Difference between revisions of "Team:CKWA-China/Description"

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    <section class="page-title" style="background-image:url(https://static.igem.org/mediawiki/2021/8/84/T--CKWA-China--Desc1.jpeg);">
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        <div class="auto-container">
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            <div class="inner-container clearfix">
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                <h1>Description</h1>
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                <ul class="bread-crumb clearfix">
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                    <li><a href="https://2021.igem.org/Team:CKWA-China">Home</a></li>
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                    <li>Description</li>
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                </ul>
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            </div>
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        </div>
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    </section>
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    <!-- Service Detail Section -->
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<h3>★  ALERT! </h3>
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    <div class="sidebar-page-container alternate">
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<p>This page is used by the judges to evaluate your team for the <a href="https://2021.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2021.igem.org/Judging/Awards"> award listed below</a>. </p>
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        <div class="auto-container">
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2021.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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                        <div class="lower-content">
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                            <h2 class="point" id="point-1">Description</h2>
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<h3 class="point" id="point-11">1. A major problem in cancer.</h3>
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                            <p>
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Recurrence of cancer after treatment is a major problem facing humans today, and cancer recurrence is a great threat for cancer patients. Among them, overexpression of c-myc protein is closely related to this process, and patients with overactivation of c-myc gene in cancer cells tend to have poor prognosis.</p>
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<h3 class="point" id="point-2">1.1 What are myc genes?</h3>
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                            <p>
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myc genes have been shown to be a class of regulatory genes that are transcription factors that can encode myc-like proteins to become transcription factors transcription factors. they are essential for cell growth, metabolism and tissue development.</p>
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<h3 class="point" id="point-12">1.2 Impact of myc in cancer</h3>
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<p>
  
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<div class="clear"></div>
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They are essential for cell growth, metabolism and tissue development. Also, MYC is an important group of oncogenes. c-MYC is aberrantly expressed in about 30-50% of human malignancies and plays an extremely important role in affecting cell growth, proliferation, differentiation, apoptosis, as well as cell metabolism and malignant transformation. aberrantly high expression of c-MYC is associated with poor prognosis in many human cancers.
  
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<div class="column full_size">
 
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<h1>Project Description </h1>
 
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<h3>Bronze Medal Criterion #3</h3>
 
  
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<p>Describe how and why you chose your iGEM project.
 
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<br><br>
 
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Please see the <a href="https://2021.igem.org/Judging/Medals">2021 Medals Page</a> for more information.
 
 
</p>
 
</p>
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</div>
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<h3 class="point" id="point-13">1.3 C-myc genes</h3>
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<p>
  
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The Myc family consists of three related human genes: </br>
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- c-myc (MYC), the first gene to be discovered in this family </br>
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- l-myc (MYCL) </br>
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- n-myc (MYCN) </br>
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</p>
  
  
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<div class="column two_thirds_size">
 
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<h3>What should this page contain?</h3>
 
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<ul>
 
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<li> A clear and concise description of your project.</li>
 
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<li>A detailed explanation of why your team chose to work on this particular project.</li>
 
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<li>References and sources to document your research.</li>
 
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<li>Use illustrations and other visual resources to explain your project.</li>
 
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</ul>
 
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</div>
 
  
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<div class="column third_size" >
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<h3 class="point" id="point-2">2. New discoveries.</h3>
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<div class="highlight decoration_A_full">
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                            <p>
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<h3>Inspiration</h3>
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<p>See how other teams have described and presented their projects: </p>
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<ul>
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<li><a href="https://2019.igem.org/Team:Leiden/Description">2019 Leiden</a></li>
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<li><a href="https://2019.igem.org/Team:ITESO_Guadalajara/Description">2019 ITESO Guadalajara</a></li>
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<li><a href="https://2020.igem.org/Team:Technion-Israel/Description">2020 Technion Israel</a></li>
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<li><a href="https://2020.igem.org/Team:Botchan_Lab_Tokyo/Description">2020 Botchan Lab Tokyo</a></li>
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<li><a href="https://2020.igem.org/Team:St_Andrews/Description">2020 St Andrews</a></li>
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<li><a href="https://2020.igem.org/Team:MIT/Description">2020 MIT</a></li>
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</ul>
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</div>
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</div>
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<div class="column two_thirds_size" >
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<h3>Advice on writing your Project Description</h3>
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Scientists have recently discovered that a Lon protease from bacteria can effectively degrade c-myc protein, and this may be one of the reasons why bladder cancer patients with urinary tract infection with this bacteria have a better prognosis instead. Thus, Lon protein is expected to be a new macromolecular drug in the fight against cancer in humans.</p>
 +
<h3 class="point" id="point-21">2.1 Discovery of c-myc protein inhibition</h3>
 
<p>
 
<p>
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We encourage you to put up a lot of information and content on your wiki, but we also encourage you to include summaries as much as possible. If you think of the sections in your project description as the sections in a publication, you should try to be concise, accurate, and unambiguous in your achievements. Your Project Description should include more information than your project abstract.
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MYC is important for the growth and development of the kidney. It has been shown that severe kidney infections impair kidney growth in childhood and that bladder cancer in patients with urinary tract infections instead has a better prognosis.
 +
Inference: MYC levels in the kidney may be disturbed by bacterial infections.
 
</p>
 
</p>
 +
<h3 class="point" id="point-22">2.2 Bacterial proteases deplete c-MYC and increase survival in mouse models of bladder and colon cancer</h3>
 +
<p>
 +
Uropathogenic Escherichia coli (UPEC) can degrade c-MYC protein and attenuate MYC gene expression in human cells and animal tissues. Also, c-MYC protein can be degraded by cell-free Uropathogenic Escherichia coli (UPEC) lysates, as well as rapidly by purified Lon protease.
 +
</p>
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<h3 class="point" id="point-2">2.3 Literature conclusions</h3>
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<p>
  
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</div>
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Lon protease from uropathogenic Escherichia coli (UPEC) is able to degrade c-MYC protein and attenuate MYC gene expression in human cells and animal tissues.
 +
Experiments in mouse models have shown that Lon protease delivered either intravesically or orally can delay tumor progression and improve survival in both MYC-dependent bladder and colon cancer models.
 +
</p>
  
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<div class="column third_size">
 
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<h3>References</h3>
 
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<p>iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you thought about your project and what works inspired you.</p>
 
  
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</div>
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<h3 class="point" id="point-3">3. Our solution</h3>
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                            <p>
  
  
 +
We try to express Lon protease in various systems and test the yield of Lon protease in different environments. After achieving stable laboratory production, we will conduct in vitro cellular experiments to verify the inhibition ability of Lon protease on c-myc, and then we will try to optimize Lon protease from protein structure, find its effective gene fragment, adopt mRNA delivery system, and try to use it The next step will be to try to optimize Lon protease from protein structure, find its effective gene fragment, adopt mRNA delivery system, and try to use it as RNA targeting drug for drug optimization.
  
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</p>
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                            <!-- 插图 -->
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                            <div class="image-box">
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                            <figure><img src="https://static.igem.org/mediawiki/2021/8/84/T--CKWA-China--Desc1.jpeg" alt=""></figure>
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                            <p> </p>
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                        </div>
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                            <!-- 文字与图片排版 -->
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            <h3 class="point" id="point-2">Ref</h3>         
  
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                      <p>Butler, D.S.C., Cafaro, C., Putze, J. et al. A bacterial protease depletes c-MYC and increases survival in mouse models of bladder and colon cancer. Nat Biotechnol 39, 754–764 (2021). https://doi.org/10.1038/s41587-020-00805-3</p>
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                                <!-- 此处多加标题 每增加一个 href=#point-3(数字+1) -->
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                                <li class="active">
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                                    <a href="#point-1">1. A major problem in cancer.</a>
 +
                                    <ul class="sub-list">
 +
                                      <li><a href="#point-11">1.1 What are myc genes?</a></li>
 +
                                      <li><a href="#point-12">1.2 Impact of myc in cancer</a></li>
 +
                                      <li><a href="#point-13">1.3 C-myc genes</a></li>
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                                    </ul>
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                                </li>
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                                <li>
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                                  <a href="#point-2">2. New discoveries.</a>
 +
                                  <ul class="sub-list">
 +
                                      <li><a href="#point-21">2.1 Discovery of c-myc protein inhibition</a></li>
 +
                                      <li><a href="#point-22">2.2 Bacterial proteases deplete c-MYC</a></li>
 +
                                      <li><a href="#point-23">2.3 Literature conclusions</a></li>
 +
                                  </ul>
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                                </li>
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                                <li><a href="#point-3">3. Our solution</a></li>
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                            </ul>
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                        </div>
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                    </aside>
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Latest revision as of 09:21, 20 October 2021

Description

Description

1. A major problem in cancer.

Recurrence of cancer after treatment is a major problem facing humans today, and cancer recurrence is a great threat for cancer patients. Among them, overexpression of c-myc protein is closely related to this process, and patients with overactivation of c-myc gene in cancer cells tend to have poor prognosis.

1.1 What are myc genes?

myc genes have been shown to be a class of regulatory genes that are transcription factors that can encode myc-like proteins to become transcription factors transcription factors. they are essential for cell growth, metabolism and tissue development.

1.2 Impact of myc in cancer

They are essential for cell growth, metabolism and tissue development. Also, MYC is an important group of oncogenes. c-MYC is aberrantly expressed in about 30-50% of human malignancies and plays an extremely important role in affecting cell growth, proliferation, differentiation, apoptosis, as well as cell metabolism and malignant transformation. aberrantly high expression of c-MYC is associated with poor prognosis in many human cancers.

1.3 C-myc genes

The Myc family consists of three related human genes:
- c-myc (MYC), the first gene to be discovered in this family
- l-myc (MYCL)
- n-myc (MYCN)

2. New discoveries.

Scientists have recently discovered that a Lon protease from bacteria can effectively degrade c-myc protein, and this may be one of the reasons why bladder cancer patients with urinary tract infection with this bacteria have a better prognosis instead. Thus, Lon protein is expected to be a new macromolecular drug in the fight against cancer in humans.

2.1 Discovery of c-myc protein inhibition

MYC is important for the growth and development of the kidney. It has been shown that severe kidney infections impair kidney growth in childhood and that bladder cancer in patients with urinary tract infections instead has a better prognosis. Inference: MYC levels in the kidney may be disturbed by bacterial infections.

2.2 Bacterial proteases deplete c-MYC and increase survival in mouse models of bladder and colon cancer

Uropathogenic Escherichia coli (UPEC) can degrade c-MYC protein and attenuate MYC gene expression in human cells and animal tissues. Also, c-MYC protein can be degraded by cell-free Uropathogenic Escherichia coli (UPEC) lysates, as well as rapidly by purified Lon protease.

2.3 Literature conclusions

Lon protease from uropathogenic Escherichia coli (UPEC) is able to degrade c-MYC protein and attenuate MYC gene expression in human cells and animal tissues. Experiments in mouse models have shown that Lon protease delivered either intravesically or orally can delay tumor progression and improve survival in both MYC-dependent bladder and colon cancer models.

3. Our solution

We try to express Lon protease in various systems and test the yield of Lon protease in different environments. After achieving stable laboratory production, we will conduct in vitro cellular experiments to verify the inhibition ability of Lon protease on c-myc, and then we will try to optimize Lon protease from protein structure, find its effective gene fragment, adopt mRNA delivery system, and try to use it The next step will be to try to optimize Lon protease from protein structure, find its effective gene fragment, adopt mRNA delivery system, and try to use it as RNA targeting drug for drug optimization.

Ref

Butler, D.S.C., Cafaro, C., Putze, J. et al. A bacterial protease depletes c-MYC and increases survival in mouse models of bladder and colon cancer. Nat Biotechnol 39, 754–764 (2021). https://doi.org/10.1038/s41587-020-00805-3