td></td>
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              <td></td>
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              <td>✔</td>
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              <td></td>
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              <td></td>
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            <td></td>
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              <td style="background-color: rgb(118,191,141);"> Sam Wang </td>
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              <td>✔</td>
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              <td></td>
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              <td>✔</td>
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             <td></td>
 
             <td></td>
 
             </tr>
 
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                             <h3 class="point" id="point-2">Project Design</h3>
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                                        <p>We then recognized an absence in both detection and treatment of Soft Rot Disease. Therefore, we understood the importance of a bio-control solution that is not only effective but also environmentally-friendly. Based on knowledge of the field of synthetic biology, we started to come up with different approaches to soft rot detection and treatment.</p>
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                                        <div class="image"><a href="" class="lightbox-image"><img src="https://static.igem.org/mediawiki/2020/c/cb/T--iBowu-China--keystone1.png" alt=""></a>
 
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                            <h3 class="point" id="point-2">Interview（Potato Center Asia-Pacific Headquarter）</h3>
 
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                            <p>We met with senior staffs of the potato center Asia-Pacific Headquarter to learn about the current detection and treatments of potato diseases. We then visited their workplace, which accounted for a large proportion of the current potato-agricultural development. We presented our initial concept to the experts (including the chairman of the potato center, Mr. Lu) and audited a group meeting on agricultural pharmacology. Through the discussions with them, we became more aware of the consequences of Soft Rot disease and the importance of creating an effective bio-control solution.</p>
 
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                            <!-- 视频排版 -->
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The initial experience of the project was inspired by our collaborative team iBowu-China's cancer project last year and by the inspiration given to the group in a literature. All team members were involved in this work.</p>
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                            <h3 class="point" id="point-3">Experts</h3>
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<h3 class="point" id="point-2">LAB</h3>
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                            <p>Our team had the pleasure of meeting with Dr. Flemming Bensenbacher on 5th July 2019. Dr. Bensenbacer is an academician of the Royal Danish academy of Sciences and President of Carlsberg Foundation. He shared his journey in scientific research with us and the problems he encountered and tackled. We presented our project to him and hosted an interview with questions relating to the application of synthetic biology. This opportunity was crucial to us as we gained from it a valuable depth of knowledge in the potential future pathways of our product development.</p>
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                            <p>
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<p>Plasmid construction:Meiyi Li, Haoyu Wang, Chang Yang</br>
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                                <video style="width: 100%;" src="video/mda-kc21c5bv2fig3ieu.mp4" controls="“ture”"></video>
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Protein Extraction:Meiyi Li, Haoyu Wang , Kevin Deng, Yuehao Wang, DingFeng Zhnag, Junfeng Lin, Yunbo Zhao</br></p>
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                    <div class="row clearfix">
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                        <div class="image-column col-md-5 col-sm-12 col-xs-12">
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<h3 class="point" id="point-2">SDS-PAGE</h3>
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                            <div class="image"><a href="" class="lightbox-image"><img
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<p>The SDS-PAGE technique was supervised by Prof. Li Lina from Beijing University of Traditional Chinese Medicine.</p>
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                                        src="https://static.igem.org/mediawiki/2020/c/cb/T--iBowu-China--keystone1.png" alt=""></a></div>
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                        </div>
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<h3 class="point" id="point-2">Human Practice</h3>
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                        <div class="info-column col-md-7 col-sm-12 col-xs-12">
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<p>
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                            <div class="inner-column">
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Literature Review:Meiyi Li，Haoyu Wang，Chang Yang，WU Taibo，Yuxuan Shen，Jiazhen Ren</p>
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                                <h2>Project Analysis</h2>
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<p>
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                                <span class="sub-title">We Reserch deeply to give the best quality services.</span>
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Interview：Kevin Deng，Meiyi Li，Jiazhen Ren，Junfeng Lin</p>
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                                <h5>Here our project analysis result.</h5>
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<p>
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                                <ul class="list-style-one">
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School lecture：Meiyi Li，Haoyu Wang，Chang Yang，WU Taibo，Jiazhen Ren</p>
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                                    <li>It was popularised in the with the release of sheets containing Lorem Ipsum</li>
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<h3 class="point" id="point-2">Art Design</h3>
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                                    <li>Page Maker including versions of Lorem Ipsum.</li>
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                                    <li>It is a long established fact that a reader will be distracted by the readable
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<p>
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                                    </li>
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Yuxuan Shen,WU Taibo,Alfred Jan Wang,DingFeng Zhang,Junfeng Lin</p>
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                                    <li>content of a page when looking at its layout. The point of using Lorem Ipsum
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<h3 class="point" id="point-2">Wiki Construction</h3>
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                                    </li>
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                                    <li>is that it has ult model text, and a search for 'lorem ipsum' will uncover many
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<p>
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                                        web</li>
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Meiyi Li，Yuehao Wang,Kevin Deng,DingFeng Zhang,Junfeng Lin</p>
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                                </ul>
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<h3 class="point" id="point-2">Plasmid synthesis </h3>
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                            </div>
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                        </div>
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<p>Our initial plasmid was synthesized with the help of BGI, who also gave us expression optimization of our plasmid profile, thanks to them.</p>
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                    </div>
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<h3 class="point" id="point-2">Experimental guidance</h3>
 +
 
 +
<p>We are grateful to Prof. Li Lina of Beijing University of Traditional Chinese Medicine for providing us with guidance on laboratory operations.</p>
 +
    <h3 class="point" id="point-2">Protein Purification </h3>
 +
 
 +
<p>We would also like to give special thanks to the BGI-write Corporation company that performed the protein purification experiments for us. Since our lab does not have protein purification equipment, we commissioned BGI-write Corporation  to help us with our protein purification work.
 +
  </p>
 +
    <h3 class="point" id="point-2">Promotional Video    </h3>
 +
  <p>We would also like to thank the professional camera team, the director and the post-editors who helped us shoot the film.</br>
 +
Director Chang Liu </br>
 +
Photographer Hongbo Lu </br>
 +
Video Editing Hongfu Wang </br>
 +
Subtitles Mohan Zhang  </br>
 +
</p>        
 +
                         
 +
<h3 class="point" id="point-2">Acknowledgment</h3>
 +
 
 +
<p>
 +
Throughout the 11 months of preparation for iGEM, we encountered many difficulties and hardships, among which we would like to especially thank the iBowu-China team members, as well as several members of the CCIC, for providing us with a lot of help.
 +
 
 +
 
 +
</p>
 +
                       
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             </div>
 
         </section>
 
         </section>

Latest revision as of 10:49, 21 October 2021

Attributions

Attributions

Project Design Human Practice Lab Art Design Wiki Construction
Meiyi Li ✔ ✔✔ ✔ ✔
Haoyu Wang ✔ ✔✔ ✔
Yuxuan Shen ✔✔ ✔✔ ✔
Jiazhen Ren ✔ ✔✔ ✔ ✔ ✔
Chang Yang ✔ ✔✔ ✔
WU Taibo ✔ ✔ ✔✔
Alfred Jan Wang ✔ ✔ ✔✔
Kevin Deng ✔ ✔ ✔ ✔
Yuehao Wang ✔ ✔ ✔ ✔✔
DingFeng Zhang ✔ ✔ ✔ ✔ ✔
Junfeng Lin ✔ ✔ ✔ ✔ ✔
Yunbo Zhao ✔ ✔

Project Design

The initial experience of the project was inspired by our collaborative team iBowu-China's cancer project last year and by the inspiration given to the group in a literature. All team members were involved in this work.

LAB

Plasmid construction:Meiyi Li, Haoyu Wang, Chang Yang
Protein Extraction:Meiyi Li, Haoyu Wang , Kevin Deng, Yuehao Wang, DingFeng Zhnag, Junfeng Lin, Yunbo Zhao

SDS-PAGE

The SDS-PAGE technique was supervised by Prof. Li Lina from Beijing University of Traditional Chinese Medicine.

Human Practice

Literature Review:Meiyi Li，Haoyu Wang，Chang Yang，WU Taibo，Yuxuan Shen，Jiazhen Ren

Interview：Kevin Deng，Meiyi Li，Jiazhen Ren，Junfeng Lin

School lecture：Meiyi Li，Haoyu Wang，Chang Yang，WU Taibo，Jiazhen Ren

Art Design

Yuxuan Shen,WU Taibo,Alfred Jan Wang,DingFeng Zhang,Junfeng Lin

Wiki Construction

Meiyi Li，Yuehao Wang,Kevin Deng,DingFeng Zhang,Junfeng Lin

Plasmid synthesis

Our initial plasmid was synthesized with the help of BGI, who also gave us expression optimization of our plasmid profile, thanks to them.

Experimental guidance

We are grateful to Prof. Li Lina of Beijing University of Traditional Chinese Medicine for providing us with guidance on laboratory operations.

Protein Purification

We would also like to give special thanks to the BGI-write Corporation company that performed the protein purification experiments for us. Since our lab does not have protein purification equipment, we commissioned BGI-write Corporation to help us with our protein purification work.

Promotional Video

We would also like to thank the professional camera team, the director and the post-editors who helped us shoot the film.
Director Chang Liu
Photographer Hongbo Lu
Video Editing Hongfu Wang
Subtitles Mohan Zhang

Acknowledgment

Throughout the 11 months of preparation for iGEM, we encountered many difficulties and hardships, among which we would like to especially thank the iBowu-China team members, as well as several members of the CCIC, for providing us with a lot of help.