The first generation
In order to make our engineering Chlamydomonas can be applied to the actual environment, we designed a device. The device consists of two transparent acrylic baskets inside and outside. The outer basket plays a protective role. The top of the inner frame is designed with a screw hole and a sampling hole. Screw holes are used to fix the inner and outer frames. The sampling hole allows us to take samples from the inside of the device to detect the environmental conditions in the inner frame. Algae with fixed cells are contained in the inner frame. A semi permeable membrane is installed outside the inner frame and only ions and small molecules are allowed to enter. At the same time, due to the pore size of cellulose acetate semi permeable membrane (5 μm) which is smaller than the cell size of Chlamydomonas reinhardtii (10 μm) it can also intercept Chlamydomonas reinhardtii , which is the second barrier for our biosafety. If our kill switch is implemented. We will also install near-infrared light filters outside the inner frame to filter the near-infrared light that causes light suicide. The whole device only needs a foam ring to float on the surface of water. The whole device is made of transparent materials to allow sunlight to enter and make algae grow healthily.
Figure 1. Three dimensional model of the device
Figure 2: physical structure of the device
The acrylic materials used in the whole device are also green and recyclable materials (Mao Xirong, 2009). Cellulose acetate membrane also has good degradability as a bio -based polymer membrane. Cellulose acetate membrane also has the advantages of high selectivity, high water permeability, non-toxic to organisms and difficult to be oxidized (Liu Ying et al., 2020).
Other Generation
References
- 毛锡荣. (2009). 家具设计中的材料——亚克力. 艺术与设计(理论) 2, 262-263.
- 刘莹, 孟凡浩, 刘钰娇, and 李奕霏. (2020). 醋酸纤维素复合膜的改性及应用进展. 塑料 49, 127-130.