Team:Qdai/Contribution
Contribution
We will add to the BBa_K1807002 page that BBa_K1807002 shows high phosphorus uptake when introduced into E. coli using the plasmid, and contribute to the igem team that will use this part in the future.
We constructed a plasmid (pBEST-ppk) containing BBa_K1807002 and measured the growth rate of bacteria by OD600 using medium with normal phosphorus concentration and medium with double phosphorus concentration.
After the OD600 readings reached a plateau, the increase in phosphorus uptake by pBEST-ppk was characterized by measuring the decrease in the amount of phosphorus in the medium using the QuantiChrom Phosphate Assay Kit (BioAssay Systems).
The results showed that pBEST-ppk showed high phosphorus uptake both in normal medium and in medium with twice the phosphorus concentration.
figure.1 Phosphorus uptakes (mg) in LB medium.
figure.2 Phosphorus uptakes (mg) in medium with twice the phosphorus concentration.
pBEST: control, pBEST-ppk: containing ppk. Each culture was 1.5 mL.