We used the following protocols to build our circuits, and each circuit has amazing results. You can click here to see the results.
(1) Conservation
(2) Semi-solid Agar Stab Strain Preservation Method
(3) Inoculation
(4) Plasmid Extraction
(1) Colony Polymerase Chain Reaction
(2) Ligase
(3) Gel Electrophoresis
(4) Gel Extraction
(5) Preparative Methods Of Competent Escherichia coli Cells
(6) Transformation Of Escherichia coli
(2) Gel Electrophoresis
(1) Fluorescence measurement