Difference between revisions of "Team:Shanghai Metro/Partnership"

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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2021.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2021.igem.org/Judging/Awards"> award listed below</a>. </p>
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<h1>Partnership</h1>
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<h3>Gold Medal Criterion #5</h3>
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<p> Collaborate throughout the year with at least one other 2021 iGEM team on a set of shared objectives related to both of your projects. This partnership should go beyond a Silver medal collaboration.
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Please see the <a href="https://2021.igem.org/Judging/Medals">2021 Medals Page</a> for more information.
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        <div class="sub-title">Partnership </div>
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            <img src="https://static.igem.org/mediawiki/2021/0/09/T--Shanghai_Metro--partnership01.jpg" alt="" />
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        <div class="article-title">Background of Partnership </div>
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        <div class="article-content">PecTeast (Xiamen_city) is also a 2021 iGEM high school team. They focuses on
 +
            applying CRISPR-Cas 9 to
 +
            create a novel wine yeast, which is functional in both degrading pectin and fermenting. Currently, in fruit
 +
            wine and juice production, pectase is added to degrade pectin to enhance juice transparency and even the
 +
            overall production efficiency. Petcase usually costs 100-250 RMB/kg and it is estimated that it costs
 +
            100-1000 RMB to produce 1 ton of fruits. Thereafter, if team PecTeast’s experiments succeed, the
 +
            widely-application of their yeast will lower the production costs of fruit wines and juice. It also means
 +
            that the commercial values of their yeast is tremendous, thus team PecTeast came to us to discuss in-depth
 +
            cooperation.</div>
 +
        <div class="article-title">Overview of VI Patent Project (Strain Patent Protection Principles) </div>
 +
        <div class="article-content"><b>• </b>Originally-designed plasmids are transformed to E. Coli
 +
            (DH5a) to realize patent protection; </div>
 +
        <div class="article-content"><b>• </b>Options of originally-designed plasmids could be Pus232-ike2-tke2 or
 +
            Pus232-ike4-tke4;</div>
 +
        <div class="article-content"><b>• </b>Pus232 is extracted from E.Coli; gene ike and tke are extracted from
 +
            Pseudomonas putida;</div>
 +
        <div class="article-content"><b>• </b>Gene tke secretes toxin while gene ike produces protein immune to that
 +
            toxin; </div>
 +
        <div class="article-content"><b>• </b>The promoter of gene ike is inducible, indicating that only with the
 +
            induction of a specific ligand, will gene ike be expressed ;</div>
 +
        <div class="article-content"><b>• </b>The promoter of gene tke is constitutive, indicating that its toxic
 +
            protein expresses at any time. </div>
 +
        <div class="article-title">How to Protect Team PecTeast (Xiamen_city)’s Yeast through Partnership? </div>
 +
        <div class="article-content"><b>• </b>Extract gene ike2-tke2 or ike4-tke4 from originally-designed plasmids;
 +
        </div>
 +
        <div class="article-content"><b>• </b>Amplify the extracted genes by PCR;</div>
 +
        <div class="article-content"><b>• </b>Produce homologous recombination template by CRISPR, and then integrate
 +
            the extracted genes to the genome of team PecTeast’s yeast ;
 +
            (Since the effector of gene tke and immune factor of gene ike are commonly used, it is unnecessary to
 +
            re-design them)
 +
        </div>
 +
        <div class="article-content"><b>• </b>After the gene editing, put the yeast in a certain ligand to realize
 +
            patent protection.</div>
 +
        <div class="article-title">Mutual Benefits for VI Patent and PecTeast</div>
 +
        <div class="article-content"><b>• </b>Useful patent protection tools acquired by team PecTeast and further
 +
            value may be added to their yeast in commercialization;</div>
 +
        <div class="article-content"><b>• </b>Commercialization opportunities for team VI Patent, if the partnership
 +
            succeeds between PecTeast and VI Patent, this model could be proliferated fast to other iGEM teams,
 +
            enhancing the impact of team VI patent;</div>
 +
        <div class="article-content"><b>• </b>Demonstration of iGEM spirit-creating a mutually-beneficial, open, and
 +
            collaborated platform for synthetic biology and related projects.</div>
 +
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Latest revision as of 03:08, 20 October 2021

Shanghai_Metro

Partnership
Background of Partnership
PecTeast (Xiamen_city) is also a 2021 iGEM high school team. They focuses on applying CRISPR-Cas 9 to create a novel wine yeast, which is functional in both degrading pectin and fermenting. Currently, in fruit wine and juice production, pectase is added to degrade pectin to enhance juice transparency and even the overall production efficiency. Petcase usually costs 100-250 RMB/kg and it is estimated that it costs 100-1000 RMB to produce 1 ton of fruits. Thereafter, if team PecTeast’s experiments succeed, the widely-application of their yeast will lower the production costs of fruit wines and juice. It also means that the commercial values of their yeast is tremendous, thus team PecTeast came to us to discuss in-depth cooperation.
Overview of VI Patent Project (Strain Patent Protection Principles)
• Originally-designed plasmids are transformed to E. Coli (DH5a) to realize patent protection;
• Options of originally-designed plasmids could be Pus232-ike2-tke2 or Pus232-ike4-tke4;
• Pus232 is extracted from E.Coli; gene ike and tke are extracted from Pseudomonas putida;
• Gene tke secretes toxin while gene ike produces protein immune to that toxin;
• The promoter of gene ike is inducible, indicating that only with the induction of a specific ligand, will gene ike be expressed ;
• The promoter of gene tke is constitutive, indicating that its toxic protein expresses at any time.
How to Protect Team PecTeast (Xiamen_city)’s Yeast through Partnership?
• Extract gene ike2-tke2 or ike4-tke4 from originally-designed plasmids;
• Amplify the extracted genes by PCR;
• Produce homologous recombination template by CRISPR, and then integrate the extracted genes to the genome of team PecTeast’s yeast ; (Since the effector of gene tke and immune factor of gene ike are commonly used, it is unnecessary to re-design them)
• After the gene editing, put the yeast in a certain ligand to realize patent protection.
Mutual Benefits for VI Patent and PecTeast
• Useful patent protection tools acquired by team PecTeast and further value may be added to their yeast in commercialization;
• Commercialization opportunities for team VI Patent, if the partnership succeeds between PecTeast and VI Patent, this model could be proliferated fast to other iGEM teams, enhancing the impact of team VI patent;
• Demonstration of iGEM spirit-creating a mutually-beneficial, open, and collaborated platform for synthetic biology and related projects.

Contact Info.

Email: binghan423@163.com; runshigu@126.com

Wechat Official Account: IGEM VIPatent